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Author : Gareth Griffiths,Brian Burke,John Lucocq
Publisher : Springer
Page : 459 pages
File Size : 55,9 Mb
Release : 1993
Category : Electron microscopic immunocytochemistry
ISBN : 354054805X
Author : Gareth Griffiths
Publisher : Springer Science & Business Media
Page : 477 pages
File Size : 52,5 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642770951
Electron microscopy in the biological sciences can be divided into two disciplines. The first, concerned with high resolution detail of particles or periodic structures, is mostly based on sound theoretical principles of physics. The second, by far the larger discipline, is interested in the information obtainable from thin sections. The theoretical back ground to those groups of techniques for preparing and looking at thin sections is often inexact and "loose", for want of a better word. What should be chemistry is often closer to alchemy. This kind of electron microscopy is often enshrined with mystical recipes, handed down from generation to generation. Admittedly, many of the processes involved, such as those required to embed tissue in epoxy resins, involve multiple interconnected steps, which make it difficult to follow the details of anyone of these steps. If all these steps are shrouded in some mystery, however, can one really trust the final image that emerges on the EM screen? When we present the data in some semi quantitative form is there really no better way to do it than to categorize the parameters with ++, +/-, etc? What happens when one labels the sections with antibodies? Does the whole business necess arily need to be more of an "art" than a "science"? Upon reflecting on these problems in 1981, I had the impression that many of the multi-authored textbooks that existed then (and that have appeared since) tended to exacerbate or at least perpetuate this
Author : Gareth Griffiths,B Burke,J Lucocq
Publisher : Unknown
Page : 486 pages
File Size : 43,8 Mb
Release : 1993-01-26
Category : Electronic
ISBN : 3642770967
Author : Julian E. Beesley
Publisher : Springer Science & Business Media
Page : 277 pages
File Size : 41,7 Mb
Release : 2012-12-06
Category : Science
ISBN : 9781461201397
Immunocytochemistry and in situ hybridization are widely used biomedical sciences. They are essential in medical diagnosis and in cell biology research. Affinity labeling is the central goal of the experimental strategy involving a series of techniques in a logical order; from the effects of specimen fixation, through specimen preparation to expose the antigen, to optimizing immunolabeling, to assessing the result and finally to safety considerations. Numerous examples of these techniques in biomedical sciences are included, as well as experimental assays and practical tips. This survey of methods will serve as an invaluable reference source in any laboratory setting (academic, industrial or clinical) involved in research in almost every branch of biology or medicine, as well as in pharmaceutical, biotechnological and clinical applications.
Author : Simon Renshaw
Publisher : John Wiley & Sons
Page : 258 pages
File Size : 45,5 Mb
Release : 2017-02-06
Category : Science
ISBN : 9781118717776
Immunohistochemistry and immunocytochemistry are invaluable tools for the visualization of tissue and cellular antigens in diagnostic and biological research environments. The need to obtain accurate, reliable and reproducible results is paramount. It is with this fundamental aim in mind that we have compiled Immunohistochemistry: Essential Methods. We have achieved this by examining each aspect of immunochemistry in turn, with each chapter including detailed information regarding the subject matter in question. Each chapter is written by an expert in their field and includes protocols that are typically used in their own research. Subjects covered are, amongst others, antibodies and their production; selection of reporter labels; immunochemical staining methods and experimental design (both using single and multiple reporter labels); quality assurance; automated immunochemistry; confocal microscopy and electron microscopy. In addition, benefits and limitations of each approach are discussed within the chapters.
Author : Anonim
Publisher : ScholarlyEditions
Page : 388 pages
File Size : 51,8 Mb
Release : 2012-12-26
Category : Science
ISBN : 9781464994135
Cytoplasmic Structures—Advances in Research and Application: 2012 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Cytoplasmic Structures. The editors have built Cytoplasmic Structures—Advances in Research and Application: 2012 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Cytoplasmic Structures in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Cytoplasmic Structures—Advances in Research and Application: 2012 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Author : Anonim
Publisher : Academic Press
Page : 516 pages
File Size : 54,7 Mb
Release : 2020-08-20
Category : Science
ISBN : 9780128211588
This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Methods in Enzymology series
Author : Geoffrey R. Newman,Jan A. Hobot
Publisher : Springer Science & Business Media
Page : 225 pages
File Size : 49,7 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642974816
For the first time, fixation, resin embedding and immunocytochemistry, the subjects of innumerable publications, have been organised into a comprehensive and coherent scheme showing how the various methodologies interrelate. Commercially available resins and their mode of use are described, and the book considers the advantages for cytochemistry and immunocytochemistry of matching tissue fixation to processing and resin embedding. On-section single and double immunolabelling methods are covered, using immunocolloidal gold and immunoperoxidase.
Author : Miguel Berrios
Publisher : Academic Press
Page : 659 pages
File Size : 55,6 Mb
Release : 1998
Category : Medical
ISBN : 9780125641555
This volume is a comprehensive guide to the methodologies used in the study of structural domains of cell nuclei. The text covers chromatin, the karyoskeleton, the soluble domain, and the nucleolus. It details methods that are used to isolate components from these domains and techniques used to assemble and disassemble nuclear elements. There is also coverage of three-dimensional mapping and localization of nuclear processes. Key Features * Provides a practical laboratory guide for studying cell nuclei * Includes comprehensive and easy-to-follow protocols
Author : Hesam Dehghani
Publisher : BoD – Books on Demand
Page : 334 pages
File Size : 55,8 Mb
Release : 2012-03-09
Category : Medical
ISBN : 9789535102298
Immunocytochemistry is classically defined as a procedure to detect antigens in cellular contexts using antibodies. However, over the years many aspects of this procedure have evolved within a plethora of experimental setups. There are different ways to prepare a given specimen, different kinds of antibodies to apply, different techniques for imaging, and different methods of analyzing the data. In this book, various ways of performing each individual step of immunocytochemistry in different cellular contexts are exemplified and discussed. Applications of Immunocytochemistry offers technical and background information on different steps of immunocytochemistry and presents the application of this technique and its adaptations in cell lines, neural tissue, pancreatic tissue, sputum cells, sperm cells, preimplantation embryo, arabidopsis, fish gonads, and Leishmania.
Author : Anonim
Publisher : Academic Press
Page : 361 pages
File Size : 51,5 Mb
Release : 2009-11-27
Category : Science
ISBN : 9780080961064
Along with its companion volume on intraflagellar transport, this book provides researchers with a comprehensive and up-to-date source of methods for the analysis cilia and flagella, focusing primarily on approaches that have been devised or significantly extended since the last volume of Methods in Cell Biology on this topic (volume 47, 1995). Edited by Stephen M. King and Gregory J. Pazour, the newest installment of this highly acclaimed serial will serve as an essential addition to the study of cilia and flagella. Covers protocols for cilia and flagella across systems and species Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time Relevant to clinicians interested in respiratory disease, male infertility, and other syndromes who need to learn biochemical, molecular, and genetic approaches to studying cilia, flagella, and related structures
Author : Ennio Pannese
Publisher : Springer
Page : 329 pages
File Size : 42,5 Mb
Release : 2015-03-16
Category : Medical
ISBN : 9783319068565
During the second half of the 20th century, the introduction of a variety of new techniques greatly expanded our knowledge of the structure of nerve and neuroglial cells and of the organization of the nervous system at the cellular level. This new information has, however, generally been scattered throughout the literature, making it difficult for investigators to gain an overall vision of the discipline. The author has marshalled these scattered data to provide a systematic overview of the field placed within a historical context. He has correlated the major new findings with the classical notions of light microscopy and has integrated them with elements of biochemistry and cell physiology. After the publication of the first edition, the introduction of new microscopies and especially the growth of molecular biology have greatly expanded our knowledge, in particular on the intercellular communication in the nervous system and on the roles of neuroglial cells. These achievements have made a second edition of the book necessary. The fully revised and updated text, together with unique high-quality illustrations of structures of the central and peripheral nervous system, ensure that this new edition will be invaluable for neuroscientists, particularly those involved in cellular neuropathology, neurochemistry, neurophysiology and molecular neurobiology.
Author : Edward Chee Tak Yeung,Claudio Stasolla,Michael John Sumner,Bing Quan Huang
Publisher : Springer
Page : 576 pages
File Size : 40,5 Mb
Release : 2015-09-19
Category : Science
ISBN : 9783319199443
A proper understanding of the structural organization of the plant body is essential to any study in plant biology. Experimental studies in vivo and in situ will lead to structural, physiological, and cellular changes of the experimental material. To study macroscopic and microscopic changes, different histological methods and microtechniques can be used as they provide valuable information of the experimental system. In addition, the observed structural changes allow investigators to set hypothesis for further studies based on one’s own observation. Thus, proper selection and utilization of microtechniques are a must for the success of a research program. At present, an up-to-date collection of protocols are not readily available in the literature. The latest work in plant microtechniques was published in 1999 by Ruzin but many others are no longer in print [e.g., Jensen (1964); O’Brien and McCully (1981)]. Furthermore, a majority of published works focus on techniques related to general processing and staining procedures. A comprehensive treatment that encompasses broader applications of microtechniques to other disciplines is lacking [e.g., archeology, wood science, etc.]. There is a need to create a comprehensive volume of botanical methods and protocols which includes traditional and novel techniques that can be used by researchers in plant science and investigators in other disciplines that require plant microtechniques in their research and teaching. This book covers a wide variety of applications and brings them up-to-date to make them understandable and relevant, especially to students using the methods for the first time. It is our intention to create a useful reference for plant histology and related methods that will serve as a foundation for plant scholars, researchers, and teachers in the plant sciences.
Author : Kevin Vaughn
Publisher : Springer Science & Business Media
Page : 131 pages
File Size : 49,5 Mb
Release : 2013-03-27
Category : Science
ISBN : 9789400760615
Immunocytochemistry of plant cells is the first book exclusively dedicated to this topic. The first and largest portion of the book is concerned with a group of proven protocols and variations on these protocols that might prove useful, many developed or modified in the author's laboratory. The second portion of the book covers the studies that have been published previously on each of the plant organelles. Numerous state of the art micrographs from researchers around the world are included to demonstrate typical results.
Author : Thomas Muller-Reichert,Paul Verkade
Publisher : Academic Press
Page : 466 pages
File Size : 46,6 Mb
Release : 2012-08-10
Category : Science
ISBN : 9780124160262
The combination of electron microscopy with transmitted light microscopy (termed correlative light and electron microscopy; CLEM) has been employed for decades to generate molecular identification that can be visualized by a dark, electron-dense precipitate. This new volume of Methods in Cell Biology covers many areas of CLEM, including a brief history and overview on CLEM methods, imaging of intermediate stages of meiotic spindle assembly in C. elegans embryos using CLEM, and capturing endocytic segregation events with HPF-CLEM. Covers many areas of CLEM by the best international scientists in the field Includes a brief history and overview on CLEM methods
