High Performance Liquid Chromatography Of Peptides And Proteins

Author : Marie-Isabel Aguilar
Publisher : Springer Science & Business Media
Page : 411 pages
File Size : 50,5 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592597420

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HPLC of Peptides and Proteins by Marie-Isabel Aguilar Pdf

The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.

Author : Colin T. Mant,Robert S. Hodges
Publisher : CRC Press
Page : 960 pages
File Size : 42,8 Mb
Release : 2017-11-22
Category : Medical
ISBN : 9781351440752

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High-Performance Liquid Chromatography of Peptides and Proteins by Colin T. Mant,Robert S. Hodges Pdf

This book consists of a series of 82 precise, easy-to-read articles by internationally renowned scientists and emphasizes the practical approach to HPLC with minimal theory, although the underlying principles for peptide and protein separations are clearly expressed. All of the major modes of microbore, ultrafast and analytical HPLC are discussed, including size-exclusion, ion-exchange, reversed-phase, hydrophobic interaction, and affinity and immunoaffinity chromatography. A section on preparative HPLC, including displacement techniques, is also presented. Problem-solving approaches to the separation of various classes of biologically active peptides and proteins are thoroughly explored, while the importance of peptide standards for monitoring column performance and for optimizing separation conditions is emphasized. Several articles focus on the choice of the correct detection method (electrochemical, UV, fluorescence), as well as the need for a proper knowledge of approaches to column and instrument maintenance and trouble-shooting. A section on predictive approaches deals with both computer simulation of peptide separations and peptide structure. The book also includes complementary techniques to HPLC, as well as other useful applications of HPLC. It enables both novice and experienced chromatographers to realize the full potential of this extremely powerful technique, in the process making an important contribution to scientific literature.

Author : Milton Hearn
Publisher : Elsevier
Page : 284 pages
File Size : 42,9 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323157704

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High-Performance Liquid Chromatography of Proteins and Peptides by Milton Hearn Pdf

High-Performance Liquid Chromatography of Proteins and Peptides contains the proceedings of the first International Symposium on High-Performance Liquid Chromatography of Proteins and Peptides, held in Washington, D.C., on November 16-17, 1981. The symposium focused on the use of high-performance liquid chromatography (HPLC) in the analysis, characterization, and isolation of peptides and proteins and encompassed six sessions covering size exclusion, ion exchange, and reversed phase chromatography, as well as the use of high-performance liquid chromatography (HPLC) in protein structural studies and peptide isolation. This book is comprised of 28 chapters and begins with a discussion on the status of high-performance ion-exchange chromatography of proteins, followed by an analysis of peptic fragmentation of human immunoglobulin G using HPLC. The physicochemical basis of peptide retention with chemically bonded hydrocarbonaceous silicas and the isolation of biologically active peptides from tissue extracts are also examined. Subsequent chapters explore some additional applications of HPLC, such as cord blood screening for hemoglobin disorders; purification of commercial trypsin and chymotrypsin; characterization of human alcohol dehydrogenase isoenzymes; and structural studies of neurophysins, photolabeled derivatives, and biosynthetic precursors. This monograph should be of value to students and researchers interested in the use of HPLC to study proteins and peptides.

Author : Colin T. Mant,Robert S. Hodges
Publisher : CRC Press
Page : 1250 pages
File Size : 47,7 Mb
Release : 2017-11-22
Category : Medical
ISBN : 9781351440745

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High-Performance Liquid Chromatography of Peptides and Proteins by Colin T. Mant,Robert S. Hodges Pdf

This book consists of a series of 82 precise, easy-to-read articles by internationally renowned scientists and emphasizes the practical approach to HPLC with minimal theory, although the underlying principles for peptide and protein separations are clearly expressed. All of the major modes of microbore, ultrafast and analytical HPLC are discussed, including size-exclusion, ion-exchange, reversed-phase, hydrophobic interaction, and affinity and immunoaffinity chromatography. A section on preparative HPLC, including displacement techniques, is also presented. Problem-solving approaches to the separation of various classes of biologically active peptides and proteins are thoroughly explored, while the importance of peptide standards for monitoring column performance and for optimizing separation conditions is emphasized. Several articles focus on the choice of the correct detection method (electrochemical, UV, fluorescence), as well as the need for a proper knowledge of approaches to column and instrument maintenance and trouble-shooting. A section on predictive approaches deals with both computer simulation of peptide separations and peptide structure. The book also includes complementary techniques to HPLC, as well as other useful applications of HPLC. It enables both novice and experienced chromatographers to realize the full potential of this extremely powerful technique, in the process making an important contribution to scientific literature.

Author : Anonim
Publisher : Unknown
Page : 128 pages
File Size : 52,7 Mb
Release : 1983
Category : Electronic
ISBN : OCLC:664589267

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High-performance Liquid Chromatography of Proteins and Peptides by Anonim Pdf

Author : William S. Hancock
Publisher : CRC-Press
Page : 514 pages
File Size : 47,7 Mb
Release : 1984-07-02
Category : Science
ISBN : 0849335108

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HDBK HPLC FOR SEPARATION AMINO ACIDS PEPTIDES & PROTEINS by William S. Hancock Pdf

Introduction to the general principles of HPLC; HPLC instrumentation; Mobile phases; Detection methods; Separation of free amino acids; Resolution of amino acids as diastereomeric derivatives.

Author : William S. Hancock
Publisher : CRC-Press
Page : 544 pages
File Size : 52,5 Mb
Release : 1984-08-14
Category : Science
ISBN : 0849335116

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Handbook HPLC for Separation Amino Acids Peptides and Proteins by William S. Hancock Pdf

All areas of high performance liquid chromatography (HPLC) relevant to the separation of amino acid and polypeptide samples are described in these comprehensive volumes. Eighty-four chapters cover the application of HPLC to research in the life sciences. Both basic information and specific details are presented on such areas as commercially available HPLC columns, ligand-exchange chromatography, peptide hormone separations, peptide antibiotic separations and drug-protein binding. Practical suggestions are given to help you avoid pitfalls which can exist in the analysis of biological materials.

Author : Kato,Simone Parvez,Hasan Parvez
Publisher : CRC Press
Page : 258 pages
File Size : 45,5 Mb
Release : 2020-03-24
Category : Science
ISBN : 9781000083170

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Gel Permeation and Ion-Exchange Chromatography of Proteins and Peptides by Kato,Simone Parvez,Hasan Parvez Pdf

This book is a collection of critical reviews of the use of high-performance liquid chromatography in a very specialized area of research. It describes in detail modern methodology to separate nucleic acids, enzymes and a wide variety of biologically active proteins such as renin.

Author : Taylor & Francis Group
Publisher : Unknown
Page : 128 pages
File Size : 41,7 Mb
Release : 2018-09-30
Category : Electronic
ISBN : 1315891972

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Crc Handbook of HPLC for the Separation of Amino Acids Peptides and Proteins by Taylor & Francis Group Pdf

Author : Karen M. Gooding,Fred E. Regnier
Publisher : CRC Press
Page : 791 pages
File Size : 49,9 Mb
Release : 2002-01-08
Category : Science
ISBN : 9780203911594

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Hplc Of Biological Macro- Molecules, Revised And Expanded by Karen M. Gooding,Fred E. Regnier Pdf

Completely revised to reflect the innovations in HPLC from the past decade, this authoritative reference presents practical strategies for the evaluation and analysis of proteins, peptides, and polynucleotides. Offering class-specific applications for the characterization and fractionation of biological macromolecules, the book contains material on organic supports, size exclusion, ion exchange, hydrophobic interaction, and metal interaction chromatography. Leading experts summarize specialized detection systems, provides discussions on the chemical and biological properties of specific biomolecules, include detailed guidelines for the development of analytical techniques, and more.

Author : M. Kastner
Publisher : Elsevier
Page : 978 pages
File Size : 47,8 Mb
Release : 2000
Category : Science
ISBN : 0444502114

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Protein Liquid Chromatography by M. Kastner Pdf

Protein Liquid Chromatography is a handbook-style guide to liquid chromatography as a tool for isolating and purifying proteins, consisting of 25 individual chapters divided into three parts: Part A covers commonly-used, classic modes of chromatography such as ion-exchange, size-exclusion, and reversed-phase; Part B deals with various target protein classes such as membrane proteins, recombinant proteins, and glycoproteins; and Part C looks at various miscellaneous related topics, including coupling reaction, buffer solution additives, and software. The text as a whole can be viewed as a systematic survey of available methods and how best to use them, but also attempts to provide an exhaustive coverage of each facet. How to solve a specific problem using a chosen method is the overall essence of the volume. The principle philosophy of this compilation is that practical application is everything; therefore, both classical and modern methods are presented in detail, with examples involving conventional, medium- and high-pressure techniques. Over-exposure to history, concept, and theory has deliberately been avoided. The reader will find a wealth of tips and tricks from users for users, including advice on the advantages and disadvantages of each method. Easy-to-read sections on "Getting started now" and "Where to go from here" attempt to provide hands-on, fool-proof detailed practical procedures with complete and even standard model runs for any scientist or technician at work in this area.

Author : Milton T. W. Hearn
Publisher : Wiley-VCH
Page : 776 pages
File Size : 50,6 Mb
Release : 1991
Category : ADN - Séparation
ISBN : 0895732955

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HPLC of Proteins, Peptides and Polynucleotides by Milton T. W. Hearn Pdf

Author : Thomas Letzel
Publisher : Royal Society of Chemistry
Page : 195 pages
File Size : 49,6 Mb
Release : 2011
Category : Science
ISBN : 9781849731829

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Protein and Peptide Analysis by LC-MS by Thomas Letzel Pdf

This book is the first example in presenting LC-MS strategies for the analysis of peptides and proteins with detailed information and hints about the needs and problems described from experts on-the-job. The best advantage is -for sure- the practical insight of experienced analysts into their novel protein analysis techniques. Readers starting in 'Proteomics' should be able to repeat each experiment with own equipment and own protein samples, like clean-up, direct protein analysis, after (online) digest, with modifications and others. Furthermore, the reader will learn more about strategies in protein analysis, like quantitative analysis, industrial standards, functional analysis and more.

Author : Regnier
Publisher : Unknown
Page : 128 pages
File Size : 53,9 Mb
Release : 1997-05-01
Category : Electronic
ISBN : 0471828424

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High Performance Liquid Chromatography of Proteins and Peptides by Regnier Pdf

Author : M. T. W. Hearn
Publisher : Wiley-VCH
Page : 776 pages
File Size : 42,7 Mb
Release : 1991-08-02
Category : Science
ISBN : 047118716X

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HPLC of Proteins, Peptides and Polynucleotides by M. T. W. Hearn Pdf

A detailed examination of contemporary topics and applications of HPLC and related techniques in the analysis and purification of biomolecules. Major topics treated include: * sample and column preparation * column materials * protein interactions * preparative and purification chromatography * multimode and microscale chromatography * affinity chromatography * high performance electrophoresis This book is the second volume in the continuing series 'Analytical Techniques in Clinical Chemistry and Laboratory Medicine'.