Proteomics

Author : Lucio Comai,Jonathan E. Katz,Parag Mallick
Publisher : Humana
Page : 0 pages
File Size : 48,9 Mb
Release : 2017-02-11
Category : Science
ISBN : 1493967452

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Proteomics by Lucio Comai,Jonathan E. Katz,Parag Mallick Pdf

This volume aims to provide protocols on a wide range of biochemical methods, analytical approaches, and bioinformatics tools developed to analyze the proteome. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Proteomics: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Author : Daniel Liebler
Publisher : Springer Science & Business Media
Page : 210 pages
File Size : 51,5 Mb
Release : 2001-12-04
Category : Medical
ISBN : 9780896039919

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Introduction to Proteomics by Daniel Liebler Pdf

Daniel C. Liebler masterfully introduces the science of proteomics by spelling out the basics of how one analyzes proteins and proteomes, and just how these approaches are then employed to investigate their roles in living systems. He explains the key concepts of proteomics, how the analytical instrumentation works, what data mining and other software tools do, and how these tools can be integrated to study proteomes. Also discussed are how protein and peptide separation techniques are applied in proteomics, how mass spectrometry is used to identify proteins, and how data analysis software enables protein identification and the mapping of modifications. In addition, there are proteomic approaches for analyzing differential protein expression, characterizing proteomic diversity, and dissecting protein-protein interactions and networks.

Author : Daniela Cecconi
Publisher : Unknown
Page : 326 pages
File Size : 41,7 Mb
Release : 2021
Category : Proteomics
ISBN : 1071616412

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Proteomics Data Analysis by Daniela Cecconi Pdf

This thorough book collects methods and strategies to analyze proteomics data. It is intended to describe how data obtained by gel-based or gel-free proteomics approaches can be inspected, organized, and interpreted to extrapolate biological information. Organized into four sections, the volume explores strategies to analyze proteomics data obtained by gel-based approaches, different data analysis approaches for gel-free proteomics experiments, bioinformatic tools for the interpretation of proteomics data to obtain biological significant information, as well as methods to integrate proteomics data with other omics datasets including genomics, transcriptomics, metabolomics, and other types of data. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that will ensure high quality results in the lab. Authoritative and practical, Proteomics Data Analysis serves as an ideal guide to introduce researchers, both experienced and novice, to new tools and approaches for data analysis to encourage the further study of proteomics.

Author : Rune Matthiesen
Publisher : Unknown
Page : 405 pages
File Size : 52,8 Mb
Release : 2013
Category : Mass spectrometry
ISBN : 1627033920

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Mass Spectrometry Data Analysis in Proteomics by Rune Matthiesen Pdf

Since the publishing of the first edition, the methodologies and instrumentation involved in the field of mass spectrometry-based proteomics has improved considerably. Fully revised and expanded, Mass Spectrometry Data Analysis in Proteomics, Second Edition presents expert chapters on specific MS-based methods or data analysis strategies in proteomics. The volume covers data analysis topics relevant for quantitative proteomics, post translational modification, HX-MS, glycomics, and data exchange standards, among other topics. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Updated and authoritative, Mass Spectrometry Data Analysis in Proteomics, Second Edition serves as a detailed guide for all researchers seeking to further our knowledge in the field of proteomics.

Author : Timothy D. Veenstra,John R. Yates, III
Publisher : John Wiley & Sons
Page : 361 pages
File Size : 48,8 Mb
Release : 2006-06-12
Category : Science
ISBN : 9780470007730

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Proteomics for Biological Discovery by Timothy D. Veenstra,John R. Yates, III Pdf

Written by recognized experts in the study of proteins, Proteomics for Biological Discovery begins by discussing the emergence of proteomics from genome sequencing projects and a summary of potential answers to be gained from proteome-level research. The tools of proteomics, from conventional to novel techniques, are then dealt with in terms of underlying concepts, limitations and future directions. An invaluable source of information, this title also provides a thorough overview of the current developments in post-translational modification studies, structural proteomics, biochemical proteomics, microfabrication, applied proteomics, and bioinformatics relevant to proteomics. Presents a comprehensive and coherent review of the major issues faced in terms of technology development, bioinformatics, strategic approaches, and applications Chapters offer a rigorous overview with summary of limitations, emerging approaches, questions, and realistic future industry and basic science applications Discusses higher level integrative aspects, including technical challenges and applications for drug discovery Accessible to the novice while providing experienced investigators essential information Proteomics for Biological Discovery is an essential resource for students, postdoctoral fellows, and researchers across all fields of biomedical research, including biochemistry, protein chemistry, molecular genetics, cell/developmental biology, and bioinformatics.

Author : John M. Walker
Publisher : Springer Science & Business Media
Page : 969 pages
File Size : 49,5 Mb
Release : 2007-10-09
Category : Science
ISBN : 9781592598908

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The Proteomics Protocols Handbook by John M. Walker Pdf

Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein. The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. All laboratory-based protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Author : Oscar Alzate
Publisher : CRC Press
Page : 354 pages
File Size : 54,8 Mb
Release : 2009-10-26
Category : Science
ISBN : 1420076264

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Neuroproteomics by Oscar Alzate Pdf

In this, the post-genomic age, our knowledge of biological systems continues to expand and progress. As the research becomes more focused, so too does the data. Genomic research progresses to proteomics and brings us to a deeper understanding of the behavior and function of protein clusters. And now proteomics gives way to neuroproteomics as we begin to unravel the complex mysteries of neurological diseases that less than a generation ago seemed opaque to our inquiries, if not altogether intractable. Edited by Dr. Oscar Alzate, Neuroproteomics is the newest volume in the CRC Press Frontiers of Neuroscience Series. With an extensive background in mathematics and physics, Dr. Alzate exemplifies the newest generation of biological systems researchers. He organizes research and data contributed from all across the world to present an overview of neuroproteomics that is practical and progressive. Bolstered by each new discovery, researchers employing multiple methods of inquiry gain a deeper understanding of the key biological problems related to brain function, brain structure, and the complexity of the nervous system. This in turn is leading to new understanding about diseases of neurological deficit such as Parkinson’s and Alzheimer’s. Approaches discussed in the book include mass spectrometry, electrophoresis, chromatography, surface plasmon resonance, protein arrays, immunoblotting, computational proteomics, and molecular imaging. Writing about their own work, leading researchers detail the principles, approaches, and difficulties of the various techniques, demonstrating the questions that neuroproteomics can answer and those it raises. New challenges wait, not the least of which is the identification of potential methods to regulate the structures and functions of key protein interaction networks. Ultimately, those building on the foundation presented here will advance our understanding of the brain and show us ways to abate the suffering caused by neurological and mental diseases.

Author : Richard Twyman
Publisher : Garland Science
Page : 266 pages
File Size : 44,6 Mb
Release : 2004-05-01
Category : Science
ISBN : 9780203507391

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Principles of Proteomics by Richard Twyman Pdf

Principles of Proteomics is designed specifically to explain the different stages of proteomic analysis, their complexities and their jargon to students and researchers in a non-technical overview of the field. The author describes the broad range of problems which proteomics can address, including structural proteomics, interaction proteomics, protein modification analysis and functional proteomics. Methodologies are described in user-friendly language, from the more traditional two-dimensional gel electrophoresis to the new developments in protein chip technologies. These are well presented in the context of overall strategies which can be adopted to address the different aspects of large-scale protein analysis.

Author : Hamid Mirzaei,Martin Carrasco
Publisher : Springer
Page : 533 pages
File Size : 49,8 Mb
Release : 2016-12-14
Category : Science
ISBN : 9783319414485

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Modern Proteomics – Sample Preparation, Analysis and Practical Applications by Hamid Mirzaei,Martin Carrasco Pdf

This volume serves as a proteomics reference manual, describing experimental design and execution. The book also shows a large number of examples as to what can be achieved using proteomics techniques. As a relatively young area of scientific research, the breadth and depth of the current state of the art in proteomics might not be obvious to all potential users. There are various books and review articles that cover certain aspects of proteomics but they often lack technical details. Subject specific literature also lacks the broad overviews that are needed to design an experiment in which all steps are compatible and coherent. The objective of this book was to create a proteomics manual to provide scientists who are not experts in the field with an overview of: 1. The types of samples can be analyzed by mass spectrometry for proteomics analysis. 2. Ways to convert biological or ecological samples to analytes ready for mass spectral analysis. 3. Ways to reduce the complexity of the proteome to achieve better coverage of the constituent proteins. 4. How various mass spectrometers work and different ways they can be used for proteomics analysis 5. The various platforms that are available for proteomics data analysis 6. The various applications of proteomics technologies in biological and medical sciences This book should appeal to anyone with an interest in proteomics technologies, proteomics related bioinformatics and proteomics data generation and interpretation. With the broad setup and chapters written by experts in the field, there is information that is valuable for students as well as for researchers who are looking for a hands on introduction into the strengths, weaknesses and opportunities of proteomics.

Author : Salvatore Sechi
Publisher : Methods in Molecular Biology
Page : 322 pages
File Size : 42,7 Mb
Release : 2018-04-15
Category : Science
ISBN : 1493980661

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Quantitative Proteomics by Mass Spectrometry by Salvatore Sechi Pdf

This volume describes prominent methodologies developed by laboratories that have been leading the field of quantitative proteomics by mass spectrometry. The procedures for performing the experiments are described in an easy-to-understand manner with many technical details that usually are not reported in typical research articles. This second edition of Quantitative Proteomics by Mass Spectrometry provides a broad perspective of the methodologies used for quantifying proteins and post-translational modifications in different types of biomedical specimens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and thorough, Quantitative Proteomics by Mass Spectrometry, Second Edition is a valuable resource to help researchers understand and learn about the latest tools used in the study of quantitative proteomics by mass spectrometry.

Author : Nawin C. Mishra
Publisher : John Wiley & Sons
Page : 202 pages
File Size : 50,8 Mb
Release : 2011-09-19
Category : Science
ISBN : 9780470920848

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Introduction to Proteomics by Nawin C. Mishra Pdf

Proteomics provides an introductory insight on proteomics, discussing the basic principles of the field, how to apply specific technologies and instrumentation, and example applications in human health and diseases. With helpful study questions, this textbook presents an easy to grasp and solid overview and understanding of the principles, guidelines, and especially the complex instrumentation operations in proteomics for new students and research scientists. Written by a leader in proteomics studies, Proteomics offers an expert perspective on the field and the future of proteomics.

Author : Visith Thongboonkerd
Publisher : Springer Science & Business Media
Page : 533 pages
File Size : 42,6 Mb
Release : 2008-01-23
Category : Science
ISBN : 9781597454322

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Proteomics of Human Body Fluids by Visith Thongboonkerd Pdf

This volume is the first collection of applications of proteomics to analyze various human body fluids. Proteomics of Human Bodyfluids consists of two parts. The first provides basic principles and strategies for proteomic analysis of human body fluids. The second offers more details regarding methodologies and recent findings and clinical applications of each specific type of human body fluids.

Author : Jesus V Jorrin-Novo,Luis Valledor,Mari Angeles Castillejo,Maria-Dolores Rey
Publisher : Humana
Page : 417 pages
File Size : 43,5 Mb
Release : 2021-06-11
Category : Electronic
ISBN : 1071605305

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Plant Proteomics by Jesus V Jorrin-Novo,Luis Valledor,Mari Angeles Castillejo,Maria-Dolores Rey Pdf

Author : Werner Dubitzky,Martin Granzow,Daniel P. Berrar
Publisher : Springer Science & Business Media
Page : 300 pages
File Size : 52,5 Mb
Release : 2007-04-13
Category : Science
ISBN : 9780387475097

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Fundamentals of Data Mining in Genomics and Proteomics by Werner Dubitzky,Martin Granzow,Daniel P. Berrar Pdf

This book presents state-of-the-art analytical methods from statistics and data mining for the analysis of high-throughput data from genomics and proteomics. It adopts an approach focusing on concepts and applications and presents key analytical techniques for the analysis of genomics and proteomics data by detailing their underlying principles, merits and limitations.

Author : Xing Wang,Matthew Kuruc
Publisher : Humana Press
Page : 477 pages
File Size : 43,5 Mb
Release : 2018-10-01
Category : Science
ISBN : 1493988131

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Functional Proteomics by Xing Wang,Matthew Kuruc Pdf

This book seeks to fill in the current technology gap with a specific collection of technologies developed for the study of protein function at a proteome scale. Chapters explore topics from protein functions to other aspects of protein analysis, especially in post-translational modification, as most proteomes use this mechanism in some capacity to carry out their unique role in cellular regulation. By comparing functional proteomes, this presents a bridge to other levels of system biology research including genomics and metabolomics in order to provide readers with a relatively complete picture for how one might study the biological system of their interest. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Functional Proteomics: Methods and Protocols collects these novel technologies in the hope that new frontiers in biological research will be created, important drug targets can be identified, and clinically validated biomarkers and diagnostic tests can be further developed.