Amino Acid Analysis Protocols

Author : Catherine Cooper
Publisher : Springer Science & Business Media
Page : 268 pages
File Size : 54,5 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592590476

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Amino Acid Analysis Protocols by Catherine Cooper Pdf

A collection of classic and cutting-edge techniques of high utility in answering specific biological questions about amino acids. Common methods include those based on HPLC or gas chromatography separation and analysis after precolumn derivatization. New techniques based on capillary electrophoresis separation, high-performance anion exchange chromatography, and mass spectrometry are also presented. Each method is described in step-by-step detail to ensure successful experimental results and emphasizes sample preparation, particularly the collection and storage of bodily fluids. Up-to-date and highly practical, Amino Acid Analysis Protocols offers analytical and clinical chemists, as well as a broad range of biological and biomedical investigators, a rich compendium of laboratory tools for the productive analysis of both common and uncommon amino acids.

Author : Michail A. Alterman
Publisher : Unknown
Page : 363 pages
File Size : 50,5 Mb
Release : 2012
Category : Electronic
ISBN : 1617794457

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Amino Acid Analysis by Michail A. Alterman Pdf

Author : Michail A. Alterman,Peter Hunziker
Publisher : Humana Press
Page : 363 pages
File Size : 52,5 Mb
Release : 2011-12-02
Category : Science
ISBN : 1617794449

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Amino Acid Analysis by Michail A. Alterman,Peter Hunziker Pdf

Amino Acid Analysis (AAA) is an integral part of analytical biochemistry. In a relatively short time, the variety of AAA methods has evolved dramatically with more methods shifting to the use of mass spectrometry (MS) as a detection method. Another new aspect is miniaturization. However, most importantly, AAA in this day and age should be viewed in the context of Metabolomics as a part of Systems Biology. Amino Acid Analysis: Methods and Protocols presents a broad spectrum of all available methods allowing for readers to choose the method that most suits their particular laboratory set-up and analytical needs. In this volume, a reader can find chapters describing general as well as specific approaches to the sample preparation. A number of chapters describe specific applications of AAA in clinical chemistry as well as in food analysis, microbiology, marine biology, drug metabolism, even archeology. Separate chapters are devoted to the application of AAA for protein quantitation and chiral AAA. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Amino Acid Analysis: Methods and Protocols provides crucial techniques that can be applied across multiple disciplines by anyone involved in biomedical research or life sciences.

Author : John M. Walker
Publisher : Unknown
Page : 128 pages
File Size : 47,6 Mb
Release : 1984
Category : Molecular biology -- v.236. Plant functional genomics -- v.237. G protein signaling -- v.238. Biopolymer methods in tissue engineering -- v.239. Cell migration in inflammation and immunity -- v.240. Mammalian artificial chromosomes -- v.241. Cell cycle checkpoint control protocols -- v.242. Atomic force microscopy -- v.243. Chiral separations: Methods and protocols -- v.244. Protein purification protocols -- v.245-6. Gene delivery to mammalian cells -- v.247. Drosophila cytogenetics protocols -- v.248. Antibody engineering -- v.249. Cytokine protocols -- v.251. HPLC of peptides and proteins: Methods and protocols -- v.265. RNA interference, editing, and modification -- v.274. Photosynthesis research protocols -- v.318. Plant cell culture protocols -- v.323. Arabidopsis protocols
ISBN : LCCN:84015696

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Methods in Molecular Biology: Amino acid analysis protocols by John M. Walker Pdf

Author : Ibolya Molnár-Perl
Publisher : Elsevier
Page : 669 pages
File Size : 40,6 Mb
Release : 2005-06-27
Category : Science
ISBN : 9780080458458

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Quantitation of Amino Acids and Amines by Chromatography by Ibolya Molnár-Perl Pdf

Quantitation of Amino Acids and Amines by Chromatography: Methods and Protocols is intended to serve as a ready-to-use guide for the identification and quantification of amino acids and amines in various matrices, providing an overview on the theory and protocol of available methods. It presents chromatograms with exact elution programs enabling visual analysis and compares the advantages-disadvantages of various chromatographic techniques. In accordance with the chronological order of the development of chromatographic methods, different techniques are discussed: The possibilities of gas chromatography (GC), followed by those of the high performance liquid chromatography (HPLC) and the most recent techniques capillary electrophoresis (CE), capillary, electrochromatography (CEC). The characteristics of the given chromatographic procedure, relating to the topic in question, are classified according to the preliminary preparation/derivatization process(es), which means the simple methods, suitable for the analysis of the selected compound(s) in natural form, are followed by various derivatization proposals. Detailed protocols provide the reader with guidance in beginning tasks and on how to improve current methods. This book appeals to a wide audience and is recommended for those looking towards the wider reaches of identification and quantification of amino acids and amines. * Provides a systematic, and comprehensive summary of chromatographic techniques and derivatization processes * Compares advantages/disadvantages of various chromatographic techniques * Readers can undertake practical tasks using detailed protocols given in the book

Author : Marie-Isabel Aguilar
Publisher : Springer Science & Business Media
Page : 411 pages
File Size : 44,6 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592597420

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HPLC of Peptides and Proteins by Marie-Isabel Aguilar Pdf

The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.

Author : J. M. Rattenbury
Publisher : Ellis Horwood
Page : 392 pages
File Size : 47,7 Mb
Release : 1981
Category : Medical
ISBN : UOM:39015072635710

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Amino Acid Analysis by J. M. Rattenbury Pdf

Author : John M. Walker
Publisher : Springer Science & Business Media
Page : 759 pages
File Size : 48,6 Mb
Release : 2008-02-12
Category : Science
ISBN : 9781603272599

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The Protein Protocols Handbook by John M. Walker Pdf

In The Protein Protocols Handbook, I have attempted to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benehtop manual and guide both for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. We each, of course, have our own favorite, commonly used gel system, g- staining method, blotting method, and so on; I'm sure you will find yours here. H- ever, I have also described a variety of altematives for many of these techniques; though they may not be superior to the methods you commonly use, they may nev- theless be more appropriate in a particular situation. Only by knowing the range of techniques that are available to you, and the strengths and limitations of these te- niques, will you be able to choose the method that best suits your purpose.

Author : Bryan John Smith
Publisher : Springer Science & Business Media
Page : 489 pages
File Size : 44,9 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592593422

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Protein Sequencing Protocols by Bryan John Smith Pdf

Determination of the protein sequence is as important today as it was a half century ago, even though the techniques and purposes have changed over time. Mass spectrometry has continued its recent rapid development to find notable application in the characterization of small amounts of protein, for example, in the field of proteomics. The “traditional” chemical N-terminal sequencing is still of great value in quality assurance of the increasing number of biopharmaceuticals that are to be found in the clinic, checking processing events of recombinant proteins, and so on. It is joined in the armory of me- ods of protein analysis by such techniques as C-terminal sequencing and amino acid analysis. These methods are continually developing. The first edition of Protein Sequencing Protocols was a “snapshot” of methods in use in protein biochemistry laboratories at the time, and this, the second edition, is likewise. Methods have evolved in the intervening period, and the content of this book has similarly changed, the content of some chapters having been superceded and replaced by other approaches. Thus, in this edition, there is inclusion of approaches to validation of methods for quality assurance work, reflecting the current importance of biopharmaceuticals, and also a guide to further analysis of protein sequence information, acknowledging the importance of bioinformatics.

Author : Robert A. Copeland
Publisher : Springer Science & Business Media
Page : 238 pages
File Size : 54,7 Mb
Release : 2013-11-11
Category : Science
ISBN : 9781475715057

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Methods for Protein Analysis by Robert A. Copeland Pdf

As protein science continues to become an increasingly important aspect of academic and commercial sciences and technology, the need has arisen for a ready source of laboratory protocols for the analysis and evaluation of these biological polymers. Methods for Protein Analysis presents the methods most relevant to the generalist bench scientist working with proteins. A concise yet thorough summary, it covers laboratory methods that can be reasonably performed in a standard protein laboratory, without specialized equipment or expertise. Taking a how to approach, this book examines the techniques used to answer common protein analytical questions and describes methods useful in daily laboratory work. Methods for Protein Analysis is the ideal reference for protein laboratories in academic, government and industrial settings. It is an essential benchtop manual for first-year graduate students beginning their laboratory experience as well as for chemists, biochemists, and molecular biologists in the pharmaceutical, biotechnological, food and specialty chemical industries, and for analysts concerned with the purity and structural integrity of protein. Featuring illustrations and a convenient spiral binding, this guide offers a glossary of common abbreviations and a list of suppliers for protein science.

Author : Paul T. Matsudaira
Publisher : Elsevier
Page : 184 pages
File Size : 44,7 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780080924618

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A Practical Guide to Protein and Peptide Purification for Microsequencing by Paul T. Matsudaira Pdf

Why a Second Edition? The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry. Mass spectrophotometric characterization of proteins and peptides N-terminal sequencing of proteins with blocked N termini Internal amino acid sequence analysis after protease digestion in-gel and on-blot Improved microscale peptide purification methods Computer analysis of protein sequences New protocols tested and refined through everyday use in authors' laboratories Updated reference chapter covering all aspects of protein microsequencing

Author : C. Fini
Publisher : CRC Press
Page : 276 pages
File Size : 50,5 Mb
Release : 2019-08-08
Category : Medical
ISBN : 9781351082372

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Laboratory Methodology in Biochemistry by C. Fini Pdf

Biochemistry Physical Biochemistry Molecular Biology Clinical Biochemistry Biochemical Technology Applied Biochemistry Cellular Biology

Author : Edward A. Lemke
Publisher : Unknown
Page : 411 pages
File Size : 55,8 Mb
Release : 2018
Category : Amino acids
ISBN : 1493975749

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Noncanonical Amino Acids by Edward A. Lemke Pdf

Author : Stanley Blackburn
Publisher : Unknown
Page : 396 pages
File Size : 43,5 Mb
Release : 1978
Category : Science
ISBN : UOM:39015000260516

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Amino Acid Determination by Stanley Blackburn Pdf

Author : Loredano Pollegioni,Stefano Servi
Publisher : Humana Press
Page : 409 pages
File Size : 40,9 Mb
Release : 2011-10-05
Category : Science
ISBN : 1617793302

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Unnatural Amino Acids by Loredano Pollegioni,Stefano Servi Pdf

Even though they are present in nature, non-proteinogenic amino acids are usually defined as unnatural or non-natural. Beside their structural diversity, interest in these compounds is due to their occurrence in nature, their biological properties, the analytical aspects, their use as probes, and their incorporation into peptides and proteins, among other reasons. Divided into five convenient sections, Unnatural Amino Acids: Methods and Protocols deals with enzymatic methods used to produce non-natural amino acids, aspects concerning the presence of unnatural amino acids in peptides with antimicrobial properties, genetic incorporation of unnatural amino acids into proteins (yeast and mammalian cells), and detection and quantification of D-amino acids and related enzymes. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Unnatural Amino Acids: Methods and Protocols serves as an ideal guide for scientists and contributes to directing the attention of researchers to the many fields of growing scientific interest in non-natural amino acids.