Intracellular Delivery

Author : Aleš Prokop,Volkmar Weissig
Publisher : Springer
Page : 453 pages
File Size : 43,5 Mb
Release : 2016-10-31
Category : Medical
ISBN : 9783319435251

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Intracellular Delivery III by Aleš Prokop,Volkmar Weissig Pdf

A critical review is attempted to assess the status of nanomedicine entry onto the market. The emergence of new potential therapeutic entities such as DNA and RNA fragments requires that these new “drugs” will need to be delivered in a cell-and organelle-specific manner. Although efforts have been made over the last 50 years or so to develop such delivery technology, no effective and above all clinically approved protocol for cell-specific drug delivery in humans exists as yet. Various particles, macromolecules, liposomes and most recently “nanomaterials” have been said to “show promise” but none of these promises have so far been “reduced” to human clinical practice. The focus of this volume is on cancer indication since the majority of published research relates to this application; within that, we focus on solid tumors (solid malignancies). Our aim is critically to evaluate whether nanomaterials, both non-targeted and targeted to specific cells, could be of therapeutic benefit in clinical practice. The emphasis of this volume will be on pharmacokinetics (PK) and pharmacodynamics (PD) in animal and human studies. Apart from the case of exquisitely specific antibody-based drugs, the development of target-specific drug–carrier delivery systems has not yet been broadly successful at the clinical level. It can be argued that drugs generated using the conventional means of drug development (i.e., relying on facile biodistribution and activity after (preferably) oral administration) are not suitable for a target-specific delivery and would not benefit from such delivery even when a seemingly perfect delivery system is available. Therefore, successful development of site-selective drug delivery systems will need to include not only the development of suitable carriers, but also the development of drug entities that meet the required PK/PD profile.

Author : Aleš Prokop
Publisher : Springer Science & Business Media
Page : 871 pages
File Size : 51,9 Mb
Release : 2011-05-26
Category : Science
ISBN : 9789400712485

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Intracellular Delivery by Aleš Prokop Pdf

This book features a special subsection of Nanomedicine, an application of nanotechnology to achieve breakthroughs in healthcare. It exploits the improved and often novel physical, chemical and biological properties of materials only existent at the nanometer scale. As a consequence of small scale, nanosystems in most cases are efficiently uptaken by cells and appear to act at the intracellular level. Nanotechnology has the potential to improve diagnosis, treatment and follow-up of diseases, and includes targeted drug delivery and regenerative medicine; it creates new tools and methods that impact significantly upon existing conservative practices. This volume is a collection of authoritative reviews. In the introductory section we define the field (intracellular delivery). Then, the fundamental routes of nanodelivery devices, cellular uptake, types of delivery devices, particularly in terms of localized cellular delivery, both for small drug molecules, macromolecular drugs and genes; at the academic and applied levels, are covered. The following section is dedicated to enhancing delivery via special targeting motifs followed by the introduction of different types of intracellular nanodelivery devices (e.g. a brief description of their chemistry) and ways of producing these different devices. Finally, we put special emphasis on particular disease states and on other biomedical applications, whilst diagnostic and sensing issues are also included. Intracellular delivery / therapy is a highly topical which will stir great interest. Intracellular delivery enables much more efficient drug delivery since the impact (on different organelles and sites) is intracellular as the drug is not supplied externally within the blood stream. There is great potential for targeted delivery with improved localized delivery and efficacy.

Author : Padma V. Devarajan,Prajakta Dandekar,Anisha A. D'Souza
Publisher : Springer Nature
Page : 560 pages
File Size : 47,6 Mb
Release : 2019-11-09
Category : Medical
ISBN : 9783030291686

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Targeted Intracellular Drug Delivery by Receptor Mediated Endocytosis by Padma V. Devarajan,Prajakta Dandekar,Anisha A. D'Souza Pdf

This book elaborates on drug delivery targeting via intracellular delivery, specifically through the Receptor Mediated Endocytosis (RME) approach, due to the involvement of cellular receptors in various grave diseases. Targeted delivery relies on two basic approaches, passive and active targeting. While passive targeting approaches have shown great promise, the improved selectivity achieved with active targeting approaches has resulted in significantly higher efficacy. Interestingly there are numerous strategies for active targeting, many of which are already highlighted in , Targeted Drug Delivery: Concepts and Applications. Nevertheless an exciting and practical strategy for active targeting, which could enable high intracellular delivery, is through exploitation of RME. Cells in the body express receptors to enable various physiological and biochemical processes. As a result, many of these receptors are overexpressed in pathological conditions, or newer receptors expressed due to defective cellular functioning. RME is based on exploitation of such receptors to achieve intracellular delivery. While targeted delivery can have manifold applications, in this book we focus on two major and challenging therapeutic areas; i) Cancer and ii) Infectious Diseases. Targeted Intracellular Drug Delivery by Receptor Medicated Endocytosis discusses the major receptors that are useful for targeted delivery for these afflictions. A major section of this book is dedicated to details regarding their occurrence and location, the recognition domain of the receptor, structure activity relationship of substrate /ligand for selective binding, ligands explored, antagonists for ligand binding and relevance of these aspects for therapy of cancer and infectious diseases. These facets are elucidated with the help of specific examples from academic research and also emphasize commercial products, wherever relevant. In vitro cellular models relied on for assessing receptor mediated cellular targeting and in vivo models depicting clinical efficacy are focused on in a separate section. Finally, we briefly discuss the regulatory and toxicity issues that may be associated specifically with the RME approach of intracellular drug delivery.

Author : Aleš Prokop,Yasuhiko Iwasaki,Atsushi Harada
Publisher : Springer
Page : 479 pages
File Size : 48,9 Mb
Release : 2014-05-20
Category : Medical
ISBN : 9789401788960

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Intracellular Delivery II by Aleš Prokop,Yasuhiko Iwasaki,Atsushi Harada Pdf

This volume is a continuation of Volume 1 following the previously published Editorial. More emphasis is given to novel nanocarrier designs, their characterization and function, and applications for drug discovery and treatment. A number of chapters will deal with nanofibers as a new major application within the biomedical field with a very high success rate particularly in wound healing and diabetic foot and spine injuries. A major new subdivision will deal with mathematical methods for the assembly of nanocarriers both for simulation and function.

Author : Ales Prokop
Publisher : Springer
Page : 867 pages
File Size : 46,7 Mb
Release : 2011-08-27
Category : Science
ISBN : 9400712499

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Intracellular Delivery by Ales Prokop Pdf

This book features a special subsection of Nanomedicine, an application of nanotechnology to achieve breakthroughs in healthcare. It exploits the improved and often novel physical, chemical and biological properties of materials only existent at the nanometer scale. As a consequence of small scale, nanosystems in most cases are efficiently uptaken by cells and appear to act at the intracellular level. Nanotechnology has the potential to improve diagnosis, treatment and follow-up of diseases, and includes targeted drug delivery and regenerative medicine; it creates new tools and methods that impact significantly upon existing conservative practices. This volume is a collection of authoritative reviews. In the introductory section we define the field (intracellular delivery). Then, the fundamental routes of nanodelivery devices, cellular uptake, types of delivery devices, particularly in terms of localized cellular delivery, both for small drug molecules, macromolecular drugs and genes; at the academic and applied levels, are covered. The following section is dedicated to enhancing delivery via special targeting motifs followed by the introduction of different types of intracellular nanodelivery devices (e.g. a brief description of their chemistry) and ways of producing these different devices. Finally, we put special emphasis on particular disease states and on other biomedical applications, whilst diagnostic and sensing issues are also included. Intracellular delivery / therapy is a highly topical which will stir great interest. Intracellular delivery enables much more efficient drug delivery since the impact (on different organelles and sites) is intracellular as the drug is not supplied externally within the blood stream. There is great potential for targeted delivery with improved localized delivery and efficacy.

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 51,6 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

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Molecular Biology of The Cell by Bruce Alberts Pdf

Author : D. Robert Lu,Svein Øie
Publisher : Springer Science & Business Media
Page : 621 pages
File Size : 45,8 Mb
Release : 2004-02-25
Category : Medical
ISBN : 9781592597451

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Cellular Drug Delivery by D. Robert Lu,Svein Øie Pdf

An authoritative and up-to-date survey of the fundamental principles, and practice of drug delivery at the cellular level. On the principles side, the authors discuss the broad spectrum of cellular delivery, ranging from coverage of cell-mediated immunity, gene delivery, and protein targeting, to cellular drug transport, cellular drug permeability, and a variety of carrier system related to targeted drug delivery. On the practice side, the authors focus on technological developments in cellular drug delivery, including novel formulations for the delivery of DNA and antisense oligonucleotides ,as well as drug targeting with immunoglobulin formulations and antibody-mediated approaches.

Author : Ji Zhu
Publisher : Unknown
Page : 510 pages
File Size : 52,5 Mb
Release : 2000
Category : Electronic
ISBN : UCAL:X60727

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Strategies to Improve Liposome-mediated Intracellular Delivery of Polynucleotides by Ji Zhu Pdf

Author : Ngoc-Anh Thi Nguyen
Publisher : Unknown
Page : 418 pages
File Size : 48,6 Mb
Release : 2000
Category : Electronic
ISBN : UOM:39015049507851

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Intracellular Delivery of Antisense Oligodeoxynucleotides Utilizing Starburst Polyamidoamine (PAMAM) Dendrimers as Carriers by Ngoc-Anh Thi Nguyen Pdf

Author : Kishor M. Wasan
Publisher : John Wiley & Sons
Page : 217 pages
File Size : 54,8 Mb
Release : 2007-01-09
Category : Science
ISBN : 9780470097977

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Role of Lipid Excipients in Modifying Oral and Parenteral Drug Delivery by Kishor M. Wasan Pdf

This comprehensive resource covers the fundamentals, formulation, and biopharmaceutical issues of lipid-based drug delivery. It presents the principles of lipid absorption and covers formulation issues, such as dissolution testing and stability testing, and physiological and biopharmaceutical issues, including the role of specific enzymes, the evaluation of transport systems in the body, and the mechanisms governing the transport of water-insoluble drugs.

Author : Nava Segev
Publisher : Springer Science & Business Media
Page : 459 pages
File Size : 52,5 Mb
Release : 2010-05-30
Category : Science
ISBN : 9780387938776

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Trafficking Inside Cells by Nava Segev Pdf

This book covers the past, present and future of the intra-cellular trafficking field, which has made a quantum leap in the last few decades. It details how the field has developed and evolved as well as examines future directions.

Author : Baldassare Di Bartolo,John Collins,Luciano Silvestri
Publisher : Springer
Page : 584 pages
File Size : 53,6 Mb
Release : 2017-02-15
Category : Science
ISBN : 9789402408508

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Nano-Optics: Principles Enabling Basic Research and Applications by Baldassare Di Bartolo,John Collins,Luciano Silvestri Pdf

This book provides a comprehensive overview of nano-optics, including basic theory, experiment and applications, particularly in nanofabrication and optical characterization. The contributions clearly demonstrate how advances in nano-optics and photonics have stimulated progress in nanoscience and -fabrication, and vice versa. Their expert authors address topics such as three-dimensional optical lithography and microscopy beyond the Abbe diffraction limit, optical diagnostics and sensing, optical data- and telecommunications, energy-efficient lighting, and efficient solar energy conversion. Nano-optics emerges as a key enabling technology of the 21st century. This work will appeal to a wide readership, from physics through chemistry, to biology and engineering. The contributions that appear in this volume were presented at a NATO Advanced Study Institute held in Erice, 4-19 July, 2015. Re Ch. 73 - Structure and Luminescence Properties of Nanofluorapatite Activated with Eu3+ Ions Synthesized by Hydrothermal Method, pp 567-569: The authors would like to acknowledge the National Science Centre (NSC) for financial support within the Project ‘Preparation and characterization of nanoapatites doped with rare earth ions and their biocomposites’ UMO-2012/05/E/ST5/03904

Author : Robert Vink,Mihai Nechifor
Publisher : University of Adelaide Press
Page : 354 pages
File Size : 52,9 Mb
Release : 2011
Category : Medical
ISBN : 9780987073051

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Magnesium in the Central Nervous System by Robert Vink,Mihai Nechifor Pdf

The brain is the most complex organ in our body. Indeed, it is perhaps the most complex structure we have ever encountered in nature. Both structurally and functionally, there are many peculiarities that differentiate the brain from all other organs. The brain is our connection to the world around us and by governing nervous system and higher function, any disturbance induces severe neurological and psychiatric disorders that can have a devastating effect on quality of life. Our understanding of the physiology and biochemistry of the brain has improved dramatically in the last two decades. In particular, the critical role of cations, including magnesium, has become evident, even if incompletely understood at a mechanistic level. The exact role and regulation of magnesium, in particular, remains elusive, largely because intracellular levels are so difficult to routinely quantify. Nonetheless, the importance of magnesium to normal central nervous system activity is self-evident given the complicated homeostatic mechanisms that maintain the concentration of this cation within strict limits essential for normal physiology and metabolism. There is also considerable accumulating evidence to suggest alterations to some brain functions in both normal and pathological conditions may be linked to alterations in local magnesium concentration. This book, containing chapters written by some of the foremost experts in the field of magnesium research, brings together the latest in experimental and clinical magnesium research as it relates to the central nervous system. It offers a complete and updated view of magnesiums involvement in central nervous system function and in so doing, brings together two main pillars of contemporary neuroscience research, namely providing an explanation for the molecular mechanisms involved in brain function, and emphasizing the connections between the molecular changes and behavior. It is the untiring efforts of those magnesium researchers who have dedicated their lives to unraveling the mysteries of magnesiums role in biological systems that has inspired the collation of this volume of work.

Author : Seiichi Uchiyama
Publisher : John Wiley & Sons
Page : 357 pages
File Size : 48,5 Mb
Release : 2024-04-03
Category : Science
ISBN : 9783527836857

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Intracellular Thermometry with Fluorescent Molecular Thermometers by Seiichi Uchiyama Pdf

Intracellular Thermometry with Fluorescent Molecular Thermometers Understand a vital new bioanalytical technique with this comprehensive introduction to measuring temperature on the cellular scale Most organisms have highly controlled body temperatures, fluctuations in which are therefore sensitive indicators of changes in body function. In recent years, the development of fluorescent molecular thermometers and related intracellular temperature probes has enabled researchers to track these fluctuations at the cellular rather than the organismic level, opening up a whole new field of study in cell and molecular biology. Intracellular Thermometry with Fluorescent Molecular Thermometers provides bioanalytical researchers with an introduction to these technologies and their current and future applications. Starting off with a discussion of temperature as a key factor in biological regulation, it provides an authoritative overview of available fluorescent temperature probes, their characteristics and potential applications. Intracellular Thermometry with Fluorescent Molecular Thermometers readers will also find: Step by step instructions for constructing an intracellular thermometry experiment and validating results Comprehensive discussion of existing applications A vision for the future development of thermal biology as an independent discipline Authored by a pioneer in the field of intracellular thermometry, Intracellular Thermometry with Fluorescent Molecular Thermometers is ideal for researchers in analytical chemistry, cell biology, molecular biology, biophysics, or any related subjects.

Author : Eugene Vorobiev,Nikolai Lebovka
Publisher : Springer Science & Business Media
Page : 286 pages
File Size : 45,6 Mb
Release : 2009-02-28
Category : Technology & Engineering
ISBN : 9780387793740

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Electrotechnologies for Extraction from Food Plants and Biomaterials by Eugene Vorobiev,Nikolai Lebovka Pdf

Recently, the electrotechnologies based on the effects of pulsed electric fields (PEF), such as ohmic heating (OH) and DC electric field, have gained real interest in the field of food processing. These techniques efficiently enhance methods of extraction from food plants and dehydration of biosolids. The PEF and pulsed OH techniques preserve the nutritional, functional, structural and sensory properties of products better than conventional extraction technologies. The electrofiltration and electro-osmotic dewatering can be very effective for the separation of bioproducts and dehydration of food wastes. The first source book in the field, this book gives an overview the fundamental principles of electrical techniques, electrophysical properties of foods and agricultural products, application of various emerging electrotechnologies for enhancing the solid-liquid separation and drying processes, extraction techniques of pigments, processing methods of different in-plant tissues and biosolids, electro-osmotic dewatering and electrofiltration of biomaterials, recent industrial- scale gains, and other aspects. Each chapter is complementary to other chapters and addresses the latest efforts in the field.