Enzyme Assays

Author : Robert Eisenthal,Michael J. Danson
Publisher : Practical Approach
Page : 310 pages
File Size : 46,9 Mb
Release : 2002
Category : Analysis
ISBN : 0199638209

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Enzyme Assays by Robert Eisenthal,Michael J. Danson Pdf

Enzyme assays are among the most frequently performed procedures in biochemistry and are routinely used to estimate the amount of enzyme present in a cell or tissue, to follow the purification of an enzyme, or to determine the kinetic parameters of a system. The range of techniques used tomeasure the rate of an enzyme-catalysed reaction is limited only by the nature of the chemical change and the ingenuity of the investigator. This book describes the design and execution of enzyme assays, covering both general principles and specific chapters.Building upon the highly popular first edition, this book combines revised or rewritten chapters with entirely new contributions. Topics include experimental protocols covering photometric, radiometric, HPLC, and electrochemical assays, along with methods for determining enzyme assays after gelelectrophoresis. The theory underlying each method is outlined, together with a description of the instrumentation, sensitivity and sources of error. Also included are chapters on the principles of enzyme assay and kinetic studies; techniques for enzyme extraction; high- throughout screening;statistical analysis of enzyme kinetic data; and the determination of active site concentration.This second edition of Enzyme Assays will be valuable not only to biochemists, but to researchers in all areas of the life sciences.

Author : Jean-Louis Reymond
Publisher : John Wiley & Sons
Page : 386 pages
File Size : 49,6 Mb
Release : 2006-05-12
Category : Science
ISBN : 9783527607211

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Enzyme Assays by Jean-Louis Reymond Pdf

Edited by one of the leading experts in the field, this book fills the need for a book presenting the most important methods for high-throughput screenings and functional characterization of enzymes. It adopts an interdisciplinary approach, making it indispensable for all those involved in this expanding field, and reflects the major advances made over the past few years. For biochemists, analytical, organic and catalytic chemists, and biotechnologists.

Author : Hans Bisswanger
Publisher : John Wiley & Sons
Page : 533 pages
File Size : 51,8 Mb
Release : 2019-11-06
Category : Science
ISBN : 9783527820801

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Practical Enzymology by Hans Bisswanger Pdf

A practice-oriented guide to assaying more than 100 of the most important enzymes, complete with the theoretical background and specific protocols for immediate use in the biochemical laboratory. Now expanded with a new section on metal ion determination.

Author : Donald Bernard Roodyn
Publisher : Elsevier
Page : 225 pages
File Size : 40,5 Mb
Release : 1970
Category : Electronic
ISBN : 9780720442069

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Automated Enzyme Assays by Donald Bernard Roodyn Pdf

Author : Samira Hosseini,Patricia Vázquez-Villegas,Marco Rito-Palomares,Sergio O. Martinez-Chapa
Publisher : Springer
Page : 115 pages
File Size : 47,7 Mb
Release : 2017-12-30
Category : Technology & Engineering
ISBN : 9789811067662

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Enzyme-linked Immunosorbent Assay (ELISA) by Samira Hosseini,Patricia Vázquez-Villegas,Marco Rito-Palomares,Sergio O. Martinez-Chapa Pdf

This book offers comprehensive information on all aspects of ELISA, starting with the fundamentals of the immune system. It also reviews the history of analytical assays prior to the advent of ELISA (enzyme-linked immunosorbent assay) and addresses the materials of choice for the fabrication of the platforms, possible biomolecular interactions, different protocols, and evaluation parameters. The book guides readers through the respective steps of the analytical assay, while also familiarizing them with the possible sources of error in the assay. It offers detailed insights into the immobilization techniques used for protein attachment, as well as methods for evaluating the assay and calculating the key parameters, such as sensitivity, specificity, accuracy and limit of detection. In addition, the book explores the advantages and shortcomings of the conventional ELISA, as well as various approaches to improving its performance. In this regard, merging and integrating other technologies with widely known ELISAs have opened new avenues for the advancement of this immunoassay. Accordingly, the book provides cutting-edge information on integrated platforms such as ELISpot, plasmonic ELISAs, sphere-/bead-based ELISAs, paper-/fiber-based ELISAs and ELISA in micro-devices.

Author : Abhilasha Singh
Publisher : Daya Books
Page : 1096 pages
File Size : 45,9 Mb
Release : 2007
Category : Enzymes
ISBN : 8189233491

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Enzyme Assays by Abhilasha Singh Pdf

Author : Richard P. Dick
Publisher : John Wiley & Sons
Page : 416 pages
File Size : 41,5 Mb
Release : 2020-01-22
Category : Technology & Engineering
ISBN : 9780891188544

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Methods of Soil Enzymology by Richard P. Dick Pdf

Methods of Soil Enzymology provides the first comprehensive set of vetted methods for studying enzymes in soils. Readers will especially benefit from the step-by-step explanation of the lab procedures, as well as background information for using these methods effectively and analyzing data. Main topics include activity assays, enzyme extraction, and synthetic enzyme complexes. Each method covered includes background informaton, step-by-step descriptions of the procedure, and special comments regarding nuances, pitfalls, and interpretation of the method. Learn the latest research methods, including enzyme extraction methods and procedures for creating synthetic enzyme complexes, as well as the newest ways to use small-scale and high-throughput methods for enzyme activity assays. Written for the researcher, but welcoming to those new to soil enzymology, the introduction includes conceptual information to orient those who are not familiar with these methods but want to use them. In the tradition of SSSA methods books, Methods of Soil Enzymology features a comprehensive approach with a focus on ease of use.

Author : Edward F. Rossomando
Publisher : John Wiley & Sons
Page : 480 pages
File Size : 43,9 Mb
Release : 2009-09-25
Category : Science
ISBN : 9780470111000

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HPLC in Enzymatic Analysis by Edward F. Rossomando Pdf

The use of High Performance Liquid Chromatography (HPLC) techniques in the study of enzymatic reactions has grown significantly since the publication of the first edition of this highly successful book: the role of enzymes in biological research has expanded; the application of HPLC and enzymes has extended to more disciplines; advances in separation techniques and instrumentation have increased the capability of HPLC; and the discovery of new enzymes has spawned new methods of analysis. High Performance Liquid Chromatography in Enzymatic Analysis, Second Edition addresses these developments in its coverage of the refinements of HPLC methods and their use in a wide range of laboratory applications. It offers the same practical approach found in the first edition, incorporates a wealth of new information into existing chapters, and adds new chapters to deal with new applications, including capillary electrophoresis, forensic chemistry, microdialysis, and the polymerase chain reaction. Topics include: * Application of HPLC to the assay of enzymatic activities * Concepts and principles of HPLC, including the latest technological advances * Concepts and principles of capillary electrophoresis (CE) * Strategy for design of an HPLC/CE system for assay of enzyme activity * Preparation of enzymatic activities from tissues and single cells * Analysis of enzymatic activities in body fluids, including chromatobiosis * HPLC for the identification of new enzymatic activities * Fundamentals of the polymerase chain reaction * HPLC in forensics * Survey of enzymatic activities assayed by the HPLC method, including many new categories * Multienzyme systems, including many new examples * HPLC in the analysis of contaminated food "It is the ability of HPLC to accomplish separations completely and rapidly that led to its original application to problems in the life sciences, particularly those related to purification. An analysis of the literature revealed that this technique was used primarily for the purification of small molecules, macromolecules such as peptides and proteins, and more recently, antibodies. This application to purification has all but dominated the use of the method, and there has been a plethora of books, symposia, and conferences on the use of HPLC for these purposes. However, it was only a matter of time before others began to look beyond and to explore the possibilities that result from the capacity to make separations quickly and efficiently." --from the preface to the First Edition Easy to read and full of practical advice and hundreds of diagrams and examples, High Performance Liquid Chromatography in Enzymatic Analysis, Second Edition is an invaluable resource for students, researchers, and laboratory workers in analytical chemistry and biochemistry, molecular biology and cell biology, and for anyone interested in keeping up with this fast-growing field.

Author : N.S. Punekar
Publisher : Springer
Page : 562 pages
File Size : 52,5 Mb
Release : 2018-11-11
Category : Science
ISBN : 9789811307850

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ENZYMES: Catalysis, Kinetics and Mechanisms by N.S. Punekar Pdf

This enzymology textbook for graduate and advanced undergraduate students covers the syllabi of most universities where this subject is regularly taught. It focuses on the synchrony between the two broad mechanistic facets of enzymology: the chemical and the kinetic, and also highlights the synergy between enzyme structure and mechanism. Designed for self-study, it explains how to plan enzyme experiments and subsequently analyze the data collected. The book is divided into five major sections: 1] Introduction to enzymes, 2] Practical aspects, 3] Kinetic Mechanisms, 4] Chemical Mechanisms, and 5] Enzymology Frontiers. Individual concepts are treated as stand-alone chapters; readers can explore any single concept with minimal cross-referencing to the rest of the book. Further, complex approaches requiring specialized techniques and involved experimentation (beyond the reach of an average laboratory) are covered in theory with suitable references to guide readers. The book provides students, researchers and academics in the broad area of biology with a sound theoretical and practical knowledge of enzymes. It also caters to those who do not have a practicing enzymologist to teach them the subject.

Author : Hans Bisswanger
Publisher : John Wiley & Sons
Page : 419 pages
File Size : 54,5 Mb
Release : 2019-10-21
Category : Science
ISBN : 9783527346042

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Practical Enzymology by Hans Bisswanger Pdf

A practice-oriented guide to assaying more than 100 of the most important enzymes, complete with the theoretical background and specific protocols for immediate use in the biochemical laboratory. Now expanded with a new section on metal ion determination.

Author : Robert A. Copeland
Publisher : John Wiley & Sons
Page : 416 pages
File Size : 43,8 Mb
Release : 2004-04-07
Category : Science
ISBN : 9780471461852

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Enzymes by Robert A. Copeland Pdf

Fully updated and expanded-a solid foundation for understandingexperimental enzymology. This practical, up-to-date survey is designed for a broadspectrum of biological and chemical scientists who are beginning todelve into modern enzymology. Enzymes, Second Editionexplains the structural complexities of proteins and enzymes andthe mechanisms by which enzymes perform their catalytic functions.The book provides illustrative examples from the contemporaryliterature to guide the reader through concepts and data analysisprocedures. Clear, well-written descriptions simplify the complexmathematical treatment of enzyme kinetic data, and numerouscitations at the end of each chapter enable the reader to accessthe primary literature and more in-depth treatments of specifictopics. This Second Edition of Enzymes: A Practical Introductionto Structure, Mechanism, and Data Analysis features refinedand expanded coverage of many concepts, while retaining theintroductory nature of the book. Important new featuresinclude: A new chapter on protein-ligand binding equilibria Expanded coverage of chemical mechanisms in enzyme catalysisand experimental measurements of enzyme activity Updated and refined discussions of enzyme inhibitors andmultiple substrate reactions Coverage of current practical applications to the study ofenzymology Supplemented with appendices providing contact information forsuppliers of reagents and equipment for enzyme studies, as well asa survey of useful Internet sites and computer software forenzymatic data analysis, Enzymes, Second Edition isthe ultimate practical guide for scientists and students inbiochemical, pharmaceutical, biotechnical, medicinal, andagricultural/food-related research.

Author : Eisenthal Robert (EDS),Danson Michael J.
Publisher : Unknown
Page : 282 pages
File Size : 44,7 Mb
Release : 2006
Category : Electronic
ISBN : OCLC:772581092

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Enzyme Assays- A Practical Approach by Eisenthal Robert (EDS),Danson Michael J. Pdf

Author : S. Gul,S. K. Sreedharan,K. Brocklehurst
Publisher : Wiley-Interscience
Page : 132 pages
File Size : 48,7 Mb
Release : 1998-06-18
Category : Medical
ISBN : UOM:39015046870989

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Enzyme Assays by S. Gul,S. K. Sreedharan,K. Brocklehurst Pdf

Enzyme Assays S. Gul, S.K. Sreedharan and K. Brocklehurst The development of an assay procedure by which the course of a particular enzyme-catalyzed reaction may be monitored is an essential preliminary step in virtually all experimental studies on the enzyme. This book summarizes the key principles underlying enzyme assays, including assay design and the evaluation of experimental data. It provides details of and references to the major physico-chemical techniques commonly used in enzymology. Useful ancillary data are also included on subjects such as active site titration, reagent kits and buffers. The Essential Data series provides rapid access to the core data required by researchers on a daily basis, in convenient pocket-sized volumes.

Author : Seema Anil Belorkar,Sudisha Jogaiah
Publisher : Academic Press
Page : 268 pages
File Size : 45,8 Mb
Release : 2021-11-19
Category : Science
ISBN : 9780323914376

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Protocols and Applications in Enzymology by Seema Anil Belorkar,Sudisha Jogaiah Pdf

Protocols and Applications in Enzymology provides instruction on the experimental procedures of enzyme isolation techniques, innovative screening techniques, and instrument enabled enzyme assays and their underlying principles, among other protocols. The book serves as a one-stop solution for those working with different enzyme protocols in the fields of biochemistry, microbiology, biotechnology and allied subjects. Each chapter offers a full overview of protocol key resources, materials required, quantifiable and statistical analysis, optimization and troubleshooting, safety considerations, and standards. Applications are discussed across distribution and diversity of microbial enzymes, enzyme screening, enzymes in solid state fermentations, enzyme assays, enzyme kinetics, and biotechnological uses. Provides step-by-step instruction on enzyme protocols and applications, with actionable discussions of needed resources, materials, quantification and statistical analysis, optimization and troubleshooting, safety considerations and standards Presents easy to read, reproducible protocols for researchers and students across academia and industry Includes color diagrams that illustrate key concepts

Author : Clyde E. Stauffer
Publisher : Springer
Page : 318 pages
File Size : 46,9 Mb
Release : 1995-12-31
Category : Science
ISBN : 0442207654

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Enzyme Assays for Food Scientists by Clyde E. Stauffer Pdf