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Chlamydomonas: Molecular Genetics and Physiology by Michael Hippler Pdf
This Microbiology Monographs volume covers the current and most recent advances in genomics and genetics, biochemistry, physiology, and molecular biology of C. reinhardtii. Expert international scientists contribute with reviews on the genome, post-genomic techniques, the genetic toolbox development as well as new insights in regulation of photosynthesis and acclimation strategies towards environmental stresses and other structural and genetic aspects, including applicable aspects in biotechnology and biomedicine. Powerful new strategies in functional genomic and genetics combined with biochemical and physiological analyses revealed new insights into Chlamydomonas biology.
The Chlamydomonas Sourcebook by Ursula Goodenough Pdf
The Chlamydomonas Sourcebook, 3rd Edition Introduction to Chlamydomonas and Its Laboratory Use (Volume 1) The gold-standard reference?covering the basic biology of the Chlamydomonas alga and techniques for its laboratory analysis Originally published as the standalone Chlamydomonas Sourcebook, then expanded as the first volume in a three-part comprehensive gold-standard reference,?The Chlamydomonas Sourcebook: Introduction to Chlamydomonas and Its Laboratory Use?has been fully revised and updated to include a?wealth of new resources for the Chlamydomonas community. Early chapters cover current understandings of its taxonomy, ultrastructure, cell and life cycles, and nuclear and organelle genomes, followed by technique-oriented chapters covering such topics as cell culture, mutagenesis, genetic analysis, construction of mutant libraries, and protein localization using immunofluorescence. This volume presents the latest in research and best practices, making it a must-have resource for researchers and students working in plant science and photosynthesis, fertility, mammalian vision, and biochemistry; crop scientists; plant physiologists; and plant, molecular, and human disease biologists. Remains the only complete reference to provide both the historical background and the most up-to-date information and applications on Chlamydomonas Includes best practices for applications in research, including methods for culture, genetic analysis, genomic and transcriptomic analysis, and mutant screening Helps researchers solve common laboratory problems, provides details on the properties of particular strains, and offers a comprehensive survey of molecular approaches Provides a broad perspective for studies in cell and molecular biology, genetics, plant physiology, and related fields
Chlamydomonas: Biotechnology and Biomedicine by Michael Hippler Pdf
This Microbiology Monographs volume covers the current and most recent advances in genomics and genetics, biochemistry, physiology, and molecular biology of C. reinhardtii. Expert international scientists contribute with reviews on the genome, post-genomic techniques, the genetic toolbox development as well as new insights in regulation of photosynthesis and acclimation strategies towards environmental stresses and other structural and genetic aspects, including applicable aspects in biotechnology and biomedicine. Advancement in Chlamydomonas biology allowed new understandings in biotechnological and biomedical related aspects.
The Chlamydomonas Sourcebook by Elizabeth H. Harris Pdf
The green alga Chlamydomonas is widely used as an experimental model system for studies in cellular and molecular biology, and in particular plant molecular biology. This book is the only single modern compendium of information on its biology and in particular its molecular biology and genetics. Included in addition to much information on the basic biology is material of a very practical nature, namely, methods for culture, preservation of cultures, preparation of media, lists of inhibitors and other additives to culture media, help with common laboratory problems such as contamination, student demonstrations, and properties of particular strains and mutants. Casual users as well as specialists will find the book to be useful in many ways. Key Features * Provides access to previously unpublished data from genetic analysis * Provides descriptions of mutant strains * Depicts summary tables comparing properties of different species and their mutant strains * Explains detailed methods for laboratory procedures of general utility * Furnishes comparisons of culture media * Presents lists of inhibitors, mutagens, and other additives to culture media * Assists with common laboratory problems such as contamination and storage of strains * Demonstrates protocols for laboratory demonstrations available for undergraduate teaching.
The Chlamydomonas Sourcebook: Introduction to Chlamydomonas and Its Laboratory Use by Elizabeth H. Harris Pdf
Chlamydomonas reinhardtii (C. reinhardtii) is a unicellular green alga whose simple life cycle and ease of growth and manipulation for genetic analysis have made it a popular organism for research in diverse areas of cell biology and genetics. C. reinhardtii is important to a variety o research from studies of flagellar biogenesis and function that are highly relevant to medical research on sperm motility and ciliary function to work on chloroplast biogenesis and photosynthesis with critical importance for agriculture. In recent years it has also proved to be an outstanding model for investigation of signal transduction, rhodopsin-based vision, and the evolution of sexual processes. The Chlamydomonas Sourcebook, published in 1989 by Academic Press, summarized the development of this alga as a laboratory system beginning in the 1940s, reviewed each major research area, as well as providing methods for growth, genetic analysis, and preparation of cellular fractions. Since the first edition published in 1989, Chlamydomonas research has grown significantly. This revised three-volume set which includes the Sourcebook, Chlamydomonas Metabolic Processes, and Chlamydomonas Motility and Taxis Responses provides the most comprehensive reference for this important research organism. The reference retains historical material, culture methods and some illustrations from the original book while adding molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism. * Expanded revision of gold standard reference * Includes latest advances in research including completion of the genome * Provides broad perspective with for studies in cell and molecular biology, genetics, plant physiology and related fields.
The Chlamydomonas Sourcebook by Arthur Grossman,Francis-André Wollman Pdf
Originally published as the stand-alone Chlamydomonas Sourcebook, then expanded as the second volume in a three-part comprehensive gold-standard reference, The Chlamydomonas Sourcebook: Organellar and Metabolic Processes has been fully revised and updated to include a wealth of new knowledge and resources for the Chlamydomonas community. It details the tremendous progress recently made with respect to imaging the ultrastructure of cells, dissecting acclimation and biosynthetic responses, and elucidating molecular processes underlying the biology of organelles. In particular, this volume includes exciting new developments in the use of imaging technologies for examining supramolecular organization of the chloroplast, defining mechanisms of branched electron transfer pathways in photosynthesis, dissecting the organization of pyrenoids and CO2 concentration mechanisms, presenting the intricacies associated with acclimation to environmental conditions and providing new insights into dark metabolism and the network of fermentative metabolism. This book thus presents the latest advances in both the research and uses of new experimental approaches and technologies, making this a must-have resource for researchers and students working in plant science and photosynthesis, fertility, mammalian vision, aspects of human disease, acclimation to environmental change, and the biogenesis of cellular complexes. Describes molecular techniques, analysis of the recently sequenced genome, reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism Provides methods for Chlamydomonas research and best practices for their applications; this includes methods for cell culture, preservation of cultures, preparation of media, lists of inhibitors, and other additives to culture media, classical genetic manipulation, and new approaches for gene transfer and editing technologies Assists researchers with common laboratory problems such as contamination
The Chlamydomonas Sourcebook 3-Vol Set by Elizabeth H. Harris Pdf
In recent years Chlamydomonas reinhardtii (C. reinhardtii) has proved to be an outstanding model for investigation of signal transduction, rhodopsin-based vision, and the evolution of sexual processes. The Chlamydomonas Sourcebook, first published in 1989, summarized the development of this alga as a laboratory system beginning in the 1940s. Since the first edition published, Chlamydomonas research has grown significantly. This revised three-volume set, which includes the Sourcebook, Chlamydomonas Metabolic Processes, and Chlamydomonas Motility and Taxis Responses stands as the most comprehensive reference for this important research organism. This set retains historical material, and culture methods and illustrations from the original book, while adding molecular techniques, analysis of the recently sequenced genome, and reviews of the current status of the diverse fields in which Chlamydomonas is used as a model organism. Edited by the leading researcher in chlamydomonas science Includes best practices for applications in research, including methods for culture, preservation of cultures, preparation of media, lists of inhibitors, and more Aids researchers with common laboratory problems such as contamination and details on properties of particular strains and mutants The latest advances in research including completion of the genome A broad perspective for studies in cell and molecular biology, genetics, plant physiology and related fields
The Molecular Biology of Chloroplasts and Mitochondria in Chlamydomonas by J.-D. Rochaix,Michel Goldschmidt-Clermont,Sabeeha Merchant Pdf
The Molecular Biology of Chloroplasts and Mitochondria in Chlamydomonas provides a comprehensive coverage of chloroplast and mitochondrial biogenesis and function. The book presents the current status of research on the molecular genetics of chloroplast gene expression. It describes the intricate cooperative interplay between the chloroplast and nuclear compartments which underlies the assembly of the photosynthetic apparatus. It also shows the power of chloroplast reverse genetics which has been used with considerable success to investigate structure-function relationships in several photosynthetic complexes. The book highlights the features of Chlamydomonas and the technological advances which make the alga uniquely suited to a multidisciplinary approach to study photosynthesis and its regulation under various environmental and stress conditions. The book is intended for a wide audience, but is specifically designed for advanced undergraduate and graduate students and researchers in the fields of biochemistry, molecular biology, physiology, biophysics, plant biology, phycology and biotechnology.
Genomic Insights into the Biology of Algae by Anonim Pdf
Advances in Botanical Research publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences. The series features a wide range of reviews by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology and ecology. This thematic volume features reviews on Genomic Insights into the Biology of Algae. Advances in Botanical Research publishes in-depth and up-to-date reviews on a wide range of topics in plant sciences Features a wide range of reviews by recognized experts on all aspects of plant genetics, biochemistry, cell biology, molecular biology, physiology and ecology This thematic volume features reviews on Genomic Insights into the Biology of Algae
The Chlamydomonas Sourcebook, 3rd Edition Cell Motility and Behavior (Volume 3) The gold-standard reference?introducing this multidisciplinary science,?fully revised and updated with the latest discoveries Originally published as the standalone Chlamydomonas Sourcebook, then expanded as the third volume in a three-part comprehensive gold-standard reference,?The Chlamydomonas Sourcebook: Cell Motility and Behavior has been fully revised and updated to include the wealth of new resources for the Chlamydomonas community. Reflecting the significant advancement in?the understanding of the role of basal bodies and cilia play in human diseases, this volume employs quantitative proteomics and mass spectroscopy as well as?cryo?EM tomography and single particle?cryo?EM. Other topics such as current insights?on?mitosis and cytokinesis, ciliary assembly and motility, intraflagellar?transport, and more help build an understanding of human diseases of the cilium. Cell Motility and Behavior presents the latest in research and best practices, making this a must-have resource for researchers and students working in plant science and photosynthesis, fertility, mammalian vision, and biochemistry; crop scientists; plant physiologists; and plant, molecular, and human disease biologists. Provides an essential reference to a model species for the study of mechanisms of motility in free living cells? Includes methods for Chlamydomonas motility research? Includes a table listing the known proteins (with NCBI accession numbers) for each structure discussed, and the known mutations that affect each structure and process
Cilia: Model Organisms and Intraflagellar Transport by Anonim Pdf
Cilia are highly conserved organelles that serve motile functions, sensory functions, or both. These organelles power cell movement, generate fluid flow in various organs, act as sensors of the extracellular environment and have been modified for various specialized tasks such as light reception and smell. Defects in these ubiquitous organelles lead to a broad array of human genetic disorders that range from polycystic kidney disease, retinal degeneration, epilepsy and infertility to developmental defects such as situs inversus and polydactyly. This volume is the third in a three-part series on cilia that focuses on the use of model organisms to gain insight into ciliary function and on the process of intraflagellar transport that is essential for the assembly and maintenance of ciliary structures. Includes both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time Covers forward and reverse genetic analysis of IFT and biochemical methods to define the role of IFT components Methods presented cover molecular, genetic, and biochemical approaches to ciliary function in model organisms
Along with its companion volume on intraflagellar transport, this book provides researchers with a comprehensive and up-to-date source of methods for the analysis cilia and flagella, focusing primarily on approaches that have been devised or significantly extended since the last volume of Methods in Cell Biology on this topic (volume 47, 1995). Edited by Stephen M. King and Gregory J. Pazour, the newest installment of this highly acclaimed serial will serve as an essential addition to the study of cilia and flagella. * Covers protocols for cilia and flagella across systems and species * Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time * Relevant to clinicians interested in respiratory disease, male infertility, and other syndromes who need to learn biochemical, molecular, and genetic approaches to studying cilia, flagella, and related structures
Molecular Genetics of Photosynthesis by Bertil Andersson,A. Hugh Salter,James Barber Pdf
Photosynthesis is one of the most active areas of plant science research. Advances in molecular biology technology have profoundly affected the work in this area and have opened up the possibility of manipulating the genes involved in photosynthesis. The field is expanding, and this book provides a compilation of authoritative reviews covering a variety of important issues.
Progress in Botany by Karl Esser,Ulrich Lüttge,Wolfram Beyschlag,Frank Hellwig Pdf
With one volume each year, this series keeps scientists and advanced students informed of the latest developments and results in all areas of the plant sciences. The present volume includes reviews on genetics, cell biology, physiology, comparative morphology, systematics, ecology, and vegetation science.
