Dynamic Force Spectroscopy And Biomolecular Recognition

Author : Anna Rita Bizzarri,Salvatore Cannistraro
Publisher : CRC Press
Page : 270 pages
File Size : 45,6 Mb
Release : 2012-01-25
Category : Science
ISBN : 9781439862384

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Dynamic Force Spectroscopy and Biomolecular Recognition by Anna Rita Bizzarri,Salvatore Cannistraro Pdf

Molecular recognition, also known as biorecognition, is the heart of all biological interactions. Originating from protein stretching experiments, dynamic force spectroscopy (DFS) allows for the extraction of detailed information on the unbinding process of biomolecular complexes. It is becoming progressively more important in biochemical studies and is finding wider applications in areas such as biophysics and polymer science. In six chapters, Dynamic Force Spectroscopy and Biomolecular Recognition covers the most recent ideas and advances in the field of DFS applied to biorecognition: Chapter 1: Reviews the basic and novel aspects of biorecognition and discusses the emerging capabilities of single-molecule techniques to disclose kinetic properties and molecular mechanisms usually hidden in bulk measurements Chapter 2: Describes the basic principle of atomic force microsocopy (AFM) and DFS, with particular attention to instrumental and theoretical aspects more strictly related to the study of biomolecules Chapter 3: Overviews the theoretical background in which experimental data taken in nonequilibrum measurements of biomolecular unbinding forces are extrapolated to equilibrium conditions Chapter 4: Reviews the most common and efficient strategies adopted in DFS experiments to immobilize the interacting biomolecules to the AFM tip and to the substrate Chapter 5: Presents and discusses the most representative aspects related to the analysis of DFS data and the challenges of integrating well-defined criteria to calibrate data in automatic routinary procedures Chapter 6: Overviews the most relevant DFS applications to study biorecognition processes, including the biotin/avidin pair, and selected results on various biological complexes, including antigen/antibody, proteins/DNA, and complexes involved in adhesion processes Chapter 7: Summarizes the main results obtained by DFS applied to study biorecognition processes with forthcoming theoretical and experimental advances Although DFS is a widespread, worldwide technique, no books focused on this subject have been available until now. Dynamic Force Spectroscopy and Biomolecular Recognition provides the state of the art of experimental data analysis and theoretical procedures, making it a useful tool for researchers applying DFS to study biorecognition processes.

Author : Anonim
Publisher : Walter de Gruyter
Page : 452 pages
File Size : 52,7 Mb
Release : 2013-08-01
Category : Science
ISBN : 9783110298345

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Complex Flavoproteins, Dehydrogenases and Physical Methods by Anonim Pdf

The dynamic field of flavin and flavoprotein biochemistry has seen rapid advancement in recent years. This comprehensive two volume set provides an overview of all aspects of contemporary research in this important class of enzymes. Topics treated include flavoproteins involved in energy generation, signal transduction and electron transfer (including respiration); oxygen activation by flavoproteins; the biology and biochemistry of complex flavoproteins; flavin and flavoprotein photochemistry/photophysics as well as biotechnological applications of flavoproteins. Recent developments in this field include new structures (including those of large membrane-integral electron transfer complexes containing FMN or FAD), elucidation of the role of flavoproteins in cell signalling pathways (including both phototaxis and the circadian cycle) and important new insights into the reaction mechanisms of flavin-containing enzymes. This volume focusing on complex flavoproteins and physical methods is an essential reference for all researchers in biochemistry, chemistry, photochemistry and photophysics working on flavoenzymes.

Author : Aleksandr Noy
Publisher : Springer Science & Business Media
Page : 300 pages
File Size : 53,7 Mb
Release : 2007-12-03
Category : Science
ISBN : 9780387499895

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Handbook of Molecular Force Spectroscopy by Aleksandr Noy Pdf

Researchers in academia and industry who are interested in techniques for measuring intermolecular forces will find this an essential text. It presents a review of modern force spectroscopy, including fundamentals of intermolecular forces, technical aspects of the force measurements, and practical applications. The handbook begins with a review of the fundamental physics of loading single and multiple chemical bonds on the nanometer scale. It contains a discussion of thermodynamic and kinetic models of binding forces and dissipation effects in nanoscale molecular contacts, covers practical aspects of modern single-molecule level techniques, and concludes with applications of force spectroscopy to chemical and biological processes. Computer modeling of force spectroscopy experiments is also addressed.

Author : Laura T. Mazzola
Publisher : Unknown
Page : 264 pages
File Size : 51,8 Mb
Release : 1999
Category : Electronic
ISBN : STANFORD:36105023670198

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Probing Biomolecular Recognition Using Atomic Force Microscopy by Laura T. Mazzola Pdf

Author : Nuno C. Santos,Filomena A. Carvalho
Publisher : Unknown
Page : 372 pages
File Size : 45,9 Mb
Release : 2018
Category : Atomic force microscopy
ISBN : 1493988948

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Atomic Force Microscopy by Nuno C. Santos,Filomena A. Carvalho Pdf

Author : Malgorzata Lekka
Publisher : CRC Press
Page : 228 pages
File Size : 44,5 Mb
Release : 2017-07-06
Category : Medical
ISBN : 9789814669689

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Cellular Analysis by Atomic Force Microscopy by Malgorzata Lekka Pdf

Despite substantial evidence showing the feasibility of Atomic Force Microscopy (AFM) to identify cells with altered elastic and adhesive properties, the use of this technique as a complementary diagnostic method remains controversial. This book is designed to be a practical textbook that teaches how to assess the mechanical characteristics of living, individual cells by AFM. Following a step-by-step approach, it introduces the methodology of measurements in the case of both determination of elastic properties and quantification of adhesive properties.

Author : Anne-Sophie Duwez,Nicolas Willet
Publisher : CRC Press
Page : 291 pages
File Size : 54,9 Mb
Release : 2011-12-07
Category : Science
ISBN : 9781439809662

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Molecular Manipulation with Atomic Force Microscopy by Anne-Sophie Duwez,Nicolas Willet Pdf

With the invention of scanning probe techniques in the early 1980s, scientists can now play with single atoms, single molecules, and even single bonds. Force, dynamics, and function can now be probed at the single-molecule level. Molecular Manipulation with Atomic Force Microscopy (AFM) presents a series of topics that discuss concepts and methodologies used to manipulate and study single (bio)molecules with AFM. The first part is dedicated to the pulling of single molecules with force spectroscopy to investigate molecular interactions, mechanics, and mechanochemical processes, and the second part to the manipulation, repositioning, and targeted delivery of single molecules on substrates. Single molecule manipulation is an exciting area of research which made important breakthroughs in nanoscience and which could find potential applications in a diverse range of disciplines, including chemistry, biology, physics, material and polymer science, and engineering. New and experienced AFM researchers looking for applications beyond imaging will find a wealth of information in this informative volume.

Author : Bharat Bhushan
Publisher : Springer Science & Business Media
Page : 1964 pages
File Size : 40,5 Mb
Release : 2010-04-23
Category : Technology & Engineering
ISBN : 9783642025259

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Springer Handbook of Nanotechnology by Bharat Bhushan Pdf

Since 2004 and with the 2nd edition in 2006, the Springer Handbook of Nanotechnology has established itself as the definitive reference in the nanoscience and nanotechnology area. It integrates the knowledge from nanofabrication, nanodevices, nanomechanics, Nanotribology, materials science, and reliability engineering in just one volume. Beside the presentation of nanostructures, micro/nanofabrication, and micro/nanodevices, special emphasis is on scanning probe microscopy, nanotribology and nanomechanics, molecularly thick films, industrial applications and microdevice reliability, and on social aspects. In its 3rd edition, the book grew from 8 to 9 parts now including a part with chapters on biomimetics. More information is added to such fields as bionanotechnology, nanorobotics, and (bio)MEMS/NEMS, bio/nanotribology and bio/nanomechanics. The book is organized by an experienced editor with a universal knowledge and written by an international team of over 150 distinguished experts. It addresses mechanical and electrical engineers, materials scientists, physicists and chemists who work either in the nano area or in a field that is or will be influenced by this new key technology.

Author : P. Carmona,R. Navarro,A. Hernanz
Publisher : Springer Science & Business Media
Page : 661 pages
File Size : 49,5 Mb
Release : 2012-12-06
Category : Science
ISBN : 9789401156226

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Spectroscopy of Biological Molecules: Modern Trends by P. Carmona,R. Navarro,A. Hernanz Pdf

The 1997 European Conference on Spectroscopy of Biological Molecules (ECSBM) is the seventh in a biennial series of conferences devoted to the applications of molecular spectroscopy to biological molecules and related systems. The interest of these conferences rests mainly on the relationship between the structure and physiological activity of biological molecules and related systems of which these molecular species form part. This volume ofECSBM contains articles prepared by the invited lecturers and those making poster presentations at the seventh ECSBM. The reader will find mainly applications of vibrational spectroscopy to protein structure and dynamics, biomembranes, molecular recognition, nucleic acids and other biomolecules and biological systems containing specific chromophors. Biomedical applications of vibrational spectroscopy are expanding rapidly. On the other hand, a significant number of the papers describe applications of other methods, such as NMR, circular dichroism, optical absorption and fluorescence, X-ray absorption and diffraction and other theoretical methods. One aim has been to achieve a well balanced, critically comparative review of recent progress in the field of biomolecular structure, bonding and dynamics based on applications of the above spectroscopic methods. A great part of the contributions included in this volume are devoted to biomedical and biotechnological applications and provide a broadly based account of recent applicationS in this field. The content of this book has been organized in sections corresponding mainly to the different types of biological molecules investigated. This book includes also another section related to theoretical methods where MO calculations of vibrational frequencies dominate clearly the topic.

Author : Christof M. Niemeyer,Chad A. Mirkin
Publisher : John Wiley & Sons
Page : 491 pages
File Size : 46,9 Mb
Release : 2006-03-06
Category : Science
ISBN : 9783527605910

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Nanobiotechnology by Christof M. Niemeyer,Chad A. Mirkin Pdf

Nanotechnology is the key technology of the 21st century. The possibility to exploit the structures and processes of biomolecules for novel functional materials, biosensors, bioelectronics and medical applications has created the rapidly growing field of nanobiotechnology. Designed as a broad survey of the field, this book combines contributions from bioorganic and bioinorganic chemistry, molecular biology, materials science and bioanalytics to fathom the full scope of current and future developments. It is divided into four main sections: * Interphase Systems * Protein-based Nanostructures * DNA-based Nanostructures * Nanoanalytics Each chapter describes in detail currently available methods and contains numerous references to the primary literature, making this the perfect "field guide" for chemists, biologists and materials scientists who want to explore the fascinating world of nanobiotechnology.

Author : Jiye Cai
Publisher : Springer
Page : 235 pages
File Size : 45,8 Mb
Release : 2018-11-03
Category : Science
ISBN : 9789811315107

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Atomic Force Microscopy in Molecular and Cell Biology by Jiye Cai Pdf

The book addresses new achievements in AFM instruments – e.g. higher speed and higher resolution – and how AFM is being combined with other new methods like NSOM, STED, STORM, PALM, and Raman. This book explores the latest advances in atomic force microscopy and related techniques in molecular and cell biology. Atomic force microscopy (AFM) can be used to detect the superstructures of the cell membrane, cell morphology, cell skeletons and their mechanical properties. Opening up new fields of in-situ dynamic study for living cells, enzymatic reactions, fibril growth and biomedical research, these combined techniques will yield valuable new insights into molecule and cell biology. This book offers a valuable resource for students and researchers in the fields of biochemistry, cell research and chemistry etc.

Author : Anirban Bhunia,Hanudatta S Atreya,Neeraj Sinha
Publisher : Royal Society of Chemistry
Page : 733 pages
File Size : 41,7 Mb
Release : 2022-08-17
Category : Science
ISBN : 9781839162091

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NMR Spectroscopy for Probing Functional Dynamics at Biological Interfaces by Anirban Bhunia,Hanudatta S Atreya,Neeraj Sinha Pdf

Providing a comprehensive amalgamation of the scattered knowledge of how to apply high-resolution NMR techniques to biomolecular systems, this book will break down the conventional stereotypes in the use of NMR for structural studies.

Author : Rudolf Merkel
Publisher : Unknown
Page : 43 pages
File Size : 48,8 Mb
Release : 2001
Category : Electronic
ISBN : OCLC:264800153

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Force Spectroscopy on Single Passive Biomolecules and Single Biomolecular Bonds by Rudolf Merkel Pdf

Author : Milad Radiom
Publisher : Springer
Page : 117 pages
File Size : 48,7 Mb
Release : 2015-03-30
Category : Science
ISBN : 9783319140483

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Correlation Force Spectroscopy for Single Molecule Measurements by Milad Radiom Pdf

This thesis addresses the development of a new force spectroscopy tool, correlation force spectroscopy (CFS) for the measurement of the properties of very small volumes of material (molecular to μm3) at kHz-MHz frequency range. CFS measures the simultaneous thermal fluctuations of two closely-spaced atomic force microscopy (AFM) cantilevers. CFS then calculates the cross-correlation in the thermal fluctuations that gives the mechanical properties of the matter that spans the gap of the two cantilevers. The book also discusses development of CFS, its advantages over AFM, and its application in single molecule force spectroscopy and micro-rheology.

Author : Anonim
Publisher : Elsevier
Page : 352 pages
File Size : 48,9 Mb
Release : 2013-09-04
Category : Science
ISBN : 9780124165977

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Biomolecular Spectroscopy: Advances from Integrating Experiments and Theory by Anonim Pdf

Published continuously since 1944, Advances in Protein Chemistry and Structural Biology has been a continuous, essential resource for protein chemists. Covering reviews of methodology and research in all aspects of protein chemistry, including purification/expression, proteomics, modeling and structural determination and design, each volume brings forth new information about protocols and analysis of proteins while presenting the most recent findings from leading experts in a broad range of protein-related topics. Covers reviews of methodology and research in all aspects of protein chemistry Brings forth new information about protocols and analysis of proteins while presenting the most recent findings from leading experts in a broad range of protein-related topics