Gram Stain

Author : Subhash K. Mohan
Publisher : AuthorHouse
Page : 190 pages
File Size : 51,6 Mb
Release : 2009-07
Category : Reference
ISBN : 9781438960289

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Gram Stain by Subhash K. Mohan Pdf

There are several medical mycology textbooks that contain a chapter on direct microscopy. However, this textbook is the first of its kind, as it discusses the simple Gram stain procedure as a valuable tool for the detection of fungal elements. This book has been specifically designed for people working in the medical microbiology laboratory with little or no practical experience in medical mycology. The central idea presented in this textbook begins with the Gram stain for the detection of fungi; the most important and more frequently isolated opportunistic and potentially pathogenic fungal species have been included. The book contains more than three hundred color images, the majority of which come from direct smear examination, such as Gram stain and other staining procedures. The mold phase and the microscopic structure of the identified fungal species relating to the initial findings of the direct smear have been linked to avoid bias. When a fungal infection is present but not suspected clinically, the Gram stain may be the only clue to the true cause of the infection. Although there are better methods than the Gram stain for visualization of fungi, these methods are only performed if there is clinical suspicion for fungal disease. Clinicians often send specimens for bacterial culture, but they sometimes overlook requests for fungal culture. During such times, the Gram stain is the only technique available in the clinical microbiology laboratory for direct detection of fungi from these specimens. The presence of fungi should not be overlooked during the direct examination of the clinical specimens for bacteria. This book will guide the reader in the recognition and identification of fungal elements in gram-stained smears, especially when they are distorted and remain unstained and undetectable.This new textbook focuses on the detection and classification of fungal elements in Gram stains. Newly developed flowcharts, clues, and key details regarding structural characteristics have been added to guide the reader in the right direction.Throughout the years, the author has accumulated many scenarios in which fungal elements were not detected on the original Gram stain evaluation but were found to be positive upon review once the culture grew a fungus. Finally, the book contains a chapter with a practice examination including microscopic images representative of scenarios commonly encountered in the clinical microbiology laboratory.

Author : Gilda L. Jones
Publisher : Unknown
Page : 92 pages
File Size : 47,8 Mb
Release : 1984
Category : Bacteriology
ISBN : MINN:31951003072863H

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The Gram Stain by Gilda L. Jones Pdf

Author : Nina Parker,OpenStax,Mark Schneegurt,AnhHue Thi Tu,Brian M. Forster,Philip Lister
Publisher : Unknown
Page : 1301 pages
File Size : 52,5 Mb
Release : 2016-05-30
Category : Microbiology
ISBN : 1938168143

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Microbiology by Nina Parker,OpenStax,Mark Schneegurt,AnhHue Thi Tu,Brian M. Forster,Philip Lister Pdf

"Microbiology covers the scope and sequence requirements for a single-semester microbiology course for non-majors. The book presents the core concepts of microbiology with a focus on applications for careers in allied health. The pedagogical features of the text make the material interesting and accessible while maintaining the career-application focus and scientific rigor inherent in the subject matter. Microbiology's art program enhances students' understanding of concepts through clear and effective illustrations, diagrams, and photographs. Microbiology is produced through a collaborative publishing agreement between OpenStax and the American Society for Microbiology Press. The book aligns with the curriculum guidelines of the American Society for Microbiology."--BC Campus website.

Author : Linda M. Marler,Jean A. Siders,Stephen D. Allen (MD.)
Publisher : Lippincott Williams & Wilkins
Page : 328 pages
File Size : 44,9 Mb
Release : 2001
Category : Medical
ISBN : 0781726638

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Direct Smear Atlas by Linda M. Marler,Jean A. Siders,Stephen D. Allen (MD.) Pdf

This atlas is a full-color, spiralbound manual of Gram-stained direct smears for medical technologist and medical laboratory technician students and practitioners, microbiologists, pathologists, infectious disease specialists, and anyone who interprets Gram-stained specimens. Featuring over 250 clear photomicrographs taken from actual patient specimens, the manual presents a simple, easy-to-use format; brief legends accompany each image. Three introductory chapters cover Gram-stain procedures, quality control, evaluation, and more. An invaluable reference and educational tool, this manual is effective both as a standalone text and as an accompaniment to Marler, Siders and Allen's Direct Smear Atlas CD-ROM (Lippincott Williams & Wilkins, 1998).

Author : Holly Ahern
Publisher : Unknown
Page : 140 pages
File Size : 42,7 Mb
Release : 2018-05-22
Category : Microbiology
ISBN : 1942341539

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Microbiology by Holly Ahern Pdf

As a group of organisms that are too small to see and best known for being agents of disease and death, microbes are not always appreciated for the numerous supportive and positive contributions they make to the living world. Designed to support a course in microbiology, Microbiology: A Laboratory Experience permits a glimpse into both the good and the bad in the microscopic world. The laboratory experiences are designed to engage and support student interest in microbiology as a topic, field of study, and career. This text provides a series of laboratory exercises compatible with a one-semester undergraduate microbiology or bacteriology course with a three- or four-hour lab period that meets once or twice a week. The design of the lab manual conforms to the American Society for Microbiology curriculum guidelines and takes a ground-up approach -- beginning with an introduction to biosafety and containment practices and how to work with biological hazards. From there the course moves to basic but essential microscopy skills, aseptic technique and culture methods, and builds to include more advanced lab techniques. The exercises incorporate a semester-long investigative laboratory project designed to promote the sense of discovery and encourage student engagement. The curriculum is rigorous but manageable for a single semester and incorporates best practices in biology education.

Author : U. S. Department Human Services,Agency for and Quality
Publisher : Createspace Independent Publishing Platform
Page : 0 pages
File Size : 47,7 Mb
Release : 2013-08
Category : Electronic
ISBN : 1491256443

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Screening for Methicillin-Resistant Staphylococcus Aureus (Mrsa) by U. S. Department Human Services,Agency for and Quality Pdf

Methicillin-resistant Staphylococcus aureus (MRSA) emerged as a clinically relevant human pathogen more than five decades ago. The virulent bacterium was first detected in hospitals and other health care facilities where vulnerable hosts, frequent exposure to the selective pressure of intensive antimicrobial therapy, and the necessity for invasive procedures created a favorable environment for dissemination. MRSA emerged as an important cause of healthcare-associated infections, particularly central line-associated bloodstream infection, ventilator-associated pneumonia, and surgical site infection (SSI). Despite the adoption of infection-control measures, the incidence of MRSA infection at most U.S. hospitals steadily increased for many years, but it is now decreasing. While the decrease in the incidence of MRSA infection may be due to efforts to screen for MRSA carriage, it may also be due to secular trends (such as efforts to improve patient safety) and to confounders (such as efforts to improve the appropriate use of antibiotics and to decrease healthcare-associated infections in general, including catheter-associated bloodstream infection, ventilator-associated pneumonia, and SSI). A number of analyses suggest that MRSA infections are associated with increased mortality and cost of care when compared with those due to strains that are susceptible to methicillin. Even the availability of newer pharmaceutical agents with specific activity against MRSA has not ameliorated the challenge of caring for patients with MRSA. The widespread use of these agents has been limited, in part due to toxicity, cost, and uncertainty as to optimal indications. The management and control of MRSA have been further complicated by dramatic changes in the epidemiology of transmission and infection observed over the past two decades. Specifically, S. aureus strains resistant to methicillin, once exclusively linked to hospital care, have increasingly been detected among patients in the community who lack conventional risk factors for MRSA infection. Community-acquired MRSA has been linked to outbreaks of infection in hospitals and health care facilities. Conventional strategies for the control of MRSA have focused on the prevention of spread from patient to patient. The effectiveness of hand hygiene in preventing the spread of MRSA has been demonstrated in observational studies in which hand hygiene promotion campaigns were associated with subsequent reductions in the incidence of MRSA among hospitalized patients. While hand hygiene remains important in the effort to control MRSA transmission, the continued spread of the pathogen after its initial introduction in most facilities has prompted efforts to identify additional strategies. The use of contact isolation-including the donning of gowns and gloves when interacting with patients colonized or infected with MRSA and the assignment of such patients to single rooms or to a room with a group of affected patients-has been widely promoted and adopted. Such isolation precautions now are the centerpiece of most authoritative guidelines for MRSA control. Despite the broad consensus associated with the use of contact isolation for MRSA prevention, the specific evidence in support of this practice remains limited and indirect. The objective of this review was to synthesize comparative studies that examined the benefits or harms of screening for MRSA carriage in the inpatient or outpatient settings. The review examined MRSA-screening strategies applied to all hospitalized or ambulatory patients, as well as screening strategies applied to selected inpatient or outpatient populations, and compared them with no screening or with screening of selected patient populations. The review evaluated MRSA-screening strategies that included screening with or without isolation and with or without attempted eradication/decolonization.

Author : Vincent A. Fischetti,Richard P. Novick,Joseph J. Ferretti,Julian I. Rood,Daniel A. Portnoy
Publisher : Amer Society for Microbiology
Page : 849 pages
File Size : 45,8 Mb
Release : 2006
Category : Medical
ISBN : 1555813437

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Gram-positive Pathogens by Vincent A. Fischetti,Richard P. Novick,Joseph J. Ferretti,Julian I. Rood,Daniel A. Portnoy Pdf

Provides comprehensive coverage of all gram-positive pathogens, including genome data. Appeals to clinicians, infectious disease specialists, and instructors and students seeking a single reference source on gram-positive bacteria.

Author : Anonim
Publisher : John Wiley & Sons
Page : 2954 pages
File Size : 40,5 Mb
Release : 2020-08-06
Category : Medical
ISBN : 9781555818814

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Clinical Microbiology Procedures Handbook by Anonim Pdf

In response to the ever-changing needs and responsibilities of the clinical microbiology field, Clinical Microbiology Procedures Handbook, Fourth Edition has been extensively reviewed and updated to present the most prominent procedures in use today. The Clinical Microbiology Procedures Handbook provides step-by-step protocols and descriptions that allow clinical microbiologists and laboratory staff personnel to confidently and accurately perform all analyses, including appropriate quality control recommendations, from the receipt of the specimen through processing, testing, interpretation, presentation of the final report, and subsequent consultation.

Author : Gilda L. Jones
Publisher : Unknown
Page : 84 pages
File Size : 52,6 Mb
Release : 1986
Category : Bacteriology
ISBN : UOM:39015009124465

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The Gram Stain by Gilda L. Jones Pdf

Author : Luis M. de la Maza,Marie T. Pezzlo,Cassiana E. Bittencourt,Ellena M. Peterson
Publisher : John Wiley & Sons
Page : 464 pages
File Size : 50,8 Mb
Release : 2020-07-15
Category : Science
ISBN : 9781683670353

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Color Atlas of Medical Bacteriology by Luis M. de la Maza,Marie T. Pezzlo,Cassiana E. Bittencourt,Ellena M. Peterson Pdf

This unique visual reference presents more than 750 brilliant, four-color images of bacterial isolates commonly encountered in diagnostic microbiology and the methods used to identify them, including microscopic and phenotypic characteristics, colony morphology, and biochemical properties. Chapters cover the most important bacterial pathogens and related organisms, including updated taxonomy, epidemiology, pathogenicity, laboratory and antibiotic susceptibility testing, and molecular biology methodology Tables summarize and compare key biochemical reactions and other significant characteristics New to this edition is a separate chapter covering the latest developments in total laboratory automation The comprehensive chapter on stains, media, and reagents is now augmented with histopathology images A new Fast Facts chapter presents tables that summarize and illustrate the most significant details for some of the more commonly encountered organisms For the first time, this easy-to-use atlas is available digitally for enhanced searching. Color Atlas of Medical Bacteriology remains the most valuable illustrative supplement for lectures and laboratory presentations, as well as for laboratorians, clinicians, students, and anyone interested in diagnostic medical bacteriology.

Author : J.-M. Ghuysen,R. Hakenbeck
Publisher : Elsevier
Page : 580 pages
File Size : 53,9 Mb
Release : 1994-02-09
Category : Science
ISBN : 0080860877

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Bacterial Cell Wall by J.-M. Ghuysen,R. Hakenbeck Pdf

Studies of the bacterial cell wall emerged as a new field of research in the early 1950s, and has flourished in a multitude of directions. This excellent book provides an integrated collection of contributions forming a fundamental reference for researchers and of general use to teachers, advanced students in the life sciences, and all scientists in bacterial cell wall research. Chapters include topics such as: Peptidoglycan, an essential constituent of bacterial endospores; Teichoic and teichuronic acids, lipoteichoic acids, lipoglycans, neural complex polysaccharides and several specialized proteins are frequently unique wall-associated components of Gram-positive bacteria; Bacterial cells evolving signal transduction pathways; Underlying mechanisms of bacterial resistance to antibiotics.

Author : Jeffrey C. Pommerville
Publisher : Jones & Bartlett Learning
Page : 372 pages
File Size : 49,7 Mb
Release : 2007
Category : Medical microbiology
ISBN : 0763743038

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Alcamo's Laboratory Fundamentals of Microbiology by Jeffrey C. Pommerville Pdf

Microbiology

Author : Azra Hasan
Publisher : Elsevier Health Sciences
Page : 11 pages
File Size : 45,5 Mb
Release : 2019-10-31
Category : Medical
ISBN : 9788131260265

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Gram's Staining by Azra Hasan Pdf

Gram's Staining

Author : Robert A. Britton,Patrice D. Cani
Publisher : John Wiley & Sons
Page : 514 pages
File Size : 44,9 Mb
Release : 2020-07-02
Category : Medical
ISBN : 9781555819705

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Bugs as Drugs by Robert A. Britton,Patrice D. Cani Pdf

Examining the enormous potential of microbiome manipulation to improve health Associations between the composition of the intestinal microbiome and many human diseases, including inflammatory bowel disease, cardiovascular disease, metabolic disorders, and cancer, have been elegantly described in the past decade. Now, whole-genome sequencing, bioinformatics, and precision gene-editing techniques are being combined with centuries-old therapies, such as fecal microbiota transplantation, to translate current research into new diagnostics and therapeutics to treat complex diseases. Bugs as Drugs provides a much-needed overview of microbes in therapies and will serve as an excellent resource for scientists and clinicians as they carry out research and clinical studies on investigating the roles the microbiota plays in health and disease. In Bugs as Drugs, editors Robert A. Britton and Patrice D. Cani have assembled a fascinating collection of reviews that chart the history, current efforts, and future prospects of using microorganisms to fight disease and improve health. Sections cover traditional uses of probiotics, next-generation microbial therapeutics, controlling infectious diseases, and indirect strategies for manipulating the host microbiome. Topics presented include: How well-established probiotics support and improve host health by improving the composition of the intestinal microbiota of the host and by modulating the host immune response. The use of gene editing and recombinant DNA techniques to create tailored probiotics and to characterize next-generation beneficial microbes. For example, engineering that improves the anti-inflammatory profile of probiotics can reduce the number of colonic polyps formed, and lactobacilli can be transformed into targeted delivery systems carrying therapeutic proteins or bioengineered bacteriophage. The association of specific microbiota composition with colorectal cancer, liver diseases, osteoporosis, and inflammatory bowel disease. The gut microbiota has been proposed to serve as an organ involved in regulation of inflammation, immune function, and energy homeostasis. Fecal microbiota transplantation as a promising treatment for numerous diseases beyond C. difficile infection. Practical considerations for using fecal microbiota transplantation are provided, while it is acknowledged that more high-quality evidence is needed to ascertain the importance of strain specificity in positive treatment outcomes. Because systems biology approaches and synthetic engineering of microbes are now high-throughput and cost-effective, a much wider range of therapeutic possibilities can be explored and vetted.

Author : Jeffrey J. Zimmerman,Locke A. Karriker,Alejandro Ramirez,Kent J. Schwartz,Gregory W. Stevenson,Jianqiang Zhang
Publisher : John Wiley & Sons
Page : 1136 pages
File Size : 46,7 Mb
Release : 2019-03-25
Category : Medical
ISBN : 9781119350903

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Diseases of Swine by Jeffrey J. Zimmerman,Locke A. Karriker,Alejandro Ramirez,Kent J. Schwartz,Gregory W. Stevenson,Jianqiang Zhang Pdf

Provides a fully revised Eleventh Edition of the definitive reference to swine health and disease Diseases of Swine has been the definitive reference on swine health and disease for over 60 years. This new edition has been completely revised to include the latest information, developments, and research in the field. Now with full color images throughout, this comprehensive and authoritative resource has been redesigned for improved consistency and readability, with a reorganized format for more intuitive access to information. Diseases of Swine covers a wide range of essential topics on swine production, health, and management, with contributions from more than 100 of the foremost international experts in the field. This revised edition makes the information easy to find and includes expanded information on welfare and behavior. A key reference for anyone involved in the swine industry, Diseases of Swine, Eleventh Edition: Presents a thorough revision to the gold-standard reference on pig health and disease Features full color images throughout the book Includes information on the most current advances in the field Provides comprehensive information on swine welfare and behavior Offers a reorganized format to make the information more accessible Written for veterinarians, academicians, students, and individuals and agencies responsible for swine health and public health, Diseases of Swine, Eleventh Edition is an essential guide to swine health.