Fluorescence And Phosphorescence Of Proteins And Nucleic Acids

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Fluorescence and Phosphorescence of Proteins and Nucleic Acids

Author : Sergei V. Konev
Publisher : Springer Science & Business Media
Page : 208 pages
File Size : 47,7 Mb
Release : 2012-12-06
Category : Science
ISBN : 9781468407006

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Fluorescence and Phosphorescence of Proteins and Nucleic Acids by Sergei V. Konev Pdf

Fluorescence and phosphorescence are proving to be extremely sensitive probes for elucidating conformation of proteins and nucleic acids and for studying molecular interactions. Newer instrumentation and techniques hold forth great promise for the future of these luminescence methods in biopolymer research. It must be noted, however, that the discovery that certain amino acids, purines, and pyrimidines emit fluorescence or phosphorescence is relatively recent, occurring within the last decade. Professor Konev is one of the pioneers in the application of these procedures to biopolymers and is highly qualified to write about this subject. This book, though written largely as a monograph of the author's own contributions, is also an excellent review of the subject. Of particular interest are the references to many important Russian papers in this field which have not been recognized in the Western literature. It is apparent from this book that fluorescence and phosphorescence methods are being used about as widely in Russia as elsewhere in the world and that we must not overlook these im portant contributions. Konev's studies on protein fluorescence have been widely recognized. It is of interest to learn about these and other of his applications. The last part of the book, which deals with fluorescence as a means to probe into the structure and conforma tion of macromolecules in intact cells, is most interesting. Aside from published symposia this book is the first written specifically about luminescence of biopolymers. Sidney Udenfriend Bethesda. Maryland May, 1967 v CONTENTS Introduction . • • . . . . . . . . . • . . . . . . . . . .

Excited States of Proteins and Nucleic Acids

Author : Robert Steiner
Publisher : Springer Science & Business Media
Page : 497 pages
File Size : 49,6 Mb
Release : 2012-12-06
Category : Science
ISBN : 9781468418781

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Excited States of Proteins and Nucleic Acids by Robert Steiner Pdf

The choice of title for this collective volume reflects the desire of the editors and authors to make clear that, while the bulk of the material is concerned with luminescence, other aspects of the excited state have not been excluded. In the five years which have elapsed since the publication of the classical monograph of Konev, a wealth of new information has ap peared on the emission properties of proteins and nucleic acids. Indeed, since new publications in this area appear to be proliferating in a geometric ratio, this may be the last opportunity to provide a comprehensive summary of the field in a book which is not of prohibitive length. This is what we have attempted to do here. While the orientation of each chapter naturally reflects the interests and point of view of the author, there has been a general effort to present .a critical assessment of existing results and interpretations, rather than a compendium of data with minimal comment. Finally, it should be stressed that the rapid evolution of the subject at the time of writing makes it inevitable that the book will age to some degree over the next few years, although this will occur at differing rates for the various chapters. We can only hope that most of the material in this interim summing-up will prove resistant to the erosion of time and provide a solid foundation for further progress.

Single-Molecule Studies of Nucleic Acids and Their Proteins

Author : David Bensimon,Vincent Croquette,Jean-François Allemand,Xavier Michalet,Terence Strick
Publisher : Oxford University Press
Page : 224 pages
File Size : 48,7 Mb
Release : 2018-11-30
Category : Science
ISBN : 9780192559968

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Single-Molecule Studies of Nucleic Acids and Their Proteins by David Bensimon,Vincent Croquette,Jean-François Allemand,Xavier Michalet,Terence Strick Pdf

This book provides the basis for understanding the elastic properties of nucleic acids (DNA, RNA), the methods used to manipulate them (e.g. optical, magnetic and acoustic tweezers and traps), and how to observe their interactions with proteins (e.g. fluorescence microscopy, FCS, FRET, etc.). It then exemplifies the use of these various methods in the study of three families of DNA enzymes: polymerases, helicases and topoisomerases. The book aims not to be exhaustive, but rather to stimulate the imagination of readers in the application of these single molecule approaches to the study of DNA/RNA and their interactions.

Fluorescence Assay in Biology and Medicine

Author : Sidney Udenfriend
Publisher : Academic Press
Page : 673 pages
File Size : 53,8 Mb
Release : 2014-05-27
Category : Science
ISBN : 9781483258812

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Fluorescence Assay in Biology and Medicine by Sidney Udenfriend Pdf

Molecular Biology: An International Series of Monographs and Textbooks: Fluorescence Assay in Biology and Medicine, Volume II covers the many applications of fluorescence and phosphorescence. This book discusses the principles of fluorescence polarization, comparison of luminescence methods of analysis, and direct measurement of fluorescence decay times. The photodecomposition, sulfhydryl compounds, determination of primary structure, and fluorescent staining are also deliberated. This text likewise covers the assay of purines in nucleic acid hydrolyzates, formyltetrahydrofolate synthetase, and ovarian hormones. This volume is valuable to chemists, physicists, and biophysicists intending to use fluorescence in studying reaction mechanisms and elucidate the structure of complex biopolymers.

Fluorescence Spectroscopy in Biology

Author : Martin Hof,Rudolf Hutterer,V. Fidler
Publisher : Springer Science & Business Media
Page : 315 pages
File Size : 51,8 Mb
Release : 2006-01-27
Category : Science
ISBN : 9783540270041

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Fluorescence Spectroscopy in Biology by Martin Hof,Rudolf Hutterer,V. Fidler Pdf

Volume 3 of this new series focuses on brandnew research and applications in biology, biophysics and other fields of life sciences. Many frontline researcher have contributed to this highly attractive and interdisciplinary volume which spans the entire field of present fluorescence spectroscopy including nanotechnology, membrane and DNA studies and fluorescence imaging in cancer research.

Principles of Fluorescence Spectroscopy

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
Page : 954 pages
File Size : 45,6 Mb
Release : 2007-12-05
Category : Science
ISBN : 9780387463124

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Principles of Fluorescence Spectroscopy by Joseph R. Lakowicz Pdf

The third edition of this established classic text reference builds upon the strengths of its very popular predecessors. Organized as a broadly useful textbook Principles of Fluorescence Spectroscopy, 3rd edition maintains its emphasis on basics, while updating the examples to include recent results from the scientific literature. The third edition includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. Includes a link to Springer Extras to download files reproducing all book artwork, for easy use in lecture slides. This is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.

Photochemistry of Proteins and Nucleic Acids

Author : A. D. McLaren,D. Shugar
Publisher : Elsevier
Page : 462 pages
File Size : 45,6 Mb
Release : 2016-06-06
Category : Science
ISBN : 9781483136035

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Photochemistry of Proteins and Nucleic Acids by A. D. McLaren,D. Shugar Pdf

Photochemistry of Proteins and Nucleic Acids focuses on the effects of ultraviolet and visible radiations on proteins and nucleic acids. The book first discusses some principles of photochemistry, including the laws of photochemistry and factors influencing photochemical reactions in solutions. The text describes absorption and luminescence spectra of nucleoproteins and their components, including principal absorbing groups in proteins, nucleic acids, and nucleoproteins. The selection also highlights the action of ultraviolet light on proteins; photochemical and photosensitized inactivation of enzymes; and the photochemistry of purine and pyrimidine derivatives. The text also discusses nucleic acids and oligo- and polynucleotides. Topics include photochemical degradation of nucleic acid; kinetics of biological inactivation of nucleic acids; nucleoproteins; and reversibility of nucleic acid photolysis. The book also encompasses the inactivation of viruses, including inactivation studies with a plant virus, bacteriophages, and photochemically produced vaccines. The text also presents some problems in photobiology and some techniques in photochemistry. The text is a good source of information for readers interested in the study of proteins and nucleic acids. Based on the standards and codes from Fo

Fluorescence and Phosphorescence Spectroscopy

Author : Stephen G Schulman
Publisher : Elsevier
Page : 298 pages
File Size : 44,9 Mb
Release : 2013-10-22
Category : Science
ISBN : 9781483160993

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Fluorescence and Phosphorescence Spectroscopy by Stephen G Schulman Pdf

Fluorescence and Phosphorescence Spectroscopy: Physicochemical Principles and Practice deals with the physicochemical principles and applications of fluorescence and phosphorescence spectroscopy in experimental biology and chemistry. Topics covered include the absorption of light by molecules; instrumentation for the measurement of fluorescence and phosphorescence; solvent and acidity effects on electronic spectra; and polarization of fluorescence and phosphorescence. Comprised of four chapters, this book begins with a discussion on photophysical processes in isolated molecules and molecules in solution, paying particular attention to thermal equilibration of electronically excited molecules, phototautomerism, and coordination by metal ions. The next chapter describes the instrumentation for measuring fluorescence and phosphorescence, which consists essentially of a light source to electronically excite the sample; a monochromator to separate the light of desired energy from the source; a sample compartment; a second monochromator to isolate the sample's fluorescence energy from the excitation energy; a photodetector to translate the fluorescent light into an electrical signal; and a readout system such as a galvanometer or a recorder, coupled with an amplifier to determine the intensity of fluorescent light that is emitted. The final chapter is devoted to various applications of fluorescence and phosphorescence spectroscopy, including the analysis of organic and inorganic compounds. This monograph is written primarily for analytical chemists and biological scientists.

Biochemical Applications

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
Page : 398 pages
File Size : 47,5 Mb
Release : 1992-02-29
Category : Science
ISBN : 9780306439544

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Biochemical Applications by Joseph R. Lakowicz Pdf

Fluorescence spectroscopy and its applications to the physical and life sciences have evolved rapidly during the past decade. The increased interest in fluorescence appears to be due to advances in time resolution, methods of data analysis and improved instrumentation. With these advances, it is now practical to perform time-resolved measurements with enough resolution to compare the results with the structural and dynamic features of mac- molecules, to probe the structures of proteins, membranes, and nucleic acids, and to acquire two-dimensional microscopic images of chemical or protein distributions in cell cultures. Advances in laser and detector technology have also resulted in renewed interest in fluorescence for clinical and analytical chemistry. Because of these numerous developments and the rapid appearance of new methods, it has become difficult to remain current on the science of fluorescence and its many applications. Consequently, I have asked the experts in particular areas of fluorescence to summarize their knowledge and the current state of the art. This has resulted in the initial three volumes of Topics in Fluorescence Spectroscopy, which is intended to be an ongoing series which summarizes, in one location, the vast literature on fluorescence spectroscopy. These first three volumes are designed to serve as an advanced text. These volumes describe the more recent techniques and technologies (Volume 1), the principles governing fluorescence and the experimental observables (Volume 2), and applications in biochemistry and biophysics (Volume 3).

Protein Fluorescence

Author : Joseph R. Lacowicz
Publisher : Springer Science & Business Media
Page : 310 pages
File Size : 46,5 Mb
Release : 2006-04-18
Category : Science
ISBN : 9780306471025

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Protein Fluorescence by Joseph R. Lacowicz Pdf

The intrinsic or natural fluorescence of proteins is perhaps the most complex area of biochemical fluorescence. Fortunately the fluorescent amino acids, phenylalanine, tyrosine and tryptophan are relatively rare in proteins. Tr- tophan is the dominant intrinsic fluorophore and is present at about one mole % in protein. As a result most proteins contain several tryptophan residues and even more tyrosine residues. The emission of each residue is affected by several excited state processes including spectral relaxation, proton loss for tyrosine, rotational motions and the presence of nearby quenching groups on the protein. Additionally, the tyrosine and tryptophan residues can interact with each other by resonance energy transfer (RET) decreasing the tyrosine emission. In this sense a protein is similar to a three-particle or mul- particle problem in quantum mechanics where the interaction between particles precludes an exact description of the system. In comparison, it has been easier to interpret the fluorescence data from labeled proteins because the fluorophore density and locations could be controlled so the probes did not interact with each other. From the origins of biochemical fluorescence in the 1950s with Prof- sor G. Weber until the mid-1980s, intrinsic protein fluorescence was more qualitative than quantitative. An early report in 1976 by A. Grindvald and I. Z. Steinberg described protein intensity decays to be multi-exponential. Attempts to resolve these decays into the contributions of individual tryp- phan residues were mostly unsuccessful due to the difficulties in resolving closely spaced lifetimes.

Methods in Protein Structure and Stability Analysis

Author : Vladimir N. Uversky,Evgeniĭ Anatolʹevich Permi︠a︡kov
Publisher : Nova Publishers
Page : 382 pages
File Size : 52,7 Mb
Release : 2007
Category : Circular dichroism
ISBN : 1600214045

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Methods in Protein Structure and Stability Analysis by Vladimir N. Uversky,Evgeniĭ Anatolʹevich Permi︠a︡kov Pdf

Luminescent Spectroscopy of Proteins

Author : Eugene A. Permyakov
Publisher : CRC Press
Page : 164 pages
File Size : 51,6 Mb
Release : 2018-01-18
Category : Medical
ISBN : 9781351091114

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Luminescent Spectroscopy of Proteins by Eugene A. Permyakov Pdf

Luminescent Spectroscopy of Proteins is devoted to the method of intrinsic protein luminescence, one of the most popular experimental methods in modern biophysics and biochemistry. The book discusses general physical principles of the luminescence method; spectral properties of the main protein chromophores; and protein luminescence and its use for studies on structural, physico-chemical, and functional properties of proteins. Principles of luminescent spectroscopy are illustrated by real-life applications and problems. Luminescent Spectroscopy of Proteins will be an excellent reference for biophysicists, biochemists, analytical chemists, and other scientists interested in this topic.

Fluorescent Energy Transfer Nucleic Acid Probes

Author : Vladimir V. Didenko
Publisher : Springer Science & Business Media
Page : 368 pages
File Size : 44,7 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781597450690

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Fluorescent Energy Transfer Nucleic Acid Probes by Vladimir V. Didenko Pdf

Fluorescent nucleic acid probes, which use energy transfer, include such constructs as molecular beacons, molecular break lights, Scorpion primers, TaqMan probes, and others. These probes signal detection of their targets by changing either the intensity or the color of their fluorescence. Not surpr- ingly, these luminous, multicolored probes carry more flashy names than their counterparts in the other fields of molecular biology. In recent years, fluor- cent probes and assays, which make use of energy transfer, have multiplied at a high rate and have found numerous applications. However, in spite of this explosive growth in the field, there are no manuals summarizing different p- tocols and fluorescent probe designs. In view of this, the main objective of Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols is to provide such a collection. Oligonucleotides with one or several chromophore tags can form fluor- cent probes capable of energy transfer. Energy transport within the probe can occur via the resonance energy transfer mechanism, also called Förster tra- fer, or by non-Förster transfer mechanisms. Although the probes using Förster transfer were developed and used first, the later non-Förster-based probes, such as molecular beacons, now represent an attractive and widely used option. The term “fluorescent energy transfer probes” in the title of this book covers both Förster-based fluorescence resonance energy transfer (FRET) probes and probes using non-FRET mechanisms. Energy transfer probes serve as molecule-size sensors, changing their fluorescence upon detection of various DNA reactions.

Selected Technical Publications

Author : Anonim
Publisher : Unknown
Page : 704 pages
File Size : 54,7 Mb
Release : 1970
Category : Drugs
ISBN : IND:30000090151550

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Selected Technical Publications by Anonim Pdf