Biological Specimen Preparation For Transmission Electron Microscopy

Author : Audrey M. Glauert,Peter R. Lewis
Publisher : Princeton University Press
Page : 349 pages
File Size : 49,7 Mb
Release : 2014-07-14
Category : Science
ISBN : 9781400865024

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Biological Specimen Preparation for Transmission Electron Microscopy by Audrey M. Glauert,Peter R. Lewis Pdf

This book contains all the necessary information and advice for anyone wishing to obtain electron micrographs showing the most accurate ultrastructural detail in thin sections of any type of biological specimen. The guidelines for the choice of preparative methods are based on an extensive survey of current laboratory practice. For the first time, in a textbook of this kind, the molecular events occurring during fixation and embedding are analysed in detail. The reasons for choosing particular specimen preparation methods are explained and guidance is given on how to modify established techniques to suit individual requirements. All the practical methods advocated are clearly described, with accompanying tables and the results obtainable are illustrated with many electron micrographs. Portland Press Series: Practical Methods in Electron Microscopy, Volume 17, Audrey M. Glauert, Editor Originally published in 1999. The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These editions preserve the original texts of these important books while presenting them in durable paperback and hardcover editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.

Author : Jeanne Ayache,Luc Beaunier,Jacqueline Boumendil,Gabrielle Ehret,Danièle Laub
Publisher : Springer Science & Business Media
Page : 250 pages
File Size : 53,7 Mb
Release : 2010-07-03
Category : Technology & Engineering
ISBN : 0387981829

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Sample Preparation Handbook for Transmission Electron Microscopy by Jeanne Ayache,Luc Beaunier,Jacqueline Boumendil,Gabrielle Ehret,Danièle Laub Pdf

Successful transmission electron microscopy in all of its manifestations depends on the quality of the specimens examined. Biological specimen preparation protocols have usually been more rigorous and time consuming than those in the physical sciences. For this reason, there has been a wealth of scienti?c literature detailing speci?c preparation steps and numerous excellent books on the preparation of b- logical thin specimens. This does not mean to imply that physical science specimen preparation is trivial. For the most part, most physical science thin specimen pre- ration protocols can be executed in a matter of a few hours using straightforward steps. Over the years, there has been a steady stream of papers written on various aspects of preparing thin specimens from bulk materials. However, aside from s- eral seminal textbooks and a series of book compilations produced by the Material Research Society in the 1990s, no recent comprehensive books on thin spe- men preparation have appeared until this present work, ?rst in French and now in English. Everyone knows that the data needed to solve a problem quickly are more imp- tant than ever. A modern TEM laboratory with supporting SEMs, light microscopes, analytical spectrometers, computers, and specimen preparation equipment is an investment of several million US dollars. Fifty years ago, electropolishing, chemical polishing, and replication methods were the principal specimen preparation me- ods.

Author : Audrey M. Glauert,Peter R. Lewis
Publisher : Unknown
Page : 0 pages
File Size : 46,9 Mb
Release : 2014-07
Category : Mounting of microscope specimens
ISBN : 0691600155

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Biological Specimen Preparation for Transmission Electron Microscopy by Audrey M. Glauert,Peter R. Lewis Pdf

This book contains all the necessary information and advice for anyone wishing to obtain electron micrographs showing the most accurate ultrastructural detail in thin sections of any type of biological specimen. The guidelines for the choice of preparative methods are based on an extensive survey of current laboratory practice. For the first time, in a textbook of this kind, the molecular events occurring during fixation and embedding are analysed in detail. The reasons for choosing particular specimen preparation methods are explained and guidance is given on how to modify established techniques to suit individual requirements. All the practical methods advocated are clearly described, with accompanying tables and the results obtainable are illustrated with many electron micrographs. Portland Press Series: Practical Methods in Electron Microscopy, Volume 17, Audrey M. Glauert, Editor Originally published in 1999. The Princeton Legacy Library uses the latest print-on-demand technology to again make available previously out-of-print books from the distinguished backlist of Princeton University Press. These editions preserve the original texts of these important books while presenting them in durable paperback and hardcover editions. The goal of the Princeton Legacy Library is to vastly increase access to the rich scholarly heritage found in the thousands of books published by Princeton University Press since its founding in 1905.

Author : Jeanne Ayache,Luc Beaunier,Jacqueline Boumendil,Gabrielle Ehret,Danièle Laub
Publisher : Springer Science & Business Media
Page : 338 pages
File Size : 47,8 Mb
Release : 2010-06-08
Category : Technology & Engineering
ISBN : 1441959742

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Sample Preparation Handbook for Transmission Electron Microscopy by Jeanne Ayache,Luc Beaunier,Jacqueline Boumendil,Gabrielle Ehret,Danièle Laub Pdf

Successful transmission electron microscopy in all of its manifestations depends on the quality of the specimens examined. Biological specimen preparation protocols have usually been more rigorous and time consuming than those in the physical sciences. For this reason, there has been a wealth of scienti c literature detailing speci c preparation steps and numerous excellent books on the preparation of b- logical thin specimens. This does not mean to imply that physical science specimen preparation is trivial. For the most part, most physical science thin specimen pre- ration protocols can be executed in a matter of a few hours using straightforward steps. Over the years, there has been a steady stream of papers written on various aspects of preparing thin specimens from bulk materials. However, aside from s- eral seminal textbooks and a series of book compilations produced by the Material Research Society in the 1990s, no recent comprehensive books on thin specimen preparation have appeared until this present work, rst in French and now in English. Everyone knows that the data needed to solve a problem quickly are more imp- tant than ever. A modern TEM laboratory with supporting SEMs, light microscopes, analytical spectrometers, computers, and specimen preparation equipment is an investment of several million US dollars. Fifty years ago, electropolishing, chemical polishing, and replication methods were the principal specimen preparation me- ods.

Author : PJ Goodhew
Publisher : Garland Science
Page : 50 pages
File Size : 47,7 Mb
Release : 2020-11-25
Category : Science
ISBN : 9781000142761

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Specimen Preparation for Transmission Electron Microscopy of Materials by PJ Goodhew Pdf

Details the essential practical steps which must precede microscopy. Methods for preparing sheet or disc specimens and final thinning techniques are described with reference to practical problems. The book also covers methods for mounting specimens in the

Author : Michael J. Dykstra
Publisher : Springer Science & Business Media
Page : 368 pages
File Size : 42,5 Mb
Release : 2012-12-06
Category : Science
ISBN : 9781468400106

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Biological Electron Microscopy by Michael J. Dykstra Pdf

In this practical text, the author covers the fundamentals of biological electron microscopy - including fixation, instrumentation, and darkroom work - to provide an excellent introduction to the subject for the advanced undergraduate or graduate student.

Author : Peter J. Goodhew
Publisher : Garland Science
Page : 56 pages
File Size : 54,7 Mb
Release : 1984
Category : Electron microscope, Transmission
ISBN : UCAL:B4564362

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Specimen Preparation for Transmission Electron Microscopy of Materials by Peter J. Goodhew Pdf

Details the essential practical steps which must precede microscopy. Methods for preparing sheet or disc specimens and final thinning techniques are described with reference to practical problems. The book also covers methods for mounting specimens in the

Author : John J. Bozzola,Lonnie Dee Russell
Publisher : Jones & Bartlett Learning
Page : 702 pages
File Size : 46,6 Mb
Release : 1999
Category : Medical
ISBN : 0763701920

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Electron Microscopy by John J. Bozzola,Lonnie Dee Russell Pdf

New edition of an introductory reference that covers all of the important aspects of electron microscopy from a biological perspective, including theory of scanning and transmission; specimen preparation; darkroom, digital imaging, and image analysis; laboratory safety; interpretation of images; and an atlas of ultrastructure. Generously illustrated with bandw line drawings and photographs. Annotation copyrighted by Book News, Inc., Portland, OR

Author : James Pawley,Heide Schatten
Publisher : Springer Science & Business Media
Page : 323 pages
File Size : 52,7 Mb
Release : 2007-12-03
Category : Science
ISBN : 9780387729725

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Biological Low-Voltage Scanning Electron Microscopy by James Pawley,Heide Schatten Pdf

Major improvements in instrumentation and specimen preparation have brought SEM to the fore as a biological imaging technique. Although this imaging technique has undergone tremendous developments, it is still poorly represented in the literature, limited to journal articles and chapters in books. This comprehensive volume is dedicated to the theory and practical applications of FESEM in biological samples. It provides a comprehensive explanation of instrumentation, applications, and protocols, and is intended to teach the reader how to operate such microscopes to obtain the best quality images.

Author : M. A. Nasser Hajibagheri
Publisher : Springer Science & Business Media
Page : 292 pages
File Size : 45,6 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592592012

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Electron Microscopy Methods and Protocols by M. A. Nasser Hajibagheri Pdf

Electron Microscopy Methods and Protocols is designed for the established researcher as a manual for extending knowledge of the field. It is also for the newcomer who wishes to move into the field. A wide range of applications for the examination of cells, tissues, biological macromolecules, molecular structures, and their interactions are discussed. We have tried to gather together methods that we consider to be those most generally appli- ble to current research in both cell and molecular biology. Each chapter c- tains a set of related practical protocols with examples provided by experts who have first-hand knowledge of the techniques they describe. The individual chapters are grouped according to similarities in their specimen preparation and methodology. Methods are presented in detail, in a step-by-step fashion, using reproducible protocols the authors have personally checked. During the last decade, the scientific literature describing the use of colloidal gold as an immunocytochemical marker has increased at an ex- nential rate, and this trend is expected to continue. We have included a large number of variations on the immunogold labeling technique. In both the ne- tive staining and cryo chapters, authors emphasize the “immunological app- cations” in order to correlate as fully as possible with the emphasis on immunogold labeling in the other chapters. Electron Microscopy Methods and Protocols commences with the routine preparation of biological material for classical transmission electron microscopy involving tissue fixation, embedding, and sectioning (Chap. 1).

Author : Patrick Echlin
Publisher : Springer Science & Business Media
Page : 329 pages
File Size : 55,8 Mb
Release : 2011-04-14
Category : Technology & Engineering
ISBN : 9780387857312

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Handbook of Sample Preparation for Scanning Electron Microscopy and X-Ray Microanalysis by Patrick Echlin Pdf

Scanning electr on microscopy (SEM) and x-ray microanalysis can produce magnified images and in situ chemical information from virtually any type of specimen. The two instruments generally operate in a high vacuum and a very dry environment in order to produce the high energy beam of electrons needed for imaging and analysis. With a few notable exceptions, most specimens destined for study in the SEM are poor conductors and composed of beam sensitive light elements containing variable amounts of water. In the SEM, the imaging system depends on the specimen being sufficiently electrically conductive to ensure that the bulk of the incoming electrons go to ground. The formation of the image depends on collecting the different signals that are scattered as a consequence of the high energy beam interacting with the sample. Backscattered electrons and secondary electrons are generated within the primary beam-sample interactive volume and are the two principal signals used to form images. The backscattered electron coefficient ( ? ) increases with increasing atomic number of the specimen, whereas the secondary electron coefficient ( ? ) is relatively insensitive to atomic number. This fundamental diff- ence in the two signals can have an important effect on the way samples may need to be prepared. The analytical system depends on collecting the x-ray photons that are generated within the sample as a consequence of interaction with the same high energy beam of primary electrons used to produce images.

Author : Lars Kaestner,Peter Lipp
Publisher : Springer
Page : 132 pages
File Size : 45,5 Mb
Release : 2018-12-07
Category : Science
ISBN : 9783319953045

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Microscopy of the Heart by Lars Kaestner,Peter Lipp Pdf

This book provides in depths information on different microscopy approaches and supplies the reader with methods how to untangle highly complex processes involved in physiological and pathophysiological cardiac signaling. Microscopy approaches have established themselves as the quasi gold standard that enables us to appreciate the underlying mechanisms of physiological and pathophysiological cardiac signaling. This book presents the most important microscopy techniques from the level of individual molecule e.g. Förster-Resonance Energy Transfer (FRET), up to cellular and tissue imaging, e.g. electron microscopy (TEM) or light sheet microscopy. The book is intended for graduate students and postdocs in cardiovascular research, imaging and cell biology, pre-clinical and clinical researchers in cardiovascular sciences as well as decision makers of the pharmaceutical industry.

Author : Judith A. Murphy,Godfried M. Roomans
Publisher : Unknown
Page : 364 pages
File Size : 53,5 Mb
Release : 1984
Category : Science
ISBN : UOM:39015010050725

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Preparation of Biological Specimens for Scanning Electron Microscopy by Judith A. Murphy,Godfried M. Roomans Pdf

Author : Michael J. Dykstra
Publisher : Springer Science & Business Media
Page : 280 pages
File Size : 42,9 Mb
Release : 1993-08-31
Category : Medical
ISBN : 0306444496

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A Manual of Applied Techniques for Biological Electron Microscopy by Michael J. Dykstra Pdf

This easy-to-follow manual describes tested procedures used to prepare biological samples for scanning and transmission electron microscopy, as well as methods for cytochemistry, immunocytochemistry, and scientific photography. The work is structured to clearly define testing objectives, necessary materials, procedural steps, and expected results; a list of references and trouble shooting techniques round out the text.

Author : R. L. Griffin
Publisher : Prentice Hall
Page : 122 pages
File Size : 55,8 Mb
Release : 1990
Category : Science
ISBN : UOM:39015019424780

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Using the Transmission Electron Microscope in the Biological Sciences by R. L. Griffin Pdf