Electron Microscopy Of Plant Pathogens

Author : Kurt Mendgen,Dietrich-Eckhardt Lesemann
Publisher : Springer Science & Business Media
Page : 342 pages
File Size : 54,8 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642758188

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Electron Microscopy of Plant Pathogens by Kurt Mendgen,Dietrich-Eckhardt Lesemann Pdf

Plants, fungi, and viruses were among the first biological objects studied with an electron microscope. One of the two first instruments built by Siemens was used by Helmut Ruska, a brother of Ernst Ruska, the pioneer in constructing electron microscopes. H. Ruska published numerous papers on different biological objects in 1939. In one of these, the pictures by G. A. Kausche, E. Pfankuch, and H. Ruska of tobacco mosaic virus opened a new age in microscopy. The main problem was then as it still is today, to obtain an appropriate preparation of the specimen for observation in the electron microscope. Beam damage and specimen thickness were the first obstacles to be met. L. Marton in Brussels not only built his own instrument, but also made considerable progress in specimen preparation by introducing the impregnation of samples with heavy metals to obtain useful contrast. His pictures of the bird nest orchid root impregnated with osmium were revolutionary when published in 1934. It is not the place here to recall the different techniques which were developed in the subsequent years to attain the modern knowledge on the fine structure of plant cells and of different plant pathogens. The tremendous progress obtained with tobacco mosaic virus is reflected in the chapter by M. Wurtz on the fine structure of viruses in this Volume. New cytochemical and immunological techniques considerably surpass the morphological information obtained from the pathogens, especially at the host-parasite interface.

Author : Kurt Mendgen,Dietrich-Eckhardt Lesemann
Publisher : Springer
Page : 336 pages
File Size : 47,9 Mb
Release : 1990-12-10
Category : Science
ISBN : 354052777X

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Electron Microscopy of Plant Pathogens by Kurt Mendgen,Dietrich-Eckhardt Lesemann Pdf

Plants, fungi, and viruses were among the first biological objects studied with an electron microscope. One of the two first instruments built by Siemens was used by Helmut Ruska, a brother of Ernst Ruska, the pioneer in constructing electron microscopes. H. Ruska published numerous papers on different biological objects in 1939. In one of these, the pictures by G. A. Kausche, E. Pfankuch, and H. Ruska of tobacco mosaic virus opened a new age in microscopy. The main problem was then as it still is today, to obtain an appropriate preparation of the specimen for observation in the electron microscope. Beam damage and specimen thickness were the first obstacles to be met. L. Marton in Brussels not only built his own instrument, but also made considerable progress in specimen preparation by introducing the impregnation of samples with heavy metals to obtain useful contrast. His pictures of the bird nest orchid root impregnated with osmium were revolutionary when published in 1934. It is not the place here to recall the different techniques which were developed in the subsequent years to attain the modern knowledge on the fine structure of plant cells and of different plant pathogens. The tremendous progress obtained with tobacco mosaic virus is reflected in the chapter by M. Wurtz on the fine structure of viruses in this Volume. New cytochemical and immunological techniques considerably surpass the morphological information obtained from the pathogens, especially at the host-parasite interface.

Author : P. Narayanasamy
Publisher : CRC Press
Page : 548 pages
File Size : 44,5 Mb
Release : 2001-08-28
Category : Science
ISBN : 0824705912

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Plant Pathogen Detection and Disease Diagnosis, Second Edition, by P. Narayanasamy Pdf

This work provides information on the detection, identification, and differentiation of all microbial plant pathogens - presenting modern protocols for rapid diagnosis of diseases based on biological, physical, chemical and molecular properties. It contains methods for the selection of disease-free seeds and vegetatively propagated planting materials and quarantine techniques for screening newly introduced plant materials.

Author : U. S. Singh,Rudra P. Singh
Publisher : CRC Press
Page : 836 pages
File Size : 49,6 Mb
Release : 2017-12-14
Category : Science
ISBN : 9781351430210

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Molecular Methods in Plant Pathology by U. S. Singh,Rudra P. Singh Pdf

Molecular Methods in Plant Pathology covers methods in phytopathology at the molecular level, including PCR techniques, electron microscopy, tissue culturing, and the cloning of disease-resistant genes. Phytopathologists, botanists, horticulturists, and anyone working in agriculture will find this a useful reference on biophysical, biochemical, biomolecular, and biotechnological methods.

Author : A. B. A. M. Baudoin,Gerald Ray Hooper,Don E. Mathre,R. B. Carroll
Publisher : Amer Phytopathological Society
Page : 196 pages
File Size : 46,8 Mb
Release : 1988
Category : Science
ISBN : OCLC:503610251

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Laboratory Exercises in Plant Pathology by A. B. A. M. Baudoin,Gerald Ray Hooper,Don E. Mathre,R. B. Carroll Pdf

How to use this kit; Use and care of the light microscope; Recognition and terminology of disease symptoms and signs; Isolation of fungi and bacteria; Diagnosis of disease and proof of pathogenicity; Plant disease diagnosis in practice; Characteristics and structures of fungi; Root infection by phytophtora; Damping-off of seeds and seedlings; Soils fungistasis; Pathogen survival in soil; Biological control of soilborne plant pathogens by antagonistic fungi; Soilborne fungi; Population assessment of fusarium spp; Foliar infection by fungi; Dissemination of fungus spores and bacterial cells by wind and water; Fungicides for the control of foliar infection; Race identification of wheat leaf rust; Bacterial pathogens; Crown gall; Frost injury and ice nucleating bacteria; Viruses: mechanical transmission and host range; Insect transmission of plant viruses; Electron microscopy; Virus identification by serology; Study of plant-parasitic nematodes; Root-Knot nematodes: Symptoms, life stages, and control; Cyst nematodes; Foliar nematodes on begonia: Physiological spects of plant disease; Abiotic diseases; Disorders caused by higher plant.

Author : Anthony William Robards
Publisher : Unknown
Page : 324 pages
File Size : 43,5 Mb
Release : 1970
Category : Science
ISBN : MINN:31951000083839R

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Electron Microscopy and Plant Ultrastructure by Anthony William Robards Pdf

Author : R. T. V. Fox
Publisher : Oxford University Press, USA
Page : 236 pages
File Size : 44,6 Mb
Release : 1993
Category : Art
ISBN : UOM:39015032834684

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Principles of Diagnostic Techniques in Plant Pathology by R. T. V. Fox Pdf

The correct diagnosis of a plant disease is an essential prerequisite of its successful control. Diagnostic methods today include a number of traditional techniques such as direct observation or microscopy, as well as more recently developed procedures such as those based on immunological or nucleic acid analysis. This book provides a text reviewing the principles of all these techniques that will be suitable for advanced students of plant pathology who already have some basic background in the subject. The theories behind the methods are described and illustrated with numerous examples of plant diseases caused by fungi, bacteria and viruses, and the strengths and limitations of different techniques are compared. The book includes a number of colour photographs and will provide a very useful overview of this rapidly developing subject.

Author : R. E. F. Matthews
Publisher : CRC Press
Page : 391 pages
File Size : 49,7 Mb
Release : 2017-12-08
Category : Electronic
ISBN : 1315892251

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Diagnosis of Plant Virus Diseases by R. E. F. Matthews Pdf

Diagnosis of Plant Virus Diseases presents a comprehensive summary of methods currently available for the diagnosis of plant diseases caused by viruses and viroids. Up-to-date literature references are provided, brief accounts of the basis for particular methods are included, and detailed protocols are presented. Procedures discussed include the use of host plants, electron microscopy of in vitro preparations, serological procedures (especially forms of ELISA, monoclonal antibodies, serological specific electron microscopy, and immunoblotting), and nucleic acid hybridization procedures. Strategies are outlined for implicating virus-like pathogens as causes of diseases of unknown etiology, and problems involved in identifying complexes of transmission-dependent and helper viruses are discussed. The book will be extremely useful for phytopathologists, plant virologists, and research students and workers in plant virology laboratories and diagnostic plant pathology laboratories.

Author : Iftikhar Ahmad
Publisher : Unknown
Page : 100 pages
File Size : 53,8 Mb
Release : 1992
Category : Plant diseases
ISBN : CORNELL:31924068968217

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Phytopathological Diagnostic Techniques by Iftikhar Ahmad Pdf

Author : Eleanor Elmendorf
Publisher : Unknown
Page : 130 pages
File Size : 47,6 Mb
Release : 1977
Category : Phytopathogenic microorganisms
ISBN : WISC:89119925626

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Newly Discovered Prokaryotic Plant Pathogens by Eleanor Elmendorf Pdf

Author : Holger B. Deising
Publisher : Springer Science & Business Media
Page : 408 pages
File Size : 47,6 Mb
Release : 2009-02-07
Category : Science
ISBN : 9783540874072

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Plant Relationships by Holger B. Deising Pdf

Since the publication of the first edition of "The Mycota Vol. V – Plant Relationships" in 1997, tremendous advances in fungal molecular biology and biochemistry have taken place; and both light and electron microscopical techniques have improved considerably. These new insights led to a better understanding of the relationships between fungi and plants; and a completely revised new edition of Plant Relationships could be produced, providing an up-to-date overview on mutualistic and pathogenic interactions. In 18 chapters internationally acknowledged authors present reviews on fungal lifestyles, mechanisms of their interactions with their host plants, signal perception and transduction, and plant defense responses directed against attack by fungal pathogens. Highlighting the recent developments in fungus-plant interactions, this volume is indispensable for researchers, lecturers and students in microbiology, mycology and plant sciences, including plant pathology.

Author : Sarah Jane Gurr,M. J. McPherson,Dianna J. Bowles
Publisher : Irl Press
Page : 568 pages
File Size : 48,6 Mb
Release : 1992-10
Category : Molecular microbiology
ISBN : 0199633533

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Molecular Plant Pathology by Sarah Jane Gurr,M. J. McPherson,Dianna J. Bowles Pdf

V.1: Pathogens and pathogen manipulation; Geminiviruses (wheat dwarf virus); RNA-based viruses; Bacteria; Introduction of cloned DNA into plant pathogenic bacteria; RFLP analyses and gene tagging for bacterial identification and taxonomy; Fungi; Gene transformation in plant pathogenic fungi; Nematodes; Nucleic acid isolation and hybridization techniques; The polymerase chain reaction; PCR; Analysis of defence gene transcriptional regulation; In situ hybridization in plants; Gene expression using baculoviruses. v.2: Callose and callose synthase; Lignin and associated phenolic acids in cell walls; preparation and analysis of intercellular fluid; Biochemical analysis of chitinases and glucanases; Localization of proteins and carbohydrates using immunogold labelling in light and electron microscopy; Isoflavonoid phytoalexins and their biosynthetic enzymes; Analysis of terpenoid phytoalexins and their biosynthetic enzymes; Preparation and characterization of oligosaccharide elicitors of phytoalexin accumulation; Xyloglucan oligosaccharides; Analysis of calcium involvement in host-pathogen interactions; Analysis of components of the plant phosphoinositide system; Metabolic changes following infection of leaves by fungi and viruses; Strategies for cloning plant disease resistance genes.

Author : R. E. F. Matthews
Publisher : CRC Press
Page : 392 pages
File Size : 51,9 Mb
Release : 2019-07-23
Category : Science
ISBN : 9781351079808

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Diagnosis of Plant Virus Diseases by R. E. F. Matthews Pdf

Diagnosis of Plant Virus Diseases presents a comprehensive summary of methods currently available for the diagnosis of plant diseases caused by viruses and viroids. Up-to-date literature references are provided, brief accounts of the basis for particular methods are included, and detailed protocols are presented. Procedures discussed include the use of host plants, electron microscopy of in vitro preparations, serological procedures (especially forms of ELISA, monoclonal antibodies, serological specific electron microscopy, and immunoblotting), and nucleic acid hybridization procedures. Strategies are outlined for implicating virus-like pathogens as causes of diseases of unknown etiology, and problems involved in identifying complexes of transmission-dependent and helper viruses are discussed. The book will be extremely useful for phytopathologists, plant virologists, and research students and workers in plant virology laboratories and diagnostic plant pathology laboratories.

Author : Perumal Narayanasamy
Publisher : John Wiley & Sons
Page : 1176 pages
File Size : 47,6 Mb
Release : 2016-12-08
Category : Technology & Engineering
ISBN : 9781119195788

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Microbial Plant Pathogens by Perumal Narayanasamy Pdf

Healthy seeds and propagules are the basic requirement for producing good grains, fruits and vegetables needed for human survival and perpetuation. Dispersal of microbial plant pathogens via seeds and propagules has assumed more importance than other modes of dispersal, as infected seeds and propagules have the potential to become the primary sources of carrying pathogen inoculum for subsequent crops. Several diseases transmitted through seeds and propagules have been shown to have the potential to damage economies as a result of huge quantitative and qualitative losses in numerous crops. Hence, it is essential to rapidly detect, identify and differentiate the microbial plant pathogens present in seeds and propagules precisely and reliably, using sensitive techniques. Microbial Plant Pathogens: Detection and Management in Seeds and Propagules provides a comprehensive resource on seed-borne and propagule-borne pathogens. Information on the biology of microbial pathogens, including genetic diversity, infection process and survival mechanisms of pathogens and epidemiology of diseases caused by them, are discussed critically and in detail to highlight weak links in the life cycles of the pathogens. Development of effective disease management systems, based on the principles of exclusion and eradication of pathogens and immunization of crop plants to enhance the levels of resistance of cultivars to diseases, has been effective to keep the pathogens at bay. The need for production of disease-free seeds/propagules has been emphasized to prevent the carryover of the inoculum to the next crop or introduction of the pathogens to other locations. Effectiveness of adopting simple cultural practices and development of cultivars resistant to diseases through traditional breeding methods or biotechnological approach have resulted in reducing the pathogen inoculum and disease incidence. Although application of different chemicals may reduce the disease incidence effectively, biological management of crop diseases, employing potential biological control agents have to be preferred to preserve the agroecosystems. Greater efforts have to be made to integrate compatible strategies to enhance the effectiveness of diseases management systems. Protocols appended at the end of relevant chapters form a unique feature of this book to enable the researchers to fine-tune their projects. This 2 volume set provides comprehensive and updated information about the economically-important groups of microbial plant pathogens carried by seed and propagules. Graduate students, researchers and teachers of plant pathology, plant protection, microbiology, plant breeding and genetics, agriculture and horticulture, as well as certification and quarantine personnel will find the information presented in this book useful.

Author : P. Narayanasamy
Publisher : Springer Science & Business Media
Page : 277 pages
File Size : 52,8 Mb
Release : 2010-10-20
Category : Science
ISBN : 9789048197699

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Microbial Plant Pathogens-Detection and Disease Diagnosis: by P. Narayanasamy Pdf

Microbial plant pathogens causing qualitative and quantitative losses in all corps are present not only in the infected plants, but also in the environmental comprising of soil, water and air. The vectors present in the environment spread the microbial pathogens to short and/or long distances. Detection of microbial pathogens rapidly and reliably by employing suitable sensitive applicable for different ecosystems. The pathogens have to be identified precisely and differentiated and quantified to plan appropriate short- and long-term strategies to contain the incidence and spread of diseases induced by them. This book aims to present all relevant and latest information on the detection techniques based on the biological, biochemical, immunological and nucleic acid characteristics of microbial pathogens presents in the host plants, as well as in the natural substrates that support the survival and perpetuation of the pathogens.