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A second edition of the classic handbook has become a standard in the Drosophila field. This edition is expanded to include topics in which classical genetic strategies have been augmented with new molecular tools. Included are such new techniques as homologous recombination, RNAi, new mapping techniques, and new mosaic marking techniques.
Understanding how a multicellular animal develops from a single cell (the fertilized egg) poses one of the greatest challenges in biology today. Development from egg to adult involves the sequential expression of virtually the whole of an organism's genetic instructions both in the mother as she lays down developmental cues in the egg, and in the embryo itself. Most of our present information on the role of genes in development comes from the invertebrate fruit fly, Drosophila. The two authors of this text (amongst the foremost authorities in the world) follow the developmental process from fertilization through the primitive structural development of the body plan of the fly after cleavage into the differentiation of the variety of tissues, organs and body parts that together define the fly. The developmental processes are fully explained throughout the text in the modern language of molecular biology and genetics. This text represents the vital synthesis of the subject that many have been waiting for and it will enable many specific courses in developmental biology and molecular genetics to focus on it. It will appeali to 2nd and 3rd year students in these disciplines as well as in biochemistry, neurobiology and zoology. It will also have widespread appeal among researchers. Authored by one of the foremost authorities in the world. A unique synthesis of the developmental cycle of Drosophila - our major source of information on the role of genes in development. Designed to provide the basis of new courses in developmental biology and molecular genetics at senior undergraduate level. A lucid explanation in the modern language of the science.
Drosophila Neurobiology by Bing Zhang,Marc R. Freeman,Scott Waddell Pdf
Based on Cold Spring Harbor Laboratory's long-running course, Drosophila Neurobiology: A Laboratory Manual offers detailed protocols and background material for researchers interested in using Drosophila as an experimental model for investigating the nervous system. This manual covers three approaches to the field: analysis of neural development, recording and imaging activities in the nervous system, and analysis of behavior. Techniques described include molecular, genetic, electrophysiological, imaging, behavioral and developmental methods.
A plane crashes on a desert island and the only survivors, a group of schoolboys, assemble on the beach and wait to be rescued. By day they inhabit a land of bright fantastic birds and dark blue seas, but at night their dreams are haunted by the image of a terrifying beast. As the boys' delicate sense of order fades, so their childish dreams are transformed into something more primitive, and their behaviour starts to take on a murderous, savage significance. First published in 1954, Lord of the Flies is one of the most celebrated and widely read of modern classics. Now fully revised and updated, this educational edition includes chapter summaries, comprehension questions, discussion points, classroom activities, a biographical profile of Golding, historical context relevant to the novel and an essay on Lord of the Flies by William Golding entitled 'Fable'. Aimed at Key Stage 3 and 4 students, it also includes a section on literary theory for advanced or A-level students. The educational edition encourages original and independent thinking while guiding the student through the text - ideal for use in the classroom and at home.
First contact with extraordinary aliens, glittering technologies that could destroy the universe in a nanosecond, huge sweeping space operas: Alastair Reynolds is back! Some centuries from now, the exploration and exploitation of the Solar System is in full swing. On the cold edge of the system, Bella Lind, captain of the huge commercial spacecraft Rockhopper IV, helps fuel this new gold rush by attaching mass-driver motors to organic-rich water-ice comets to move them back to the inner worlds. Her crew are tough, blue-collar miners, engineers and demolition experts. Around Saturn, something inexplicable happens: one of the moons leaves its orbit and accelerates out of the Solar System. The icy mantle peels away to reveal that it was never a moon in the first place, just a parked spacecraft, millions of years old, that has now decided to move on. Rockhopper IV, trapped in the pull, is hurled across time and space into the deep, distant future, arriving in a vast, alien-constructed chamber. And the crew are not alone, for each chamber contains an alien culture dragged into this cosmic menagerie at the end of time. The crew of the Rockhopper IV know a lot about blowing up comets, but not much about first contact with ultra-advanced aliens. They have two things to worry about: can they (and their new alien allies) negotiate their way through each harrying contact? And can they assimilate the avalanche of knowledge about their own future - including all the glittering, dangerous technologies that are now theirs for the taking - without destroying themselves in the process?
Biotechnology in Healthcare, Volume 2 by Debmalya Barh Pdf
Biotechnology in Healthcare presents up-to-date knowledge on the emerging field of biotechnology as applied to the healthcare industry. Biotechnology has revolutionized healthcare in the last two decades by developing and introducing novel diagnostics, therapeutics, and preventive measures; whether it is noncommunicable or communicable disease, primary or secondary care, or public health, it has shown its immense potential to provide a solution to the healthcare providers, physicians, and allied health care professionals.The second volume, Applications and Initiatives, contains 19 chapters focused on the applications of biotechnology related to public healthcare, hospital management, oncology, neurodegenerative and infectious diseases, regenerative medicine, IVF, clinical trials, precision food, FMGCs, PPCPs, pharmaceuticals, and smart technologies to monitor pandemic. Further, this volume also presents government initiatives and entrepreneurship challenges in healthcare biotechnology sector.This is a valuable resource for students, biotechnologists, bioinformaticians, clinicians, and members of biomedical and healthcare fields who need to understand more about the promising developments of the emerging field of biotechnology in healthcare. Describes various applications of novel biotechnology approaches in healthcare Presents applications of biotechnology in primary and secondary healthcare and in public health Discusses government initiatives, challenges and opportunities, and entrepreneurship development in the area of healthcare biotechnology
#1 NEW YORK TIMES BESTSELLER • From the indie rock sensation known as Japanese Breakfast, an unforgettable memoir about family, food, grief, love, and growing up Korean American—“in losing her mother and cooking to bring her back to life, Zauner became herself” (NPR). • CELEBRATING OVER ONE YEAR ON THE NEW YORK TIMES BESTSELLER LIST In this exquisite story of family, food, grief, and endurance, Michelle Zauner proves herself far more than a dazzling singer, songwriter, and guitarist. With humor and heart, she tells of growing up one of the few Asian American kids at her school in Eugene, Oregon; of struggling with her mother's particular, high expectations of her; of a painful adolescence; of treasured months spent in her grandmother's tiny apartment in Seoul, where she and her mother would bond, late at night, over heaping plates of food. As she grew up, moving to the East Coast for college, finding work in the restaurant industry, and performing gigs with her fledgling band--and meeting the man who would become her husband--her Koreanness began to feel ever more distant, even as she found the life she wanted to live. It was her mother's diagnosis of terminal cancer, when Michelle was twenty-five, that forced a reckoning with her identity and brought her to reclaim the gifts of taste, language, and history her mother had given her. Vivacious and plainspoken, lyrical and honest, Zauner's voice is as radiantly alive on the page as it is onstage. Rich with intimate anecdotes that will resonate widely, and complete with family photos, Crying in H Mart is a book to cherish, share, and reread.
Principles of Development by Lewis Wolpert,Cheryll Tickle,Alfonso Martinez Arias Pdf
Developmental biology is at the core of all biology. This text emphasises the principles and key developments in order to provide an approach and style that will appeal to students at all levels.
Drosophila melanogaster by Farzana Khan Perveen Pdf
This book contains 12 chapters divided into two sections. Section 1 is "Drosophila - Model for Genetics." It covers introduction, chromosomal polymorphism, polytene chromosomes, chromosomal inversion, chromosomal evolution, cell cycle regulators in meiosis and nongenetic transgenerational inheritance in Drosophila. It also includes ecological genetics, wild-type strains, morphometric analysis, cytostatics, frequencies of early and late embryonic lethals (EEL and LEL) and mosaic imaginal discs of Drosophila for genetic analysis in biomedical research. Section 2 is "Drosophila - Model for Therapeutics." It explains Drosophila as model for human diseases, neurodegeneration, heart-kidney metabolic disorders, cancer, pathophysiology of Parkinson's disease, dopamine, neuroprotective therapeutics, mitochondrial dysfunction and translational research. It also covers Drosophila role in ubiquitin-carboxyl-terminal hydrolase-L1 (UCH-L1) protein, eye development, anti-dUCH antibody, neuropathy target esterase (NTE), organophosphorous compound-induced delayed neuropathy (OPIDN) and hereditary spastic paraplegia (HSP). It also includes substrate specificities, kinetic parameters of recombinant glutathione S-transferases E6 and E7 (DmGSTE6 and DmGSTE7), detoxification and insecticidal resistance and antiviral immunity in Drosophila.
Handbook of Models for Human Aging by P. Michael Conn Pdf
The Handbook of Models for Human Aging is designed as the only comprehensive work available that covers the diversity of aging models currently available. For each animal model, it presents key aspects of biology, nutrition, factors affecting life span, methods of age determination, use in research, and disadvantages/advantes of use. Chapters on comparative models take a broad sweep of age-related diseases, from Alzheimer's to joint disease, cataracts, cancer, and obesity. In addition, there is an historical overview and discussion of model availability, key methods, and ethical issues. Utilizes a multidisciplinary approach Shows tricks and approaches not available in primary publications First volume of its kind to combine both methods of study for human aging and animal models Over 200 illustrations
Methods in Kidney Cell Biology Part B by Anonim Pdf
Methods in Kidney Cell Biology, Part B, Volume 154 represents state-of-the-art techniques in renal research that are ideal for veterans, graduate students, postdoctoral fellows, clinical scientists and principal investigators. Topics in the new release include Single glomerular proteomics – a novel method in translational glomerular cell biology, Measurement of cytosolic and intraciliary calcium in live cells, Differentiation of human kidney organoids from pluripotent stem cells, Quantifying autophagic flux in kidney tissue using structured illumination microscopy, the Generation of primary cells from ADPKD and normal human kidneys, ADPKD cell proliferation and Cl-dependent fluid secretion, In vitro cyst formation of ADPKD cells, and much more. Written by experts in their field who have perfected stated methods Covers a wide range of topics, from state-of-the-art techniques that may require specialized equipment, to tried-and-true classic methods in their most refined form Includes cutting-edge, recently developed methods
Never Fly Solo: Lead with Courage, Build Trusting Partnerships, and Reach New Heights in Business by Robert "Waldo" Waldman Pdf
The New York Times and Wall Street Journal bestselling book on building powerful, effective teams! “An amazing book that bucks the old paradigm that maverick leaders and self-madeentrepreneurs set the bar. It will forever change the way you look at leadership.” —Marshall Goldsmith, author of The Wall Street Journal #1 business bestsellerWhat Got You Here Won’t Get You There “In Never Fly Solo, Rob ‘Waldo’ Waldman shares potent, personal leadership lessonson what it takes to access the power of your wingmen. If you want a former fi ghter pilot with 65combat missions as your wingman and if business success is your target, take flight withWaldo’s book and earn your wings. You’ll never fly solo again.” —Jeffrey Gitomer, bestselling author of The Little Red Book of Selling “If you are serious about building trusting relationships and launching your business and lifeto new heights, then invest in yourself and buy this book.” —Keith Ferrazzi, author of the New York Times #1 bestseller Who’s Got Your Back “Authentic and inspirational, Never Fly Solo is a winning formula for successfulleadership that everyone in business should read.” —Howard Putnam, former CEO of Southwest Airlines and author of The Winds of Turbulence “Waldo does a superb job of applying to the business world the lessons he learned in hismilitary career. His excellent use of vignettes shows how standards and values areapplicable to leading an honorable life.” —General Ron Fogleman, U.S. Air Force Retired, former Chief of Staff, USAF “Waldo the Wingman knows the value of relationships and how to build realpartnerships that benefit everyone involved.” —Mark Sanborn, speaker and bestselling author of The Fred Factor and You Don’t Need a Title to Be a Leader Rob “Waldo” Waldmanunderstands one thing better than most businesspeople:You can’t reach your highest potential alone.You need wingmen—trusted partners—who canhelp you overcome obstacles, adapt to change, andprepare for success. A decorated former combatF-16 fighter pilot and now a successful entrepreneur,Waldman lives his life by this rule. Whether you’re a senior executive, mid-level manager,or new hire fresh out of college, your success dependson the mutual support of trusted associates. In NeverFly Solo, Waldo helps you maximize your relationshipsto reach your greatest potential. You’ll learn how to: Commit to the core wingman values ofintegrity, accountability, service, and excellence Transform your relationships with colleaguesinto interdependent partnerships for success Take courageous action and ask for helpwhen adversity strikes Communicate effectively in high-stresssituations Connect with your coworkers and customerson a deeper level Through compelling, real-world stories, Waldo relateshow his Air Force wingmen helped him overcomechallenges and become successful by teaching himlessons that work in an offi ce as well as in a cockpit. Like the world of aerial combat, today’s high-stakesbusiness world operates faster than the speed ofsound. Teamwork and trust are critical for thwartingthe missiles of fear, change, and risk that too oftensend even the most seasoned professional into adownward spiral. You can’t dodge these missiles alone. Never Fly Solois your flight plan for taking positive action that leadsto greater success. Find out more at www.neverflysolo.com. The author will donate a portion of the book's proceeds to veterans charities.
Orvis Guide to Prospecting for Trout, New and Revised by Tom Rosenbauer Pdf
In this comprehensive and readable guide, Tom Rosenbauer shares his vast knowledge of fly fishing when there is no hatch. Written for both the novice and the seasoned angler, The Orvis Guide to Prospecting for Trout, New and Revised explores how trout live and feed, and how to make them strike, with a thoroughly updated text that addresses state-of-the-art approaches, and all new color photography. There is expert advice on how to fish with dry flies, wet flies, nymphs, and streamers—supported by many detailed illustrations and photos. This is the guide no trout fisherman should be without.