Calcium Binding Protein Protocols

Author : Hans J. Vogel
Publisher : Springer Science & Business Media
Page : 346 pages
File Size : 42,8 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781592591831

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Calcium-Binding Protein Protocols by Hans J. Vogel Pdf

Calcium plays an important role in a wide variety of biological processes. This divalent metal ion can bind to a large number of proteins; by doing so it modifies their biological activity or their stability. Because of its distinct che- cal properties calcium is uniquely suited to act as an on–off switch or as a light dimmer of biological activities. The two books entitled Calcium-Binding Protein Protocols (Volumes I and II) focus on modern experimental analyses and methodologies for the study of calcium-binding proteins. Both extracel- lar and intracellular calcium-binding proteins are discussed in detail. H- ever, proteins involved in calcium handling (e. g. , calcium pumps and calcium channels), fall outside of the scope of these two volumes. Also, calcium-bi- ing proteins involved in bone deposition will not be discussed, as this specific topic has been addressed previously. The focus of these two books is on studies of the calcium-binding proteins and their behavior in vitro and in vivo. The primary emphasis is on protein chemistry and biophysical methods. Many of the methods described will also be applicable to proteins that do not bind calcium. Calcium-Binding Protein Protocols is divided into three main sections. The section entitled Introduction and Reviews provides information on the role of calcium in intracellular secondary messenger activation mechanisms. Mo- over, unique aspects of calcium chemistry and the utilization of calcium in dairy proteins, as well as calcium-binding proteins involved in blood clotting, are addressed.

Author : Hans J. Vogel
Publisher : Unknown
Page : 0 pages
File Size : 54,9 Mb
Release : 2002
Category : Calcium-binding proteins
ISBN : OCLC:460032655

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Calcium-binding Protein Protocols by Hans J. Vogel Pdf

This handbook focuses on modern experimental analyses and methodologies for the study of calcium-binding proteins.

Author : Hans J. Vogel
Publisher : Springer Science & Business Media
Page : 415 pages
File Size : 44,7 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592591848

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Calcium-Binding Protein Protocols by Hans J. Vogel Pdf

Calcium plays an important role in a wide variety of biological processes. This divalent metal ion can bind to a large number of proteins; by doing so it modifies their biological activity or their stability. Because of its distinct che- cal properties calcium is uniquely suited to act as an on–off switch or as a light dimmer of biological activities. The two books entitled Calcium-Binding Protein Protocols (Volumes I and II) focus on modern experimental analyses and methodologies for the study of calcium-binding proteins. Both extracel- lar and intracellular calcium-binding proteins are discussed in detail. H- ever, proteins involved in calcium handling (e. g. , calcium pumps and calcium channels), fall outside of the scope of these two volumes. Also, calcium-bi- ing proteins involved in bone deposition will not be discussed, as this specific topic has been addressed previously. The focus of these two books is on studies of the calcium-binding proteins and their behavior in vitro and in vivo. The primary emphasis is on protein chemistry and biophysical methods. Many of the methods described will also be applicable to proteins that do not bind calcium. Calcium-Binding Protein Protocols is divided into three main sections. The section entitled Introduction and Reviews provides information on the role of calcium in intracellular secondary messenger activation mechanisms. Mo- over, unique aspects of calcium chemistry and the utilization of calcium in dairy proteins, as well as calcium-binding proteins involved in blood clotting, are addressed.

Author : Claus W. Heizmann
Publisher : Unknown
Page : 779 pages
File Size : 55,5 Mb
Release : 2019
Category : Biochemistry
ISBN : 1493990306

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Calcium-Binding Proteins of the EF-Hand Superfamily by Claus W. Heizmann Pdf

This detailed volume explores protocols for studying the many facets of Ca2+-imaging, Ca2+-signaling, and Ca2+-binding along with background information on the principles and application of these techniques. The content of the book delves into 48 chapters including subjects such as data analysis and modern technologies to study calcium-binding and signaling in cells, the superfamily of calcium-binding proteins characterized by the EF-hand structural motif, as well as their use as diagnostic and prognostic biomarkers in Laboratory Medicine and novel therapeutic drug targets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, Calcium-Binding Proteins of the EF-Hand Superfamily: From Basics to Medical Applications presents state-of-the-art, lab-based methods and easy-to-follow protocols for daily use, making it interesting for basic and medical researchers, cell- and molecular biologists, clinicians, clinical chemists, and the diagnostic industry.

Author : Claus Heizmann
Publisher : Humana Press
Page : 440 pages
File Size : 41,5 Mb
Release : 2016-05-01
Category : Science
ISBN : 149395976X

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Calcium-Binding Proteins and Rage by Claus Heizmann Pdf

This Methods in Molecular Biology book reviews common techniques for studying calcium binding proteins, underlining the diagnostic and clinical importance of this family of proteins. Offers materials lists, protocols, troubleshooting advice and more."

Author : Eugene Permyakov,Robert H. Kretsinger
Publisher : John Wiley & Sons
Page : 456 pages
File Size : 48,8 Mb
Release : 2011-03-21
Category : Science
ISBN : 9781118099544

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Calcium Binding Proteins by Eugene Permyakov,Robert H. Kretsinger Pdf

Calcium Binding Proteins explains the unique and highly diverse functions of calcium in biology, which are realized by calcium binding proteins. The structures and physical characteristics of these calcium binding proteins are described, as well as their functions and general patterns of their evolution. Techniques that underlie the description of proteins are discussed, including NMR, circular dichroism, optical rotatory dispersion spectroscopy, calorimetry,and crystallography. The book discusses the patterns of bochmical phenomena such as calcium homeostasis, mineralization, and cell signaling that involve specific proteins. It summarizes ongoing research and presents general hypotheses that help to focus future research, and also provides a conceptual framework and a description of the underlying techniques that permits someone entering the field to become conversant.

Author : Charles Gerday,L. Bolis,R. Gilles
Publisher : Springer Science & Business Media
Page : 265 pages
File Size : 55,9 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642730429

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Calcium and Calcium Binding Proteins by Charles Gerday,L. Bolis,R. Gilles Pdf

The idea of Professors Bolis and Gilles to gather together for a 3 days' meeting in the splendid environment of Crans-Montana in Switzerland a limited number of people around the subject of calcium and calcium bind ing proteins seemed at first particularly attractive, and when they asked me to take charge of the scientific organization of the symposium, I accepted with enthusiasm. It rapidly became clear that the major problem would be the selection of the topics, since it was impossible to cover completely and in depth such a broad and dynamic area of research. In our view, one imperative was to associate as intimately as possible the structural and the functional aspects of the areas covered. Apart from one whole day focused on the fascinating roles played by calmodulin in cellular activities, the other sessions were devoted to calmodulin-related calcium binding proteins in muscle and non muscle tissues and to some selected biological systems such as mitochondria, secretory cells or sarcoplasmic reticulum in which calcium also plays a crucial role. The presentations were made by leading investigators in their field. Some of them do not, however, appear in the present volume, for which there are two reasons: first, some of the contributions were somewhat outside the scope of the book; second, three speakers, for valid reasons, simply found no opportunity to write a manuscript in the allotted time.

Author : Anthony Norman
Publisher : Elsevier
Page : 650 pages
File Size : 43,6 Mb
Release : 2012-12-02
Category : Medical
ISBN : 9780323141420

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Calcium-Binding Proteins in Health and Disease by Anthony Norman Pdf

Calcium-Binding Proteins in Health and Disease includes the papers prepared by the invited speakers as well as many of the free communications. The contributions are grouped according to their general subject matter, based on the classification made by the authors, e.g., Extracellular Metabolism: Calcium Homeostasis; Low-Affinity Calcium-Binding Proteins. There can be little doubt that calcium-binding proteins is a field of scientific endeavor which will continue to produce results of great interest to modern biology. This book is divided into eight sections, the first of which, tackle extracellular calcium metabolism or calcium homeostasis. The next sections focus on topics such as low-affinity calcium-binding proteins; calcium and membranes, channels and transport (pumps); the role of calcium in complex metabolic processes; and gene structure of calcium-binding proteins and their expression. Other chapters cover physical studies on calcium-binding proteins, including X-ray, crystallography, and NMR; structure-function relationships of calcium-binding proteins and their targets; and calcium-binding proteins in health and disease. This book will be of interest to practitioners in the fields of biology and medicine.

Author : Marvin P. Thompson
Publisher : C R C Press Reprints
Page : 160 pages
File Size : 55,6 Mb
Release : 1988
Category : Medical
ISBN : UOM:39015012567247

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Calcium-binding Proteins by Marvin P. Thompson Pdf

Author : David G. Lambert
Publisher : Springer Science & Business Media
Page : 351 pages
File Size : 47,5 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592592500

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Calcium Signaling Protocols by David G. Lambert Pdf

2+ The regulation of intracellular Ca is a common theme presented in many 2+ papers over the last 20 or so years, and the description of the Ca -sensitive indicator dye fura 2 in 1985 resulted in a massive increase in these types of 2+ studies. Aspects of the regulation of intracellular Ca have been dealt with in many of the subsequent chapters and will therefore not be covered again. Calcium Signaling Protocols results from a chance discussion with Dr. R. I. Norman of the Department of Medicine at Leicester University and r- resents a major effort from a group of extremely helpful and very patient - thors. Putting a book like this together takes time and I am indebted to these authors without whom this project would have remained a chance discussion. I am also very grateful to Professor J. M. Walker, the series editor, for all his help and advice over the course of this project and particularly his help editing the first batch of chapters. I would also like to thank Dr E. L. Pallett for help and advice regarding interconversion of Mac and Word files and for archiving chapters.

Author : C. W. Heizmann,Ernesto Carafoli
Publisher : Unknown
Page : 102 pages
File Size : 42,6 Mb
Release : 1994
Category : Electronic
ISBN : OCLC:437073583

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Calcium-binding Proteins in Normal and Transformed Cells by C. W. Heizmann,Ernesto Carafoli Pdf

Author : Marvin P. Thompson
Publisher : CRC-Press
Page : 168 pages
File Size : 45,9 Mb
Release : 1988-03-31
Category : Science
ISBN : 0849348587

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Calcium Binding Proteins by Marvin P. Thompson Pdf

Author : Hiroshi Kawasaki,Robert H. Kretsinger
Publisher : Unknown
Page : 175 pages
File Size : 45,6 Mb
Release : 1994
Category : Calcium-binding proteins
ISBN : OCLC:248870590

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Calcium-binding Proteins 1 by Hiroshi Kawasaki,Robert H. Kretsinger Pdf

Author : Benedetto De Bernard
Publisher : Elsevier Science & Technology
Page : 502 pages
File Size : 53,9 Mb
Release : 1983
Category : Calcium in the body
ISBN : UCAL:B4493358

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Calcium-binding Proteins, 1983 by Benedetto De Bernard Pdf

Author : Claus W. Heizmann
Publisher : Springer
Page : 624 pages
File Size : 40,8 Mb
Release : 1991-03-28
Category : Science
ISBN : 3540532773

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Novel Calcium-Binding Proteins by Claus W. Heizmann Pdf

A wide variety of hormones, neurotransmitters and growth factors exert their cellular effects by reacting first with membrane receptors resulting in an increase of intracellular calcium and the cellular response. The calcium signal in the cell is mediated by the high-affinity calcium binding proteins (characterized by the EF-hand structural element), and by the calcium and phospholipid dependent proteins. Many of these have been discovered most recently. Their purification, distribution, protein and gene structures as well as their physiological roles are discussed. The book is of interest to biochemists and molecular biologists as well as to clinicians and the pharmaceutical industry who can apply the results in this field.