Immobilization Of Enzymes And Cells

Author : Jose M. Guisan
Publisher : Humana
Page : 0 pages
File Size : 42,7 Mb
Release : 2016-08-27
Category : Science
ISBN : 1493959883

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Immobilization of Enzymes and Cells by Jose M. Guisan Pdf

For most of industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used for very long periods of time. From this point of view, immobilization, simplicity and stabilization have to be strongly related concepts. The third edition of Immobilization of Enzymes and Cells expands upon and updates the previous editions with current, detailed protocols for immobilization. With new chapters on protocols for immobilization of enzymes and cells which may be useful to greatly improve the functional properties of enzymes and cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Immobilization of Enzymes and Cells, Third Edition demonstrates simple and efficient protocols for the preparation, characterization, and utilization of immobilized enzymes and cells.

Author : Jose M. Guisan,Juan M. Bolivar,Fernando López-Gallego,Javier Rocha-Martín
Publisher : Humana
Page : 495 pages
File Size : 44,7 Mb
Release : 2021-01-29
Category : Science
ISBN : 1071602179

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Immobilization of Enzymes and Cells by Jose M. Guisan,Juan M. Bolivar,Fernando López-Gallego,Javier Rocha-Martín Pdf

This fourth edition volume expands on the previous editions with new insights on important aspects to take into accounting when immobilizing enzymes and cells, illustrating outstanding examples that support those aspects, and exploring ways to fabricate and characterize heterogeneous biocatalysts including both immobilized enzymes and cells. The transformation of soluble and usually instable enzymes into heterogeneous and highly stable biocatalysts is strongly emphasized. The chapters in this book cover topics such as the importance of enzyme orientation on the support surface; application and characterization of immobilized enzymes; different functionalization chemistries for the modulation of the immobilized enzyme properties; co-immobilization of multi-enzyme systems; new analytical techniques for the characterization of heterogeneous biocatalysts; protocols for cell entrapment in alginate; preparation and characterization of biofilms; and cell encapsulation technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Immobilization of Enzymes and Cells: Methods and Protocols, Fourth Edition is a valuable resource for researchers interested in expanding their knowledge of this developing field.

Author : José M. Guisán
Publisher : Springer Science & Business Media
Page : 447 pages
File Size : 48,8 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781597450539

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Immobilization of Enzymes and Cells by José M. Guisán Pdf

Enzymes and whole cells are able to catalyze the most complex chemical processes under the most benign experimental and environmental conditions. In this way, enzymes and cells could be excellent catalysts for a much more sustainable chemical industry. However, enzymes and cells also have some limitations for nonbiological applications: fine chemistry, food chemistry, analysis, therapeutics, and so on. Enzymes and cells may be unstable, difficult to handle under nonconventional conditions, poorly selective toward synthetic substrates, and so forth. From this point of view, the transformation—from the laboratory to industry—of chemical processes catalyzed by enzymes and cells may be one of the most complex and exciting goals in biotechnology. For many industrial applications, enzymes and cells have to be immobilized, via very simple and cost-effective protocols, in order to be re-used over very long periods of time. From this point of view, immobilization, simplicity, and stabilization have to be strongly related concepts. Over the last 30 years, a number of protocols for the immobilization of cells and enzymes have been reported in scientific literature. However, only very few protocols are simple and useful enough to greatly improve the functional properties of enzymes and cells, activity, stability, selectivity, and related properties.

Author : Colin R. Phillips,Yiu Cheong Poon
Publisher : Springer Science & Business Media
Page : 171 pages
File Size : 40,6 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642732492

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Immobilization of Cells by Colin R. Phillips,Yiu Cheong Poon Pdf

Growth of immobilized cells can be viewed as an alternative to growth of free cells in many instances. In others, immobilization confers a precision of control over the process not possible in free growth. Immobilization of cells can sometimes be considered to be a lower cost alternative to immobilization of enzymes. In this volume, immobilization procedures based on mechanical means and bonding of various types are examined, with detailed application examples. These applications include microorganisms, plant and animal cells, sub-cellular organelles and multiple enzyme systems. Particular attention is devoted to enzyme properties in immobilized cells and the properties of the carrier. The volume should provide the reader with a comprehensive overview of the subject, together with copious references. As well as serving as a research monograph, it could be used to provide reference material for a graduate course. Special thanks are due Mrs. JENNIFER KERBY for her dedicated work in the preparation of the manuscript, and IT-CHIN HSIEH for bibliographical assistance. COLIN R. PHILLIPS Toronto, July 1988 YIU C. POON v Table of Contents 1 Introduction. 1 References . 9 2 Methods of Cell Immobilization 11 2.1 Mechanical Immobilization . 11 2.1.1 Mycelial Pellet and Mat 11 2.1.2 Encapsulation .. 48 2.1.3 Dialysis Culture. . . 49 2.1.4 Entrapment. .... 50 2.2 Covalent Attachment 61 2.3 Ionic Attachment 62 2.3.1 Flocculation 62 2.3.2 Adsorption . 64 References . 66 3 Special Problems and Extended Applications . 75 3.1 Special Problems and Techniques .

Author : Winfried Hartmeier
Publisher : Springer Science & Business Media
Page : 217 pages
File Size : 55,9 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642733642

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Immobilized Biocatalysts by Winfried Hartmeier Pdf

Biotechnological processes often require the use of immobilized biocatalysts. This translation of the most successful German edition introduces the theoretical background of this rapidly growing field followed by a detailed description of the various techniques of immobilizing, characterizing, and using biocatalysts. The comprehensive discussion of possible applications in industrial processes and in basic research results in a practical guide for everyone involved in these techniques. Furthermore, some selected experiments are added in order to facilitate the understanding of the theoretical reports.

Author : Jose M. Guisan,Juan M. Bolivar,Fernando López-Gallego,Javier Rocha-Martín
Publisher : Humana
Page : 0 pages
File Size : 52,5 Mb
Release : 2020-01-15
Category : Science
ISBN : 1071602144

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Immobilization of Enzymes and Cells by Jose M. Guisan,Juan M. Bolivar,Fernando López-Gallego,Javier Rocha-Martín Pdf

This fourth edition volume expands on the previous editions with new insights on important aspects to take into accounting when immobilizing enzymes and cells, illustrating outstanding examples that support those aspects, and exploring ways to fabricate and characterize heterogeneous biocatalysts including both immobilized enzymes and cells. The transformation of soluble and usually instable enzymes into heterogeneous and highly stable biocatalysts is strongly emphasized. The chapters in this book cover topics such as the importance of enzyme orientation on the support surface; application and characterization of immobilized enzymes; different functionalization chemistries for the modulation of the immobilized enzyme properties; co-immobilization of multi-enzyme systems; new analytical techniques for the characterization of heterogeneous biocatalysts; protocols for cell entrapment in alginate; preparation and characterization of biofilms; and cell encapsulation technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and authoritative, Immobilization of Enzymes and Cells: Methods and Protocols, Fourth Edition is a valuable resource for researchers interested in expanding their knowledge of this developing field.

Author : Rowan Austin
Publisher : Scientific e-Resources
Page : 348 pages
File Size : 53,5 Mb
Release : 2018-12-19
Category : Electronic
ISBN : 9781839473463

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Enzyme Immobilization by Rowan Austin Pdf

An enzyme is a protein, or protein complex, that catalyses a chemical reaction. Like any catalyst, enzymes work by lowering the activation energy of a reaction, thus allowing the reaction to proceed to its steady state or completion much faster than it otherwise would; the enzyme remains unaltered by the completed reaction and can therefore continue catalysis. An immobilized enzyme is an enzyme attached to an inert, insoluble material-such as calcium alginate. This can provide increased resistance to changes in conditions such as pH or temperature. It also lets enzymes be held in place throughout the reaction, following which they are easily separated from the products and may be used again-a far more efficient process and so is widely used in industry for enzyme catalysed reactions. An alternative to enzyme immobilization is whole cell immobilization. Carrier matrices for enzyme immobilisation by adsorption and covalent binding must be chosen with care. The manufacture of high-valued products on a small scale may allow the use of relatively expensive supports and immobilisation techniques whereas these would not be economical in the large-scale production of low added-value materials. A substantial saving in costs occurs where the carrier may be regenerated after the useful lifetime of the immobilised enzyme. This book is simple protocols for the immobilization of enzymes and cells that could be useful for application at industrial scale, novel protocols for immobilization in the future, and new chemical reactors able to overcome the limitations of a number of immobilized derivatives.

Author : Allen I. Laskin
Publisher : Butterworth-Heinemann
Page : 344 pages
File Size : 41,8 Mb
Release : 1985
Category : Science
ISBN : UOM:39015009827208

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Enzymes and Immobilized Cells in Biotechnology by Allen I. Laskin Pdf

Author : Klaus Mosbach
Publisher : Unknown
Page : 718 pages
File Size : 44,9 Mb
Release : 1987
Category : Cells
ISBN : WISC:89031249725

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Immobilized Enzymes and Cells by Klaus Mosbach Pdf

Author : A Rosevear,J.F Kennedy,Joaquim M.S. Cabral
Publisher : CRC Press
Page : 248 pages
File Size : 49,6 Mb
Release : 1987-01-01
Category : Science
ISBN : 085274515X

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Immobilised Enzymes and Cells by A Rosevear,J.F Kennedy,Joaquim M.S. Cabral Pdf

During the past twenty years the immobilisation of enzymes and cells has developed into a major topic of theoretical and practical importance. This technology unites the disciplines of chemistry, biochemistry and cell biology on one hand with biochemical and process engineering on the other. As well as having an importance in its own right, its use has already made an impact on downstream processing of biochemicals. In this handbook the authors, who are experts in their field, bring together for the first time in a single volume information on the techniques of immobilisation and the uses of the immobilised enzymes and cells. They provide an overview of the subject, as well as practical guidance on the choice and relative merits of many techniques. Carefully selected examples taken from the literature illustrate the general principles. The balance between theory and practice provides a text which will introduce the topic to research students and also provide a source of practical details on efficient immobilisation methods for workers who's only interest is in exploiting the methods in their particular field. This is primarily a laboratory book, and as such it should be found dog-eared and stained on a laboratory bench rather than gathering dust on a library shelf. Key references are given to enable the reader to explore the literature in depth. Information is also supplied on journals, enzyme and equipment suppliers.

Author : Wen-Yong Lou,Jun Ge,Changzhu Wu,Jesús Fernández Lucas
Publisher : Frontiers Media SA
Page : 492 pages
File Size : 46,8 Mb
Release : 2021-04-21
Category : Science
ISBN : 9782889667093

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Enzyme or Whole Cell Immobilization for Efficient Biocatalysis: Focusing on Novel Supporting Platforms and Immobilization Techniques by Wen-Yong Lou,Jun Ge,Changzhu Wu,Jesús Fernández Lucas Pdf

Author : Gordon Bickerstaff
Publisher : Humana Press
Page : 367 pages
File Size : 53,6 Mb
Release : 1996-12-01
Category : Science
ISBN : 0896033864

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Immobilization of Enzymes and Cells by Gordon Bickerstaff Pdf

Immobihzatron of enzymes, cells, and organelles has expanded greatly in the past 30 years as the advantages of immobilization have been evaluated and utilized in analyttcal, biotransformation, and medical applications. A c- sequence of this explosion of technology IS that there is now a bewildering array of permutations for the immobilization of biological material. The p- pose of Immobilization of Enzymes and Cells is to provrde a basic reference tool for all academic and industrial research workers seeking to start or expand the use of mnnobilization techniques in their work. The book does not aim to provide comprehensive coverage of the vast range of methods available, but will serve as a launch pad for potential users of immobilization techniques. One reason for the vast expanse of mmrobilization technology lies m the subject material to be immobilized. Biological catalysts (enzymes, organelles, and cells) have a high degree of individual variability, and although many tmmobilization techniques have wide applicability, tt is imposstble for one or even a few methods to cater to the great diversity of requirements inherent in biological material. This is especially so when the atm is to produce an op- mum system m which the immobihzed biocatalyst will function at high levels of efficrency, stability, and so on.

Author : Ravishankar Rai V
Publisher : John Wiley & Sons
Page : 750 pages
File Size : 48,8 Mb
Release : 2015-12-21
Category : Technology & Engineering
ISBN : 9781118864555

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Advances in Food Biotechnology by Ravishankar Rai V Pdf

The application of biotechnology in the food sciences has led to an increase in food production and enhanced the quality and safety of food. Food biotechnology is a dynamic field and the continual progress and advances have not only dealt effectively with issues related to food security but also augmented the nutritional and health aspects of food. Advances in Food Biotechnology provides an overview of the latest development in food biotechnology as it relates to safety, quality and security. The seven sections of the book are multidisciplinary and cover the following topics: GMOs and food security issues Applications of enzymes in food processing Fermentation technology Functional food and nutraceuticals Valorization of food waste Detection and control of foodborne pathogens Emerging techniques in food processing Bringing together experts drawn from around the world, the book is a comprehensive reference in the most progressive field of food science and will be of interest to professionals, scientists and academics in the food and biotech industries. The book will be highly resourceful to governmental research and regulatory agencies and those who are studying and teaching food biotechnology.

Author : Ichiro Chibata,Lemuel B. Wingard
Publisher : Elsevier
Page : 372 pages
File Size : 41,8 Mb
Release : 2014-06-28
Category : Science
ISBN : 9781483215860

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Immobilized Microbial Cells by Ichiro Chibata,Lemuel B. Wingard Pdf

Immobilized Microbial Cells, Volume 4 provides an overview of the methods of immobilization, applications, and ways of utilizing immobilized microbial cells and subcellular organelles and chloroplasts as biocatalysts. This volume is comprised of seven chapters. It begins with the historical background of immobilized cell research. Subsequent chapters focus on the methods of immobilization and applications of immobilized microbial cells, living cells, and organelles. The last two chapters discuss gas production of immobilized cells for energy generation and the chemical engineering analysis of immobilized-cell systems. The book will be of great use to chemists and chemical engineers.

Author : Viktor Nedovic,Ronnie Willaert
Publisher : Springer Science & Business Media
Page : 542 pages
File Size : 44,5 Mb
Release : 2013-04-17
Category : Science
ISBN : 9789401716383

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Fundamentals of Cell Immobilisation Biotechnology by Viktor Nedovic,Ronnie Willaert Pdf

Cell Immobilisation Biotechnology Biotechnology is divided into two volumes. The first volume is dedicated to fundamental aspects of cell immobilisation while the second volume deals with the diverse applications of this technology. The first volume, Fundamentals of Cell Immobilisation Biotechnology, comprises 26 chapters arranged into four parts: Materials for cell immobilisation/encapsulation, Methods and technologies for cell immobilisation/encapsulation, Carrier characterisation and bioreactor design, and Physiology of immobilised cells: techniques and mathematical modelling.