Chromosome Techniques

Author : Arun Sharma,Archana Sharma
Publisher : Butterworth-Heinemann
Page : 724 pages
File Size : 50,7 Mb
Release : 2014-04-24
Category : Science
ISBN : 9781483100845

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Chromosome Techniques by Arun Sharma,Archana Sharma Pdf

Chromosome Techniques: Theory and Practice, Third Edition focuses on chromosome research. The book first discusses pre-treatment and hypotonic treatment. Pre-treatment for clearing the cytoplasm and softening the tissues; separation of chromosomes and clarification of constrictions; and hypotonic treatment for chromosome spread are described. The text also explains fixation and processing, including fixing of fluids and mixtures and air-drying techniques for chromosome study. The selection also discusses methods for special materials. Study of division in embryosac mother cells; study of chromosomes from thallophytes; salivary gland, lamp brush, and pachytene chromosomes; spiral structure; and secondary constriction are explained. The text also discusses microscopy, including ordinary light microscopy, high resolution autoradiography, and light microscope autoradiography. The book discusses study of plant chromosomes from tissue culture; chromosome analysis following short- and long-term cultures in animals, including man; and chromosome analysis from malignant tissues. The text takes a look at the banding patterns of chromosomes, including banding pattern techniques, C-banding, and representative schedules for comparative study of different banding patterns. The book further describes somatic cell fusion and the chemical nature of chromosomes, proteins, and enzymes. The text is a vital source of information for readers wanting to conduct research on chromosomes.

Author : Archarna Sharma
Publisher : CRC Press
Page : 392 pages
File Size : 53,5 Mb
Release : 2020-08-18
Category : Science
ISBN : 9781000124651

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Chromosome Techniques by Archarna Sharma Pdf

This laboratory manual covers the study of chromosomes in plants, animal and human systems, dealing with the protocols and principles involved. It caters to the requirements of scientists working laboratories, presenting details of the operational mechanism for use at the chromosome level.

Author : Kenneth W. Adolph
Publisher : CRC Press
Page : 488 pages
File Size : 49,5 Mb
Release : 1991-03-28
Category : Science
ISBN : 0824784308

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Advanced Techniques in Chromosome Research by Kenneth W. Adolph Pdf

Contributors detail up-to-date guidelines for using molecular techniques, cytogenetic and linkage analysis, and cellular methods, emphasizing human cells and medically relevant research. They present results of recent applications of techniques and step-by-step protocols for cloning large DNA molecu

Author : Arun Kumar Sharma
Publisher : Unknown
Page : 474 pages
File Size : 48,7 Mb
Release : 1965
Category : Biology
ISBN : LCCN:66012328

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Chromosome techniques by Arun Kumar Sharma Pdf

Author : Kiichi Fukui,Shigeki Nakayama
Publisher : CRC Press
Page : 288 pages
File Size : 45,5 Mb
Release : 2017-12-14
Category : Science
ISBN : 9781351424158

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Plant Chromosomes by Kiichi Fukui,Shigeki Nakayama Pdf

Finally - a guide to cytological techniques written specifically for the plant chromosome researcher and student. Plant Chromosomes: Laboratory Methods thoroughly covers all important approaches to the study of plant chromosomes. It reviews each specific approach and describes requisite experimental techniques. These practical descriptions cover basic, standard techniques as well as the most recent research advances and state-of-the-art technologies. Plant Chromosomes: Laboratory Methods allows you to build on the knowledge of its expert authors, who have first-hand experience with the ins and outs of each approach. Through hundreds of trouble-shooting suggestions it also helps you avoid experimental pitfalls by providing invaluable tips at critical points in the experimental process. This book gives you the information you need to improve the power of your plant chromosome research - saving you time and effort in the process. No other single volume contains so much practical information on this topic.

Author : Jorge J. Yunis
Publisher : Academic Press
Page : 272 pages
File Size : 54,7 Mb
Release : 2016-01-22
Category : Medical
ISBN : 9781483266312

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Human Chromosome Methodology by Jorge J. Yunis Pdf

Human Chromosome Methodology fills the need for an authoritative and up-to-date treatise which would serve as a text and reference for advances in human cytogenetics. The book includes readily comprehensible chapters that cover each phase of laboratory investigation from the preparation of materials for sex chromatin and chromosome techniques for bone marrow, blood, skin, and gonadal specimens to the subject of autoradiography and chromosome identification. Included also are guides to microscopy and photomicrography as well as an up-to-date treatment of chromosomes in disease. It is hoped that this volume will serve as an adequate guide to laboratory techniques and their applications for research workers, students of genetics, and members of the medical profession involved in setting up a laboratory of cytogenetics.

Author : Archarna Sharma
Publisher : CRC Press
Page : 400 pages
File Size : 50,5 Mb
Release : 2020-08-18
Category : Science
ISBN : 9781000159479

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Chromosome Techniques by Archarna Sharma Pdf

This laboratory manual covers the study of chromosomes in plants, animal and human systems, dealing with the protocols and principles involved. It caters to the requirements of scientists working laboratories, presenting details of the operational mechanism for use at the chromosome level.

Author : Arunkumar Sharma,Sharma Arun Kumar
Publisher : Unknown
Page : 711 pages
File Size : 46,6 Mb
Release : 1979
Category : Electronic
ISBN : OCLC:756559441

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Chromosome Techniques by Arunkumar Sharma,Sharma Arun Kumar Pdf

Author : John R. Gosden
Publisher : Springer Science & Business Media
Page : 508 pages
File Size : 53,5 Mb
Release : 2008-02-02
Category : Medical
ISBN : 9781592595167

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Chromosome Analysis Protocols by John R. Gosden Pdf

Chromosomes, as the genetic vehicles, provide the basic material for a large proportion of genetic investigations, from the construction of gene maps and models of chromosome organization, to the inves tigation of gene function and dysfunction. The study of chromosomes has developed in parallel with other aspects of molecular genetics, beginning with the first preparations of chromosomes from animal cells, through the development of banding techniques, which permitted the unequivocal identification of each chromosome in a karyotype, to the present analytical methods of molecular cytogenetics. Although some of these techniques have been in use for many years, and can be learned relatively easily, most published scientific reports—as a result of pressure on space from editors, and the response to that pressure by authors—contain little in the way of technical detail, and thus are rarely adequate for a researcher hoping to find all the necessary information to embark on a method from scratch. A new user needs not only a detailed description of the methods, but also some help with problem solving and sorting out the difficulties en countered in handling any biological system. This was the require ment to which the series Methods in Molecular Biology is addressed, and Chromosome Analysis Protocols forms a part of this series.

Author : Tariq Ahmad Bhat,Aijaz Ahmad Wani
Publisher : Springer
Page : 367 pages
File Size : 45,8 Mb
Release : 2017-02-08
Category : Medical
ISBN : 9788132236733

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Chromosome Structure and Aberrations by Tariq Ahmad Bhat,Aijaz Ahmad Wani Pdf

This book is a compilation of various chapters contributed by a group of leading researchers from different countries and covering up to date information based on published reports and personal experience of authors in the field of cytogenetics. Beginning with the introduction of chromosome, the subsequent chapters on organization of genetic material, karyotype evolution, structural and numerical variations in chromosomes, B-chromosomes and chromosomal aberrations provide an in-depth knowledge and easy understanding of the subject matter. A special feature of the book is the inclusion of a series of chapters on various types of chromosomal aberrations and their impact on breeding behaviour and crop improvement. The possible mechanism, their consequences and role in genetic analysis has been emphasized in these chapters. A few chapters have also been dedicated on various techniques routinely used in the laboratory by students and researchers. Each chapter ends with an extensive bibliography so that the students and researchers may find it relevant to consult more literature on the subject than a book of this size can offer. The book is intended to fulfill the needs of undergraduate and post graduate students of botany, zoology and agriculture besides, teachers and researchers engaged in the field of genetics, cytogenetics, and molecular genetics. In general the readers will find each chapter of the book informative and easy to understand.

Author : Jorge Yunis
Publisher : Elsevier
Page : 349 pages
File Size : 51,8 Mb
Release : 2012-12-02
Category : Medical
ISBN : 9780323154833

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Molecular Structure of Human Chromosomes by Jorge Yunis Pdf

Molecular Structure of Human Chromosomes is an authoritative guide to genetics, focusing on human genome. This reference compiles contributions covering available knowledge on human genome structure and organization, which the previous researches fail to encompass. This text provides a comprehensive discussion of cytogenetic techniques, emphasizing their application to human genome studies and examinations. The book is divided into nine chapters. It explains the molecular organization and function of the human genome and the DNA sequences in man. It also discusses the localization of human gene by in situ hybridization and the approaches to gene mapping. The book describes the structure of the chromosomes and the trends in chromosome techniques; banding and polymorphism; and repetitive DNA and primate evolution. Various practitioners in genetics and biology will find this book a good reference. Students and novices in these fields will also find this book an excellent guide.

Author : Beatrice Bodega,Chiara Lanzuolo
Publisher : Humana
Page : 322 pages
File Size : 54,5 Mb
Release : 2020-10-29
Category : Science
ISBN : 1071606638

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Capturing Chromosome Conformation by Beatrice Bodega,Chiara Lanzuolo Pdf

This detailed book collects methods based on the evolution of the chromosome conformation capture (3C) technique and other complementary approaches to dissect chromatin conformation with an emphasis on dissection of nuclear compartmentalization and visualization in imaging. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Capturing Chromosome Conformation: Methods and Protocols serves as an ideal guide for researchers working to further understand 3D genome organization.

Author : Arun Kumar Sharma
Publisher : Unknown
Page : 711 pages
File Size : 47,8 Mb
Release : 1990
Category : Electronic
ISBN : OCLC:708542014

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Chromosome Techniques by Arun Kumar Sharma Pdf

Author : Torbjoern Caspersson
Publisher : Elsevier
Page : 357 pages
File Size : 46,7 Mb
Release : 1973-01-01
Category : Science
ISBN : 9780323162678

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Chromosome identification: Medicine and Natural Sciences by Torbjoern Caspersson Pdf

Chromosome Identification—Technique and Applications in Biology and Medicine contains the proceedings of the Twenty-Third Nobel Symposium held at the Royal Swedish Academy of Sciences in Stockholm, Sweden, on September 25-27,1972. The papers review advances in chromosome banding techniques and their applications in biology and medicine. Techniques for the study of pattern constancy and for rapid karyotype analysis are discussed, along with cytological procedures; karyotypes in different organisms; somatic cell hybridization; and chemical composition of chromosomes. This book is comprised of 51 chapters divided into nine sections and begins with a survey of the cytological procedures, including fluorescence banding techniques, constitutive heterochromatin (C-band) technique, and Giemsa banding technique. The following chapters explore computerized statistical analysis of banding pattern; the use of distribution functions to describe integrated profiles of human chromosomes; the uniqueness of the human karyotype; and the application of somatic cell hybridization to the study of gene linkage and complementation. The mechanisms for certain chromosome aberration are also analyzed, together with fluorescent banding agents and differential staining of human chromosomes after oxidation treatment. This monograph will be of interest to practitioners in the fields of biology and medicine.

Author : National Research Council,Division on Earth and Life Studies,Commission on Life Sciences,Committee on Mapping and Sequencing the Human Genome
Publisher : National Academies Press
Page : 128 pages
File Size : 51,5 Mb
Release : 1988-01-01
Category : Science
ISBN : 9780309038409

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Mapping and Sequencing the Human Genome by National Research Council,Division on Earth and Life Studies,Commission on Life Sciences,Committee on Mapping and Sequencing the Human Genome Pdf

There is growing enthusiasm in the scientific community about the prospect of mapping and sequencing the human genome, a monumental project that will have far-reaching consequences for medicine, biology, technology, and other fields. But how will such an effort be organized and funded? How will we develop the new technologies that are needed? What new legal, social, and ethical questions will be raised? Mapping and Sequencing the Human Genome is a blueprint for this proposed project. The authors offer a highly readable explanation of the technical aspects of genetic mapping and sequencing, and they recommend specific interim and long-range research goals, organizational strategies, and funding levels. They also outline some of the legal and social questions that might arise and urge their early consideration by policymakers.