Histochemistry Of Single Molecules

Author : Carlo Pellicciari,Marco Biggiogera,Manuela Malatesta
Publisher : Springer Nature
Page : 351 pages
File Size : 40,8 Mb
Release : 2022-09-24
Category : Science
ISBN : 9781071626757

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Histochemistry of Single Molecules by Carlo Pellicciari,Marco Biggiogera,Manuela Malatesta Pdf

This volume details histochemical techniques for the detection of specific molecules or metabolic processes, both at light and electron microscopy. Chapters are divided into seven sections covering Vital histochemistry, Carbohydrate histochemistry, Protein histochemistry, Lipid histochemistry, Nuclear histochemistry, Plant histochemistry and Histochemistry for Nanoscience. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. The volume also contains three discursive chapters on Histochemistry in advanced cytometry, Lectins and Detection of molecules in plant cell walls by fluorescence microscopy. Authoritative and cutting-edge, Histochemistry of Single Molecules: Methods and Protocols, Second Edition aims to be a useful practical guide for researchers to help further their study in this field.

Author : Erwin J. G. Peterman,Gijs J. L. Wuite
Publisher : Humana Press
Page : 317 pages
File Size : 47,5 Mb
Release : 2011-09-15
Category : Science
ISBN : 1617792810

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Single Molecule Analysis by Erwin J. G. Peterman,Gijs J. L. Wuite Pdf

Life scientists believe that life is driven, directed, and shaped by biomolecules working on their own or in concert. It is only in the last few decades that technological breakthroughs in sensitive fluorescence microscopy and single-molecule manipulation techniques have made it possible to observe and manipulate single biomolecules and measure their individual properties. The methodologies presented in Single Molecule Techniques: Methods and Protocols are being applied more and more to the study of biologically relevant molecules, such as DNA, DNA-binding proteins, and motor proteins, and are becoming commonplace in molecular biophysics, biochemistry, and molecular and cell biology. The aim of Single Molecule Techniques: Methods and Protocols is to provide a broad overview of single-molecule approaches applied to biomolecules on the basis of clear and concise protocols, including a solid introduction to the most widely used single-molecule techniques, such as optical tweezers, single-molecule fluorescence tools, atomic force microscopy, magnetic tweezers, and tethered particle motion. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Single Molecule Techniques: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and provides a broad and thorough overview of the exciting and still-emerging field of single-molecule biology.

Author : Charles Streckfus
Publisher : BoD – Books on Demand
Page : 142 pages
File Size : 44,8 Mb
Release : 2020-03-04
Category : Medical
ISBN : 9781838808204

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Immunohistochemistry by Charles Streckfus Pdf

Immunohistochemistry - The Ageless Biotechnology is a book that is ideal for undergraduate and graduate biomedical researchers, and medical and dental health professionals. It is a detailed text, which emphasizes the laboratory and clinical implications of immunohistochemistry. The text covers the advances of immunohistochemistry from its humble origins in the 1930s up to the new decade of 2020. The book also offers a review of the immunohistochemistry detection systems with emphasis on their principles, history, and their advantages. It also stipulates the limitations and delineates the factors that need to be considered for choosing an appropriate detection system for IHC applications. The book describes current laboratory techniques and new applications for the technology. As the reader will observe, the book provides new and useful information concerning the rapidly advancing field of immunohistochemistry.

Author : Z. Lojda,R. Gossrau,T.H. Schiebler
Publisher : Springer Science & Business Media
Page : 350 pages
File Size : 52,5 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9783642672347

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Enzyme Histochemistry by Z. Lojda,R. Gossrau,T.H. Schiebler Pdf

During recent years enzyme histochemical reactions have increasingly been considered as important, the reason being that enzyme histo chemistry is now a well-established link between morphology and bio chemistry. The development of numerous new methods and in particular the improvement of existing techniques contributed to the expansion of enzyme histochemical reactions. Today, the use of these methods allows detailed insight into molecular processes of single cells and their constituents. The selection of a suitable method for enzyme histochemical investigations needs thorough knowledge and critical evaluation of the reactions de scribed for the histochemical demonstration of enzymes and introduced in laboratory practice. Often, it is difficult for scientists primarily concerned with the application of methods and for laboratory assistants to comment on the value of an enzyme histochemical reaction. Our book will serve as a guide in this respect. It contains the most important histochemical methods for the localization of enzymes, all of which were checked by the authors themselves. These methods were often modified and frequently used for numerous different investigations of healthy and diseased organs in basic research and in routine practice.

Author : Jeremy Sanderson
Publisher : John Wiley & Sons
Page : 848 pages
File Size : 51,6 Mb
Release : 2019-03-04
Category : Science
ISBN : 9781118696743

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Understanding Light Microscopy by Jeremy Sanderson Pdf

Introduces readers to the enlightening world of the modern light microscope There have been rapid advances in science and technology over the last decade, and the light microscope, together with the information that it gives about the image, has changed too. Yet the fundamental principles of setting up and using a microscope rests upon unchanging physical principles that have been understood for years. This informative, practical, full-colour guide fills the gap between specialised edited texts on detailed research topics, and introductory books, which concentrate on an optical approach to the light microscope. It also provides comprehensive coverage of confocal microscopy, which has revolutionised light microscopy over the last few decades. Written to help the reader understand, set up, and use the often very expensive and complex modern research light microscope properly, Understanding Light Microscopy keeps mathematical formulae to a minimum—containing and explaining them within boxes in the text. Chapters provide in-depth coverage of basic microscope optics and design; ergonomics; illumination; diffraction and image formation; reflected-light, polarised-light, and fluorescence microscopy; deconvolution; TIRF microscopy; FRAP & FRET; super-resolution techniques; biological and materials specimen preparation; and more. Gives a didactic introduction to the light microscope Encourages readers to use advanced fluorescence and confocal microscopes within a research institute or core microscopy facility Features full-colour illustrations and workable practical protocols Understanding Light Microscopy is intended for any scientist who wishes to understand and use a modern light microscope. It is also ideal as supporting material for a formal taught course, or for individual students to learn the key aspects of light microscopy through their own study.

Author : Rudolf Rigler,H. Vogel
Publisher : Springer Science & Business Media
Page : 322 pages
File Size : 43,6 Mb
Release : 2007-12-07
Category : Technology & Engineering
ISBN : 9783540739241

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Single Molecules and Nanotechnology by Rudolf Rigler,H. Vogel Pdf

This book focuses on recent advances in the rapidly evolving field of single molecule research. These advances are of importance for the investigation of biopolymers and cellular biochemical reactions, and are essential to the development of quantitative biology. Written by leading experts in the field, the articles cover a broad range of topics, including quantum photonics of organic dyes and inorganic nanoparticles and monitoring of single molecule (enzymatic) reactions.

Author : Sijo J. Parekattil,Sandro C. Esteves,Ashok Agarwal
Publisher : Springer Nature
Page : 895 pages
File Size : 40,7 Mb
Release : 2020-01-24
Category : Health & Fitness
ISBN : 9783030323004

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Male Infertility by Sijo J. Parekattil,Sandro C. Esteves,Ashok Agarwal Pdf

A groundbreaking contribution to the literature now in its revised and expanded second edition, this textbook offers a comprehensive review of diagnostic and treatment techniques for male infertility. This state-of-the-art, evidence-based textbook incorporates new multidisciplinary and complementary medicine approaches to create a first-of-its-kind guide to treatment strategies for male infertility and beyond. While this new edition is primarily designed as a reference for students and residents in reproductive medicine and andrology, it will be equally useful as well for professionals in urology, reproductive endocrinology, embryology, and research fields who are interested in the role that antioxidants play in male infertility. World-renowned experts in these areas have been selected to participate in this work. Careful selection of the highest quality content will span the whole range of topics in the area of male infertility, providing a complete review of well-established and current diagnostic and treatment techniques for male infertility. The incorporation of 20 new chapters will enhance the book’s appeal by including the most recent advances brought to the male infertility arena. Additionally, this edition incorporates new features, including bulleted key points, review criteria and select video clips demonstrating some of the most fascinating male infertility treatment modalities. A dedicated new section on current guidelines on male infertility will enlighten readers on how to most optimally manage male infertility clinical scenarios. Covering all aspects of diagnosis and management, ART, lifestyle factors and associated conditions for male infertility, Male Infertility: Contemporary Clinical Approaches, Andrology, ART and Antioxidants will be a readily accessible, high quality reference for medical students and residents, and will be of significant value to professionals working in the various fields treating this condition as well.

Author : Chris Gell,David Brockwell,Alastair Smith
Publisher : Oxford University Press on Demand
Page : 279 pages
File Size : 54,5 Mb
Release : 2006-08-17
Category : Science
ISBN : 9780198529422

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Handbook of Single Molecule Fluorescence Spectroscopy by Chris Gell,David Brockwell,Alastair Smith Pdf

This is a practical introduction to single molecule fluorescence experiments, the analysis of the data, and applications of the techniques to the study of biological structure and function.

Author : Ann Wheeler,Ricardo Henriques
Publisher : John Wiley & Sons
Page : 307 pages
File Size : 42,7 Mb
Release : 2017-12-26
Category : Science
ISBN : 9781119096900

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Standard and Super-Resolution Bioimaging Data Analysis by Ann Wheeler,Ricardo Henriques Pdf

A comprehensive guide to the art and science of bioimaging data acquisition, processing and analysis Standard and Super-Resolution Bioimaging Data Analysis gets newcomers to bioimage data analysis quickly up to speed on the mathematics, statistics, computing hardware and acquisition technologies required to correctly process and document data. The past quarter century has seen remarkable progress in the field of light microscopy for biomedical science, with new imaging technologies coming on the market at an almost annual basis. Most of the data generated by these systems is image-based, and there is a significant increase in the content and throughput of these imaging systems. This, in turn, has resulted in a shift in the literature on biomedical research from descriptive to highly-quantitative. Standard and Super-Resolution Bioimaging Data Analysis satisfies the demand among students and research scientists for introductory guides to the tools for parsing and processing image data. Extremely well illustrated and including numerous examples, it clearly and accessibly explains what image data is and how to process and document it, as well as the current resources and standards in the field. A comprehensive guide to the tools for parsing and processing image data and the resources and industry standards for the biological and biomedical sciences Takes a practical approach to image analysis to assist scientists in ensuring scientific data are robust and reliable Covers fundamental principles in such a way as to give beginners a sound scientific base upon which to build Ideally suited for advanced students having only limited knowledge of the mathematics, statistics and computing required for image data analysis An entry-level text written for students and practitioners in the bioscience community, Standard and Super-Resolution Bioimaging Data Analysis de-mythologises the vast array of image analysis modalities which have come online over the past decade while schooling beginners in bioimaging principles, mathematics, technologies and standards.

Author : Anonim
Publisher : Academic Press
Page : 502 pages
File Size : 42,8 Mb
Release : 2016-02-26
Category : Science
ISBN : 9780128036129

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G Protein-Coupled Receptors by Anonim Pdf

G-Protein-Coupled Receptors: Signaling, Trafficking, and Regulation, a new volume in the Methods in Cell Biology series continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in G-Protein-Coupled Receptors, and includes sections on such topics signaling, trafficking and regulation. Covers the increasingly appreciated cell biology field of G-protein-coupled receptors Includes both established and new technologies Contributed by experts in the field Covers topics such as signaling, trafficking, and regulation

Author : Frank Joseph Rauscher (III)
Publisher : Cambridge University Press
Page : 985 pages
File Size : 46,5 Mb
Release : 2014
Category : Medical
ISBN : 9780521876629

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Molecular Oncology by Frank Joseph Rauscher (III) Pdf

Reviews the origins of molecular oncology, including technologies for cancer analysis, key pathways in human malignancies, and available pharmacologic therapies.

Author : Anonim
Publisher : Academic Press
Page : 372 pages
File Size : 41,6 Mb
Release : 2015-06-08
Category : Science
ISBN : 9780128033272

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Lipid Domains by Anonim Pdf

Current Topics in Membranes is targeted toward scientists and researchers in biochemistry and molecular and cellular biology, providing the necessary membrane research to assist them in discovering the current state of a particular field and in learning where that field is heading. This volume offers an up to date presentation of current knowledge in the field of Lipid Domains. Written by leading experts Contains original material, both textual and illustrative, that should become a very relevant reference material The material is presented in a very comprehensive manner Both researchers in the field and general readers should find relevant and up-to-date information

Author : John Crocker,Paul G. Murray
Publisher : John Wiley & Sons
Page : 428 pages
File Size : 46,7 Mb
Release : 2003-06-02
Category : Science
ISBN : 0470844752

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Molecular Biology in Cellular Pathology by John Crocker,Paul G. Murray Pdf

The latest edition of this highly successful text, covers the major advances in the methods used in cellular and molecular pathology. In recent years, knowledge of the molecular organization of the cell has led to the development of powerful new techniques that bring greater accuracy and objectives to the diagnosis, prognosis and management of many diseases and to the study of pathological states. This book describes the latest molecular techniques available for the analysis of diseases. In particular it includes new techniques using fluorescent dyes, DNA microarrays, protein chemistry, and mass spectrometry. It also incorporates information from the Human Genome Project, and the new disciplines of genomics and proteomics, where relevant to pathology. Color plates are a new feature of this edition, illustrating the advances in fluorescence labeling of cells.

Author : Jean-Marie Exbrayat
Publisher : CRC Press
Page : 355 pages
File Size : 40,6 Mb
Release : 2013-06-05
Category : Science
ISBN : 9781439822227

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Histochemical and Cytochemical Methods of Visualization by Jean-Marie Exbrayat Pdf

Histochemistry and cytochemistry are important fields for studying the inner workings of cells and tissues of the body. While visualization techniques have been in use for many years, new methods of detection developed recently help researchers and practitioners better understand cell activity. Histochemical and Cytochemical Methods of Visualization describes the essential techniques that can be used for histochemical investigations in both light and transmission electron microscopy. The book begins by discussing techniques in light microscopy. It reviews classical methods of visualization, histochemical and histoenzymatic methods, and methods used to visualize cell proliferation and apoptosis. Next, the book examines the cytochemical methods used in electron microscopy with traditional techniques, as well as more specialized methods. The final section provides an overview of image analysis and describes how image processing methods can be used to extract vital information. A 16-page insert supplies color illustrations to enhance the text. Techniques will continue to adapt to the latest technological innovations, allowing more and more precise quantification of images. These developments are essential to the biological as well as the medical sciences. This manual is a critical resource for novice and experienced researchers, technicians, and students who need to visualize what happens in the cell, the molecules expressed, the main enzymatic activities, and the repercussions of the molecular activities upon the structure of the cells in the body.

Author : Tadao Takeuchi
Publisher : Unknown
Page : 660 pages
File Size : 49,5 Mb
Release : 1972
Category : Cytochemistry
ISBN : NWU:35558001519962

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Histochemistry and Cytochemistry, 1972 by Tadao Takeuchi Pdf