Pulsed Field Gel Electrophoresis

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Pulsed Field Gel Electrophoresis

Author : Bruce Birren,Eric Lai
Publisher : Academic Press
Page : 272 pages
File Size : 52,5 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323137621

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Pulsed Field Gel Electrophoresis by Bruce Birren,Eric Lai Pdf

Pulsed Field Gel Electrophoresis: A Practical Guide is the first laboratory manual to describe the theory and practice of this technique. Based on the authors' experience developing pulsed field gel instruments and teaching procedures, this book provides everything a researcher or student needs to know in order to understand and carry out pulsed field gel experiments. Clear, well-tested protocols assume only that users have a basic familiarity with molecular biology. Thorough coverage of useful data, theory, and applications ensures that this book is also a lasting resource for more advanced practitioners of pulsed field gels. Reviews all types of pulsed field gel electrophoresis Describes all commercially available systems and summarizes advantages and limitations of each Includes step-by-step protocols for sample preparation and analysis Illustrated with photographs that depict How to run gels: What the results should look like What they look like when they go wrong Covers applications to a wide range of organisms Includes bibliography of over 900 publications and cross-referenced by topic, application, and organism

Pulsed Field Gel Electrophoresis

Author : Anthony P. Monaco
Publisher : IRL Press
Page : 224 pages
File Size : 48,7 Mb
Release : 1995
Category : DNA
ISBN : UOM:39015037858076

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Pulsed Field Gel Electrophoresis by Anthony P. Monaco Pdf

Pulsed field gel electrophoresis (PFGE) has provided a very reliable system for separation of DNA fragments greater than 50 kb and has made a significant impact on the analysis of both prokaryotic and eukaryotic genomes. The chapters in this book cover both the theory behind this very important technique and present detailed protocols for many of its major uses. The first chapter describes the basis of PFGE with interesting new insights in modelling how PFGE separates large fragments of DNA. The next two describe the use of PFGE in constructing long range restriction site maps in mammalian genomic DNA and in the detection of gross rearrangements in the DNA of patients with Duchenne muscular dystrophy and Charcot-Marie-Tooth disease. There are four chapters dedicated to the use of PFGE in cloning form, construction of, analysis of, and transfer of yeast artificial chromosomes (YACs). The final two chapters describe the use of PFGE in the analysis of bacterial chromosomes and protozoan parasite chromosomes. This book will therefore be an essential resource book for researchers in laboratories that require PFGE in their research strategies, including those involved in molecular human genetics, mammalian genetics, molecular microbiology, molecular parasitology, biochemistry, and molecular diagnostics.

Pulsed-Field Gel Electrophoresis

Author : Margit Burmeister,Levy Ulanovsky
Publisher : Springer Science & Business Media
Page : 473 pages
File Size : 42,5 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592594993

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Pulsed-Field Gel Electrophoresis by Margit Burmeister,Levy Ulanovsky Pdf

Presents step-by-step protocols for users of Pulsed-Field Gel Electrophoresis (PFGE), whether novice or expert. This book features a wide range of PFGE techniques, auxiliary methods, and a diverse array of powerful applications. It includes protocols designed to work even the first time they are used.

Pulsed-field Gel Electrophoresis

Author : Anonim
Publisher : Unknown
Page : 86 pages
File Size : 48,7 Mb
Release : 1990
Category : Electronic
ISBN : OCLC:24874108

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Pulsed-field Gel Electrophoresis by Anonim Pdf

Pulsed Field Gel Electrophoresis

Author : Anthony P. Monaco
Publisher : Unknown
Page : 128 pages
File Size : 41,6 Mb
Release : 2001
Category : Electronic
ISBN : OCLC:255591612

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Pulsed Field Gel Electrophoresis by Anthony P. Monaco Pdf

Pulse Field Gel Electrophoresis

Author : Kieran Jordan,Marion Dalmasso
Publisher : Humana
Page : 0 pages
File Size : 42,8 Mb
Release : 2015-04-11
Category : Science
ISBN : 1493925989

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Pulse Field Gel Electrophoresis by Kieran Jordan,Marion Dalmasso Pdf

This volume will be of interest to epidemiologists, food microbiologists, and anyone working on comparing bacterial isolates. Pulse Field Gel Electrophoresis: Methods and Protocols guides readers through methods and protocols that will advance the harmonisation of PFGE methodologies and facilitate inter-laboratory comparisons of PFGE profiles from pathogenic and non-pathogenic bacteria. As a volume in the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls. Concise and easy-to-use, Pulse Field Gel Electrophoresis: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Pet-to-Man Travelling Staphylococci

Author : Vincenzo Savini
Publisher : Academic Press
Page : 322 pages
File Size : 48,8 Mb
Release : 2018-03-14
Category : Science
ISBN : 9780128135488

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Pet-to-Man Travelling Staphylococci by Vincenzo Savini Pdf

Pet-to-Man Travelling Staphylococci: A World in Progress explores Staphylococci, a dangerous pathogen that affects both humans and animals with a wide range of infection states. This bacteria can spread rapidly as a commensal organism in both humans and pets, and is an agent of disease. Staphylococci are potentially highly virulent pathogens which require urgent medical attention. In addition, Staphylococci remain a threat within hospital environments, where they can quickly spread across a patient population. This book explores the organisms' resistance to many compounds used to treat them, treatment failure and multidrug resistant staphylococci, amongst other related topics. Focuses not only on man and animal staphylococcal diseases, but on the role of shared household in man-to-pet (and vice versa) transmission Underlines the importance of professional exposure to mammals (i.e. veterinary and farm personnel) in the establishment of shared colonization's and related diseases Highlights the impact of shared staphylococci and virulence determinants in human and veterinary pathology Sheds light on the way staphylococci may be recognized in clinical laboratories

The Nucleic Acid Protocols Handbook

Author : Ralph Rapley
Publisher : Springer Science & Business Media
Page : 478 pages
File Size : 41,5 Mb
Release : 2008-06-29
Category : Science
ISBN : 9781592590384

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The Nucleic Acid Protocols Handbook by Ralph Rapley Pdf

A comprehensive treasury of all the key molecular biology methods-ranging from DNA extraction to gene localization in situ-needed to function effectively in the modern laboratory. Each of the 120 highly successful techniques follows the format of the much acclaimed Methods in Molecular BiologyOao series, providing an introduction to the scientific basis of each technique, a complete listing of all the necessary materials and reagents, and clear step-by-step instruction to permit error-free execution. Included for each technique are notes about pitfalls to avoid, troubleshooting tips, alternate methods, and explanations of the reasons for certain steps-all key elements contributing significantly to success or failure in the lab. The Nucleic Acid Protocols Handbook constitutes today's most comprehensive collection of all the key classic and cutting-edge techniques for the successful isolation, analysis, and manipulation of nucleic acids by both experienced researchers and those new to the field."

DNA Electrophoresis

Author : Katsuhiro Hanada
Publisher : Humana
Page : 0 pages
File Size : 52,5 Mb
Release : 2021-01-28
Category : Medical
ISBN : 1071603256

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DNA Electrophoresis by Katsuhiro Hanada Pdf

This volume explores the latest techniques used by researchers to study DNA electrophoresis, with focus on various species including bacteria, yeasts, and mammalian cells. The chapters in this book cover topics such as two-dimensional gel electrophoresis; DNA replication; pulsed-field gel electrophoresis; ChIP; and post-labeling/PAGE method for detection of DNA adducts. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, DNA Electrophoresis: Methods and Protocols is a valuable resource for any researchers looking to learn more about this developing field.

Mims' Medical Microbiology E-Book

Author : Richard Goering,Hazel Dockrell,Mark Zuckerman,Peter L. Chiodini
Publisher : Elsevier Health Sciences
Page : 580 pages
File Size : 48,6 Mb
Release : 2018-02-27
Category : Medical
ISBN : 9780702072024

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Mims' Medical Microbiology E-Book by Richard Goering,Hazel Dockrell,Mark Zuckerman,Peter L. Chiodini Pdf

Learn all the microbiology and basic immunology concepts you need to know for your courses and exams. Now fully revised and updated, Mims’ clinically relevant, systems-based approach and abundant colour illustrations make this complex subject easy to understand and remember. Learn about infections in the context of major body systems and understand why these are environments in which microbes can establish themselves, flourish, and give rise to pathologic changes. This systems-based approach to microbiology employs integrated and case-based teaching that places the ‘bug parade’ into a clinical context. Effectively review for problem-based courses with the help of chapter introductions and ‘Lessons in Microbiology’ text boxes that highlight the clinical relevance of the material, offer easy access to key concepts, and provide valuable review tools. Approach microbiology by body system or by pathogen through the accompanying electronic ‘Pathogen Parade’ – a quickly searchable, cross-referenced glossary of viruses, bacteria and fungi A new electronic ‘Vaccine Parade’ offers quick-reference coverage of the most commonly used vaccines in current clinical practice Deepen your understanding of epidemiology and the important role it plays in providing evidence-based identification of key risk factors for disease and targets for preventative medicine. Grasp and retain vital concepts easily, with a user-friendly colour coded format, succinct text, key concept boxes, and dynamic illustrations. New and enhanced information reflects the growing importance of the human microbiota and latest molecular approaches Access the complete contents on the go via the accompanying interactive eBook, with a range of bonus materials to enhance learning and retention – includes self-assessment materials and clinical cases to check your understanding and aid exam preparation.

Molecular Bacteriology

Author : Neil Woodford,Alan Johnson
Publisher : Springer Science & Business Media
Page : 704 pages
File Size : 44,5 Mb
Release : 1998-04-30
Category : Medical
ISBN : UOM:39015040158795

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Molecular Bacteriology by Neil Woodford,Alan Johnson Pdf

The enormous advances in molecular biology that have been witnessed in . Not recent years have had major impacts on many areas of the biological sciences least of these has been in the field of clinical bacteriology and infectious disease . Molecular Bacteriology: Protocols and ClinicalApplications aims to provide the reader with an insight into the role that molecular methodology has to play in modern medical bacteriology. The introductory chapter ofMolecular Bacteriology: ProtocolsandCli- cal Applications offers a personal overview by a Consultant Medical Microbio- gist of the impact and future potential offered by molecular methods. The next six chapters comprise detailed protocols for a range of such methods . We believe that the use of these protocols should allow the reader to establish the various methods described in his or her own laboratory. In selecting the methods to be included in this section, we have concentrated on those that, arguably, have greatest current relevance to reference clinical bacteriology laboratories; we have deliberately chosen not to give detailed protocols for certain methods, such as multilocus enzyme electrophoresis that, in our opinion, remain the preserve of specialist la- ratories and that are not currently suited for general use. We feel that the methods included in this section will find increasing use in diagnostic laboratories and that it is important that the concepts, advantages, and limitations of each are th- oughly understood by a wide range of workers in the field .

Practical Healthcare Epidemiology

Author : Ebbing Lautenbach,Preeti N. Malani,Keith F. Woeltje,Jennifer H. Han,Emily K. Shuman,Jonas Marschall
Publisher : Cambridge University Press
Page : 455 pages
File Size : 52,9 Mb
Release : 2018-04-19
Category : Medical
ISBN : 9781107153165

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Practical Healthcare Epidemiology by Ebbing Lautenbach,Preeti N. Malani,Keith F. Woeltje,Jennifer H. Han,Emily K. Shuman,Jonas Marschall Pdf

A clear, hands-on outline of best practices for infection prevention that directly improve patient outcomes across the healthcare continuum.

Genetics and Evolution of Infectious Diseases

Author : Michel Tibayrenc
Publisher : Elsevier
Page : 773 pages
File Size : 43,8 Mb
Release : 2010-12-17
Category : Medical
ISBN : 9780123848901

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Genetics and Evolution of Infectious Diseases by Michel Tibayrenc Pdf

Genetics and Evolution of Infectious Diseases is at the crossroads between two major scientific fields of the 21st century: evolutionary biology and infectious diseases. The genomic revolution has upset modern biology and has revolutionized our approach to ancient disciplines such as evolutionary studies. In particular, this revolution is profoundly changing our view on genetically driven human phenotypic diversity, and this is especially true in disease genetic susceptibility. Infectious diseases are indisputably the major challenge of medicine. When looking globally, they are the number one killer of humans and therefore the main selective pressure exerted on our species. Even in industrial countries, infectious diseases are now far less under control than 20 years ago. The first part of this book covers the main features and applications of modern technologies in the study of infectious diseases. The second part provides detailed information on a number of the key infectious diseases such as malaria, SARS, avian flu, HIV, tuberculosis, nosocomial infections and a few other pathogens that will be taken as examples to illustrate the power of modern technologies and the value of evolutionary approaches. Takes an integrated approach to infectious diseases Includes contributions from leading authorities Provides the latest developments in the field

Manual of Commercial Methods in Clinical Microbiology

Author : Anonim
Publisher : John Wiley & Sons
Page : 616 pages
File Size : 40,7 Mb
Release : 2016-03-28
Category : Science
ISBN : 9781119021865

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Manual of Commercial Methods in Clinical Microbiology by Anonim Pdf

The Manual of Commercial Methods in Clinical Microbiology 2nd Edition, International Edition reviews in detail the current state of the art in each of the disciplines of clinical microbiology, and reviews the sensitivities, specificities and predictive values, and subsequently the effectiveness, of commercially available methods – both manual and automated. This text allows the user to easily summarize the available methods in any particular field, or for a specific pathogen – for example, what to use for an Influenza test, a Legionella test, or what instrument to use for identification or for an antibiotic susceptibility test. The Manual of Commercial Methods in Clinical Microbiology, 2nd Edition, International Edition presents a wealth of relevant information to clinical pathologists, directors and supervisors of clinical microbiology, infectious disease physicians, point-of-care laboratories, professionals using industrial applications of diagnostic microbiology and other healthcare providers. The content will allow professionals to analyze all commercially available methods to determine which works best in their particular laboratory, hospital, clinic, or setting. Updated to appeal to an international audience, The Manual of Commercial Methods in Clinical Microbiology, 2nd Edition, International Edition is an invaluable reference to those in the health science and medical fields.

Methods in Practical Laboratory Bacteriology

Author : Henrik Chart
Publisher : CRC Press
Page : 174 pages
File Size : 51,6 Mb
Release : 1994-08-10
Category : Science
ISBN : 0849386926

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Methods in Practical Laboratory Bacteriology by Henrik Chart Pdf

The success of laboratory experiments relies heavily on the technical ability of the bench scientist, with the aid of "tricks-of-the-trade", to generate consistent and reliable data. Regrettably, however, these invaluable "tricks-of-the-trade" are frequently omitted from scientific publications. This paucity of practical information relating to the conduct of laboratory bacteriology experiments creates a gaping void in the pertinent literature. Methods in Practical Laboratory Bacteriology fills this void. It provides detailed technical information that ensures that you achieve consistent and reliable data. The book addresses the aspects of bacterial fractionation and membrane characterization, the analysis of Lipopolysaccharides and the techniques of SDS-PAGE, immunoblotting, and ELISA. It also describes the methods used for detecting and quantifying bacterial resistance to antibiotics, and the analysis of bacterial chromosomes by pulsed-field gel electrophoresis (PFGE). Methods in Practical Laboratory Bacteriology also covers protocols for extracting the fingerprinting plasmids, as well as the use of non-radio labeled gene probes and ribosomal RNA gene probes.