Calculations For Molecular Biology And Biotechnology

Author : Frank H. Stephenson
Publisher : Academic Press
Page : 460 pages
File Size : 45,9 Mb
Release : 2010-07-30
Category : Science
ISBN : 012375691X

Get Book

Calculations for Molecular Biology and Biotechnology by Frank H. Stephenson Pdf

Calculations for Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory, Second Edition, provides an introduction to the myriad of laboratory calculations used in molecular biology and biotechnology. The book begins by discussing the use of scientific notation and metric prefixes, which require the use of exponents and an understanding of significant digits. It explains the mathematics involved in making solutions; the characteristics of cell growth; the multiplicity of infection; and the quantification of nucleic acids. It includes chapters that deal with the mathematics involved in the use of radioisotopes in nucleic acid research; the synthesis of oligonucleotides; the polymerase chain reaction (PCR) method; and the development of recombinant DNA technology. Protein quantification and the assessment of protein activity are also discussed, along with the centrifugation method and applications of PCR in forensics and paternity testing. Topics range from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology Each chapter includes a brief explanation of the concept and covers necessary definitions, theory and rationale for each type of calculation Recent applications of the procedures and computations in clinical, academic, industrial and basic research laboratories are cited throughout the text New to this Edition: Updated and increased coverage of real time PCR and the mathematics used to measure gene expression More sample problems in every chapter for readers to practice concepts

Author : Frank H. Stephenson
Publisher : Elsevier
Page : 302 pages
File Size : 53,5 Mb
Release : 2004-02-13
Category : Science
ISBN : 9780080500614

Get Book

Calculations for Molecular Biology and Biotechnology by Frank H. Stephenson Pdf

Calculations in Molecular Biology and Biotechnology: A Guide to Mathematics in the Laboratory is the first comprehensive guide devoted exclusively to calculations encountered in the genetic engineering laboratory. Mathematics, as a vital component of the successful design and interpretation of basic research, is used daily in laboratory work. This guide, written for students, technicians, and scientists, provides example calculations for the most frequently confronted problems encountered in gene discovery and analysis. The text and sample calculations are written in an easy-to-follow format. It is the perfect laboratory companion for anyone working in DNA manipulation and analysis. *A comprehensive guide to calculations for a wide variety of problems encountered in the basic research laboratory. * Example calculations are worked through from start to finish in easy-to-follow steps * Key chapters devoted to calculations encountered when working with bacteria, phage, PCR, radioisotopes, recombinant DNA, centrifugation, oligonucleotides, protein, and forensic science. *Written for students and laboratory technicians but a useful reference for the more experienced researcher. *A valuable teaching resource.

Author : Frank H. Stephenson
Publisher : Academic Press
Page : 320 pages
File Size : 51,6 Mb
Release : 2003-06-30
Category : Mathematics
ISBN : 9780126657517

Get Book

Calculations for Molecular Biology and Biotechnology by Frank H. Stephenson Pdf

This book provides example calculations for the most commonly encountered problems in gene discovery, analysis, and other areas of biotechnology. In addition to showing how to perform key calculations, it emphasizes mastery of basic theoretical and laboratory principles.

Author : Frank H. Stephenson
Publisher : Academic Press
Page : 496 pages
File Size : 49,5 Mb
Release : 2016-06-16
Category : Computers
ISBN : 9780128025987

Get Book

Calculations for Molecular Biology and Biotechnology by Frank H. Stephenson Pdf

Calculations in Molecular Biology and Biotechnology, Third Edition, helps researchers utilizing molecular biology and biotechnology techniques—from student to professional—understand which type of calculation to use and why. Research in biotechnology and molecular biology requires a vast amount of calculations. Results of one data set become the basis of the next. An error of choosing the wrong type of equation can turn what would have been a successful research project or weeks of labor and research into a veritable house of cards. It could be how you calculated the medium in which you test your sample to calculating how long it takes a sample to grow to calculating the synthesis of multiple variables. In one easy to use reference, Stephenson reviews the mathematics and statistics related to the day-to-day functions of biotechnology and molecular biology labs, which is a sticking point for many students, technicians, and researchers. The book covers all of the basic mathematical and statistical needs for students and professionals, providing them with a useful tool for their work. Features comprehensive calculations in biotechnology and molecular biology experiments from start to finish Provides coverage ranging from basic scientific notations to complex subjects like nucleic acid chemistry and recombinant DNA technology Includes recent applications of the procedures and computations in clinical, academic, industrial, and basic research laboratories cited throughout the text Features new coverage of digital PCR and protein quantification including chromatography and radiolabelling of proteins Includes more sample problems in every chapter for readers to practice concepts

Author : Lisa A. Seidman
Publisher : CRC Press
Page : 578 pages
File Size : 52,7 Mb
Release : 2021-12-29
Category : Medical
ISBN : 9781000480757

Get Book

Basic Laboratory Calculations for Biotechnology by Lisa A. Seidman Pdf

To succeed in the lab, it is crucial to be comfortable with the math calculations that are part of everyday work. This accessible introduction to common laboratory techniques focuses on the basics, helping even readers with good math skills to practice the most frequently encountered types of problems. Basic Laboratory Calculations for Biotechnology, Second Edition discusses very common laboratory problems, all applied to real situations. It explores multiple strategies for solving problems for a better understanding of the underlying math. Primarily organized around laboratory applications, the book begins with more general topics and moves into more specific biotechnology laboratory techniques at the end. This book features hundreds of practice problems, all with solutions and many with boxed, complete explanations; plus hundreds of "story problems" relating to real situations in the lab. Additional features include: Discusses common laboratory problems with all material applied to real situations Presents multiple strategies for solving problems help students to better understand the underlying math Provides hundreds of practice problems and their solutions Enables students to complete the material in a self-paced course structure with little teacher assistance Includes hundreds of "story problems"that relate to real situations encountered in the laboratory

Author : Frank Harold Stephenson
Publisher : Unknown
Page : 302 pages
File Size : 50,9 Mb
Release : 2004
Category : Biotechnology
ISBN : 8181474112

Get Book

Calculations For Molecular Biology & Biotechnology by Frank Harold Stephenson Pdf

Author : David J. Glass
Publisher : CSHL Press
Page : 211 pages
File Size : 49,9 Mb
Release : 2007
Category : Biology
ISBN : 9780879697358

Get Book

Experimental Design for Biologists by David J. Glass Pdf

The effective design of scientific experiments is critical to success, yet graduate students receive very little formal training in how to do it. Based on a well-received course taught by the author, Experimental Design for Biologistsfills this gap. Experimental Design for Biologistsexplains how to establish the framework for an experimental project, how to set up a system, design experiments within that system, and how to determine and use the correct set of controls. Separate chapters are devoted to negative controls, positive controls, and other categories of controls that are perhaps less recognized, such as “assumption controls†and “experimentalist controls†. Furthermore, there are sections on establishing the experimental system, which include performing critical “system controls†. Should all experimental plans be hypothesis-driven? Is a question/answer approach more appropriate? What was the hypothesis behind the Human Genome Project? What color is the sky? How does one get to Carnegie Hall? The answers to these kinds of questions can be found in Experimental Design for Biologists. Written in an engaging manner, the book provides compelling lessons in framing an experimental question, establishing a validated system to answer the question, and deriving verifiable models from experimental data. Experimental Design for Biologistsis an essential source of theory and practical guidance in designing a research plan.

Author : Alexander J. Ninfa,David P. Ballou,Marilee Benore
Publisher : John Wiley & Sons
Page : 480 pages
File Size : 48,6 Mb
Release : 2009-05-26
Category : Science
ISBN : 9780470087664

Get Book

Fundamental Laboratory Approaches for Biochemistry and Biotechnology by Alexander J. Ninfa,David P. Ballou,Marilee Benore Pdf

Ninfa/Ballou/Benore is a solid biochemistry lab manual, dedicated to developing research skills in students, allowing them to learn techniques and develop the organizational approaches necessary to conduct laboratory research. Ninfa/Ballou/Benore focuses on basic biochemistry laboratory techniques with a few molecular biology exercises, a reflection of most courses which concentrate on traditional biochemistry experiments and techniques. The manual also includes an introduction to ethics in the laboratory, uncommon in similar manuals. Most importantly, perhaps, is the authors' three-pronged approach to encouraging students to think like a research scientist: first, the authors introduce the scientific method and the hypothesis as a framework for developing conclusive experiments; second, the manual's experiments are designed to become increasingly complex in order to teach more advanced techniques and analysis; finally, gradually, the students are required to devise their own protocols. In this way, students and instructors are able to break away from a "cookbook" approach and to think and investigate for themselves. Suitable for lower-level and upper-level courses; Ninfa spans these courses and can also be used for some first-year graduate work.

Author : Pauline M. Doran
Publisher : Elsevier
Page : 455 pages
File Size : 48,5 Mb
Release : 1995-04-03
Category : Science
ISBN : 9780080528120

Get Book

Bioprocess Engineering Principles by Pauline M. Doran Pdf

The emergence and refinement of techniques in molecular biology has changed our perceptions of medicine, agriculture and environmental management. Scientific breakthroughs in gene expression, protein engineering and cell fusion are being translated by a strengthening biotechnology industry into revolutionary new products and services. Many a student has been enticed by the promise of biotechnology and the excitement of being near the cutting edge of scientific advancement. However, graduates trained in molecular biology and cell manipulation soon realise that these techniques are only part of the picture. Reaping the full benefits of biotechnology requires manufacturing capability involving the large-scale processing of biological material. Increasingly, biotechnologists are being employed by companies to work in co-operation with chemical engineers to achieve pragmatic commercial goals. For many years aspects of biochemistry and molecular genetics have been included in chemical engineering curricula, yet there has been little attempt until recently to teach aspects of engineering applicable to process design to biotechnologists. This textbook is the first to present the principles of bioprocess engineering in a way that is accessible to biological scientists. Other texts on bioprocess engineering currently available assume that the reader already has engineering training. On the other hand, chemical engineering textbooks do not consider examples from bioprocessing, and are written almost exclusively with the petroleum and chemical industries in mind. This publication explains process analysis from an engineering point of view, but refers exclusively to the treatment of biological systems. Over 170 problems and worked examples encompass a wide range of applications, including recombinant cells, plant and animal cell cultures, immobilised catalysts as well as traditional fermentation systems. * * First book to present the principles of bioprocess engineering in a way that is accessible to biological scientists * Explains process analysis from an engineering point of view, but uses worked examples relating to biological systems * Comprehensive, single-authored * 170 problems and worked examples encompass a wide range of applications, involving recombinant plant and animal cell cultures, immobilized catalysts, and traditional fermentation systems * 13 chapters, organized according to engineering sub-disciplines, are groupled in four sections - Introduction, Material and Energy Balances, Physical Processes, and Reactions and Reactors * Each chapter includes a set of problems and exercises for the student, key references, and a list of suggestions for further reading * Includes useful appendices, detailing conversion factors, physical and chemical property data, steam tables, mathematical rules, and a list of symbols used * Suitable for course adoption - follows closely curricula used on most bioprocessing and process biotechnology courses at senior undergraduate and graduate levels.

Author : Bernard R. Glick,Jack J. Pasternak
Publisher : Unknown
Page : 724 pages
File Size : 55,9 Mb
Release : 1998
Category : Medical
ISBN : UOM:39015041995500

Get Book

Molecular Biotechnology by Bernard R. Glick,Jack J. Pasternak Pdf

The second edition explains the principles of recombinant DNA technology as well as other important techniques such as DNA sequencing, the polymerase chain reaction, and the production of monclonal antibodies.

Author : Rajan Katoch
Publisher : Springer Science & Business Media
Page : 441 pages
File Size : 53,7 Mb
Release : 2011-07-19
Category : Science
ISBN : 1441997857

Get Book

Analytical Techniques in Biochemistry and Molecular Biology by Rajan Katoch Pdf

Advances in biochemistry now allow us to control living systems in ways that were undreamt of a decade ago. This volume guides researchers and students through the full spectrum of experimental protocols used in biochemistry, plant biology and biotechnology.

Author : Keith Wilson,Andreas Hofmann,John M. Walker,Samuel Clokie
Publisher : Cambridge University Press
Page : 961 pages
File Size : 41,9 Mb
Release : 2018-04-19
Category : Medical
ISBN : 9781107162273

Get Book

Wilson and Walker's Principles and Techniques of Biochemistry and Molecular Biology by Keith Wilson,Andreas Hofmann,John M. Walker,Samuel Clokie Pdf

A major update of a best-selling textbook that introduces students to the key experimental and analytical techniques underpinning life science research.

Author : Dany Spencer Adams
Publisher : CSHL Press
Page : 292 pages
File Size : 53,9 Mb
Release : 2003
Category : Mathematics
ISBN : 0879696346

Get Book

Lab Math by Dany Spencer Adams Pdf

Work at the biology bench requires an ever-increasing knowledge of mathematical methods and formulae. This is a compilation of the most common mathematical concepts and methods in molecular biology, with clear, straightforward guidance on their application to research investigations.

Author : Keith Wilson,John Walker
Publisher : Cambridge University Press
Page : 769 pages
File Size : 40,7 Mb
Release : 2010-03-04
Category : Medical
ISBN : 9780521516358

Get Book

Principles and Techniques of Biochemistry and Molecular Biology by Keith Wilson,John Walker Pdf

Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.

Author : Teresa S. Hawley,Robert Hawley
Publisher : Springer Science & Business Media
Page : 436 pages
File Size : 41,6 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592597734

Get Book

Flow Cytometry Protocols by Teresa S. Hawley,Robert Hawley Pdf

Flow cytometry has evolved since the 1940s into a multidisciplinary field incorporating aspects of laser technology, fluid dynamics, electronics, optics, computer science, physics, chemistry, biology, and mathematics. Innovations in instrumentation, development of small lasers, discovery of new fluorochromes/fluorescent proteins, and implementation of novel methodologies have all contributed to the recent rapid expansion of flow cytometry applications. In this thoroughly revised and updated second edition of Flow Cytometry Protocols, time-proven as well as cutting-edge methods are clearly and comprehensively presented by leading experimentalists. In addition to being a valuable reference manual for experienced flow cytometrists, the editors expect this authoritative up-to-date collection to prove useful to investigators in all areas of the biological and biomedical sciences who are new to the subject. The introductory chapter provides an eloquent synopsis of the principles and diverse uses of flow cytometry, beginning with a historical perspective and ending with a view to the future. Chapters 2–22 contain step-by-step protocols of highly practical and state-of-the-art techniques. Detailed instructions and helpful tips on experimental design, as well as selection of reagents and data analysis tools, will allow researchers to readily carry out flow cytometric investigations ranging from traditional phenotypic characterizations to emerging genomics and proteomics applications. Complementing these instructive protocols is a chapter that provides a preview of the next generation of solid-state lasers, and one that describes a rapid means to validate containment of infectious aerosols generated during high-speed sorting (Chapters 23–24).