Biomedical Electron Microscopy

Author : Arvid B. Maunsbach,Björn A. Afzelius
Publisher : Elsevier
Page : 548 pages
File Size : 43,5 Mb
Release : 1998-11-03
Category : Science
ISBN : 0080528090

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Biomedical Electron Microscopy by Arvid B. Maunsbach,Björn A. Afzelius Pdf

This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis. Authored by the key leaders in the biological electron microscopy field Illustrates both optimal and suboptimal or artifactual results in a variety of electron microscopy disciplines Introduces students on how to read and interpret electron micrographs

Author : Pier Carlo Braga,Davide Ricci
Publisher : Springer Science & Business Media
Page : 388 pages
File Size : 43,9 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592596478

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Atomic Force Microscopy by Pier Carlo Braga,Davide Ricci Pdf

The natural, biological, medical, and related sciences would not be what they are today without the microscope. After the introduction of the optical microscope, a second breakthrough in morphostructural surface analysis occurred in the 1940s with the development of the scanning electron microscope (SEM), which, instead of light (i. e. , photons) and glass lenses, uses electrons and electromagnetic lenses (magnetic coils). Optical and scanning (or transmission) electron microscopes are called “far-field microscopes” because of the long distance between the sample and the point at which the image is obtained in comparison with the wavelengths of the photons or electrons involved. In this case, the image is a diffraction pattern and its resolution is wavelength limited. In 1986, a completely new type of microscopy was proposed, which, without the use of lenses, photons, or electrons, directly explores the sample surface by means of mechanical scanning, thus opening up unexpected possibilities for the morphostructural and mechanical analysis of biological specimens. These new scanning probe microscopes are based on the concept of near-field microscopy, which overcomes the problem of the limited diffraction-related resolution inherent in conventional microscopes. Located in the immediate vicinity of the sample itself (usually within a few nanometers), the probe records the intensity, rather than the interference signal, thus significantly improving resolution. Since the most we- known microscopes of this type operate using atomic forces, they are frequently referred to as atomic force microscopes (AFMs).

Author : David G. Robinson,Ulrich Ehlers,Rainer Herken,Bernd Herrmann,Frank Mayer,Friedrich-Wilhelm Schürmann
Publisher : Springer Science & Business Media
Page : 204 pages
File Size : 43,7 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642488481

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Methods of Preparation for Electron Microscopy by David G. Robinson,Ulrich Ehlers,Rainer Herken,Bernd Herrmann,Frank Mayer,Friedrich-Wilhelm Schürmann Pdf

In 1939, when the electron optics laboratory of Siemens & Halske Inc. began to manufacture the first electron microscopes, the biological and medical profes sions had an unexpected instrument at their disposal which exceeded the reso lution of the light microscope by more than a hundredfold. The immediate and broad application of this new tool was complicated by the overwhelming prob lems inherent in specimen preparation for the investigation of cellular struc tures. The microtechniques applied in light microscopy were no longer appli cable, since even the thinnest paraffin layers could not be penetrated by electrons. Many competent biological and medical research workers expressed their anxiety that objects in high vacuum would be modified due to complete dehydration and the absorbed electron energy would eventually cause degrada tion to rudimentary carbon backbones. It also seemed questionable as to whether it would be possible to prepare thin sections of approximately 0. 5 11m from heterogeneous biological specimens. Thus one was suddenly in posses sion of a completely unique instrument which, when compared with the light microscope, allowed a 10-100-fold higher resolution, yet a suitable preparation methodology was lacking. This sceptical attitude towards the application of electron microscopy in bi ology and medicine was supported simultaneously by the general opinion of colloid chemists, who postulated that in the submicroscopic region of living structures no stable building blocks existed which could be revealed with this apparatus.

Author : Gisele M. Hodges,Richard C. Hallowes
Publisher : Unknown
Page : 336 pages
File Size : 52,9 Mb
Release : 1979
Category : Science
ISBN : PSU:000010860373

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Biomedical Research Applications of Scanning Electron Microscopy by Gisele M. Hodges,Richard C. Hallowes Pdf

Author : Katharine E. Carr,Peter G. Toner
Publisher : Unknown
Page : 410 pages
File Size : 46,7 Mb
Release : 1982
Category : Science
ISBN : UOM:39015004434612

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Cell Structure by Katharine E. Carr,Peter G. Toner Pdf

Author : Terje Dokland,Dietmar Werner Hutmacher,Mary Mah-lee Ng,Jan-thorsten Schantz
Publisher : World Scientific Publishing Company
Page : 422 pages
File Size : 40,6 Mb
Release : 2006-09-28
Category : Technology & Engineering
ISBN : 9789813101951

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Techniques In Microscopy For Biomedical Applications by Terje Dokland,Dietmar Werner Hutmacher,Mary Mah-lee Ng,Jan-thorsten Schantz Pdf

The second volume of the series Manuals in Biomedical Research, this book is aimed to be both a concise introduction to the diverse field of microscopy and a practical guide those who require the use of microscopic for methods in their research. It provides young as well as experienced scientists a state-of-the-art multidisciplinary overview of microscopic techniques, covering all the major microscopy fields in biomedical sciences and showing their application in evaluating samples ranging from molecules to cells and tissues.Microscopy has revolutionized our understanding of biological events. Within the last two decades, microscopic techniques have provided insights into the dynamics of biological processes that regulate such events. Biological discovery, to a large extent, depends on advances in imaging techniques and various microscopic techniques have emerged as central and indispensable tools in the biomedical sciences.The four authors bring with them extensive experiences spanning across disciplines such as Microbiology, Molecular and Cell Biology, Tissue Engineering, Biomedical and Regenerative Medicine and so forth, reinforcing the fact that microscopy has proven useful in countless investigations into the mysteries of life.

Author : Gisele M. Hodges
Publisher : Unknown
Page : 128 pages
File Size : 41,6 Mb
Release : 1980
Category : Electronic
ISBN : OCLC:630622933

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Biomedical Research Applications of Scanning Electron Microscopy by Gisele M. Hodges Pdf

Author : Pushpa Viswanathan
Publisher : MJP Publisher
Page : 570 pages
File Size : 41,8 Mb
Release : 2019-06-12
Category : Science
ISBN : 8210379456XXX

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Electron Microscopy by Pushpa Viswanathan Pdf

The advent of electron microscopes has opened up new vistas in the field of science. The ultrastructural morphological evidence offered by electron microscope to substantiate and support other findings is highly rewarding. This book gives a comprehensive overview of the principle and operations of the electron microscope. Numerous electron micrographs have been provided to acquaint the reader with the appearance of highly magnified features seen through the EM. This book would definitely create “a feel for this subject” particularly among those who want to use this technique for their research work.

Author : Saul Wischnitzer
Publisher : Elsevier
Page : 311 pages
File Size : 49,6 Mb
Release : 2013-10-22
Category : Science
ISBN : 9781483148694

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Introduction to Electron Microscopy by Saul Wischnitzer Pdf

Introduction to Electron Microscopy, Second Edition provides an introduction to the foundations of electron microscopy; an outline of some practical aspects of instrument operation; and discussion of the rationale of the methodology of biological specimen preparation. The book seeks to provide a comprehensive understanding of the theoretical and operational aspects of the electron microscope. This edition consists of two parts. Part One deals with the history, basic theory, and operation of the electron microscope. Part Two discusses steps used in material preparation for electron microscope investigation such as fixation, embedding, and staining techniques. Biomedical researchers, molecular biologists, toxicologists, forensic investigators, and medical students will find this book a very useful reference.

Author : Elizabeth M. Slayter,Henry S. Slayter
Publisher : Cambridge University Press
Page : 336 pages
File Size : 40,8 Mb
Release : 1992-10-30
Category : Science
ISBN : 0521339480

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Light and Electron Microscopy by Elizabeth M. Slayter,Henry S. Slayter Pdf

The compound optical microscope, in its various modern forms, is probably the most familiar of all laboratory instruments and the electron microscope, once an exotic rarity, has now become a standard tool in biological and materials research. Both instruments are often used effectively with little knowledge of the relevant theory, or even of how a particular type of microscope functions. Eventually however, proper use, interpretation of images and choices of specific applications demand an understanding of fundamental principles. This book describes the principles of operation of each type of microscope currently available and of use to biomedical and materials scientists. It explains the mechanisms of image formation, contrast and its enhancement, accounts for ultimate limits on the size of observable details (resolving power and resolution) and finally provides an account of Fourier optical theory. Principles behind the photographic methods used in microscopy are also described and there is some discussion of image processing methods. The book will appeal to graduate students and researchers in the biomedical sciences, and it will be helpful to students taking a course involving the principles of microscopy.

Author : Benjamin M. Siegel,Donald Robert Beaman
Publisher : Unknown
Page : 496 pages
File Size : 52,8 Mb
Release : 1975
Category : Science
ISBN : STANFORD:36105031608982

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Physical Aspects of Electron Microscopy and Microbeam Analysis by Benjamin M. Siegel,Donald Robert Beaman Pdf

Transmission electron microscopy; Resolution and contrast; Physical applications (Materials and metallurgical applications) using high voltage, conventional, and scanning microscopy; Biophysical: radiation damage; Energy analysis; Instrumentation: field emission illuminaling Systems.

Author : Peter Ingram,John D. Shelburne,Victor L. Roggli,Ann LeFurgey
Publisher : Academic Press
Page : 545 pages
File Size : 52,7 Mb
Release : 1999-10-29
Category : Science
ISBN : 0080524567

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Biomedical Applications of Microprobe Analysis by Peter Ingram,John D. Shelburne,Victor L. Roggli,Ann LeFurgey Pdf

Biomedical Applications of Microprobe Analysis is a combination reference/laboratory manual for the use of microprobe analysis in both clinical diagnostic and research settings. Also called microchemical microscopy, microprobe analysis uses high-energy bombardment of cells and tissue, in combination with high resolution EM or confocal microscopy to provide a profile of the ion, metal, and mineral concentrations present in a sample. This allows insight into the physiology and pathophysiology of a wide variety of cells and tissues. This book describes methods for obtaining detailed information about the identity and composition of particles too small to be seen with the naked eye and describes how this information can be useful in diagnostic and biomedical research. Up-to-date review of electron microprobe analysis Detailed descriptions of sample preparation techniques Recent technologies including confocal microscopy, infrared microspectroscopy, and laser raman spectroscopy Over 100 illustrations with numerous specific applications Contributions by world-renowned experts in the field Brief summary of highlights precedes each chapter

Author : Douglas E. Chandler,Robert W. Roberson
Publisher : Jones & Bartlett Publishers
Page : 468 pages
File Size : 44,6 Mb
Release : 2009
Category : Medical
ISBN : 9780763738747

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Bioimaging by Douglas E. Chandler,Robert W. Roberson Pdf

The Development Of Microscopy Revolutionized The World Of Cell And Molecular Biology As We Once Knew It And Will Continue To Play An Important Role In Future Discoveries. Bioimaging: Current Concepts In Light And Electron Microscopy Is The Optimal Text For Any Undergraduate Or Graduate Bioimaging Course, And Will Serve As An Important Reference Tool For The Research Scientist. This Unique Text Covers, In Great Depth, Both Light And Electron Microscopy, As Well As Other Structure And Imaging Techniques Like X-Ray Crystallography And Atomic Force Microscopy. Written In A User-Friendly Style And Covering A Broad Range Of Topics, Bioimaging Describes The State-Of-The-Art Technologies That Have Powered The Field To The Forefront Of Cellular And Molecular Biological Research.

Author : Joachim Frank
Publisher : Oxford University Press
Page : 432 pages
File Size : 53,7 Mb
Release : 2006-02-02
Category : Science
ISBN : 9780190292881

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Three-Dimensional Electron Microscopy of Macromolecular Assemblies by Joachim Frank Pdf

Cryoelectron microscopy of biological molecules is among the hottest growth areas in biophysics and structural biology at present, and Frank is arguably the most distinguished practitioner of this art. CryoEM is likely over the next few years to take over much of the structural approaches currently requiring X-ray crystallography, because one can now get good and finely detailed images of single molecules down to as little as 200,000 MW, covering a substantial share of the molecules of greatest biomedical research interest. This book, the successor to an earlier work published in 1996 with Academic Press, is a natural companion work to our forthcoming book on electron crystallography by Robert Glaeser, with contributions by six others, including Frank. A growing number of workers will employ CryoEM for structural studies in their own research, and a large proportion of biomedical researchers will have a growing interest in understanding what the capabilities and limits of this approach are.

Author : Ray Egerton
Publisher : Springer Science & Business Media
Page : 224 pages
File Size : 42,9 Mb
Release : 2011-02-11
Category : Technology & Engineering
ISBN : 0387258000

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Physical Principles of Electron Microscopy by Ray Egerton Pdf

Scanning and stationary-beam electron microscopes are indispensable tools for both research and routine evaluation in materials science, the semiconductor industry, nanotechnology and the biological, forensic, and medical sciences. This book introduces current theory and practice of electron microscopy, primarily for undergraduates who need to understand how the principles of physics apply in an area of technology that has contributed greatly to our understanding of life processes and "inner space." Physical Principles of Electron Microscopy will appeal to technologists who use electron microscopes and to graduate students, university teachers and researchers who need a concise reference on the basic principles of microscopy.