Handbook Of Biomedical Nonlinear Optical Microscopy

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Handbook of Biomedical Nonlinear Optical Microscopy

Author : Barry R. Masters,Peter So
Publisher : Oxford University Press
Page : 896 pages
File Size : 48,7 Mb
Release : 2008-05-19
Category : Science
ISBN : 0198036825

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Handbook of Biomedical Nonlinear Optical Microscopy by Barry R. Masters,Peter So Pdf

The Handbook of Biomedical Nonlinear Optical Microscopy provides comprehensive treatment of the theories, techniques, and biomedical applications of nonlinear optics and microscopy for cell biologists, life scientists, biomedical engineers, and clinicians. The chapters are separated into basic and advanced sections, and provide both textual and graphical illustrations of all key concepts. The more basic sections are aimed at life scientists without advanced training in physics and mathematics, and tutorials are provided for the more challenging sections. The first part of the Handbook introduces the historical context of nonlinear microscopy. The second part presents the nonlinear optical theory of two- and multiphoton excited fluorescence (TPE, MPE) spectroscopy, second and third harmonic generation (SHG, THG) spectroscopy, and coherent anti-Stokes Raman spectroscopy (CARS). The third part introduces modern microscopic and spectroscopic instrumentation and techniques that are based on nonlinear optics. The fourth part provides key applications of nonlinear microscopy to the biomedical area: neurobiology, immunology, tumor biology, developmental biology, dermatology, and cellular metabolism. There are also chapters on nonlinear molecular probes, cellular damage, and nanoprocessing.

Foundations of Nonlinear Optical Microscopy

Author : Eric Olaf Potma
Publisher : John Wiley & Sons
Page : 453 pages
File Size : 53,9 Mb
Release : 2024-03-13
Category : Science
ISBN : 9781119814900

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Foundations of Nonlinear Optical Microscopy by Eric Olaf Potma Pdf

Foundations of Nonlinear Optical Microscopy Concise yet comprehensive resource presenting the foundations of nonlinear optical microscopy Foundations of Nonlinear Optical Microscopy brings together all relevant principles of nonlinear optical (NLO) microscopy, presenting NLO microscopy within a consistent framework to allow for the origin of the signals and the interrelation between different NLO techniques to be understood. The text provides rigorous yet practical derivations, which amount to expressions that can be directly related to measured values of resolution, sensitivity, and imaging contrast. The book also addresses typical questions students ask, and answers them with clear explanations and examples. Readers of this book will develop a solid physical understanding of NLO microscopy, appreciate the advantages and limitations of each technique, and recognize the exciting possibilities that lie ahead. Foundations of Nonlinear Optical Microscopy covers sample topics such as: Light propagation, focusing of light, pulses of light, classical description of light-matter interactions, and quantum mechanical description of light-matter interactions Molecular transitions, selection rules, signal radiation, and detection of light Multi-photon fluorescence and pump-probe microscopy Harmonic generation, sum-frequency generation, and coherent Raman scattering Senior undergraduate and graduate students in chemistry, physics, and biomedical engineering, along with students of electrical engineering and instructors in both of these fields, can use the information within Foundations of Nonlinear Optical Microscopy and the included learning resources to gain a concise yet comprehensive overview of the subject.

Handbook of Biomedical Optics

Author : David A. Boas,Constantinos Pitris,Nimmi Ramanujam
Publisher : CRC Press
Page : 816 pages
File Size : 54,6 Mb
Release : 2016-04-19
Category : Medical
ISBN : 9781420090376

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Handbook of Biomedical Optics by David A. Boas,Constantinos Pitris,Nimmi Ramanujam Pdf

Biomedical optics holds tremendous promise to deliver effective, safe, non- or minimally invasive diagnostics and targeted, customizable therapeutics. Handbook of Biomedical Optics provides an in-depth treatment of the field, including coverage of applications for biomedical research, diagnosis, and therapy. It introduces the theory and fundamental

Superresolution Optical Microscopy

Author : Barry R. Masters
Publisher : Springer Nature
Page : 415 pages
File Size : 55,8 Mb
Release : 2020-03-21
Category : Science
ISBN : 9783030216917

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Superresolution Optical Microscopy by Barry R. Masters Pdf

This book presents a comprehensive and coherent summary of techniques for enhancing the resolution and image contrast provided by far-field optical microscopes. It takes a critical look at the body of knowledge that comprises optical microscopy, compares and contrasts the various instruments, provides a clear discussion of the physical principles that underpin these techniques, and describes advances in science and medicine for which superresolution microscopes are required and are making major contributions. The text fills significant gaps that exist in other works on superresolution imaging, firstly by placing a new emphasis on the specimen, a critical component of the microscope setup, giving equal importance to the enhancement of both resolution and contrast. Secondly, it covers several topics not typically discussed in depth, such as Bessel and Airy beams, the physics of the spiral phase plate, vortex beams and singular optics, photoactivated localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM), and light-sheet fluorescence microscopy (LSFM). Several variants of these techniques are critically discussed. Noise, optical aberrations, specimen damage, and artifacts in microscopy are also covered. The importance of validation of superresolution images with electron microscopy is stressed. Additionally, the book includes translations and discussion of seminal papers by Abbe and Helmholtz that proved to be pedagogically relevant as well as historically significant. This book is written for students, researchers, and engineers in the life sciences, medicine, biological engineering, and materials science who plan to work with or already are working with superresolution light microscopes. The volume can serve as a reference for these areas while a selected set of individual chapters can be used as a textbook for a one-semester undergraduate or first-year graduate course on superresolution microscopy. Moreover, the text provides a captivating account of curiosity, skepticism, risk-taking, innovation, and creativity in science and technology. Good scientific practice is emphasized throughout, and the author’s lecture slides on responsible conduct of research are included as an online resource which will be of interest to students, course instructors, and scientists alike.

Functional Imaging by Controlled Nonlinear Optical Phenomena

Author : Kazuyoshi Itoh,Keisuke Isobe,Wataru Watanabe
Publisher : John Wiley & Sons
Page : 286 pages
File Size : 50,7 Mb
Release : 2013-09-03
Category : Science
ISBN : 9781118751770

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Functional Imaging by Controlled Nonlinear Optical Phenomena by Kazuyoshi Itoh,Keisuke Isobe,Wataru Watanabe Pdf

Ultrafast lasers allow high-precision imaging and manipulation for biological and medical applications. Nonlinear optical microscopy has provided researchers with unique possibilities of three-dimensional imaging of biological cells and tissues. Nonlinear optical imaging technique is a rapidly emerging research area with widespread fundamental research and clinical applications. Nonlinear optical imaging allows both structural and functional imaging with cellular level resolution imaging in biological systems. The introduction of endogenous or exogenous probes can selectively enhance contrast for molecular targets in a living cell as well as supply functional information on processes. With the aim to control nonlinear optical processes and to obtain functional images, nonlinear optical processes can be controlled by photo-controlled probes and/or parameters of ultrafast laser pulses, such as time, space, polarization, and phase. This book gives an overview of the nonlinear optical process by ultrafast laser pulses and explains how the basics of nonlinear optical microscopy led to the most advanced techniques of photo-controlled nonlinear optical microscopy.

A Practical Guide to Optical Microscopy

Author : John Girkin
Publisher : CRC Press
Page : 247 pages
File Size : 55,7 Mb
Release : 2019-06-14
Category : Science
ISBN : 9781351630351

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A Practical Guide to Optical Microscopy by John Girkin Pdf

Choice Recommended Title, March 2020 Optical microscopy is used in a vast range of applications ranging from materials engineering to in vivo observations and clinical diagnosis, and thanks to the latest advances in technology, there has been a rapid growth in the number of methods available. This book is aimed at providing users with a practical guide to help them select, and then use, the most suitable method for their application. It explores the principles behind the different forms of optical microscopy, without the use of complex maths, to provide an understanding to help the reader utilise a specific method and then interpret the results. Detailed physics is provided in boxed sections, which can be bypassed by the non-specialist. It is an invaluable tool for use within research groups and laboratories in the life and physical sciences, acting as a first source for practical information to guide less experienced users (or those new to a particular methodology) on the range of techniques available. Features: The first book to cover all current optical microscopy methods for practical applications Written to be understood by a non-optical expert with inserts to provide the physical science background Brings together conventional widefield and confocal microscopy, with advanced non-linear and super resolution methods, in one book To learn more about the author please visit here.

Optical Imaging and Microscopy

Author : Peter Török,Fu-Jen Kao
Publisher : Springer
Page : 402 pages
File Size : 49,9 Mb
Release : 2013-06-05
Category : Science
ISBN : 9783540460220

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Optical Imaging and Microscopy by Peter Török,Fu-Jen Kao Pdf

This text draws together the fields of optical microscopy and optical data storage, in a unique compilation of valuable and novel scientific work that is scarcely to be found elsewhere. The contributing authors are unquestioned leaders of their respective fields.

Introduction to Optical Microscopy

Author : Jerome Mertz
Publisher : Cambridge University Press
Page : 475 pages
File Size : 48,8 Mb
Release : 2019-08
Category : Medical
ISBN : 9781108428309

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Introduction to Optical Microscopy by Jerome Mertz Pdf

Presents a fully updated, self-contained textbook covering the core theory and practice of both classical and modern optical microscopy techniques.

Quantitative Biomedical Optics

Author : Irving J. Bigio,Sergio Fantini
Publisher : Cambridge University Press
Page : 703 pages
File Size : 49,9 Mb
Release : 2016-01-07
Category : Science
ISBN : 9780521876568

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Quantitative Biomedical Optics by Irving J. Bigio,Sergio Fantini Pdf

Based on physical science principles, Quantitative Biomedical Optics covers theory, instrumentation, methods and applications, with practical exercises and problem sets.

Mechanobiology Handbook, Second Edition

Author : Jiro Nagatomi,Eno Essien Ebong
Publisher : CRC Press
Page : 705 pages
File Size : 48,5 Mb
Release : 2018-12-07
Category : Medical
ISBN : 9780429816741

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Mechanobiology Handbook, Second Edition by Jiro Nagatomi,Eno Essien Ebong Pdf

Mechanobiology—the study of the effects of mechanics on biological events—has evolved to answer numerous research questions. Mechanobiology Handbook 2nd Edition is a reference book for engineers, scientists, and clinicians who are interested in mechanobiology and a textbook for senior undergraduate to graduate level students of this growing field. Readers will gain a comprehensive review of recent research findings as well as elementary chapters on solid mechanics, fluid mechanics, and molecular analysis techniques. The new edition presents, in addition to the chapters of the first edition, homework problem sets that are available online and reviews of research in uncovered areas. Moreover, the new edition includes chapters on statistical analysis, design of experiments and optical imaging. The editors of this book are researchers and educators in mechanobiology. They realized a need for a single volume to assist course instructors as a guide for didactic teaching of mechanobiology to a diverse student body. A mechanobiology course is frequently made up of both undergraduate and graduate students pursuing degrees in engineering, biology, or integrated engineering and biology. Their goal was to present both the elementary and cutting-edge aspects of mechanobiology in a manner that is accessible to students from many different academic levels and from various disciplinary backgrounds. Moreover, it is their hope that the readers of Mechanobiology Handbook 2nd Edition will find study questions at the end of each chapter useful for long-term learning and further discussion. Comprehensive collection of reviews of recent research Introductory materials in mechanics, biology, and statistics Discussion of pioneering and emerging mechanobiology concepts Presentation of cutting-edge mechanobiology research findings across various fields and organ systems End of chapter study questions, available online Considering the complexity of the mechanics and the biology of the human body, most of the world of mechanobiology remains to be studied. Since the field is still developing, the Mechanobiology Handbook raises many different viewpoints and approaches with the intention of stimulating further research endeavours.

Biomedical Optical Phase Microscopy and Nanoscopy

Author : Natan T. Shaked,Zeev Zalevsky,Lisa L Satterwhite
Publisher : Academic Press
Page : 428 pages
File Size : 51,5 Mb
Release : 2012-11-05
Category : Medical
ISBN : 9780124158719

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Biomedical Optical Phase Microscopy and Nanoscopy by Natan T. Shaked,Zeev Zalevsky,Lisa L Satterwhite Pdf

Written by leading optical phase microscopy experts, this book is a comprehensive reference to phase microscopy and nanoscopy techniques for biomedical applications, including differential interference contrast (DIC) microscopy, phase contrast microscopy, digital holographic microscopy, optical coherence tomography, tomographic phase microscopy, spectral-domain phase detection, and nanoparticle usage for phase nanoscopy The Editors show biomedical and optical engineers how to use phase microscopy for visualizing unstained specimens, and support the theoretical coverage with applied content and examples on designing systems and interpreting results in bio- and nanoscience applications. Provides a comprehensive overview of the principles and techniques of optical phase microscopy and nanoscopy with biomedical applications. Tips/advice on building systems and working with advanced imaging biomedical techniques, including interpretation of phase images, and techniques for quantitative analysis based on phase microscopy. Interdisciplinary approach that combines optical engineering, nanotechnology, biology and medical aspects of this topic. Each chapter includes practical implementations and worked examples.

Second Harmonic Generation Imaging

Author : Francesco S. Pavone,Paul J. Campagnola
Publisher : Taylor & Francis
Page : 465 pages
File Size : 46,8 Mb
Release : 2016-04-19
Category : Science
ISBN : 9781439849156

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Second Harmonic Generation Imaging by Francesco S. Pavone,Paul J. Campagnola Pdf

Second-harmonic generation (SHG) microscopy has shown great promise for imaging live cells and tissues, with applications in basic science, medical research, and tissue engineering. Second Harmonic Generation Imaging offers a complete guide to this optical modality, from basic principles, instrumentation, methods, and image analysis to biomedical a

Biomedical Optical Imaging

Author : James G. Fujimoto,Daniel Farkas
Publisher : Oxford University Press
Page : 440 pages
File Size : 47,5 Mb
Release : 2009-04-22
Category : Science
ISBN : 0199722293

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Biomedical Optical Imaging by James G. Fujimoto,Daniel Farkas Pdf

Biomedical optical imaging is a rapidly emerging research area with widespread fundamental research and clinical applications. This book gives an overview of biomedical optical imaging with contributions from leading international research groups who have pioneered many of these techniques and applications. A unique research field spanning the microscopic to the macroscopic, biomedical optical imaging allows both structural and functional imaging. Techniques such as confocal and multiphoton microscopy provide cellular level resolution imaging in biological systems. The integration of this technology with exogenous chromophores can selectively enhance contrast for molecular targets as well as supply functional information on processes such as nerve transduction. Novel techniques integrate microscopy with state-of-the-art optics technology, and these include spectral imaging, two photon fluorescence correlation, nonlinear nanoscopy; optical coherence tomography techniques allow functional, dynamic, nanoscale, and cross-sectional visualization. Moving to the macroscopic scale, spectroscopic assessment and imaging methods such as fluorescence and light scattering can provide diagnostics of tissue pathology including neoplastic changes. Techniques using light diffusion and photon migration are a means to explore processes which occur deep inside biological tissues and organs. The integration of these techniques with exogenous probes enables molecular specific sensitivity.

Microscopy and Analysis

Author : Stefan G. Stanciu
Publisher : BoD – Books on Demand
Page : 444 pages
File Size : 45,7 Mb
Release : 2016-09-21
Category : Technology & Engineering
ISBN : 9789535125785

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Microscopy and Analysis by Stefan G. Stanciu Pdf

Microscopes represent tools of the utmost importance for a wide range of disciplines. Without them, it would have been impossible to stand where we stand today in terms of understanding the structure and functions of organelles and cells, tissue composition and metabolism, or the causes behind various pathologies and their progression. Our knowledge on basic and advanced materials is also intimately intertwined to the realm of microscopy, and progress in key fields of micro- and nanotechnologies critically depends on high-resolution imaging systems. This volume includes a series of chapters that address highly significant scientific subjects from diverse areas of microscopy and analysis. Authoritative voices in their fields present in this volume their work or review recent trends, concepts, and applications, in a manner that is accessible to a broad readership audience from both within and outside their specialist area.

Recent Advances in Scar Biology

Author : Rei Ogawa
Publisher : MDPI
Page : 203 pages
File Size : 42,6 Mb
Release : 2019-01-29
Category : Biology (General)
ISBN : 9783038973980

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Recent Advances in Scar Biology by Rei Ogawa Pdf

This book is a printed edition of the Special Issue "Recent Advances in Scar Biology" that was published in IJMS