Human Ipsc Derived Disease Models For Drug Discovery

Author : Markus H. Kuehn,Wei Zhu
Publisher : Springer Nature
Page : 331 pages
File Size : 43,5 Mb
Release : 2023-11-23
Category : Medical
ISBN : 9783031423499

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Human iPSC-derived Disease Models for Drug Discovery by Markus H. Kuehn,Wei Zhu Pdf

Since their development a decade ago, human induced pluripotent stem cells (iPSC) have revolutionized the study of human disease, given rise to regenerative medicine technologies, and provided exceptional opportunities for pharmacologic research. These cells provide an essentially unlimited supply of cell types that are difficult to obtain from patients, such as neurons or cardiomyocytes, or are difficult to maintain in primary cell culture. iPSC can be obtained from patients afflicted with a particular disease but, in combination with recently developed gene editing techniques, can also be modified to generate disease models. Moreover, the new techniques of 3 Dimensional printing and materials science facilitate the generation of organoids that can mirror organs under disease conditions. These properties make iPSC powerful tools to study how diseases develop and how they may be treated. In addition, iPSC can also be used to treat conditions in which the target cell population has been lost and such regenerative approaches hold great promise for currently untreatable diseases, including cardiac failure or photoreceptor degenerations.

Author : Haruhisa Inoue,Yukio Nakamura
Publisher : Springer
Page : 190 pages
File Size : 42,6 Mb
Release : 2019-04-01
Category : Medical
ISBN : 9789811336720

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Medical Applications of iPS Cells by Haruhisa Inoue,Yukio Nakamura Pdf

This volume highlights recent advances using iPS cells in disease modeling and medical applications along with new technologies that enhance the power of iPS cells. While the discovery of iPS cells has provided excellent opportunities for developing models of human diseases, platforms for drug discovery and cell therapies, iPS cell technology still faces many challenges. Presenting the latest advances in this rapidly evolving research area, this book is intended to widen the community of researchers and clinicians interested in the exciting field of iPS cells.

Author : Heung Jae Chun,Kwideok Park,Chun-Ho Kim,Gilson Khang
Publisher : Springer
Page : 537 pages
File Size : 50,7 Mb
Release : 2018-10-24
Category : Medical
ISBN : 9789811309472

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Novel Biomaterials for Regenerative Medicine by Heung Jae Chun,Kwideok Park,Chun-Ho Kim,Gilson Khang Pdf

This book explores in depth a wide range of new biomaterials that hold great promise for applications in regenerative medicine. The opening two sections are devoted to biomaterials designed to direct stem cell fate and regulate signaling pathways. Diverse novel functional biomaterials, including injectable nanocomposite hydrogels, electrosprayed nanoparticles, and waterborne polyurethane-based materials, are then discussed. The fourth section focuses on inorganic biomaterials, such as nanobioceramics, hydroxyapatite, and titanium dioxide. Finally, up-to-date information is provided on a wide range of smart natural biomaterials, ranging from silk fibroin-based scaffolds and collagen type I to chitosan, mussel-inspired biomaterials, and natural polymeric scaffolds. This is one of two books to be based on contributions from leading experts that were delivered at the 2018 Asia University Symposium on Biomedical Engineering in Seoul, Korea – the companion book examines in depth the latest enabling technologies for regenerative medicine.

Author : Elizabeth D. Buttermore
Publisher : Academic Press
Page : 374 pages
File Size : 46,9 Mb
Release : 2022-09-09
Category : Medical
ISBN : 9780128222782

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Phenotyping of Human iPSC-derived Neurons by Elizabeth D. Buttermore Pdf

Phenotyping of Human iPSC-derived Neurons: Patient-Driven Research examines the steps in a preclinical pipeline that utilizes iPSC-derived neuronal technology to better understand neurological disorders and identify novel therapeutics, also providing considerations and best practices. By presenting example projects that identify phenotypes and mechanisms relevant to autism spectrum disorder and epilepsy, this book allows readers to understand what considerations are important to assess at the start of project design. Sections address reproducibility issues and advances in technology at each stage of the pipeline and provide suggestions for improvement. From patient sample collection and proper controls to neuronal differentiation, phenotyping, screening, and considerations for moving to the clinic, these detailed descriptions of each stage of the pipeline will help everyone, regardless of stage in the pipeline. In recent years, drug discovery in the neurosciences has struggled to identify novel therapeutics for patients with varying indications, including epilepsy, chronic pain, and psychosis. Current treatment options for such patients are decades old and offer little relief with many side effects. One explanation for this lull in novel therapeutics is a lack of novel target identification for neurological disorders (and target identification requires exemplar preclinical data). To improve on the preclinical work that often relies on rodent modeling, the field has begun utilizing patient-derived induced pluripotent stem cells (iPSCs) to differentiate neurons in vitro for preclinical characterization of neurological disease and target identification. Discusses techniques and new technology for iPSC culturing and neuronal differentiation to establish best practices in the lab Outlines considerations for phenotypic assay development Provides information about the successes, failures, and implications of phenotyping and screening with iPSC-derived neurons Describes how human iPSC-derived neurons are being used for preclinical discovery research as well as the development of therapeutics utilizing hiPSC-derived neurons

Author : Carl-Fredrik Mandenius,James A. Ross
Publisher : Unknown
Page : 266 pages
File Size : 42,7 Mb
Release : 2019
Category : Stem cells
ISBN : 1493994778

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Cell-based Assays Using IPSCs for Drug Development and Testing by Carl-Fredrik Mandenius,James A. Ross Pdf

Author : Arlene Chiu,Mahendra S. Rao
Publisher : Springer Science & Business Media
Page : 455 pages
File Size : 54,8 Mb
Release : 2003-08-01
Category : Science
ISBN : 9781592594238

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Human Embryonic Stem Cells by Arlene Chiu,Mahendra S. Rao Pdf

A discussion of all the key issues in the use of human pluripotent stem cells for treating degenerative diseases or for replacing tissues lost from trauma. On the practical side, the topics range from the problems of deriving human embryonic stem cells and driving their differentiation along specific lineages, regulating their development into mature cells, and bringing stem cell therapy to clinical trials. Regulatory issues are addressed in discussions of the ethical debate surrounding the derivation of human embryonic stem cells and the current policies governing their use in the United States and abroad, including the rules and conditions regulating federal funding and questions of intellectual property.

Author : Uma Lakshmipathy,Mohan C Vemuri
Publisher : Humana Press
Page : 0 pages
File Size : 50,9 Mb
Release : 2013-04-02
Category : Science
ISBN : 1627033475

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Pluripotent Stem Cells by Uma Lakshmipathy,Mohan C Vemuri Pdf

Human pluripotent stem cells such as human embryonic stem cells (hESC) and induced pluripotent stem cells (iPSC) with their unique developmental plasticity hold immense potential as cellular models for drug discovery and in regenerative medicine as a source for cell replacement. While hESC are derived from a developing embryo, iPSC are generated with forced expression of key transcription factors to convert adult somatic cells to ESC-like cells, a process termed reprogramming. Using iPSC overcomes ethical issues concerning the use of developing embryos and it can be generated from patient-specific or disease-specific cells for downstream applications. Pluripotent Stem Cells: Methods and Protocols highlights the best methods and systems for the entire work flow. Divided into four convenient sections, topics include a focus on producing iPSC from diverse somatic sources, media systems for expanding ESC and iPSC with detailed protocols for directed differentiation into specific lineages, commonly used cellular and molecular characterization methods , and the potential application of labeled stem cells with specific methods for cloning, gene delivery and cell engineering. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Pluripotent Stem Cells: Methods and Protocols seeks to serve both professionals and novices with its well-honed methodologies in an effort to further our knowledge of this essential cellular feature.

Author : Michael J. Edel
Publisher : MDPI
Page : 422 pages
File Size : 40,6 Mb
Release : 2018-10-02
Category : Electronic book
ISBN : 9783038421221

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iPS Cells for Modelling and Treatment of Human Diseases by Michael J. Edel Pdf

This book is a printed edition of the Special Issue "iPS Cells for Modelling and Treatment of Human Diseases" that was published in JCM

Author : Deepak A. Lamba
Publisher : CRC Press
Page : 379 pages
File Size : 44,5 Mb
Release : 2017-04-07
Category : Medical
ISBN : 9781315402161

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Patient-Specific Stem Cells by Deepak A. Lamba Pdf

One of the biggest challenges faced in medical research had been to create accurate and relevant models of human disease. A number of good animal models have been developed to understand the pathophysiology. However, not all of them reflect the human disorder, a classic case being Usher’s syndrome where the mutant mice do not have the same visual and auditory defects that patients face. There are others which have been even more difficult to model due to the multi-factorial nature of the condition and due to lack of discovery of a single causative gene such as age-related macular degeneration or Alzheimer’s syndrome. Thus a more relevant and accurate system will allow us to make better predictions on relevant therapeutic approaches. The discovery of human pluripotent stem cells in 1998 followed by the technological advances to reprogram somatic cells to pluripotent-stem cell-like cells in 2006 has completely revolutionized the way we can now think about modelling human development and disease. This now coupled with genome editing technologies such as TALENS and CRISPRs have now set us up to develop in vitro models both 2D as well as 3D organoids, which can more precisely reflect the disease in the patients. These combinatorial technologies are already providing us with better tools and therapeutics in drug discovery or gene therapy. This book summarizes both the technological advances in the field of generation of patient specific lines as well as various gene editing approaches followed by its applicability in various systems. The book will serve as a reference for the current state of the field as it: -Provides a comprehensive overview of the status of the field of patients derived induced pluripotent stem cells. -Describes the use of cardiac cells as a main featured component within the book. -Examines drug toxicity analysis as a working example throughout the book.

Author : Heather M. Wilkins,Levi Wood,Jill K. Morris
Publisher : Frontiers Media SA
Page : 183 pages
File Size : 46,9 Mb
Release : 2022-02-11
Category : Science
ISBN : 9782889743612

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Metabolism in Alzheimer’s Disease by Heather M. Wilkins,Levi Wood,Jill K. Morris Pdf

Author : Mayana Zatz,Oswaldo Keith Okamoto
Publisher : Springer
Page : 147 pages
File Size : 42,9 Mb
Release : 2015-08-11
Category : Science
ISBN : 9783319183145

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Stem Cells in Modeling Human Genetic Diseases by Mayana Zatz,Oswaldo Keith Okamoto Pdf

While most stem cell books focus on basic aspects and/or cell therapy, this book emphasizes the relevance of stem cells obtained from patients, the so-called “patients in a petri dish” as tools to investigate human genetic diseases for which there are no available effective treatment. Chapters embrace several examples of the use of iPS cell technology, a recent Nobel Prize-winning scientific breakthrough, to obtain patient-specific pluripotent cells from which many types of specialized cells involved in a particular disease can be generated, including psychiatric and neurodegenerative disorders, muscular dystrophies, laminopathies, among others. The text is a current and timely resource for postgraduate students, scientists and clinicians, interested in applications of this rapidly developing field of research in disease modeling, drug development, and emerging issues that it brings to regenerative medicine.

Author : Keiichi Fukuda
Publisher : Springer
Page : 99 pages
File Size : 43,6 Mb
Release : 2016-03-30
Category : Science
ISBN : 9784431559665

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Human iPS Cells in Disease Modelling by Keiichi Fukuda Pdf

Human iPS cells have a great potential to be cell sources for regenerative medicine because of the promise of infinite self-renewal and the capability to differentiate into multiple cell types. This book focuses on another great potential of human iPS cells, which is the establishment of human disease models using patient-specific iPS cells. Human iPS cells can be easily obtained from a patient’s somatic cells and provide the entire information on the patient’s genome. Accordingly, we can generate disease models for inheritable diseases in cell culture dishes using iPS cells. This is a quite new technique but holds tremendous potential for our increased understanding of pathogenesis, and will then be the basis for novel drug development industries. All the authors are leading researchers in this field and they have reported many kinds of patient-derived iPS cells. In this book, they introduce the aspects that could be recapitulated in terms of disease modelling as well as further innovative findings such as novel pathogenetic insights and novel therapies.

Author : Minoru Tomizawa
Publisher : BoD – Books on Demand
Page : 534 pages
File Size : 52,5 Mb
Release : 2016-07-20
Category : Science
ISBN : 9789535124719

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Pluripotent Stem Cells by Minoru Tomizawa Pdf

Pluripotent stem cells have distinct characteristics: self-renewal and the potential to differentiate into various somatic cells. In recent years, substantial advances have been made from basic science to clinical applications. The vast amount knowledge available makes obtaining concise yet sufficient information difficult, hence the purpose of this book. In this book, embryonic stem cells, induced pluripotent stem cells, and mesenchymal stem cells are discussed. The book is divided into five sections: pluripotency, culture methods, toxicology, disease models, and regenerative medicine. The topics covered range from new concepts to current technologies. Readers are expected to gain useful information from expert contributors.

Author : Alexander Birbrair
Publisher : Elsevier
Page : 324 pages
File Size : 52,5 Mb
Release : 2021-11-16
Category : Science
ISBN : 9780323857659

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Current Progress in IPSC Disease Modeling by Alexander Birbrair Pdf

Current Progress in iPSC Disease Modeling, Volume Fourteen in the Advances in Stem Cell Biology series, is a timely and expansive collection of information and new discoveries in the field. This new volume addresses advances in research on how induced pluripotent stem cells are used for the creation of new tissues and organs. The creation of iPSC technology allowed the development of disease-specific human pluripotent stem cells. These cells allow researchers to study questions once impossible for some human diseases. This volume addresses iPSCs for vascular tissue engineering, bioprinting, derived lung organoids for pulmonary disorders, skeletal muscle engineering, human kidney organoids, and more. It is written for researchers and scientists in stem cell therapy, cell biology, regenerative medicine and organ transplantation, and is contributed by world-renowned authors in the field. Provides an overview of the fast-moving field of stem cell biology and function, regenerative medicine and therapeutics Covers advances in research on how induced pluripotent stem cells are used to create new tissues/organs Contributed by world-renowned experts in the field

Author : ALEXANDER BIRBRAIR
Publisher : Academic Press
Page : 320 pages
File Size : 52,6 Mb
Release : 2020-07-30
Category : Science
ISBN : 9780128222270

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Recent Advances in iPSC Disease Modeling, Volume 1 by ALEXANDER BIRBRAIR Pdf

Recent Advances in iPSC Disease Modeling, Volume 1 addresses how induced pluripotent stem cells can be used to model various diseases. Somatic cells are reprogrammed into induced pluripotent stem cells by the expression of specific transcription factors. These cells are transforming biomedical research in the last 15 years. This volume teaches readers about current advances in the field. This book describes the use of induced pluripotent stem cells to model several diseases in vitro, enabling us to study the cellular and molecular mechanisms involved in different pathologies. Further insights into these mechanisms will have important implications for our understanding of disease appearance, development, and progression. In recent years, remarkable progress has been made in the obtention of induced pluripotent stem cells and their differentiation into several cell types, tissues, and organs using state-of-art techniques. These advantages facilitated identification of key targets and definition of the molecular basis of several disorders. The volume is written for researchers and scientists in stem cell therapy, cell biology, regenerative medicine and organ transplantation; and is contributed by world-renowned authors in the field. Provides overview of the fast-moving field of induced pluripotent stem cell technology, regenerative medicine, and therapeutics Covers the following diseases: severe congenital neutropenia, sickle cell and Diamond-Blackfan anemias, muscular dystrophies, Bernard-Soulier syndrome, familial hypercholesterolemia type II A, Werner syndrome, lysosomal storage diseases, and more Contains description of cutting-edge research on the development of disease-specific human pluripotent stem cells. These cells allow us to study cellular and molecular processes involved in several human diseases