Diagnostic Manual For The Identification Of Insect Pathogens

Author : George Poinar
Publisher : Springer Science & Business Media
Page : 226 pages
File Size : 48,6 Mb
Release : 2012-12-06
Category : Science
ISBN : 9781468424393

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Diagnostic Manual for the Identification of Insect Pathogens by George Poinar Pdf

This manual was prepared for the diagnosis of insect diseases caused by infectious agents. The agents (or pathogens) included here are fungi, protozoans, bacteria, viruses, and rickettsias. The present work was prepared after much deliberation and discussion with students and teachers who felt a guide of this type would be valuable for diagnosing the microbial diseases of insects. It was modeled after a seminar given on the same subject at Berke ley, which had as its major goal the recognition and identification of insect pathogens for practical purposes. The present work in cludes numerous timesaving "short cuts" which were developed after years of experience of diagnosing insect diseases. Although emphasis is placed on identification, general back ground information on the various pathogens is also included. Thus, under each of the five groups of pathogens, the following topics are discussed: (1) various types of associations with insects, (2) defini tion and classification, (3) general life cycle, (4) characteristics of diseased insects, (5) factors affecting natural infections, (6) methods of examination, (7) isolation and cultivation, (8) important taxonomic characters, (9) tests for infectivity, (10) storage, (11) an illustrated key to the genera (or group in the case of viruses), and (12) literature, especially that pertaining to identification. Although often included with insect pathogens, entomogenous nematodes are not covered here since illustrated keys to those gen era that infect insects are already available (Poinar, 1975, 1977).

Author : George Poinar
Publisher : Unknown
Page : 236 pages
File Size : 40,9 Mb
Release : 1978-02-01
Category : Electronic
ISBN : 1468424408

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Diagnostic Manual for the Identification of Insect Pathogens by George Poinar Pdf

Author : G.O. Poinar Jr.,G.M. Thomas
Publisher : Springer Science & Business Media
Page : 400 pages
File Size : 47,8 Mb
Release : 2012-12-06
Category : Science
ISBN : 9781468485448

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Laboratory Guide to Insect Pathogens and Parasites by G.O. Poinar Jr.,G.M. Thomas Pdf

After the publication of the Diagnostic Manual for the Identification of Insect Pathogens, the authors received many queries asking why they had not included the larger metazoan parasites as well as the microbial forms. An examination of the literature indicated that pictorial guides to the identification of nematodes and the immature stages of insect parasites were unavailable. Consequently we decided to rewrite the sections cover ing insect pathogens and combine these with new sections on ento mogenous nematodes and the immature stages of insect parasites. The result is the present laboratory guide, which is unique in covering all types of biotic agents which are found inside insects and cause them injury or disease. Included as parasites are insects and nematodes. Among the pathogens included are viruses, rickettsias, bacteria, fungi, and protozoans. Emphasis is placed on identification with an attempt to use the most easily recognizable characters. Use of a certain number of technical terms is unavoidable, and explanations of these can be found in most biological dictionaries or the glossary of invertebrate pathology prepared by Steinhaus and Martignoni (1970).

Author : Vladimir Gouli,Svetlana Gouli,Jose Marcelino
Publisher : Springer Science & Business Media
Page : 63 pages
File Size : 41,7 Mb
Release : 2011-08-12
Category : Science
ISBN : 940071890X

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Common Infectious Diseases of Insects in Culture by Vladimir Gouli,Svetlana Gouli,Jose Marcelino Pdf

This handbook gives a comprehensive and copious illustrated description, with original art work, of the most common diseases in laboratory reared insect colonies, comprising Viruses (Baculoviridae, Reoviridae, Poxviridae, Iridoviridae); Bacteria (Bacillaceae, Pseudomonadaceae, Enterobacteriaceae); Rickettsia; Fungi and Microsporidia and Protozoa. Gregarine and Coccidian parasitoids are also depicted. Manuals for the diagnostic of insect diseases have been published in the 90’s, however, these were intended for professional insect pathologists or for proficient training in invertebrate pathology, hence requiring some scientific background in insect pathology. Currently, most guides are web based, not comprehensive and solely addressing the most common diseases in a particular insect, or group of insects, reared in laboratory facilities. A comprehensive and proficient practical handbook for students and technicians working with insect reared colonies is lacking in the current literature.

Author : S. Patricia Stock
Publisher : CABI
Page : 435 pages
File Size : 53,8 Mb
Release : 2009-01-01
Category : Nature
ISBN : 9781845934798

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Insect Pathogens by S. Patricia Stock Pdf

This book attempts to bring together a broad array of molecular techniques and approaches currently used in insect pathology. It is divided into four parts: (i) identification and diagnostics; (ii) evolutionary relationships and genetics; (iii) host-pathogen interactions; and (iv) genomics and genetic engineering. Sixteen chapters have been written by leading researchers in the field which provide comprehensive and up-to-date information on each part.

Author : Lawrence A. Lacey
Publisher : Academic Press
Page : 513 pages
File Size : 49,6 Mb
Release : 2012-06-15
Category : Science
ISBN : 9780123868992

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Manual of Techniques in Invertebrate Pathology by Lawrence A. Lacey Pdf

1. Initial Handling and Diagnosis of Diseased Invertebrates / Lawrence A. Lacey and Leellen Solter -- 2. Basic Techniques in Insect Virology / Karolin E. Eberle, Jorg T. Wennmann, Regina G. Kleespies and Johannes A. Jehle -- 3. Isolation, Culture, Preservation, and Identification of Entomopathogenic Bacteria of the Bacilli. / Tanja W. Fisher and Steven F. Garczynski -- 4. Bioassay of Bacterial Entomopathogens Against Insect Larvae / Maureen O'Callaghan, Travis R. Glare and Lawrence A. Lacey --5. Bacteria for use Against Soil-Inhabitating Insects / Albrecht M. Koppenhofer, Trevor A. Jackson, and Michael G. Klein -- 6. Identification of Entomopathogenic Fungi / Richard A. Humber -- 7. Laboratory Techniques Used for Entomopathogenic Fungi: Hypocreales / G. Douglas Inglis, Juerg Enkerli, and Mark S. Goettel -- 8. Mass Production of Entomopathogenic Hypocreales / Stefan T. Jaronski and Mark A. Jackson -- 9. Methods for the Study of Entomophthorales / Ann E. Hajek, Bernard Papierok, and Jurg ...

Author : Lawrence A. Lacey
Publisher : Academic Press
Page : 441 pages
File Size : 44,8 Mb
Release : 1997-02-27
Category : Technology & Engineering
ISBN : 9780080535777

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Manual of Techniques in Insect Pathology by Lawrence A. Lacey Pdf

Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers. In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series. The books will be of value to advances researches and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work. This manual describes the broad array of techniques that are used in insect pathology. It will provide biologists, insect pathologists, entomologists, and those interested in biological control, with the necessary information to work on a variety of pathogen groups. This book will be an essential laboratory reference for insect pathologists. Features include: * Step by-step instructions on how to isolate, identify, culture, bioassay and store the major groups of entomopathogens * Details of the practical knowledge needed by beginners to apply the techniques * Chapters written by an international group of experts * Discussion of safety testing of entomopathogens in mammals and also broader methods such as microscopy and molecular techniques * Provides extensive supplemental literature and recipes for media, fixatives and stains

Author : J. Richard Blanchard,Lois Farrell
Publisher : Univ of California Press
Page : 748 pages
File Size : 45,8 Mb
Release : 2023-07-28
Category : Science
ISBN : 9780520328730

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Guide to Sources for Agricultural and Biological Research by J. Richard Blanchard,Lois Farrell Pdf

This title is part of UC Press's Voices Revived program, which commemorates University of California Press’s mission to seek out and cultivate the brightest minds and give them voice, reach, and impact. Drawing on a backlist dating to 1893, Voices Revived makes high-quality, peer-reviewed scholarship accessible once again using print-on-demand technology. This title was originally published in 1981.

Author : Lawrence A. Lacey,Harry K. Kaya
Publisher : Springer Science & Business Media
Page : 852 pages
File Size : 42,7 Mb
Release : 2007-09-27
Category : Science
ISBN : 9781402059339

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Field Manual of Techniques in Invertebrate Pathology by Lawrence A. Lacey,Harry K. Kaya Pdf

This field manual is designed to provide background and instruction on a broad spectrum of techniques and their use in the evaluation of entomopathogens in the field. The second edition provides updated information and includes two additional chapters and 12 new contributors. The intended audience includes researchers, graduate students, practitioners of integrated pest management (IPM), regulators and those conducting environmental impact studies of entomopathogens.

Author : Lawrence A. Lacey,Harry K. Kaya
Publisher : Springer Science & Business Media
Page : 913 pages
File Size : 44,7 Mb
Release : 2013-06-29
Category : Science
ISBN : 9789401715478

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Field Manual of Techniques in Invertebrate Pathology by Lawrence A. Lacey,Harry K. Kaya Pdf

The 38 chapters of this Field Manual provide the tools required for planning experiments with entomopathogens and their implementation in the field. Basic tools include chapters on the theory and practice of microbial control agents, statistical design of experiments, equipment and application strategies. The major pathogen groups are covered in individual chapters (virus, bacteria, protozoa, fungi, nematodes). Subsequent chapters deal with the impact of naturally occurring and introduced exotic pathogens and inundative application of microbial control agents. The largest section of the Manual is composed of 21 chapters on the application and evaluation of entomopathogens in a wide range of agricultural, forest, domestic and aquatic habitats. Mites and slugs broaden the scope of the book. Supplementary techniques and media for follow-up laboratory studies are described. Three final chapters cover the evaluation of Bt transgenic plants, resistance to insect pathogens and strategies to manage it, and guidelines for evaluating the effects of MCAs on nontarget organisms. Readership: Researchers, graduate students, practitioners of integrated pest management, regulators, those doing environmental impact studies. The book is a stand-alone reference, but is also complementary to the laboratory-oriented Manual of Techniques in Insect Pathology and similar comprehensive texts.

Author : Lawrence A. Lacey
Publisher : Academic Press
Page : 504 pages
File Size : 49,8 Mb
Release : 2012-04-25
Category : Science
ISBN : 9780123869005

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Manual of Techniques in Invertebrate Pathology by Lawrence A. Lacey Pdf

Manual of Techniques in Invertebrate Pathology, Second Edition, describes a wide range of techniques used in the identification, isolation, propagation/cultivation, bioassay, quantification, preservation, and storage of the major groups of entomopathogens, including entomophthorales, entomopathogenic fungi, entomopathogenic bacteria of the Bacilli, Nematode parasites, and pathogens and parasites of terrestrial molluscs. The book presents the perspectives of an international group of experts in the fields of invertebrate pathology, including microbiology, mycology, virology, nematology, biological control, and integrated pest management. Organized into 15 chapters, the book covers methods for the study of virtually every major group of entomopathogen, as well as methods for discovery and diagnosis of entomopathogens and the use of complementary methods for microscopy. It discusses the use of molecular techniques for identifying and determining phylogeny, factors that contribute to resistance to entomopathogens, and several other aspects of the science of invertebrate pathology. It also explains initial handling and diagnosis of diseased invertebrates, basic techniques in insect virology, and bioassay of bacterial entomopathogens against insect larvae. In addition, the reader is introduced to the use of bacteria against soil-inhabiting insects and preservation of entomopathogenic fungal cultures. The remaining chapters focus on research methods for entomopathogenic microsporidia and other protists, how the pathogenicity and infectivity of entomopathogens to mammals are tested, and preparations of entomopathogens and diseased specimens for more detailed study using microscopy. Experienced insect pathologists, biologists, entomologists, students, biotechnology personnel, technicians, those working in the biopesticide industry, and government regulators will find this manual extremely helpful. Step-by-step instructions for the latest techniques on how to isolate, identify, culture, bioassay and store the major groups of entomopathogens New edition fully updated to address changes in the taxonomy of the vast majority of taxa Discussion of safety testing of entomopathogens in mammals and also broader methods such as microscopy and molecular techniques Provides extensive supplemental literature and recipes for media, fixatives and stains

Author : Anonim
Publisher : Unknown
Page : 602 pages
File Size : 55,8 Mb
Release : 1982-05
Category : Parasites
ISBN : UCR:31210017363209

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Index-catalogue of Medical and Veterinary Zoology by Anonim Pdf

Author : M. J. Daniels
Publisher : Springer Science & Business Media
Page : 367 pages
File Size : 52,6 Mb
Release : 2012-12-06
Category : Science
ISBN : 9789401511643

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Plant and Insect Mycoplasma Techniques by M. J. Daniels Pdf

Only 14 years have passed since the first publication appeared which implicated mycoplasmas as agents of plant disease. The diseases them selves have been known for much longer; indeed clover phyllody, a typical example, was described in the seventeenth century, well before any animal mycoplasma diseases had been documented. The early history of plant mycoplasmas is described in Chapter 2 and one obvious conclusion to be drawn from the frustrating experiences of the earlier workers is that the experimental methods at their disposal were simply inadequate for the task. Progress in science depends critically upon the development of new methods. Although important advances have been made in plant and insect mycoplasmology, notably in the discovery of spiroplasmas, many intractable problems remain. Most plant myco plasmas cannot yet be cultured in vitro, and their natural plant habitat, the phloem, is one of the most difficult plant tissues for the experi menter to handle, placing severe restrictions on the type of experiments which can be performed in vivo. It is clear that radically new methods may be required to solve these problems. A survey of the progress which has been made shows that application of techniques from a wide range of disciplines has been necessary. A successful individual or group of workers must possess the skills of a plant pathologist, a plantsman, a plant physiologist, a light-and electron microscopist, a bacteriologist, a biochemist, an immunologist, an ento mologist, a virologist and a molecular geneticist.

Author : Allen Carson Cohen
Publisher : CRC Press
Page : 473 pages
File Size : 49,9 Mb
Release : 2015-06-15
Category : Nature
ISBN : 9781466591950

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Insect Diets by Allen Carson Cohen Pdf

Dr. Allen Carson Cohen’s new edition of Insect Diets: Science and Technology continues to provide a current, integrated review of the field of insect diets. It reaffirms and expands upon the belief that the science of diet development and the technology of diet application in rearing programs require formal foundations and guidelines. Cohen argues for a data-driven approach as well as a focus on humane treatment in insect rearing programs. He also calls for academics and industries to make a new push toward statistical process control (SPC) in their approaches to rearing in general, using his own work with insects as a paradigm. This approach yields the benefits of careful scientific analysis by addressing issues of quality and efficiency in academic research and industrial practices and applications. See What’s New in the Second Edition: This edition expands upon the role of food science in the use of artificial diets in rearing programs, especially texture analysis with rheological techniques. It includes an entirely new chapter focused solely on the subject of food quality in insect diets. The book also revisits microbial relationships to insect diets as a powerful influence on their feeding processes and emphasizes a new, better understanding and utilization of the relationship between insects and microbes in artificial diets. Cohen also expands his vision of the future of insect rearing, including the use of insects themselves as a potential food source for a rapidly expanding global human population. To that end, this book gives you guidelines to develop, use, and evaluate artificial diets in order to improve their cost and scientific efficiency in the rearing of insects, because as the author urges, it is important to "know your insect." This understanding will serve the multifaceted goals of using insect rearing for research and teaching, pest management strategies and biocontrol agents, as food for other organisms, and for many other purposes.

Author : T.R. Southwood
Publisher : Springer Science & Business Media
Page : 548 pages
File Size : 51,5 Mb
Release : 2013-06-29
Category : Science
ISBN : 9789401572910

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Ecological Methods by T.R. Southwood Pdf

The virtual impossibility of extracting the many different species from a habitat with equal efficiency by a single method (e.g. Nef, 1960). 1.1 Population estimates Population estimates can be classified into a number of different types; the most convenient classification is that adopted by Morris (1955), although he used the terms somewhat differently in a later paper (1960). 1.1.1 Absolute and related estimates The animal numbers may be expressed as a density per unit area of the ground of the habitat. Such estimates are given by nearest neighbour and related techniques (Chapter 2), marking and recapture (Chapter 3), by sampling a known fraction of the habitat (Chapter 4-6) and by removal sampling and random walk techniques (Chapter 7). Absolute population The number of animals per unit area (e.g. hectare, acre). It is almost impossible to construct a budget or to study mortality factors without the conversion of population estimates to absolute figures, for not only do insects often move from the plant to the soil at different developmental stages, but the amount of plant material is itself always changing. The importance of obtaining absolute estimates cannot be overemphasized.