Author : Geoffrey Cohen
Publisher : Unknown
Page : 445 pages
File Size : 42,5 Mb
Release : 2017-10-20
Category : Electronic
ISBN : 1549980165
8 Day Kidney Preservation by Geoffrey Cohen Pdf
This book describes a system that was developed between 1975 and 1979 that for the first time succeeded in the preservation of kidneys in vitro for up to 8 days. The system used hypothermic perfusion with a Human Albumin (Plasma Protein Fraction) based perfusate. The study was presented to the University of Liverpool in 1983 as a thesis for the degree of ChM. This book includes the original text and the experimental logs for the study. Both glomerular and tubular injury occurred during storage and this progressed after re-implantation in non viable kidneys. Later work suggested that the concentration of Octanoic Acid in the Plasma Protein Fraction was the most important variable in this experimental system. Bottles of two versions of Plasma Protein Fraction are shown on the first page; the bottle on the left containing 3.6 mmol/l of Octanoic Acid is an example of the batches that were used to make the perfusate for successful 8 day preservation. The bottle on the right is from later production and contains 7.2 mmol/l of Octanoic Acid; no successful preservation beyond 5 days was achieved when this was used to make the perfusate. The success of long duration storage made it possible to show that measurement of the lysosomal enzyme NAG (N-Acetyl-glucosaminidase) in the perfusate could not be used to predict whether stored kidneys that had not been warm injured would function immediately. However, it was shown that perfusate NAG levels might be used to assess the severity of any warm injury.
