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Cryopreservation and Freeze-Drying Protocols by John G. Day,Glyn Stacey Pdf
In addition to outlining the fundamental principles associated with the conservation of biological resources, freeze-drying and cryopreservation, this text is a compilation of cryptopreservation and freeze-drying methodologies applicable to different biological materiels, developed by expert laboratories.
Cryopreservation is the storage of cells, tissues and organs at ultra-low temperatures (-196 °C) and can be an important tool for long-term conservation of plant genetic resources. A wide range of cryogenic procedures have been developed for in vitro explants, dormant buds and non-orthodox seeds in many plant species; however, research that provides novelty, insight and advances for developing and improving new protocols in plant species is still needed. This book includes the development of new protocols, physical and chemical studies related to preservation in liquid nitrogen, and some reviews on several aspects of cryopreservation. This publication will be a useful document for the community of cryopreservation scientists.
Welcomed as liberation and dismissed as exploitation, egg freezing (oocyte cryopreservation) has rapidly become one of the most widely-discussed and influential new reproductive technologies of this century. In Freezing Fertility, Lucy van de Wiel takes us inside the world of fertility preservation—with its egg freezing parties, contested age limits, proactive anticipations and equity investments—and shows how the popularization of egg freezing has profound consequences for the way in which female fertility and reproductive aging are understood, commercialized and politicized. Beyond an individual reproductive choice for people who may want to have children later in life, Freezing Fertility explores how the rise of egg freezing also reveals broader cultural, political and economic negotiations about reproductive politics, gender inequities, age normativities and the financialization of healthcare. Van de Wiel investigates these issues by analyzing a wide range of sources—varying from sparkly online platforms to heart-breaking court cases and intimate autobiographical accounts—that are emblematic of each stage of the egg freezing procedure. By following the egg’s journey, Freezing Fertility examines how contemporary egg freezing practices both reflect broader social, regulatory and economic power asymmetries and repoliticize fertility and aging in ways that affect the public at large. In doing so, the book explores how the possibility of egg freezing shifts our relation to the beginning and end of life.
Plant Cryopreservation: A Practical Guide by Barbara B.M. Reed Pdf
Cryopreservation has proven to be an important tool for the storage and conservation of plant genetic resources. This book is a unique resource for plant scientists, providing more than 100 ready-to-use cryopreservation protocols for plant types from algae and bryophytes to a range of flowering plants. It includes techniques for diverse plant parts such as dormant buds, pollen, and apical meristems and for cell types such as suspension and callus cultures.
Helps those that use cell preservation to develop new protocols or improve existing protocols This book provides readers with the tools needed to develop or debug a preservation protocol for cells. The core structure and content of the text grew from a professional short course that has been offered at the Biopreservation Core Resource for the last 10 years. This comprehensive text describes, step by step, the individual elements of a protocol, including the relevant scientific principles for each phase of the protocol. It can be used by anyone who is involved in cell preservation—even by those who are not experts in freezing of cells—because it provides the scientific basis for those that want to understand the basis for the protocol. Preservation of Cells: A Practical Manual begins by first introducing readers to the subject of preserving cells. It then goes on to cover Pre-freeze Processing and Characterization; Formulation and Introduction of Cryopreservation Solutions; Freezing Protocols; Storage and Shipping of Frozen Cells; Thawing and Post Thaw Processing; Post-thaw Assessment; and Algorithm-driven Protocol Optimization. Clearly explains the reasons behind every step in the development of a preservation protocol and the scientific principles behind them Provides alternative modes of preservation for when conventional methods of cryopreservation are not appropriate for a given cell type or application Enables more organization to achieve improved post thaw recoveries and process consistency Preservation of Cells: A Practical Manual is an important book for researchers, laboratory technicians and students in cell biology, stem cell biology, tissue engineering, and regenerative medicine. It is also useful to cell bankers, regenerative medicine, biomarker discovery or precision medicine companies, and cell therapy labs, blood bankers, biobankers, and biotechnology companies.
Current Frontiers in Cryobiology by Igor Katkov Pdf
Almost a decade has passed since the last textbook on the science of cryobiology, Life in the Frozen State, was published. Recently, there have been some serious tectonic shifts in cryobiology which were perhaps not seen on the surface but will have a profound effect on both the future of cryobiology and the development of new cryopreservation methods. We feel that it is time to revise the previous paradigms and dogmas, discuss the conceptually new cryobiological ideas, and introduce the recently emerged practical protocols for cryopreservation. The present books, "Current Frontiers in Cryobiology" and "Current Frontiers in Cryopreservation" will serve the purpose. This is a global effort by scientists from 27 countries from all continents and we hope it will be interesting to a wide audience.
Fertility Cryopreservation by Ri-Cheng Chian,Patrick Quinn Pdf
Protecting the reproductive potential of young patients undergoing cancer therapy is increasingly important. With modern treatment protocols, 80% of patients can be expected to survive. It has been estimated that up to one in 250 young adults will be a survivor of childhood cancer in the future; infertility, however, may be a consequence. As a wide range of fertility preservation methods are increasingly offered by clinicians, this systematic and comprehensive textbook dealing with the cryobiology, technology and clinical approach to this therapy will be essential reading to infertility specialists, embryologists, oncologists, cryobiologists, ObGyns, andrologists, and urologists with an interest in fertility preservation. Fertility Cryopreservation reviews all the techniques of this increasingly important field within reproductive medicine. It covers the basic principles of pertinent cryobiology, and contains major sections on the different therapies available, written by international specialists combining experience from both academic centers and commercial industries.
Cryopreservation Biotechnology in Biomedical and Biological Sciences by Yusuf Bozkurt Pdf
Cryopreservation has many biotechnological applications in different fields. This has led to an increase in importance of cryobiology as a science that examines the effect of ultra-low temperatures on cells, tissues, organs and organisms and also the freezability of these structures, while maintaining their viability. Nowadays it is well known that this form of biotechnology can be used to solve a lot of problems such as human infertility, life threatening diseases, preservation of gametes and DNA and also biodiversity conservation. Cryopreservation Biotechnology in Biomedical and Biological Sciences describes principles and application of cryopreservation biotechnology in different research areas and includes seven chapters that have been written by experts in their research fields. The chapters included in this book are thought to improve the current understanding of the different areas of using cryopreservation biotechnology.
Cryopreservation - Current Advances and Evaluations sheds light on storage of cells at subzero temperatures while ensuring that biological functionality is not compromised. Cryopreservation presents a perfect technique by which life can be preserved for posterity. However, there are many challenges to overcome and questions to answer, such as: Are organisms and metabolic systems functioning normally after cooling and thawing? This book provides comprehensive information on cryopreservation with a particular focus on cryoprotectant agents (CPAs). CPAs prevent ice from forming on cryogenically preserved cells, tissues, and organs, but can become toxic at high concentrations. As such, more research is needed to determine their precise mechanisms of action and to develop potential new CPAs that will not compromise the biology of cells. This book is an attempt in this direction.
Cryopreservation of Fish Gametes by Judith Betsy,Stephen Kumar Pdf
Understanding the reproductive physiology and endocrinology of fishes is essential for captive maturation and seed production in the field of aquaculture. Studying the spermatology of fishes is a comparatively new focus in aquaculture, which has emerged as an important area of fish research over the past two decades. In this regard, the cryopreservation of fish gametes is a crucial aspect. Moreover, energetics studies of gametes have become essential, considering the loss of vigour in the spermatozoa after cryopreservation.The latest development in this context is the cryopreservation of spermatogonial stem cell, which is also covered in the book, along with detailed information on embryo cryopreservation in fishes and crustaceans. The role of cryopreservation in conservation programmes is another important aspect, one that will especially interest biologists.This book addresses central issues in fish gamete cryopreservation and breeding, while also reviewing the history of cryopreservation. Its most unique feature is the breadth of its coverage, from basic information on reproduction in fishes, to such advanced topics as embryo cryopreservation. Chiefly intended as a handy troubleshooting guide, the book represents a valuable resource for research students in related fields.
Pollen Cryopreservation Protocols by P.E. Rajasekharan,M.R. Rohini Pdf
This volume focuses upon pollen cryopreservation in various crops from the most widely cultivated tomato to the lesser known medicinal species like Decalepis arayalpatra. Chapters detail standard techniques such as pollen collection, cryopreservation, pollen , germination tests for viability, and specific crops. Step-by-step protocols are complemented by personal notes, precautions, and specifying the reagents to be used in each step to ensure the repeatability of the procedure across labs. Authoritative and cutting-edge, Pollen Cryopreservation Protocols aims to serve as reference for researchers studying plant breeding and plant conservation biology.
Current Frontiers in Cryopreservation by Igor Katkov Pdf
Almost a decade has passed since the last textbook on the science of cryobiology, Life in the Frozen State, was published. Recently, there have been some serious tectonic shifts in cryobiology which were perhaps not seen on the surface but will have a profound effect on both the future of cryobiology and the development of new cryopreservation methods. We feel that it is time to revise the previous paradigms and dogmas, discuss the conceptually new cryobiological ideas, and introduce the recently emerged practical protocols for cryopreservation. The present books, "Current Frontiers in Cryobiology" and "Current Frontiers in Cryopreservation" will serve the purpose. This is a global effort by scientists from 27 countries from all continents and we hope it will be interesting to a wide audience.
Principles and Practice of Ovarian Tissue Cryopreservation and Transplantation by Kutluk Oktay Pdf
Principles and Practice of Ovarian Tissue Cryopreservation and Transplantation provides methods and techniques of ovarian tissue harvesting and cryopreservation, including instructional videos. This book will benefit a wide audience, guiding infertility specialists, fellows, residents, reproductive surgeons, reproductive endocrinologists, pediatric surgeons, embryologists, infertility nurses and gynecologists. Ovarian cryopreservation and transplantation is rapidly gaining acceptance as a successful and established fertility preservation strategy in cancer patients and beyond. Unlike other fertility preservation strategies, it can be performed in children as well as adults, and can be helpful in restoring natural ovarian function and fertility, hence this is a welcomed resource on the topic. Provides instructional videos, materials and an equipment list for ovarian tissue harvesting and cryopreservation, along with protocols for ovarian tissue cryopreservation thawing and transplantation Presents instructional video coverage of ovarian tissue cryopreservation for use at fertility centers around the globe Fulfills a specific niche in the literature, providing a comprehensive reference on ovarian tissue freezing and transplantation Includes a foreword written by a cancer survivor who successfully received this procedure
Cryopreservation of Plant Germplasm I by Y. P. S. Bajaj Pdf
The germ plasm of numerous plant species, especially those of forest trees, some agricultural crops, and medicinal plants, is endangered and threatened with extinction. This depletion of germplasm pools and the shrinkage of naturally occurring genetic resources have caused international concern. Conventionally, the germplasm of plants is conserved through seeds, tubers, roots, corms, rhizomes, bulbs, cuttings, etc. However, the germ plasm of a number of trees and plantation crops (such as coconut, cocao, coffee, oil palm, rubber, mango, horse chestnut, etc. ) cannot be preserved since their seed are short-lived (recalcitrant). Likewise, germplasm of vegetatively propagated crops (such as potato and cassava) cannot be stored on a long term basis and has to be grown and multiplied periodically in nurseries and fields. The plants are thus exposed to unpredictable weather conditions and diseases, with the result that instances are known where entire genetic stocks are lost. Therefore, unconventional methods are being developed for the storage and international exchange of germplasm. For this purpose in vitro cultures have been employed, but they can only enable short-to medium term preservation; moreover, cell cultures upon repeated subculture undergo genetic erosion. In view of the recent developments in the in vitro induction of genetic variability through somaclonal variation, somatic hybridization, recombinant DNA technology, etc. , new methods need to be employed for the storage of desirable cultures. In this regard freeze preservation of cells in liquid nitrogen (-196 0q, like that of semen, enables long-term storage, theoretically, for an indefinite period of time.
Gonadal Tissue Cryopreservation in Fertility Preservation by Nao Suzuki,Jacques Donnez Pdf
This book is a practical guide to the cryopreservation and transplantation of oocytes, embryos, and ovarian and testicular tissue for fertility preservation. Illustrated with a wealth of figures and photos, it provides updated techniques for clinicians, technicians, and researchers. This book also gives insights into in vitro activation (IVA), in vitro growing (IVG), and in vitro maturation (IVM), with both basic research and future directions. These techniques combined with cryopreservation will improve the chances of reproduction. Cryopreservation is essential to protect fertility in cancer patients who undergo treatment with radioiodine or chemotherapy, which may cause damage to their reproductive organs, and also for primary ovarian insufficiency patients. The book also includes a chapter on the risk of the presence of malignant cells in ovarian tissue. Both editors are pioneering researchers in this field.