Handbook Of Molecular And Cellular Methods In Biology And Medicine Second Edition

Author : Leland J. Cseke,Peter B. Kaufman,Gopi K. Podila,Chung-Jui Tsai
Publisher : CRC Press
Page : 604 pages
File Size : 48,8 Mb
Release : 2003-11-24
Category : Science
ISBN : 0849308151

Get Book

Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition by Leland J. Cseke,Peter B. Kaufman,Gopi K. Podila,Chung-Jui Tsai Pdf

Since the publication of the best-selling Handbook of Molecular and Cellular Methods in Biology and Medicine, the field of biology has experienced several milestones. Genome sequencing of higher eukaryotes has progressed at an unprecedented speed. Starting with baker's yeast (Saccharomyces cerevisiae), organisms sequenced now include human (Homo sapiens), model crucifer (Arabidopsis thaliana), and rice (Oryza sativa). The invention of DNA microarray technology and advances in bioinformatics have generated vast amounts of genomic data. Reflecting these revolutionary advances Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition documents conventional and modern approaches to tackle scientific research in the post-genomics era. Maintaining the step-by-step format that popularized the first edition, each chapter provides the principles behind the featured method, a detailed description of each protocol, applications of the protocol to different systems, and references for further study. Handbook of Molecular and Cellular Methods in Biology and Medicine, Second Edition now includes: New protocols in all chapters, including alternative protocols In vitro transcription methods Analysis of DNA sequences New bioseparation techniques New chapters covering: mRNA differential display Inhibition of gene expression In situ hybridization (Localization of gene expression) Combinatorial techniques Computational data mining methods applied to combinatorial chemistry libraries With this book at hand, researchers, teachers, and students can understand and utilize the major techniques and methods currently employed in cellular and molecular biology.

Author : Anonim
Publisher : Unknown
Page : 580 pages
File Size : 45,8 Mb
Release : 2004
Category : Cytology
ISBN : 0429214561

Get Book

Handbook of Molecular and Cellular Methods in Biology and Medicine by Anonim Pdf

Author : Leland J. Cseke,Ara Kirakosyan,Peter B. Kaufman,Margaret V. Westfall
Publisher : CRC Press
Page : 739 pages
File Size : 50,7 Mb
Release : 2011-12-12
Category : Science
ISBN : 9781420069389

Get Book

Handbook of Molecular and Cellular Methods in Biology and Medicine, Third Edition by Leland J. Cseke,Ara Kirakosyan,Peter B. Kaufman,Margaret V. Westfall Pdf

Several milestones in biology have been achieved since the first publication of the Handbook of Molecular and Cellular Methods in Biology and Medicine. This is true particularly with respect to genome-level sequencing of higher eukaryotes, the invention of DNA microarray technology, advances in bioinformatics, and the development of RNAi technology. Now in its third edition, this volume provides researchers with an updated tool kit that incorporates conventional as well as modern approaches to tackle biological and medicinal research in the post-genomics era. Significantly revised to address these recent changes, the editors have evaluated, revised, and sometimes replaced protocols with more efficient, more reliable, or simpler ones. The book has also been reorganized with section headings focusing on different biological levels connected to one another, taking into account the central dogma of biology (DNA → RNA → protein → metabolites). The book first explores traditional approaches and then moves to the modern "omics" approaches, including genomics, proteomics, and metabolomics. It also discusses the manipulation of biological systems (including RNAi) and macromolecular analyses, focusing on the use of microscopy. In each chapter, various notes and cautionary considerations are presented for potentially hazardous reagents. Filled with diagrams, tables, and figures to clarify methods, most chapters also contain Troubleshooting Guides indicating problems, possible causes, and solutions that may be incurred in carrying out the procedures. Researchers and scientists who master the techniques in this book are putting themselves at the cutting edge of biological and medicinal research.

Author : Donghern Kim,Leland J. Cseke,Peter B. Kaufman,William Wu
Publisher : CRC Press
Page : 496 pages
File Size : 48,9 Mb
Release : 2004-11-23
Category : Science
ISBN : 1135517258

Get Book

Handbook of Molecular and Cellular Methods in Biology and Medicine by Donghern Kim,Leland J. Cseke,Peter B. Kaufman,William Wu Pdf

Many of the available books in cellular and molecular biology fail to explain the principles and methods described. Handbook of Molecular and Cellular Methods in Biology and Medicine fills in these gaps. Not only do the authors describe the many methods and techniques most commonly used, but they also discuss why you would use each particular method. This handbook covers a wide range of techniques and methods that have been developed and extensively tested. Described in a logical framework of 21 chapters, methods include isolation and purification of DNA, RNA, and proteins; preparation of hybridization probes; electrophoresis and blotting techniques; construction of cDNA and genomic DNA libraries; DNA sequencing; PCR technology; DNA recombination and mutagenesis; transcription in vitro and translation in vitro; gene transfer and analysis of gene expression in transgenic animals and plants; cell and tissue cultures; and microscopy, as well as bioseparation techniques and preparation of monoclonal and polyclonal antibodies. The authors provide descriptive illustrations for each method. Step-by-step instructions allow you to use this book in the lab while performing the various techniques. Each chapter provides the principles behind the method, easy-to-follow instructions, the reagents and materials required, and references for further study. Many chapters also include a troubleshooting guide, cautioning what to avoid and indicating steps that require extra care. Every biologist needs this book on their lab bench!

Author : Donghern Kim,Peter B. Kaufman,Leland J. Cseke,William Wu
Publisher : CRC Press
Page : 496 pages
File Size : 43,7 Mb
Release : 1995-05-15
Category : Science
ISBN : 0849325110

Get Book

Handbook of Molecular and Cellular Methods in Biology and Medicine by Donghern Kim,Peter B. Kaufman,Leland J. Cseke,William Wu Pdf

Many of the available books in cellular and molecular biology fail to explain the principles and methods described. Handbook of Molecular and Cellular Methods in Biology and Medicine fills in these gaps. Not only do the authors describe the many methods and techniques most commonly used, but they also discuss why you would use each particular method. This handbook covers a wide range of techniques and methods that have been developed and extensively tested. Described in a logical framework of 21 chapters, methods include isolation and purification of DNA, RNA, and proteins; preparation of hybridization probes; electrophoresis and blotting techniques; construction of cDNA and genomic DNA libraries; DNA sequencing; PCR technology; DNA recombination and mutagenesis; transcription in vitro and translation in vitro; gene transfer and analysis of gene expression in transgenic animals and plants; cell and tissue cultures; and microscopy, as well as bioseparation techniques and preparation of monoclonal and polyclonal antibodies. The authors provide descriptive illustrations for each method. Step-by-step instructions allow you to use this book in the lab while performing the various techniques. Each chapter provides the principles behind the method, easy-to-follow instructions, the reagents and materials required, and references for further study. Many chapters also include a troubleshooting guide, cautioning what to avoid and indicating steps that require extra care. Every biologist needs this book on their lab bench!

Author : Leland J. Cseke,Ara Kirakosyan,Peter B. Kaufman,Margaret V. Westfall
Publisher : CRC Press
Page : 735 pages
File Size : 46,6 Mb
Release : 2016-04-19
Category : Medical
ISBN : 9781439881958

Get Book

Handbook of Molecular and Cellular Methods in Biology and Medicine by Leland J. Cseke,Ara Kirakosyan,Peter B. Kaufman,Margaret V. Westfall Pdf

Several milestones in biology have been achieved since the first publication of the Handbook of Molecular and Cellular Methods in Biology and Medicine. This is true particularly with respect to genome-level sequencing of higher eukaryotes, the invention of DNA microarray technology, advances in bioinformatics, and the development of RNAi technology

Author : John M. Walker,Ralph Rapley
Publisher : Springer Science & Business Media
Page : 1105 pages
File Size : 51,8 Mb
Release : 2008-11-04
Category : Science
ISBN : 9781603273756

Get Book

Molecular Biomethods Handbook by John M. Walker,Ralph Rapley Pdf

Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. This book expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Written by a well-established panel of research scientists, the book provides an up-to-date collection of methods used regularly in the authors’ own research programs.

Author : Rene Fester Kratz
Publisher : John Wiley & Sons
Page : 384 pages
File Size : 49,7 Mb
Release : 2009-06-02
Category : Science
ISBN : 9780470430668

Get Book

Molecular and Cell Biology For Dummies by Rene Fester Kratz Pdf

Your hands-on study guide to the inner world of the cell Need to get a handle on molecular and cell biology? This easy-to-understand guide explains the structure and function of the cell and how recombinant DNA technology is changing the face of science and medicine. You discover how fundamental principles and concepts relate to everyday life. Plus, you get plenty of study tips to improve your grades and score higher on exams! Explore the world of the cell — take a tour inside the structure and function of cells and see how viruses attack and destroy them Understand the stuff of life (molecules) — get up to speed on the structure of atoms, types of bonds, carbohydrates, proteins, DNA, RNA, and lipids Watch as cells function and reproduce — see how cells communicate, obtain matter and energy, and copy themselves for growth, repair, and reproduction Make sense of genetics — learn how parental cells organize their DNA during sexual reproduction and how scientists can predict inheritance patterns Decode a cell's underlying programming — examine how DNA is read by cells, how it determines the traits of organisms, and how it's regulated by the cell Harness the power of DNA — discover how scientists use molecular biology to explore genomes and solve current world problems Open the book and find: Easy-to-follow explanations of key topics The life of a cell — what it needs to survive and reproduce Why molecules are so vital to cells Rules that govern cell behavior Laws of thermodynamics and cellular work The principles of Mendelian genetics Useful Web sites Important events in the development of DNA technology Ten great ways to improve your biology grade

Author : Robert Wilkins,Simon Cross,Ian Megson,David Meredith
Publisher : OUP Oxford
Page : 990 pages
File Size : 48,9 Mb
Release : 2011-09-22
Category : Medical
ISBN : 9780191652295

Get Book

Oxford Handbook of Medical Sciences by Robert Wilkins,Simon Cross,Ian Megson,David Meredith Pdf

Written by biomedical scientists and clinicians, with the purpose of disseminating the fundamental scientific principles that underpin medicine, this new edition of the Oxford Handbook of Medical Sciences provides a clear, easily digestible account of basic cell physiology and biochemistry. It also includes an investigation of the traditional pillars of medicine (anatomy, physiology, biochemistry, pathology and pharmacology) integrated in the context of each of the major systems relevant to the human body. Cross-referenced to the Oxford Handbook of Clinical Medicine, and thoroughly illustrated, it is the ideal introduction to the medical sciences for medical students and biomedical scientists, as well as a valuable refresher for junior doctors.

Author : Trygve O Tollefsbol
Publisher : Academic Press
Page : 920 pages
File Size : 43,5 Mb
Release : 2022-09-08
Category : Science
ISBN : 9780323919500

Get Book

Handbook of Epigenetics by Trygve O Tollefsbol Pdf

Handbook of Epigenetics: The New Molecular and Medical Genetics, Third Edition provides a comprehensive analysis of epigenetics, from basic biology to clinical application. This new edition has been fully revised to cover the latest and evolving topics in epigenetics, with chapters updated and new chapters added on topics such as single-cell epigenetics, DNA methylation clocks in age-related diseases, transposable elements and epigenetics, X chromosome inactivation, and the epigenetics of drug addiction, among other topics. Throughout this edition, greater emphasis falls on epigenomic analyses and incorporating multi-omics approaches rather than gene-specific analyses. In addition, this edition has also been enhanced with step-by-step instructions in research methods, as well as easy-to-digest disease case studies and clinical trials that provide context and applied examples of recent advances in disease understanding and epigenetic therapeutics. These features empower researchers to reproduce the approaches and studies discussed and aid clinical translation. Live links across chapters tie in relevant external datasets and resources. Provides a timely and comprehensive collection of fully up-to-date coverage of epigenetics Covers basic epigenetic biology, research methods and technology, disease relationships and clinical medicine Written at a verbal and technical level that can be understood by scientists and students alike, with chapter summaries and conclusions included throughout Discusses exciting new topics in epigenetics, such as DNA methylation clocks in age-related diseases, transposable elements and epigenetics, X chromosome inactivation, and the epigenetics of drug addiction Includes step-by-step instructions in research protocols to aid reproducibility, as well as easy-to-digest disease case studies and clinical trials, providing context and applied examples of recent clinical translation

Author : Pei-Show Juo
Publisher : CRC Press
Page : 1163 pages
File Size : 55,6 Mb
Release : 2001-12-21
Category : Medical
ISBN : 9781420041309

Get Book

Concise Dictionary of Biomedicine and Molecular Biology by Pei-Show Juo Pdf

Rapid advances in science, medicine, and molecular biology have created a large amount of new information on biomedicine and molecular biology. Keeping up with the latest information can become a cumbersome task for professionals and students working in these fields. Updated to include new terminology and accurate characterizations of previously ex

Author : Gary H. Perdew,Jack P. Vanden Heuvel,Jeffrey M. Peters
Publisher : Springer Science & Business Media
Page : 334 pages
File Size : 55,5 Mb
Release : 2008-08-17
Category : Science
ISBN : 9781597452281

Get Book

Regulation of Gene Expression by Gary H. Perdew,Jack P. Vanden Heuvel,Jeffrey M. Peters Pdf

The use of molecular biology and biochemistry to study the regulation of gene expression has become a major feature of research in the biological sciences. Many excellent books and reviews exist that examine the experimental methodology employed in specific areas of molecular biology and regulation of gene expression. However, we have noticed a lack of books, especially textbooks, that provide an overview of the rationale and general experimental approaches used to examine chemically or disease-mediated alterations in gene expression in mammalian systems. For example, it has been difficult to find appropriate texts that examine specific experimental goals, such as proving that an increased level of mRNA for a given gene is attributable to an increase in transcription rates. Regulation of Gene Expression: Molecular Mechanisms is intended to serve as either a textbook for graduate students or as a basic reference for laboratory personnel. Indeed, we are using this book to teach a graduate-level class at The Pennsylvania State University. For more details about this class, please visit http://moltox. cas. psu. edu and select “Courses. ” The goal for our work is to provide an overview of the various methods and approaches to characterize possible mechanisms of gene regulation. Further, we have attempted to provide a framework for students to develop an understanding of how to determine the various mechanisms that lead to altered activity of a specific protein within a cell.

Author : Giorgio Stanta
Publisher : Springer Science & Business Media
Page : 301 pages
File Size : 55,9 Mb
Release : 2011-06-06
Category : Medical
ISBN : 9783642178900

Get Book

Guidelines for Molecular Analysis in Archive Tissues by Giorgio Stanta Pdf

A huge amount of fixed and paraffin-embedded tissue is stored in every hospital. This is very precious material that can be used for translational research and for diagnostics. The molecular methods employed for analysis of these tissues are similar to the usual molecular biology and proteomics methods, but reliable results can be obtained only if specific steps are followed with great care. This book provides detailed and precise guidelines for molecular analysis of archive tissues and will serve as an invaluable aid for researchers and pathologists involved in translational research and diagnostics. Clear notes and explanations are included to simplify use of the protocols for the less experienced. The authors are a group of acknowledged experts who have developed the described methods and validated them within the European project "Archive Tissues: Improving Molecular Medicine Research and Clinical Practice - IMPACTS", which has involved 21 leading institutions in 11 countries.

Author : William Wu,Helen H. Zhang,Michael J. Welsh,Peter B. Kaufman
Publisher : CRC Press
Page : 536 pages
File Size : 55,6 Mb
Release : 2003-09-29
Category : Medical
ISBN : 9780203489277

Get Book

Gene Biotechnology by William Wu,Helen H. Zhang,Michael J. Welsh,Peter B. Kaufman Pdf

Many scientists find themselves working in the laboratory without sufficient background in current biotechnology methods. Others want to keep up with the revolution in biotechnology and the flood of new methodologies. This book provides a solution for both: a multidisciplinary approach to the methods essential to biotechnical development. C

Author : Akash Gautam
Publisher : Springer Nature
Page : 203 pages
File Size : 41,6 Mb
Release : 2022-03-29
Category : Science
ISBN : 9783030942304

Get Book

DNA and RNA Isolation Techniques for Non-Experts by Akash Gautam Pdf

This thorough introductory volume presents the background, applications, and stepwise directions for standard DNA and RNA isolation techniques. Unlike a kit chemistry approach, this book provides a breadth of information necessary for junior or non-expert researchers to learn and apply these techniques in their work. An accessible, indispensable how-to guide for researchers in immunology, molecular biology, zoology, forensic science, genetics, botany, neuroscience, physiology, and others.