Diagnostic Virology Protocols

Author : John R. Stephenson,Alan Warnes
Publisher : Springer Science & Business Media
Page : 388 pages
File Size : 48,9 Mb
Release : 1998-08-28
Category : Medical
ISBN : 0896034798

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Diagnostic Virology Protocols by John R. Stephenson,Alan Warnes Pdf

A collection of cutting-edge techniques for detecting most of the major viruses that afflict mankind, including influenza, hepatitis, herpes, polio, mumps, HIV, and many more. The techniques are well-tested, easily reproducible, and readily employ all the new technologies-PCR, RIA, ELISA, and latex-agglutination-that have revolutionized the field. These methods not only make it possible to do the necessary analysis in hours instead of days, but can also be automated in a laboratory havng only low levels of biological containment. Frequently, the protocols for viruses causing human diseases can be adapted to similar viruses of veterinary importance. Through its state-of-the-art methods a physician can, for the first time, determine early in a viral infection which antiviral drug should be used and minimize the period of treatment to avoid unnecessary side effects.

Author : John R. Stephenson,Alan Warnes
Publisher : Unknown
Page : 517 pages
File Size : 47,8 Mb
Release : 2020-08-23
Category : Electronic
ISBN : 9798678230232

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Diagnostic Virology Protocols by John R. Stephenson,Alan Warnes Pdf

Recent outbreaks of swine influenza and avian influenza, along with the remaining and in some cases expanding threats from HIV, dengue virus, and the viruses causing hepatitis, have reinforced the need for rapid, accurate and cost-effective diagnosis of viral disease. Diagnostic Virology Protocols, Second Edition brings the field fully up-to-date with a focus on protocols involving nucleic acid detection, most often through some form of the polymerase chain reaction (PCR). The expert contributors also delve into the key technology of robotics as well as future prospects, such as further refined point-of-care testing and the increasing importance of mathematical modelling. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Diagnostic Virology Protocols, Second Edition captures the dramatic changes in the virus diagnostic laboratory in order to better prepare scientists to combat the inevitable threats to public health from future and present infectious diseases.

Author : Danny L. Wiedbrauk,Daniel H. Farkas
Publisher : Elsevier
Page : 386 pages
File Size : 40,6 Mb
Release : 1995-02-08
Category : Science
ISBN : 0080536891

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Molecular Methods for Virus Detection by Danny L. Wiedbrauk,Daniel H. Farkas Pdf

Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods. Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies. Provides state-of-the-art PCR methods for amplification, quantitation, in situ hybridization, and multiplex reactions Goes beyond PCR with protocols for 3SR, NASBA, LCR, SDA, and LAT Covers important virus detection methods such as in situ hybridization; Southern, dot, and slot blots; branched chain signal amplification; and chemiluminescence Includes quality control information crucial in research and clinical laboratories Most chapters are written by the inventors and principal developers of the methodologies Includes color plates, 77 figures, and 18 tables

Author : Hillar O. Kangro,Brian W.J. Mahy
Publisher : Elsevier
Page : 374 pages
File Size : 40,9 Mb
Release : 1996-04-16
Category : Science
ISBN : 0080543588

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Virology Methods Manual by Hillar O. Kangro,Brian W.J. Mahy Pdf

The Virology Methods Manual is a comprehensive source of methods for the study, manipulation, and detection of viruses. Edited by Brian Mahy and Hillar Kangro, this work describes the most up-to-date, definitive techniques, provided by experts in each area, and presented with easy-to-use, step-by-step protocols. This new manual will satisfy the needs of virologists and all those working with viruses who need a practical guide to methods that work! Provides up-to-date techniques by experts worldwide Presents common, step-by-step protocols in an attractive, easy-to-use fashion Contains useful appendices including virus taxonomy, metabolic inhibitors, and Bio-safety in the virology laboratory

Author : Rajib Deb,Ajay Kumar Yadav,Swaraj Rajkhowa,Yashpal Singh Malik
Publisher : Unknown
Page : 381 pages
File Size : 53,9 Mb
Release : 2022
Category : Swine
ISBN : 1071620436

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Protocols for the Diagnosis of Pig Viral Diseases by Rajib Deb,Ajay Kumar Yadav,Swaraj Rajkhowa,Yashpal Singh Malik Pdf

This detailed book explores new molecular biological techniques for the detection of both antigens and antibodies of porcine diseases. Assays examined within this collection include improved methods of nucleic acid extraction, polymerase chain reaction (PCR), droplet digital PCR (ddPCR), polymerase spiral reaction (PSR), cross-priming amplification (CPA), enzyme-linked immunosorbent assay (ELISA), as well as peptide nucleic acid (PNA) based tools, aptamer-based tools, and lateral flow assays and different immune assay-based diagnostics for porcine diseases. As part of the Springer Protocols Handbooks series, the present book contains the type of in-depth knowledge on important diagnostics tools that provide a vital source of information for scientists, researchers, and students from different fields. Authoritative and practical, Protocols for the Diagnosis of Pig Viral Diseases aims to guide researchers toward developing new technologies for the diagnosis of pig pathogens.

Author : Justin Jang Hann Chu,Bintou Ahmadou Ahidjo,Chee Keng Mok
Publisher : Humana
Page : 478 pages
File Size : 52,9 Mb
Release : 2022-05-12
Category : Medical
ISBN : 1071621106

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SARS-CoV-2 by Justin Jang Hann Chu,Bintou Ahmadou Ahidjo,Chee Keng Mok Pdf

This detailed volume provides the increasing number of SARS-CoV-2 researchers with a useful handbook covering multidisciplinary approaches on various aspects of SARS-CoV-2 research, brought together by leading laboratories across the globe. Topics covered include techniques in clinical and diagnostic virology, basic protocols in cell and virus culture, as well as bioinformatics and proteomics approaches in cellular response studies. This comprehensive collection also covers methods in immunology, animal models, antivirals and vaccine development strategies, as well as biorisk and mitigation measurements for SARS-CoV-2 research. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detailed implementation advice that is vital for success in the lab. Practical and timely, SARS-CoV-2: Methods and Protocols serves as an ideal guide for scientists investigating this prevalent and perilous RNA virus and the novel coronavirus disease that results from it.

Author : Richard L. Hodinka,Stephen A. Young,Benjamin A. Pinksy
Publisher : John Wiley & Sons
Page : 622 pages
File Size : 44,6 Mb
Release : 2020-07-10
Category : Medical
ISBN : 9781555819156

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Clinical Virology Manual by Richard L. Hodinka,Stephen A. Young,Benjamin A. Pinksy Pdf

The definitive clinical virology resource for physicians and clinical laboratory virologists The clinical virology field is rapidly evolving and, as a result, physicians and clinical laboratory virologists must have a reliable reference tool to aid in their ability to identify and diagnose viral infections to prevent future outbreaks. In this completely revised edition of the Clinical Virology Manual, Editor in Chief, Michael Loeffelholz, along with Section Editors, Richard Hodinka, Benjamin Pinsky, and Stephen Young, have complied expert perspectives of a renowned team of clinical virology experts and divided these contributions into three sections to provide the latest information on the diagnosis of viral infections, including ebola, HIV and Human papillomavirus state of the art diagnostic technologies, including next-generation sequencing and nucleic acid amplification methods taxonomy of clinically important viruses such as polyomaviruses and zoonotic viruses This comprehensive reference also includes three appendices with vital information on reference virology laboratories at the Centers for Disease Control and Prevention, state and local public health laboratories, and international reference laboratories and laboratory systems. Additionally, a new section "Diagnostic Best Practices," which summarizes recommendations for diagnostic testing, and cites evidence-based guidelines, is included in each viral pathogens chapter. Clinical Virology Manual, Fifth Edition serves as a reference source to healthcare professionals and laboratorians in providing clinical and technical information regarding viral diseases and the diagnosis of viral infections.

Author : Norman Roy Grist,Constance A. Ross,Eleanor J. Bell
Publisher : Unknown
Page : 224 pages
File Size : 45,8 Mb
Release : 1974
Category : Diagnostic virology
ISBN : UOM:39015003830570

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Diagnostic Methods in Clinical Virology by Norman Roy Grist,Constance A. Ross,Eleanor J. Bell Pdf

Author : Helena J. Maier,Erica Bickerton
Publisher : Humana
Page : 0 pages
File Size : 55,7 Mb
Release : 2020-08-25
Category : Medical
ISBN : 1071608991

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Coronaviruses by Helena J. Maier,Erica Bickerton Pdf

This detailed new edition provides a comprehensive collection of protocols applicable to all members of the Coronavirinae sub-family currently and that are also transferrable to other fields of virology. Beginning with a section on detection, discovery, and evolution, the volume continues with coverage of propagation and titration of coronaviruses, genome manipulation, study of virus-host interactions, as well as imaging coronavirus infections. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Coronaviruses: Methods and Protocols, Second Edition serves as a valuable guide to researchers working to identify and control viruses with increased potential to cross the species barrier and to develop the diagnostics, vaccines, and antiviral therapeutics that are required to manage future outbreaks in both humans and animals.

Author : Justin Jang Hann Chu,Swee Kim Ang
Publisher : Humana
Page : 0 pages
File Size : 43,9 Mb
Release : 2016-05-28
Category : Medical
ISBN : 1493936166

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Chikungunya Virus by Justin Jang Hann Chu,Swee Kim Ang Pdf

This volume covers multidisciplinary approaches on various aspects of Chikungunya Virus (CHIKV) research that was brought together from leading laboratories across the globe. Topics covered include techniques in clinical and diagnostic virology, basic protocols in cell and virus culture, bioinformatics, and proteomics approaches in cellular response studies. Chikungunya Virus: Methods and Protocols also discusses methods in immunology and animal model studies, as well as different strategies of antivirals and vaccine development for therapeutics against CHIKV infection. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary material and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and practical, Chikungunya Virus: Methods and Protocols is a useful and comprehensive resource for CHIKV researchers and anyone interested in learning more about the Chikungunya Virus.

Author : S. K. Green,Asian Vegetable Research and Development Center
Publisher : Unknown
Page : 56 pages
File Size : 49,8 Mb
Release : 1984
Category : Agricultural virology
ISBN : UOM:39015014902145

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Guidelines for Diagnostic Work in Plant Virology by S. K. Green,Asian Vegetable Research and Development Center Pdf

Author : Reeti Khare
Publisher : Unknown
Page : 311 pages
File Size : 54,7 Mb
Release : 2019
Category : Diagnostic virology
ISBN : 1523123931

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Guide to Clinical and Diagnostic Virology by Reeti Khare Pdf

"The explosion in clinical testing has been especially rapid in virology, where emerging viruses and growing numbers of viral infections are driving advances. This digestible view of the breadth and depth of information related to clinical virology provides a practical, working knowledge of the wide array of viruses that cause human disease. Introductory chapters cover the basics of clinical virology and laboratory diagnosis of infections, including virus structure, life cycle, transmission, taxonomy, specimen types and handling, and a comparison of assays used for detection"--Publisher's description.

Author : S. E. Albrechtsen
Publisher : CABI
Page : 282 pages
File Size : 46,5 Mb
Release : 2006
Category : Science
ISBN : 9781845931483

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Testing Methods for Seed-transmitted Viruses by S. E. Albrechtsen Pdf

This practical guide covers the commonly used detection methods for seed-transmitted viruses and viroids that affect both tropical and temperate crops. It contains 25 complete step-by-step procedures for biological, serological and molecular techniques to detect and identify such viruses. Combining helpful practical notes with more detailed explanations of the principles behind the techniques, the book describes the general characteristics of seed-transmitted viral diseases and discusses outlines for the organization and interpretation of seed health assays. The techniques reviewed are also applicable to non-seed-transmitted viral agents.

Author : S. K. Green
Publisher : Unknown
Page : 63 pages
File Size : 45,7 Mb
Release : 1991
Category : Electronic
ISBN : OCLC:694988125

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Guidelines for Diagnostic Work in Plant Virology by S. K. Green Pdf

Author : Justin Jang Hann Chu,Swee Kim Ang
Publisher : Methods in Molecular Biology
Page : 352 pages
File Size : 43,8 Mb
Release : 2018-06-11
Category : Medical
ISBN : 1493980963

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Chikungunya Virus: Methods and Protocols by Justin Jang Hann Chu,Swee Kim Ang Pdf