Hydrogen Exchange Mass Spectrometry Of Proteins

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Hydrogen Exchange Mass Spectrometry of Proteins

David D. Weis
Hydrogen Exchange Mass Spectrometry of Proteins Book PDF

Author : David D. Weis
Publisher : John Wiley & Sons
Page : 376 pages
File Size : 48,8 Mb
Release : 2016-01-11
Category : Science
ISBN : 9781118703694

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Hydrogen Exchange Mass Spectrometry of Proteins by David D. Weis Pdf

Hydrogen exchange mass spectrometry is widely recognized for its ability to probe the structure and dynamics of proteins. The application of this technique is becoming widespread due to its versatility for providing structural information about challenging biological macromolecules such as antibodies, flexible proteins and glycoproteins. Although the technique has been around for 25 years, this is the first definitive book devoted entirely to the topic. Hydrogen Exchange Mass Spectrometry of Proteins: Fundamentals, Methods and Applications brings into one comprehensive volume the theory, instrumentation and applications of Hydrogen Exchange Mass Spectrometry (HX-MS) - a technique relevant to bioanalytical chemistry, protein science and pharmaceuticals. The book provides a solid foundation in the basics of the technique and data interpretation to inform readers of current research in the method, and provides illustrative examples of its use in bio- and pharmaceutical chemistry and biophysics In-depth chapters on the fundamental theory of hydrogen exchange, and tutorial chapters on measurement and data analysis provide the essential background for those ready to adopt HX-MS. Expert users may advance their current understanding through chapters on methods including membrane protein analysis, alternative proteases, millisecond hydrogen exchange, top-down mass spectrometry, histidine exchange and method validation. All readers can explore the diversity of HX-MS applications in areas such as ligand binding, membrane proteins, drug discovery, therapeutic protein formulation, biocomparability, and intrinsically disordered proteins.

Hydrogen Exchange Mass Spectrometry of Proteins

David D. Weis
Hydrogen Exchange Mass Spectrometry of Proteins Book PDF

Author : David D. Weis
Publisher : Wiley
Page : 376 pages
File Size : 44,9 Mb
Release : 2016-02-29
Category : Science
ISBN : 1118703715

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Hydrogen Exchange Mass Spectrometry of Proteins by David D. Weis Pdf

Hydrogen exchange mass spectrometry is widely recognized for its ability to probe the structure and dynamics of proteins. The application of this technique is becoming widespread due to its versatility for providing structural information about challenging biological macromolecules such as antibodies, flexible proteins and glycoproteins. Although the technique has been around for 25 years, this is the first definitive book devoted entirely to the topic. Hydrogen Exchange Mass Spectrometry of Proteins: Fundamentals, Methods and Applications brings into one comprehensive volume the theory, instrumentation and applications of Hydrogen Exchange Mass Spectrometry (HX-MS) - a technique relevant to bioanalytical chemistry, protein science and pharmaceuticals. The book provides a solid foundation in the basics of the technique and data interpretation to inform readers of current research in the method, and provides illustrative examples of its use in bio- and pharmaceutical chemistry and biophysics In-depth chapters on the fundamental theory of hydrogen exchange, and tutorial chapters on measurement and data analysis provide the essential background for those ready to adopt HX-MS. Expert users may advance their current understanding through chapters on methods including membrane protein analysis, alternative proteases, millisecond hydrogen exchange, top-down mass spectrometry, histidine exchange and method validation. All readers can explore the diversity of HX-MS applications in areas such as ligand binding, membrane proteins, drug discovery, therapeutic protein formulation, biocomparability, and intrinsically disordered proteins.

Mass Spectrometry of Protein Interactions

Kevin Downard
Mass Spectrometry of Protein Interactions Book PDF

Author : Kevin Downard
Publisher : John Wiley & Sons
Page : 153 pages
File Size : 55,6 Mb
Release : 2007-08-24
Category : Science
ISBN : 9780470146323

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Mass Spectrometry of Protein Interactions by Kevin Downard Pdf

The authoritative guide to analyzing protein interactions by mass spectrometry Mass spectrometry (MS) is playing an increasingly important role in the study of protein interactions. Mass Spectrometry of Protein Interactionspresents timely and definitive discussions of the diverse range of approaches for studying protein interactions by mass spectrometry with an extensive set of references to the primary literature. Each chapter is written by authors or teams of authors who are international authorities in their fields. This leading reference text: * Discusses the direct detection of protein interactions through electrospray ionization (ESI-MS); ion mobility analysis; and matrix-assisted laser desorption/ionization (MALDI-MS) * Covers the indirect analysis of protein interactions through hydrogen-deuterium exchange (HX-MS); limited proteolysis; cross-linking; and radial probe (RP-MS) * Guides researchers in the use of mass spectrometry in structural biology, biochemistry, and protein science to map and define the huge number and diversity of protein interactions * Reviews the latest discoveries and applications and addresses new and ongoing challenges This is a comprehensive reference for researchers in academia and industry engaged in studies of protein interactions and an excellent text for graduate and postgraduate students.

Protein Mass Spectrometry

Julian Whitelegge
Protein Mass Spectrometry Book PDF

Author : Julian Whitelegge
Publisher : Elsevier
Page : 552 pages
File Size : 51,5 Mb
Release : 2008-10-09
Category : Science
ISBN : 0080932037

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Protein Mass Spectrometry by Julian Whitelegge Pdf

This book is designed to be a central text for young graduate students interested in mass spectrometry as it relates to the study of protein structure and function as well as proteomics. It is a definite must-have work for: - libraries at academic institutions with Master and Graduate programs in biochemistry, molecular biology, structural biology and proteomics - individual laboratories with interests covering these areas - libraries and individual laboratories in the pharmaceutical and biotechnology industries. *Serves as an essential reference to those working in the field *Incorporates the contributions of prominent experts *Features comprehensive coverage and a logical structure

Application and Development of Amide Hydrogen Exchange and MALDI Mass Spectrometry to Protein Folding and Stability

Yan Tong
Application and Development of Amide Hydrogen Exchange and MALDI Mass Spectrometry to Protein Folding and Stability Book PDF

Author : Yan Tong
Publisher : Unknown
Page : 159 pages
File Size : 45,8 Mb
Release : 2006
Category : Matrix-assisted laser desorption-ionization
ISBN : 0549022791

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Application and Development of Amide Hydrogen Exchange and MALDI Mass Spectrometry to Protein Folding and Stability by Yan Tong Pdf

Many methods have been developed to provide thermodynamic measurements of protein stability. These measurements are of great importance as they are widely used in biophysical studies of protein folding and function. Recently, an amide hydrogen exchange- and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry-based method, termed SUPREX (stability of unpurified proteins from rates of H/D exchange), was reported to make such thermodynamic measurements. The SUPREX technique removes some important experimental limitations associated with conventional spectroscopic and calorimetric methods for making similar thermodynamic measurements. This dissertation is focused on the application of SUPREX technique to several new protein systems.

Mass Spectrometry Analysis for Protein-Protein Interactions and Dynamics

M. Chance
Mass Spectrometry Analysis for Protein Protein Interactions and Dynamics Book PDF

Author : M. Chance
Publisher : Wiley
Page : 0 pages
File Size : 44,7 Mb
Release : 2008-09-22
Category : Science
ISBN : 0470258861

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Mass Spectrometry Analysis for Protein-Protein Interactions and Dynamics by M. Chance Pdf

Presents a wide variety of mass spectrometry methods used to explore structural mechanisms, protein dynamics and interactions between proteins. Preliminary chapters cover mass spectrometry methods for examining proteins and are then followed by chapters devoted to presenting very practical, how-to methods in a detailed way. Includes footprinting and plistex specifically, setting this book apart from the competition.

Biological Mass Spectrometry

A.L. Burlingame
Biological Mass Spectrometry Book PDF

Author : A.L. Burlingame
Publisher : Gulf Professional Publishing
Page : 520 pages
File Size : 55,6 Mb
Release : 2005-11-28
Category : Science
ISBN : 0121828077

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Biological Mass Spectrometry by A.L. Burlingame Pdf

Describes and integrates the techniques of many advances in both chromatographic and mass spectrometric technologies. This book also covers various biophysical applications, such as H/D exchange for study of conformations, protein-protein and protein-metal and ligand interactions. It also describes atto-to-zepto-mole quantitation of 14C and 3H.

Applied Biophysics for Drug Discovery

Donald Huddler,Edward R. Zartler
Applied Biophysics for Drug Discovery Book PDF

Author : Donald Huddler,Edward R. Zartler
Publisher : John Wiley & Sons
Page : 148 pages
File Size : 45,7 Mb
Release : 2017-10-02
Category : Science
ISBN : 9781119099482

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Applied Biophysics for Drug Discovery by Donald Huddler,Edward R. Zartler Pdf

Applied Biophysics for Drug Discovery is a guide to new techniques and approaches to identifying and characterizing small molecules in early drug discovery. Biophysical methods are reasserting their utility in drug discovery and through a combination of the rise of fragment-based drug discovery and an increased focus on more nuanced characterisation of small molecule binding, these methods are playing an increasing role in discovery campaigns. This text emphasizes practical considerations for selecting and deploying core biophysical method, including but not limited to ITC, SPR, and both ligand-detected and protein-detected NMR. Topics covered include: • Design considerations in biophysical-based lead screening • Thermodynamic characterization of protein-compound interactions • Characterizing targets and screening reagents with HDX-MS • Microscale thermophoresis methods (MST) • Screening with Weak Affinity Chromatography • Methods to assess compound residence time • 1D-NMR methods for hit identification • Protein-based NMR methods for SAR development • Industry case studies integrating multiple biophysical methods This text is ideal for academic investigators and industry scientists planning hit characterization campaigns or designing and optimizing screening strategies.

Hydrogen Exchange Mass Spectrometry of Proteins

David D. Weis
Hydrogen Exchange Mass Spectrometry of Proteins Book PDF

Author : David D. Weis
Publisher : John Wiley & Sons
Page : 422 pages
File Size : 52,7 Mb
Release : 2016-03-21
Category : Science
ISBN : 9781118616499

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Hydrogen Exchange Mass Spectrometry of Proteins by David D. Weis Pdf

Hydrogen exchange mass spectrometry is widely recognized for its ability to probe the structure and dynamics of proteins. The application of this technique is becoming widespread due to its versatility for providing structural information about challenging biological macromolecules such as antibodies, flexible proteins and glycoproteins. Although the technique has been around for 25 years, this is the first definitive book devoted entirely to the topic. Hydrogen Exchange Mass Spectrometry of Proteins: Fundamentals, Methods and Applications brings into one comprehensive volume the theory, instrumentation and applications of Hydrogen Exchange Mass Spectrometry (HX-MS) - a technique relevant to bioanalytical chemistry, protein science and pharmaceuticals. The book provides a solid foundation in the basics of the technique and data interpretation to inform readers of current research in the method, and provides illustrative examples of its use in bio- and pharmaceutical chemistry and biophysics In-depth chapters on the fundamental theory of hydrogen exchange, and tutorial chapters on measurement and data analysis provide the essential background for those ready to adopt HX-MS. Expert users may advance their current understanding through chapters on methods including membrane protein analysis, alternative proteases, millisecond hydrogen exchange, top-down mass spectrometry, histidine exchange and method validation. All readers can explore the diversity of HX-MS applications in areas such as ligand binding, membrane proteins, drug discovery, therapeutic protein formulation, biocomparability, and intrinsically disordered proteins.

Mass Spectrometry in Biophysics

Igor A. Kaltashov,Stephen J. Eyles
Mass Spectrometry in Biophysics Book PDF

Author : Igor A. Kaltashov,Stephen J. Eyles
Publisher : John Wiley & Sons
Page : 320 pages
File Size : 50,5 Mb
Release : 2005-05-06
Category : Science
ISBN : 9780471705161

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Mass Spectrometry in Biophysics by Igor A. Kaltashov,Stephen J. Eyles Pdf

The first systematic summary of biophysical mass spectrometrytechniques Recent advances in mass spectrometry (MS) have pushed the frontiersof analytical chemistry into the biophysical laboratory. As aresult, the biophysical community's acceptance of MS-based methods,used to study protein higher-order structure and dynamics, hasaccelerated the expansion of biophysical MS. Despite this growing trend, until now no single text has presentedthe full array of MS-based experimental techniques and strategiesfor biophysics. Mass Spectrometry in Biophysics expertly closesthis gap in the literature. Covering the theoretical background and technical aspects of eachmethod, this much-needed reference offers an unparalleled overviewof the current state of biophysical MS. Mass Spectrometry inBiophysics begins with a helpful discussion of general biophysicalconcepts and MS-related techniques. Subsequent chaptersaddress: * Modern spectrometric hardware * High-order structure and dynamics as probed by various MS-basedmethods * Techniques used to study structure and behavior of non-nativeprotein states that become populated under denaturingconditions * Kinetic aspects of protein folding and enzyme catalysis * MS-based methods used to extract quantitative information onprotein-ligand interactions * Relation of MS-based techniques to other experimental tools * Biomolecular properties in the gas phase Fully referenced and containing a helpful appendix on the physicsof electrospray mass spectrometry, Mass Spectrometry in Biophysicsalso offers a compelling look at the current challenges facingbiomolecular MS and the potential applications that will likelyshape its future.

Biophysical Characterization of Proteins in Developing Biopharmaceuticals

Damian J. Houde,Steven A. Berkowitz
Biophysical Characterization of Proteins in Developing Biopharmaceuticals Book PDF

Author : Damian J. Houde,Steven A. Berkowitz
Publisher : Elsevier
Page : 586 pages
File Size : 40,5 Mb
Release : 2019-11-13
Category : Medical
ISBN : 9780444641748

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Biophysical Characterization of Proteins in Developing Biopharmaceuticals by Damian J. Houde,Steven A. Berkowitz Pdf

Biophysical Characterization of Proteins in Developing Biopharmaceuticals, Second Edition, presents the latest on the analysis and characterization of the higher-order structure (HOS) or conformation of protein based drugs. Starting from the very basics of protein structure, this book explains the best way to achieve this goal using key methods commonly employed in the biopharmaceutical industry. This book will help today’s industrial scientists plan a career in this industry and successfully implement these biophysical methodologies. This updated edition has been fully revised, with new chapters focusing on the use of chromatography and electrophoresis and the biophysical characterization of very large biopharmaceuticals. In addition, best practices of applying statistical analysis to biophysical characterization data is included, along with practical issues associated with the concept of a biopharmaceutical’s developability and the technical decision-making process needed when dealing with biophysical characterization data. Presents basic protein characterization methods and tools applicable to (bio)pharmaceutical research and development Highlights the capabilities and limitations of each technique Discusses the underlining science of each tool Empowers industrial biophysical chemists by providing a roadmap for applying biophysical tools Outlines the needs for new characterization and analytical tools in the biopharmaceutical industry

Protein Complex Assembly

Joseph A. Marsh
Protein Complex Assembly Book PDF

Author : Joseph A. Marsh
Publisher : Humana
Page : 506 pages
File Size : 46,7 Mb
Release : 2019-04-15
Category : Science
ISBN : 1493992775

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Protein Complex Assembly by Joseph A. Marsh Pdf

This volume details the importance of multiple experimental techniques and computational methods needed to obtain the comprehensive picture of protein complex structure, dynamics and assembly afforded by the emerging field of integrative structural biology. Chapters guide readers through the broad spectrum of approaches required for a complete representation of protein complexes, including expression and purification, experimental characterization of structure and assembly, and computational methods for identifying protein complexes and modelling their assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Protein Complex Assembly: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Characterization of Protein Therapeutics using Mass Spectrometry

Guodong Chen
Characterization of Protein Therapeutics using Mass Spectrometry Book PDF

Author : Guodong Chen
Publisher : Springer Science & Business Media
Page : 404 pages
File Size : 49,5 Mb
Release : 2014-07-08
Category : Science
ISBN : 9781441978622

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Characterization of Protein Therapeutics using Mass Spectrometry by Guodong Chen Pdf

This book highlights current approaches and future trends in the use of mass spectrometry to characterize protein therapies. As one of the most frequently utilized analytical techniques in pharmaceutical research and development, mass spectrometry has been widely used in the characterization of protein therapeutics due to its analytical sensitivity, selectivity, and specificity. This book begins with an overview of mass spectrometry techniques as related to the analysis of protein therapeutics, structural identification strategies, quantitative approaches, followed by studies involving characterization of process related protein drug impurities/degradants, metabolites, higher order structures of protein therapeutics. Both general practitioners in pharmaceutical research and specialists in analytical sciences will benefit from this book that details step-by-step approaches and new strategies to solve challenging problems related to protein therapeutics research and development.

Mass Spectrometry-Based Chemical Proteomics

W. Andy Tao,Ying Zhang
Mass Spectrometry Based Chemical Proteomics Book PDF

Author : W. Andy Tao,Ying Zhang
Publisher : John Wiley & Sons
Page : 448 pages
File Size : 51,5 Mb
Release : 2019-07-10
Category : Science
ISBN : 9781118970218

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Mass Spectrometry-Based Chemical Proteomics by W. Andy Tao,Ying Zhang Pdf

PROVIDES STRATEGIES AND CONCEPTS FOR UNDERSTANDING CHEMICAL PROTEOMICS, AND ANALYZING PROTEIN FUNCTIONS, MODIFICATIONS, AND INTERACTIONS—EMPHASIZING MASS SPECTROMETRY THROUGHOUT Covering mass spectrometry for chemical proteomics, this book helps readers understand analytical strategies behind protein functions, their modifications and interactions, and applications in drug discovery. It provides a basic overview and presents concepts in chemical proteomics through three angles: Strategies, Technical Advances, and Applications. Chapters cover those many technical advances and applications in drug discovery, from target identification to validation and potential treatments. The first section of Mass Spectrometry-Based Chemical Proteomics starts by reviewing basic methods and recent advances in mass spectrometry for proteomics, including shotgun proteomics, quantitative proteomics, and data analyses. The next section covers a variety of techniques and strategies coupling chemical probes to MS-based proteomics to provide functional insights into the proteome. In the last section, it focuses on using chemical strategies to study protein post-translational modifications and high-order structures. Summarizes chemical proteomics, up-to-date concepts, analysis, and target validation Covers fundamentals and strategies, including the profiling of enzyme activities and protein-drug interactions Explains technical advances in the field and describes on shotgun proteomics, quantitative proteomics, and corresponding methods of software and database usage for proteomics Includes a wide variety of applications in drug discovery, from kinase inhibitors and intracellular drug targets to the chemoproteomics analysis of natural products Addresses an important tool in small molecule drug discovery, appealing to both academia and the pharmaceutical industry Mass Spectrometry-Based Chemical Proteomics is an excellent source of information for readers in both academia and industry in a variety of fields, including pharmaceutical sciences, drug discovery, molecular biology, bioinformatics, and analytical sciences.

Mass Spectrometry in Structural Biology and Biophysics

Igor A. Kaltashov,Stephen J. Eyles
Mass Spectrometry in Structural Biology and Biophysics Book PDF

Author : Igor A. Kaltashov,Stephen J. Eyles
Publisher : John Wiley & Sons
Page : 312 pages
File Size : 44,5 Mb
Release : 2012-03-02
Category : Science
ISBN : 9781118232118

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Mass Spectrometry in Structural Biology and Biophysics by Igor A. Kaltashov,Stephen J. Eyles Pdf

The definitive guide to mass spectrometry techniques in biology and biophysics The use of mass spectrometry (MS) to study the architecture and dynamics of proteins is increasingly common within the biophysical community, and Mass Spectrometry in Structural Biology and Biophysics: Architecture, Dynamics, and Interaction of Biomolecules, Second Edition provides readers with detailed, systematic coverage of the current state of the art. Offering an unrivalled overview of modern MS-based armamentarium that can be used to solve the most challenging problems in biophysics, structural biology, and biopharmaceuticals, the book is a practical guide to understanding the role of MS techniques in biophysical research. Designed to meet the needs of both academic and industrial researchers, it makes mass spectrometry accessible to professionals in a range of fields, including biopharmaceuticals. This new edition has been significantly expanded and updated to include the most recent experimental methodologies and techniques, MS applications in biophysics and structural biology, methods for studying higher order structure and dynamics of proteins, an examination of other biopolymers and synthetic polymers, such as nucleic acids and oligosaccharides, and much more. Featuring high-quality illustrations that illuminate the concepts described in the text, as well as extensive references that enable the reader to pursue further study, Mass Spectrometry in Structural Biology and Biophysics is an indispensable resource for researchers and graduate students working in biophysics, structural biology, protein chemistry, and related fields.