Advanced Technologies For Protein Complex Production And Characterization

Author : M. Cristina Vega,Francisco J. Fernández
Publisher : Springer Nature
Page : 217 pages
File Size : 50,6 Mb
Release : 2024
Category : Biochemistry
ISBN : 9783031521935

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Advanced Technologies for Protein Complex Production and Characterization by M. Cristina Vega,Francisco J. Fernández Pdf

Zusammenfassung: This edited volume discusses the identification, discovery, characterization, structure determination and modeling of multicomponent macromolecular complexes, and as such, it fully complements the first volume (ISBN 978-3-319-27214-6), which targeted methods of recombinant production of protein complexes. This book is divided in 8 sections offering a selection of technologies widely used in the characterization of protein and protein-nucleic acid complexes for different purposes and at different scales. From native electrophoresis methods, that are accessible to any reasonably well-equipped laboratory, to the sophisticated setup required for structure determination by cryo-electron microscopy or X-ray crystallography, this book contains a wide variety of clearly explained analytic and preparative approaches, connected with the production techniques developed on the previous volume. The readers will find an integral connection between this book and the first volume, which ensures a comprehensive and updated discussion of the main topics of the discipline. Taken together, these volumes constitute a cohesive and authoritative source of the research on multicomponent macromolecular complexes. In here, we focus on characterization of protein complexes in the broadest sense, which is not typically covered in other sources. Moreover, all chapters are carefully written by world renowned scientists and active researchers, making this volume ideal, not only as a reference source, but also as a companion book for the daily laboratory work. This book is aimed for a wide range of scientists, from science students to experienced researchers, working on protein and protein-nucleic acid complexes, who need a thorough understanding of protein production and complex characterization.

Author : M. Cristina Vega
Publisher : Springer
Page : 382 pages
File Size : 48,8 Mb
Release : 2016-05-10
Category : Science
ISBN : 9783319272160

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Advanced Technologies for Protein Complex Production and Characterization by M. Cristina Vega Pdf

This book presents advanced expression technologies for the production of protein complexes. Since complexes lie at the heart of modern biology, the expression, purification, and characterization of large amounts of high-quality protein complexes is crucial for the fields of biomedicine, biotechnology, and structural biology. From co-expression in E. coli, yeast, mammalian and insect cells to complex reconstitution from individual subunits, this book offers useful insights and guidance for successful protein expressionists. Across several sections readers will discover existing opportunities for the production of protein complexes in bacterial systems (including membrane proteins and cell-free co-expression), methylotrophic and non-methylotrophic yeasts, protozoa (Leishmania terantolae and Dictyostelium discoideum), baculovirus-infected insect cells, mammalian cells, plants and algae. Complex reconstitution from individually purified subunits or subcomplexes is discussed as a complementary strategy. A last section introduces briefly some of the biophysical and structural characterization techniques for macromolecular complexes using state-of-the-art solution scattering and nuclear magnetic resonance. This work is a guided tour over some of the most powerful and successful protein expression technologies, with a focus on co-expression and high-throughput applications. It is addressed to everyone interested in the production and characterization of macromolecular complexes, from university students who want an accessible description of the major co-expression systems to researchers in biomedicine and the life sciences seeking for an up-to-date survey of available technologies.

Author : Deepti Yadav,Abhishek Guldhe,Tukayi Kudanga
Publisher : Elsevier
Page : 0 pages
File Size : 43,8 Mb
Release : 2024-10-01
Category : Science
ISBN : 9780323985840

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Fundamentals of Recombinant Protein Production, Purification and Characterization by Deepti Yadav,Abhishek Guldhe,Tukayi Kudanga Pdf

Fundamentals of Recombinant Protein Production, Purification and Characterization is organized into nine chapters in a logical fashion that cover an introduction to recombinant proteins and expression in different host expression systems, extraction, purification and analysis of proteins. This important reference features protocols, along with the advantages and disadvantage of each expression hosts and characterization technique (presented in tabular format) and offers detailed coverage of all aspects of protein production and processing (upstream and downstream processing) in one place. Finally, the book ends with different characterization techniques. Production of recombinant proteins for biotechnological and therapeutic applications at a large scale is an essential need of mankind. With the huge application potential of therapeutic and industrial proteins, there has been increasing demand for effective and efficient bioprocessing strategies. Recent progress around recombinant DNA technologies and bioprocessing strategies has paved the way for efficient production of recombinant proteins. Important factors such as insolubility and cost of production need to be considered for large scale production of these recombinant proteins. Includes step-by-step reproducible protocols while also providing updated information on the rationale and latest developments in expression systems Can also be used as a handbook for protein expression and purification as expression systems and chromatographic methods are explained in detail Consists of notes on troubleshooting from the eminent researchers in the field Provides comprehensive information on protein production, purification and characterization in a single volume Describes different purification methods for comparatively difficult to obtain proteins Brings the topics of recombinant protein expression, purification and characterization together, thereby making it the first resource on how to solve problems with respect to upstream and downstream processing of heterologous proteins

Author : Vipin Chandra Kalia,Adesh Kumar Saini
Publisher : Springer
Page : 412 pages
File Size : 41,6 Mb
Release : 2017-10-07
Category : Medical
ISBN : 9789811055119

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Metabolic Engineering for Bioactive Compounds by Vipin Chandra Kalia,Adesh Kumar Saini Pdf

This book comprehensively discusses the latest research in the area of metabolic engineering. Metabolic engineering solutions for bioactive compounds are now being derived by means of heterologous gene expression, in a wide range of organisms. The book provides an overview of the model systems being employed for metabolic manipulation to yield bioactive molecules, such as single-cell proteins, antibody generation, metabolites, proteases, chaperones, therapeutic proteins, nanomaterials, polymeric conjugates, dendrimers and nanoassemblies, Escherichia coli, Agrobacterium, Saccharomyces cerevisiae and cell lines, etc. In addition, it shares insights into the scope of these methods in the areas of prevention, diagnosis and treatment of diseases, e.g. immunotherapy for curing various diseases like cancer, allergies, autoimmune diseases, etc.

Author : Renaud Vincentelli
Publisher : Unknown
Page : 537 pages
File Size : 45,9 Mb
Release : 2019
Category : Biochemistry
ISBN : 149399624X

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High-Throughput Protein Production and Purification by Renaud Vincentelli Pdf

This book compiles key protocols instrumental to the study of high-throughput protein production and purification which have been refined and simplified over the years and are now ready to be transferred to any laboratory. Beginning with a section covering general procedures for high-throughput protein production, the volume continues with high-throughput protocols adapted to the production of specific protein families, as well as an extensive section on protocols combining high-throughput protein production and their micro-characterization. Written for the highly successful Methods in Molecular Biology series, chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, High-Throughput Protein Production and Purification: Methods and Protocols serves biochemists ranging from engineers, PhD students and post-doctoral fellows, to the heads of protein expression facilities and researchers, in pursuing this vital area of study.

Author : Joseph A. Marsh
Publisher : Humana
Page : 506 pages
File Size : 49,8 Mb
Release : 2019-04-15
Category : Science
ISBN : 1493992775

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Protein Complex Assembly by Joseph A. Marsh Pdf

This volume details the importance of multiple experimental techniques and computational methods needed to obtain the comprehensive picture of protein complex structure, dynamics and assembly afforded by the emerging field of integrative structural biology. Chapters guide readers through the broad spectrum of approaches required for a complete representation of protein complexes, including expression and purification, experimental characterization of structure and assembly, and computational methods for identifying protein complexes and modelling their assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Complex Assembly: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Author : Anonim
Publisher : Academic Press
Page : 232 pages
File Size : 49,8 Mb
Release : 2018-09-19
Category : Science
ISBN : 9780128150801

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Crop Protection by Anonim Pdf

Crop Protection, Volume 55, the latest release in the Advances in Insect Physiology series, highlights new advances in the field, with this new volume presenting interesting chapters on RNAi Plus, where microbes enhance RNAi: Mechanism and Applications, Symbionts in whiteflies, miRNAs from the microbiome as drivers in the insect, a critical view on insect microbiome data analysis and interpretation, Insect-microbe interactions and transmission as shaped by future climate changes, Intestinal bacteria of the German cockroach and its interaction with entomopathogenic fungus, and Beyond Baculovirus: Alternative biotechnological platforms and pest control based on insect viruses. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Advances in Insect Physiology series Updated release includes the latest information on crop protection

Author : Elena García-Fruitós
Publisher : Humana
Page : 0 pages
File Size : 42,7 Mb
Release : 2016-08-23
Category : Science
ISBN : 1493946757

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Insoluble Proteins by Elena García-Fruitós Pdf

With insolubility proving to be one of the most crippling bottlenecks in the protein production and purification process, this volume serves to aid researchers working in the recombinant protein production field by describing a wide number of protocols and examples. Insoluble Proteins: Methods and Protocols includes chapters that describe not only the recombinant protein production in different expression systems but also different purification and characterization methods to finally obtain these difficult-to-obtain proteins. Beginning with protein production methods using both prokaryotic and eukaryotic expression systems, the book continues with purification protocols using insoluble proteins, the characterization of insoluble proteins, as well as a general overview of interesting applications of insoluble proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, Insoluble Proteins: Methods and Protocols aims to provide the scientific community with detailed and reliable state-of-the-art protocols that are used in order to successfully produce and purify recombinant proteins prone to aggregate.

Author : Ângela Sousa,Luis Passarinha
Publisher : Springer Nature
Page : 462 pages
File Size : 55,8 Mb
Release : 2023-04-24
Category : Science
ISBN : 9781071631478

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Advanced Methods in Structural Biology by Ângela Sousa,Luis Passarinha Pdf

This volume explores the latest integrated bioprocesses and technologies used to study the production of the target recombinant protein of therapeutic or diagnostic interest; its isolation, purification, and stabilization; and the bio-interaction and structural analyses. The chapters in this book are organized into four parts. Part One covers production methods of soluble and membrane proteins in prokaryotic and eukaryotic expression systems, such as Lactococcus lactis and Escherichia coli. Part Two describes traditional and novel approaches for recombinant protein purification and stabilization, and buffers and additives. Part Three discusses automated methods in structural biology based on in silico approaches; and Part Four provides examples of advanced protein investigation methodologies to assess structural analysis such as high-throughput protein crystallization and time-resolved serial crystallography. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Advanced Methods in Structural Biology is a valuable resource to those in academia (i.e., graduate students and postdoctoral researchers) and researchers in the pharmaceutical industry who wish to learn more about this developing field. Chapter 5 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Author : Vijai Kumar Gupta
Publisher : Elsevier
Page : 315 pages
File Size : 41,8 Mb
Release : 2016-10-27
Category : Science
ISBN : 9780444635136

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New and Future Developments in Microbial Biotechnology and Bioengineering by Vijai Kumar Gupta Pdf

New and Future Developments in Microbial Biotechnology and Bioengineering: Aspergillus System Properties and Applications provides information on emerging issues related to recent advancements in aspergillus research and its applications in bioprocess technology, chemical engineering, genome biology, molecular taxonomy, secondary and metabolite production, industrial process and biofuels/bioenergy research, and alternative fuel development. The book covers the various novel enzymes secreted by these fungi and their specific use in the food, textile, pulp and paper, biocellulosic ethanol production, and other industries. The book describes research and experimentation on aspergillus activity and directly connects them to their use in bioprocess technology, chemical engineering, bioremediation process, secondary metabolite production, pharmaceutical processes, protein production, industrial process, biofuels/bioenergy research, and alternative fuel development. Readers will find this book to be an indispensable resource for biotechnologists, biochemical engineers, biochemists, microbiologists, bioinformatics researchers, and other biologists who are interested in learning about the potential applications of these fungi. Compiles available, up-to-date information on recent developments made in the study of aspergillus system properties Contains global content from pioneering international authors Presents current research efforts and links them to various applications, including uses in foods, textiles, pulp and paper, and in biocellulosic ethanol production Provides an indispensable resource for biologists who are interested in learning about the potential applications of the fungi aspergillus

Author : Arnaud Poterszman
Publisher : Humana
Page : 340 pages
File Size : 40,7 Mb
Release : 2021-01-28
Category : Science
ISBN : 1071611259

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Multiprotein Complexes by Arnaud Poterszman Pdf

This volume explores strategies and detailed protocols for the preparation of macromolecular complexes and their characterization in view of structural analysis. The chapters in this book are separated into three parts: Part One focuses on sample preparation, and covers strategies for recombinant expression of multiprotein complexes in prokaryotic and eukaryotic hosts, for genome engineering using the CRISPR/Cas9 system and for production of specific binders such as reformatted antibodies and artificial binding proteins. Part Two looks at the biophysical methods that can provide useful indicators for sample optimization, and often complement structural information obtained with core technologies for structure determination—x-ray crystallography and cryo-electron microscopy—by quantitative solution data. Part Three discusses the characterization of multiprotein complexes in a cellular environment using the latest technologies and in vivo approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Multiprotein Complexes: Methods and Protocols is a valuable resource for structural and molecular biologists who need to prepare multi-components for their applications, and for other scientists working on macromolecular assemblies from other angles that need to know the latest approaches that the field has to offer.

Author : Anonim
Publisher : Unknown
Page : 320 pages
File Size : 53,7 Mb
Release : 2005
Category : Genomics
ISBN : STANFORD:36105123859816

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DOE Genomics by Anonim Pdf

Author : Yaeta Endo,Kazuyuki Takai,Takuya Ueda
Publisher : Humana
Page : 261 pages
File Size : 42,9 Mb
Release : 2016-08-23
Category : Science
ISBN : 1493956981

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Cell-Free Protein Production by Yaeta Endo,Kazuyuki Takai,Takuya Ueda Pdf

During the past decade as the data on gene sequences and expression patterns rapidly accumulated, cell-free protein synthesis technology has also experienced a revolution, becoming a powerful tool for the preparation of proteins for their functional and structural analysis. In Cell-Free Protein Production: Methods and Protocols, experts in the field contribute detailed techniques, the uses of which expand deep into the studies of biochemistry, molecular biology, and biotechnology. Beginning briefly with basic methods and historical aspects, the book continues with thorough coverage of protein preparation methods, the preparation of proteins that are generally difficult to prepare in their functional forms, applications of the cell-free technologies to protein engineering, as well as some methods that are expected to constitute a part of future technologies. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Cell-Free Protein Production: Methods and Protocols aims to help researchers continue the growth of the vital exploration of cell-free sciences and technologies in order to better understand the dynamic lives of cells.

Author : Hyun Ho Park,Qian Yin,Jun Hyuck Lee
Publisher : Frontiers Media SA
Page : 212 pages
File Size : 55,6 Mb
Release : 2023-10-31
Category : Medical
ISBN : 9782832535189

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Structural Immunology of Molecular Innate Immunity by Hyun Ho Park,Qian Yin,Jun Hyuck Lee Pdf

Innate immunity has a critical role in the early stage of the host defense response by the timely recognition of pathogenic or danger signals by various cell surface or cytoplasmic receptors. This is followed by signal transduction via various adaptor and effector molecules. The main functions of innate immunity are to identify and remove the invading pathogens, to recruit various immune cells to the site of infection, and to prepare the adaptive immune response. The Innate immune system has been intensively studied for several decades and now we can begin to understand this system on a molecular level.

Author : Varsha Gupta,Manjistha Sengupta,Jaya Prakash,Baishnab Charan Tripathy
Publisher : Springer
Page : 527 pages
File Size : 42,5 Mb
Release : 2016-10-22
Category : Technology & Engineering
ISBN : 9789811008757

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Basic and Applied Aspects of Biotechnology by Varsha Gupta,Manjistha Sengupta,Jaya Prakash,Baishnab Charan Tripathy Pdf

This book explores the journey of biotechnology, searching for new avenues and noting the impressive accomplishments to date. It has harmonious blend of facts, applications and new ideas. Fast-paced biotechnologies are broadly applied and are being continuously explored in areas like the environmental, industrial, agricultural and medical sciences. The sequencing of the human genome has opened new therapeutic opportunities and enriched the field of medical biotechnology while analysis of biomolecules using proteomics and microarray technologies along with the simultaneous discovery and development of new modes of detection are paving the way for ever-faster and more reliable diagnostic methods. Life-saving bio-pharmaceuticals are being churned out at an amazing rate, and the unraveling of biological processes has facilitated drug designing and discovery processes. Advances in regenerative medical technologies (stem cell therapy, tissue engineering, and gene therapy) look extremely promising, transcending the limitations of all existing fields and opening new dimensions for characterizing and combating diseases.