Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 44,8 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181
Molecular Biology of The Cell
Bruce Alberts
Membrane Protein Complexes
Membrane Protein Complexes Book in PDF, ePub and Kindle version is available to download in english. Read online anytime anywhere directly from your device. Click on the download button below to get a free pdf file of Membrane Protein Complexes book. This book definitely worth reading, it is an incredibly well-written.
Membrane Protein Complexes: Structure and Function
J. Robin Harris,Egbert J. Boekema
Author : J. Robin Harris,Egbert J. Boekema
Publisher : Springer
Page : 464 pages
File Size : 50,8 Mb
Release : 2018-02-20
Category : Science
ISBN : 9789811077579
Membrane Protein Complexes: Structure and Function by J. Robin Harris,Egbert J. Boekema Pdf
This edited book contains a compilation of 14 advanced academic chapters dealing with the structure and function of membrane protein complexes. This rapidly advancing important field of study closely parallels those on soluble protein complexes, and viral protein and nucleoprotein complexes. Diverse topics are included in this book, ranging from membrane–bound enzymes to ion channels, proton pumps and photosystems. Data from X-ray crystallography, cryo-electron microscopy and other biophysical and biochemical techniques are presented throughout the book. There is extensive use of colour figures of protein structures. Throughout the book structure and function are closely correlated. The two editors, Egbert Boekema and J. Robin Harris, have worked on aspects of membrane and soluble proteins throughout their scientific careers and also have much publishing experience. The Subcellular Biochemistry series has expanded considerably in recent years, including several related volumes. The theme of protein complexes will be continued within several future volumes, thereby creating encyclopaedic coverage. The chapter topics within this book are particularly relevant to those involved in the biological and biomedical sciences. It is aimed at the advanced undergraduates, postgraduates and established researchers within this broad field. It is hoped that the book will be of interest and use to those involved with the study of cellular membranes and their associated proteins.
Formation and Cooperative Behaviour of Protein Complexes on the Cell Membrane
Ksenia Guseva
Author : Ksenia Guseva
Publisher : Springer Science & Business Media
Page : 89 pages
File Size : 51,8 Mb
Release : 2011-10-01
Category : Science
ISBN : 9783642239885
Formation and Cooperative Behaviour of Protein Complexes on the Cell Membrane by Ksenia Guseva Pdf
With the aim of providing a deeper insight into possible mechanisms of biological self-organization, this thesis presents new approaches to describe the process of self-assembly and the impact of spatial organization on the function of membrane proteins, from a statistical physics point of view. It focuses on three important scenarios: the assembly of membrane proteins, the collective response of mechanosensitive channels and the function of the twin arginine translocation (Tat) system. Using methods from equilibrium and non-equilibrium statistical mechanics, general conclusions were drawn that demonstrate the importance of the protein-protein interactions. Namely, in the first part a general aggregation dynamics model is formulated, and used to show that fragmentation crucially affects the efficiency of the self-assembly process of proteins. In the second part, by mapping the membrane-mediated forces into a simplified many-body system, the dynamic and equilibrium behaviour of interacting mechanosensitive channels is derived, showing that protein agglomeration strongly impacts its desired function. The final part develops a model that incorporates both the agglomeration and transport function of the Tat system, thereby providing a comprehensive description of this self-organizing process.
A Practical Guide to Membrane Protein Purification
Gebhard von Jagow
Author : Gebhard von Jagow
Publisher : Elsevier
Page : 185 pages
File Size : 49,6 Mb
Release : 2013-10-22
Category : Science
ISBN : 9780080571720
A Practical Guide to Membrane Protein Purification by Gebhard von Jagow Pdf
A Practical Guide to Membrane Protein Purification is written especially for researchers who have some familarity with separation of water-soluble proteins, but who may not be aware of the pitfalls they face with membrane proteins. This guide presents techniques in a concise form, emphasizing the aspects unique to membrane proteins. The book explains the principles of the methods, permitting researchers and students new to this area to adapt these techniques to their particular needs. The second volume in the series, this book is an essential manual for investigations of structure and function of native membrane proteins, as well as for purification of these proteins for immunization and protein sequencing. Separation, Detection, and Characterization of Biological Macromolecules is a new series of laboratory guides. Each volume focuses on a topic of central interest to scientists and students in biomedical and biological research. Introductory chapters are followed by clear, step-by-step protocols that present principles and practice. These concise manuals are designed for optimal understanding of methods as well as for practical benchtop use. Provides general guidelines and strategies for isolation of membrane proteins Describes detailed practical procedures that have been the widest applications, and lowest specialized equipment needs Gives special emphasis to new native and denaturing electrophoresis techniques Explains modifications of techniques used for water-soluble proteins
Membrane Protein Protocols
Barry S. Selinsky
Author : Barry S. Selinsky
Publisher : Springer Science & Business Media
Page : 330 pages
File Size : 54,5 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592594009
Membrane Protein Protocols by Barry S. Selinsky Pdf
Knowledge of the three-dimensional structure of a protein is absolutely required for the complete understanding of its function. The spatial orientation of amino acids in the active site of an enzyme demonstrates how substrate specificity is defined, and assists the medicinal chemist in the design of s- cific, tight-binding inhibitors. The shape and contour of a protein surface hints at its interaction with other proteins and with its environment. Structural ana- sis of multiprotein complexes helps to define the role and interaction of each individual component, and can predict the consequences of protein mutation or conditions that promote dissociation and rearrangement of the complex. Determining the three-dimensional structure of a protein requires milligram quantities of pure material. Such quantities are required to refine crystallization conditions for X-ray analysis, or to overcome the sensitivity limitations of NMR spectroscopy. Historically, structural determination of proteins was limited to those expressed naturally in large amounts, or derived from a tissue or cell source inexpensive enough to warrant the use of large quantities of cells. H- ever, with the advent of the techniques of modern gene expression, many p- teins that are constitutively expressed in minute amounts can become accessible to large-scale purification and structural analysis.
Expression, Purification, and Structural Biology of Membrane Proteins
Camilo Perez,Timm Maier
Author : Camilo Perez,Timm Maier
Publisher : Humana
Page : 0 pages
File Size : 44,8 Mb
Release : 2020-02-29
Category : Science
ISBN : 1071603728
Expression, Purification, and Structural Biology of Membrane Proteins by Camilo Perez,Timm Maier Pdf
This book collects up-to-date advanced protocols and advice from leading experts in the area of membrane protein biology that can be applied to structural and functional studies of any membrane protein system. The contents explore methods for cloning and expression of membrane proteins and membrane protein complexes in prokaryotic and eukaryotic systems, approaches for protein purification, nanobody applications, as well as biophysical characterization and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Expression, Purification, and Structure Biology of Membrane Proteins serves to guide and encourage young researchers and newcomers to the field to tackle bold new studies on membrane proteins. Chapter 11 is available open access under a CC-BY 4.0 license via link.springer.com.
Membrane Protein Purification and Crystallization
Carola Hunte,Gebhard von Jagow,Hermann Schagger
Author : Carola Hunte,Gebhard von Jagow,Hermann Schagger
Publisher : Elsevier
Page : 339 pages
File Size : 47,9 Mb
Release : 2003-01-05
Category : Science
ISBN : 9780080536170
Membrane Protein Purification and Crystallization by Carola Hunte,Gebhard von Jagow,Hermann Schagger Pdf
This second edition of Membrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins. Provides general guidelines and strategies for isolation and crystallization of membrane proteins Gives detailed protocols that have wide application, and low specialized equipment needs Emphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexes Gives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallization Presents a comprehensive guide to 2D-crystallization of membrane proteins
Formation and Cooperative Behaviour of Protein Complexes on the Cell Membrane
Anonim
Author : Anonim
Publisher : Unknown
Page : 80 pages
File Size : 47,6 Mb
Release : 2012
Category : Electronic
ISBN : OCLC:915437594
Formation and Cooperative Behaviour of Protein Complexes on the Cell Membrane by Anonim Pdf
Membrane Protein Structure and Function Characterization
Jean-Jacques Lacapere
Author : Jean-Jacques Lacapere
Publisher : Humana Press
Page : 419 pages
File Size : 40,9 Mb
Release : 2017-08-10
Category : Science
ISBN : 1493971492
Membrane Protein Structure and Function Characterization by Jean-Jacques Lacapere Pdf
In this present volume, different approaches are detailed to produce membrane proteins, purify them, study their function, determine their structure, and model them in membrane. Since every membrane protein behaves mostly in a unique way /fashion, knowledge of guidelines and tricks may help to increase chances to express, purify and characterize a peculiar membrane protein. Production of correctly folded protein remains a challenge. Moreover, getting a functional and stable protein requires to optimize membrane mimicking environments that can be detergent or artificial membranes. In some cases, the finding of the correct ligand which will stabilize the desired conformation is needed. In other cases, stabilization can be obtained using specific antibodies. This volume also presents different techniques to analyze the functional status of membrane proteins. Written in the highly successful Methods in Molecular Biology series format, chapters in Membrane Protein Structure and Function Characterization: Methods and Protocols provide different techniques to analyze the functional and structural status of membrane proteins. Chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Membrane Protein Structure and Function Characterization: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Membrane Protein Structure
Stephen H White
Author : Stephen H White
Publisher : Springer
Page : 403 pages
File Size : 47,7 Mb
Release : 2013-05-27
Category : Science
ISBN : 9781461475156
Membrane Protein Structure by Stephen H White Pdf
Studies of receptors, ion channels, and other membrane proteins require a solid understanding of the structural principles of these important biomolecules. Membrane protein structure is, however, a very challenging field. The structures of only three types of transmembrane proteins have been determined to moderate or high resolution during the last two decades, a period during which the amino acid sequences of hundreds, if not thousands, of membrane proteins have been reported. As a result, the creation of structural models to serve as guides for studies of receptors, channels, and other membrane proteins has become crucially important. This book has been assembled in order to share the experiences and findings of expert researchers in protein structure and structure-prediction methods as well as membrane biophysics and lipid physical chemistry, whose work establishes the basis for the development of suitable model structures. The reviews presented here emphasize fundamental ideas and provide an entry to the diverse and complex literature. The four major sections deal with the general nature of the membrane protein structure problem, biochemical and molecular biological approaches to protein topology, direct structural methods, and model and physicochemical approaches. The work will be of interest to physiologists, cellular and molecular biologists, biophysicists, and biochemists working on the function of membrane proteins such as receptors, ion channels, and transporters, as well as senior graduate students and independent investigators.
Proteomics for Biological Discovery
Timothy D. Veenstra,John R. Yates, III
Author : Timothy D. Veenstra,John R. Yates, III
Publisher : John Wiley & Sons
Page : 361 pages
File Size : 42,7 Mb
Release : 2006-06-12
Category : Science
ISBN : 9780470007730
Proteomics for Biological Discovery by Timothy D. Veenstra,John R. Yates, III Pdf
Written by recognized experts in the study of proteins, Proteomics for Biological Discovery begins by discussing the emergence of proteomics from genome sequencing projects and a summary of potential answers to be gained from proteome-level research. The tools of proteomics, from conventional to novel techniques, are then dealt with in terms of underlying concepts, limitations and future directions. An invaluable source of information, this title also provides a thorough overview of the current developments in post-translational modification studies, structural proteomics, biochemical proteomics, microfabrication, applied proteomics, and bioinformatics relevant to proteomics. Presents a comprehensive and coherent review of the major issues faced in terms of technology development, bioinformatics, strategic approaches, and applications Chapters offer a rigorous overview with summary of limitations, emerging approaches, questions, and realistic future industry and basic science applications Discusses higher level integrative aspects, including technical challenges and applications for drug discovery Accessible to the novice while providing experienced investigators essential information Proteomics for Biological Discovery is an essential resource for students, postdoctoral fellows, and researchers across all fields of biomedical research, including biochemistry, protein chemistry, molecular genetics, cell/developmental biology, and bioinformatics.
Biological Small Angle Scattering: Techniques, Strategies and Tips
Barnali Chaudhuri,Inés G. Muñoz,Shuo Qian,Volker S. Urban
Author : Barnali Chaudhuri,Inés G. Muñoz,Shuo Qian,Volker S. Urban
Publisher : Springer
Page : 268 pages
File Size : 50,7 Mb
Release : 2017-12-07
Category : Science
ISBN : 9789811060380
Biological Small Angle Scattering: Techniques, Strategies and Tips by Barnali Chaudhuri,Inés G. Muñoz,Shuo Qian,Volker S. Urban Pdf
This book provides a clear, comprehensible and up-to-date description of how Small Angle Scattering (SAS) can help structural biology researchers. SAS is an efficient technique that offers structural information on how biological macromolecules behave in solution. SAS provides distinct and complementary data for integrative structural biology approaches in combination with other widely used probes, such as X-ray crystallography, Nuclear magnetic resonance, Mass spectrometry and Cryo-electron Microscopy. The development of brilliant synchrotron small-angle X-ray scattering (SAXS) beam lines has increased the number of researchers interested in solution scattering. SAS is especially useful for studying conformational changes in proteins, highly flexible proteins, and intrinsically disordered proteins. Small-angle neutron scattering (SANS) with neutron contrast variation is ideally suited for studying multi-component assemblies as well as membrane proteins that are stabilized in surfactant micelles or vesicles. SAS is also used for studying dynamic processes of protein fibrillation in amyloid diseases, and pharmaceutical drug delivery. The combination with size-exclusion chromatography further increases the range of SAS applications. The book is written by leading experts in solution SAS methodologies. The principles and theoretical background of various SAS techniques are included, along with practical aspects that range from sample preparation to data presentation for publication. Topics covered include techniques for improving data quality and analysis, as well as different scientific applications of SAS. With abundant illustrations and practical tips, we hope the clear explanations of the principles and the reviews on the latest progresses will serve as a guide through all aspects of biological solution SAS. The scope of this book is particularly relevant for structural biology researchers who are new to SAS. Advanced users of the technique will find it helpful for exploring the diversity of solution SAS methods and applications. Chapter 3 of this book is available open access under a CC BY 4.0 license at link.springer.com.
Macromolecular Protein Complexes II: Structure and Function
J. Robin Harris,Jon Marles-Wright
Author : J. Robin Harris,Jon Marles-Wright
Publisher : Springer Nature
Page : 657 pages
File Size : 54,7 Mb
Release : 2020-01-14
Category : Science
ISBN : 9783030281519
Macromolecular Protein Complexes II: Structure and Function by J. Robin Harris,Jon Marles-Wright Pdf
This book follows on from Volume 83 in the SCBI series (“Macromolecular Protein Complexes”), and addresses several important topics (such as the Proteasome, Anaphase Promoting Complex, Ribosome and Apoptosome) that were not previously included, together with a number of additional exciting topics in this rapidly expanding field of study. Although the first SCBI Protein Complex book focused on soluble protein complexes, the second (Vol. 87)addressed Membrane Complexes, and the third (Vol. 88) put the spotlight on Viral Protein and Nucleoprotein Complexes, a number of membrane, virus and even fibrillar protein complexes have been be considered for inclusion in the present book. A further book is also under preparation that follows the same pattern, in an attempt to provide a thorough coverage of the subject. Chapter 9 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.
Crystallization of Membrane Proteins
Hartmut Michel
Author : Hartmut Michel
Publisher : CRC Press
Page : 376 pages
File Size : 51,8 Mb
Release : 2018-01-18
Category : Medical
ISBN : 9781351088176
Crystallization of Membrane Proteins by Hartmut Michel Pdf
The precise knowledge of the structure of biological macromolecules forms the basis of understanding their function and their mechanism of action. It also lays the foundation for rational protein and drug design. The only method to obtain this knowledge is still crystallography. At present, the structures of about 400 proteins are known at or nearly at atomic proteins. However, only two of them are membrane proteins or complexes of the membrane proteins. The reasons for the difference is not the crystals of membrane proteins resists forming special problems when being analysed. The reason is that the membrane proteins resist into forming into well-ordered crystals. The intention of this book is to help to produce well-ordered crystals proteins and to provide guidelines, it is aimed at both biochemists and protein crystallographer‘s.
Membrane Protein Crystallization
Anonim
Author : Anonim
Publisher : Academic Press
Page : 336 pages
File Size : 47,6 Mb
Release : 2009-05-29
Category : Science
ISBN : 0080961592
Membrane Protein Crystallization by Anonim Pdf
This volume of Current Topics in Membranes focuses on Membrane Protein Crystallization, beginning with a review of past successes and general trends, then further discussing challenges of mebranes protein crystallization, cell free production of membrane proteins and novel lipids for membrane protein crystallization. This publication also includes tools to enchance membrane protein crystallization, technique advancements, and crystallization strategies used for photosystem I and its complexes, establishing Membrane Protein Crystallization as a needed, practical reference for researchers.
