Cell Culture Technology

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Cell Culture Technology

Cornelia Kasper,Verena Charwat,Antonina Lavrentieva
Cell Culture Technology Book PDF

Author : Cornelia Kasper,Verena Charwat,Antonina Lavrentieva
Publisher : Springer
Page : 168 pages
File Size : 41,9 Mb
Release : 2018-10-10
Category : Medical
ISBN : 9783319748542

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Cell Culture Technology by Cornelia Kasper,Verena Charwat,Antonina Lavrentieva Pdf

This textbook provides an overview on current cell culture techniques, conditions, and applications specifically focusing on human cell culture. This book is based on lectures, seminars and practical courses in stem cells, tissue engineering, regenerative medicine and 3D cell culture held at the University of Natural Resources and Life Sciences Vienna BOKU and the Gottfried Wilhelm Leibniz University Hannover, complemented by contributions from international experts, and therefore delivers in a compact and clear way important theoretical, as well as practical knowledge to advanced graduate students on cell culture techniques and the current status of research. The book is written for Master students and PhD candidates in biotechnology, tissue engineering and biomedicine working with mammalian, and specifically human cells. It will be of interest to doctoral colleges, Master- and PhD programs teaching courses in this area of research.

New Insights into Cell Culture Technology

Sivakumar Joghi Thatha Gowder
New Insights into Cell Culture Technology Book PDF

Author : Sivakumar Joghi Thatha Gowder
Publisher : BoD – Books on Demand
Page : 204 pages
File Size : 44,8 Mb
Release : 2017-05-10
Category : Science
ISBN : 9789535131335

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New Insights into Cell Culture Technology by Sivakumar Joghi Thatha Gowder Pdf

The book "New Insights into Cell Culture Technology" focuses on many advanced methods and techniques concerned with cell culture. The contributing authors have discussed various developments in cell culture methods, the application of insect cells for the efficient production of heterologous proteins, the expansion of human mesenchymal stromal cells for different clinical applications, the remote sensing of cell culture experiments and concepts for the development of cell culture bioprocess, continuous production of retroviral pseudotype vectors, and the production of oncolytic measles virus vectors for cancer therapy. This book is an original contribution of experts from different parts of the globe, and the in-depth information will be a significant resource for students, scientists, and physicians who are directly dealing with cells.["Culture" is essential for human life and also the life of a cell. - Sivakumar Gowder]

Animal Cell Culture and Technology

Michael Butler
Animal Cell Culture and Technology Book PDF

Author : Michael Butler
Publisher : Garland Science
Page : 299 pages
File Size : 47,8 Mb
Release : 2004-08-02
Category : Medical
ISBN : 9781135325374

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Animal Cell Culture and Technology by Michael Butler Pdf

Animal cell culture is an important laboratory technique in the biological and medical sciences. It has become an essential tool for the study of most biochemical and physiological processes and the use of large-scale animal cell culture has become increasingly important to the commercial production of specific compounds for the pharmaceutical industry. This book describes the basic requirements for establishing and maintaining cell cultures both in the laboratory and in large-scale operations. Minimal background knowledge of the subject is assumed and therefore it will be a readable introduction to animal cell culture for undergraduates, graduates and experienced researchers. Reflecting the latest developments and trends in the field, the new topics include the latest theory of the biological clock of cell lines, the development of improved serum-free media formulations, the increased understanding of the importance and control of protein glycosylation, and the humanization of antibodies for therapeutic use.

Cell Culture Technology for Pharmaceutical and Cell-Based Therapies

Sadettin Ozturk,Wei-Shou Hu
Cell Culture Technology for Pharmaceutical and Cell Based Therapies Book PDF

Author : Sadettin Ozturk,Wei-Shou Hu
Publisher : CRC Press
Page : 776 pages
File Size : 40,6 Mb
Release : 2005-08-30
Category : Medical
ISBN : 9780849351068

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Cell Culture Technology for Pharmaceutical and Cell-Based Therapies by Sadettin Ozturk,Wei-Shou Hu Pdf

Edited by two of the most distinguished pioneers in genetic manipulation and bioprocess technology, this bestselling reference presents a comprehensive overview of current cell culture technology used in the pharmaceutical industry. Contributions from several leading researchers showcase the importance of gene discovery and genomic technology devel

Technology Platforms for 3D Cell Culture

Stefan Przyborski
Technology Platforms for 3D Cell Culture Book PDF

Author : Stefan Przyborski
Publisher : John Wiley & Sons
Page : 424 pages
File Size : 43,6 Mb
Release : 2017-05-01
Category : Science
ISBN : 9781118851500

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Technology Platforms for 3D Cell Culture by Stefan Przyborski Pdf

Technology Platforms for 3D Cell Culture: A Users Guide points to the options available to perform 3D culture, shows where such technology is available, explains how it works, and reveals how it can be used by scientists working in their own labs. Offers a comprehensive, focused guide to the current state-of-the-art technologies available for 3D cell culture Features contributions from leading developers and researchers active in 3D cell technology Gives clear instruction and guidance on performing specific 3D culture methods, along with colour illustrations and examples of where such technologies have been successfully applied Includes information on resources and technical support to help initiate the use of 3D culture methods

Large-Scale Mammalian Cell Culture

Joseph Feder
Large Scale Mammalian Cell Culture Book PDF

Author : Joseph Feder
Publisher : Elsevier
Page : 170 pages
File Size : 41,8 Mb
Release : 2012-12-02
Category : Nature
ISBN : 9780323160780

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Large-Scale Mammalian Cell Culture by Joseph Feder Pdf

Large-Scale Mammalian Cell Culture is composed of papers presented as part of a symposium sponsored by the American Chemical Society Division of Microbial and Biochemical Technology at the 188th American Chemical Society National Meeting, held at Philadelphia, Pa., on Aug. 27, 1984. A rapid development of large-scale mammalian cell culture technology for the production of biologically important molecules becomes apparent. This book looks into this technology, its potential for commercial application, and the regulatory concerns posed by its use for the production of human therapeutics.

Cell Culture Bioprocess Engineering, Second Edition

Wei-Shou Hu
Cell Culture Bioprocess Engineering Second Edition Book PDF

Author : Wei-Shou Hu
Publisher : CRC Press
Page : 466 pages
File Size : 52,6 Mb
Release : 2020-03-06
Category : Science
ISBN : 9781498762861

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Cell Culture Bioprocess Engineering, Second Edition by Wei-Shou Hu Pdf

This book is the culmination of three decades of accumulated experience in teaching biotechnology professionals. It distills the fundamental principles and essential knowledge of cell culture processes from across many different disciplines and presents them in a series of easy-to-follow, comprehensive chapters. Practicality, including technological advances and best practices, is emphasized. This second edition consists of major updates to all relevant topics contained within this work. The previous edition has been successfully used in training courses on cell culture bioprocessing over the past seven years. The format of the book is well-suited to fast-paced learning, such as is found in the intensive short course, since the key take-home messages are prominently highlighted in panels. The book is also well-suited to act as a reference guide for experienced industrial practitioners of mammalian cell cultivation for the production of biologics.

Cardiac Cell Culture Technologies

Zbigniew Brzozka,Elzbieta Jastrzebska
Cardiac Cell Culture Technologies Book PDF

Author : Zbigniew Brzozka,Elzbieta Jastrzebska
Publisher : Springer
Page : 245 pages
File Size : 51,8 Mb
Release : 2017-11-21
Category : Technology & Engineering
ISBN : 9783319706856

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Cardiac Cell Culture Technologies by Zbigniew Brzozka,Elzbieta Jastrzebska Pdf

This book provides an introduction to the biological background of heart functioning and analyzes the various materials and technologies used for the development of microfluidic systems dedicated to cell culture, with an emphasis on cardiac cells. The authors describe the characterization of microfluidic systems for cardiac cell culture and center their discussion of the use of stem cell stimulation based on four different types: electrical, biochemical, physical, and mechanical. This book is appropriate for researchers focused on on-chip technologies and heart studies, students in bioengineering and microengineering courses, and a variety of professionals, such as biotechnologists, biomedical engineers, and clinicians working in the cardiac diseases field.

Basic Cell Culture Protocols

Jeffrey W. Pollard,John M. Walker
Basic Cell Culture Protocols Book PDF

Author : Jeffrey W. Pollard,John M. Walker
Publisher : Springer Science & Business Media
Page : 477 pages
File Size : 40,5 Mb
Release : 1997
Category : Animals
ISBN : 9780896034419

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Basic Cell Culture Protocols by Jeffrey W. Pollard,John M. Walker Pdf

Now completely revised and updated from the original, much-acclaimed and bestselling first edition, Basic Cell Culture Protocols, 2nd ed. offers today's most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animal cells. Its authoritative contributors provide explicit, step-by-step instructions, along with extensive notes and tips that allow both experts and beginners to successfully achieve their desired results. Topics range from basic culture methodology to strategies for culturing previously uncultured cell types and hard-to-culture differentiated cells. Methods are also provided for the analysis of living cells by FACS, video microscopy, and confocal microscopy. Like the first edition, this book should be in every cell culture laboratory and be of use to all who use cell cultures in research.

Tissue Culture Techniques

Bernice M. Martin
Tissue Culture Techniques Book PDF

Author : Bernice M. Martin
Publisher : Springer Science & Business Media
Page : 251 pages
File Size : 40,7 Mb
Release : 2013-12-01
Category : Medical
ISBN : 9781461202479

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Tissue Culture Techniques by Bernice M. Martin Pdf

ACKNOWLEDGMENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Xl II INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I STERILITY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Aseptic Technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Physical manipulations • Use of the sterile cabinet (hood) Sterilization Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 Heat • Radiation • Toxic gas • Filtration • Antibiotics Quality Control of Sterilization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 Routine labeling Suggested Readings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 vi CONTENTS ROUTINE CELL CULTURE . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 Feeding Schedules and Media Components . . . . . . . . . . . . . . . . . . . . 29 General properties of media and salt solutions • water as a reagent· Establishingfeeding schedules Subcultivation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 Solutions and methods for adherent cells • Common enzyme solutions • Inoculating (seeding) the cultures Cell Enumeration and Cell Viability . . . . . . . . . . . . . . . . . . . . . . . . . . 54 Hemocytometer • Particle counter • Cell viability Putting Routine Methods to Work . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 Normal cell growth characteristics Detecting and Disposing of Contamination . . . . . . . . . . . . . . . . . . . . 66 Bacteria and fUngi • Fungi • Mycoplasma • Viruses • Dealing with contamination Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73 Inadequate cell growth • Recurrent contamination • When to call your vendor Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 Biological hazards • Chemical hazards Suggested Readings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 Problem Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 EXPERIMENTS IN CULTURE . . . . . . . . . . . . . . . . . . . . . . . . . . 91 II Alterations of the Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 Serum • Treatments of serum • Plasma-derived serum • Serum-free and low-protein media Substrata. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101 Coatingplasticware with solutions • Alterations with polymers • Using cells to coat the plasticware • Culturing cells on microcarriers Altering the Environment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106 Temperature changes • Gaseous changes Problem Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 CONTENTS vii PRIMARY CELL CULTURE. . . . . . . . . . . . . . . . . . . . . . . . . . . . 113 Isolation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114 Dissection • Enzymatic dissociation methods • Nonenzymatic isolation • Purification of cell suspensions • Consideringyield and survival Chatacterization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Animal Cell Culture and Technology

Michael Butler
Animal Cell Culture and Technology Book PDF

Author : Michael Butler
Publisher : Taylor & Francis
Page : 299 pages
File Size : 46,5 Mb
Release : 2004-08-02
Category : Medical
ISBN : 9781135325381

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Animal Cell Culture and Technology by Michael Butler Pdf

Provides all essential practical information for establishing a laboratory animal cell culture. Comprehensive glossary of terms.

Principles and Techniques of Biochemistry and Molecular Biology

Keith Wilson,John Walker
Principles and Techniques of Biochemistry and Molecular Biology Book PDF

Author : Keith Wilson,John Walker
Publisher : Cambridge University Press
Page : 769 pages
File Size : 45,9 Mb
Release : 2010-03-04
Category : Medical
ISBN : 9780521516358

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Principles and Techniques of Biochemistry and Molecular Biology by Keith Wilson,John Walker Pdf

Uniquely integrates the theory and practice of key experimental techniques for bioscience undergraduates. Now includes drug discovery and clinical biochemistry.

Large-Scale Mammalian Cell Culture Technology

Lubiniecki
Large Scale Mammalian Cell Culture Technology Book PDF

Author : Lubiniecki
Publisher : Routledge
Page : 656 pages
File Size : 48,9 Mb
Release : 2018-05-02
Category : Medical
ISBN : 9781351435710

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Large-Scale Mammalian Cell Culture Technology by Lubiniecki Pdf

An interdisciplinary approach, integrating biochemistry, biology, genetics, and engineering for the effective production of protein pharmaceuticals. The volume offers a biological perspective of large-scale animal cell culture and examines diverse processing strategies, process management, regulator

Culture of Animal Cells

R. Ian Freshney
Culture of Animal Cells Book PDF

Author : R. Ian Freshney
Publisher : Wiley-Liss
Page : 510 pages
File Size : 41,8 Mb
Release : 1993-12-29
Category : Science
ISBN : 0471589667

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Culture of Animal Cells by R. Ian Freshney Pdf

This masterful third edition of Freshney's Culture of Animal Cells updates and considerably expands the scope of its predecessor and still enables both the novice and the experiences researcher to apply the basic and more sophisticated techniques of tissue culture. New Topics covered include: the use of molecular techniques in cell culture, such as DNA fingerprinting, fluorescence in situ hybridization, and chromosome painting cell interactions in cell culture new methods for separating cells new or refined methods for accessing cytotoxicity, viability, and mutagenicity experimental details for culture of specialized cells types not covered in previous editions new or refined techniques for visualizing clues, including time-lapse photography and confocal microscopy The revised and expanded third edition offers the following features: over 350 new reference to the primary literature an international list of cell banks an international listing of reagants and commercial supplies a subject index a glossary Also available: 0471169021 Culture of Animal Cells: A Multimedia Guide CD-ROM $150 est. From the reviews: "I strongly recommend this volume for any laboratory wishing to culture mammalian cells" - Biotechnology "It is not very often that it is possible to say of a book, 'I don't know how I managed without it previously.' Here is such a book" - Cell Biology International Reports

Cell Culture and Its Application

Ronald T. Acton,J. Daniel Lynn
Cell Culture and Its Application Book PDF

Author : Ronald T. Acton,J. Daniel Lynn
Publisher : Unknown
Page : 784 pages
File Size : 49,5 Mb
Release : 1977
Category : Science
ISBN : UOM:39015000281983

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Cell Culture and Its Application by Ronald T. Acton,J. Daniel Lynn Pdf

Cell Culture and Its Application covers the proceedings of the First International Cell Culture Congress Symposium, which focuses on how cell culture technology could impact on cell biology. The symposium aims to establish facilities for the cultivation of mammalian cells, which in turn would hopefully enhance basic cell biology research. The book is organized into four symposium and workshop sessions, encompassing 45 chapters. The opening chapter recognizes the interlocking relationship of cell culture technology and substantive cell biology. Chapters 2-5 describe the biochemical events that ...