Label Free Technologies For Drug Discovery

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Label-Free Technologies For Drug Discovery

Matthew Cooper,Lorenz M. Mayr
Label Free Technologies For Drug Discovery Book PDF

Author : Matthew Cooper,Lorenz M. Mayr
Publisher : John Wiley & Sons
Page : 357 pages
File Size : 55,7 Mb
Release : 2011-02-11
Category : Medical
ISBN : 9781119990277

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Label-Free Technologies For Drug Discovery by Matthew Cooper,Lorenz M. Mayr Pdf

Over the past two decades the benefits of label-free biosensor analysis have begun to make an impact in the market, and systems are beginning to be used as mainstream research tools in many drug discovery laboratories. Label-Free Technologies For Drug Discovery summarises the latest and emerging developments in label-free detection systems, their underlying technology principles and end-user case studies that reveal the power and limitations of label-free in all areas of drug discovery. Label-free technologies discussed include SPR, NMR, high-throughput mass spectrometry, resonant waveguide plate-based screening, transmitted-light imaging, isothermal titration calorimetry, optical and impedance cell-based assays and other biophysical methods. The technologies are discussed in relation to their use as screening technologies, high-content technologies, hit finding and hit validation strategies, mode of action and ADME/T, access to difficult target classes, cell-based receptor/ligand interactions particularly orphan receptors, and antibody and small molecule affinity and kinetic analysis. Label-Free Technologies For Drug Discovery is an essential guide to this emerging class of tools for researchers in drug discovery and development, particularly high-throughput screening and compound profiling teams, medicinal chemists, structural biologists, assay developers, ADME/T specialists, and others interested in biomolecular interaction analysis.

Label-Free Biosensor Methods in Drug Discovery

Ye Fang
Label Free Biosensor Methods in Drug Discovery Book PDF

Author : Ye Fang
Publisher : Humana
Page : 0 pages
File Size : 48,8 Mb
Release : 2016-10-05
Category : Medical
ISBN : 1493949616

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Label-Free Biosensor Methods in Drug Discovery by Ye Fang Pdf

This volume explores label-free biosensors, advantageous in part because this technology bypasses the need of labels, reporters, and cell engineering, all of which are common to labeled techniques but may introduce artifacts in assay results. Addressing several fundamental and practical aspects as to how to implement label-free methods in the drug discovery process, this book covers a wide range of topics, including binding kinetics determination, fragment screening, antibody epitope mapping, protein-protein interaction profiling and screening, receptor pathway deconvolution, drug pharmacology profiling and screening, target identification, drug toxicity assessment, and physical phenotype profiling and diagnostics based on various cellular processes such as cell adhesion, migration, invasion, infection, and inflammation. As part of the Methods in Pharmacology and Toxicology series, chapters aim to provide key detail and implementation advice to aid with progress in the lab. Practical and thorough, Label-Free Biosensor Methods in Drug Discovery provides a new avenue for rapid access to a focused collection of highly regarded contributions in the field.

Label-Free Biosensors

Matthew A. Cooper
Label Free Biosensors Book PDF

Author : Matthew A. Cooper
Publisher : Cambridge University Press
Page : 317 pages
File Size : 48,6 Mb
Release : 2009-02-02
Category : Medical
ISBN : 9780521884532

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Label-Free Biosensors by Matthew A. Cooper Pdf

A detailed technical review of label-free biosensor techniques with worked examples.

Label-Free Monitoring of Cells in vitro

Joachim Wegener
Label Free Monitoring of Cells in vitro Book PDF

Author : Joachim Wegener
Publisher : Springer Nature
Page : 286 pages
File Size : 49,6 Mb
Release : 2019-11-18
Category : Science
ISBN : 9783030324339

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Label-Free Monitoring of Cells in vitro by Joachim Wegener Pdf

This book is dedicated to label-free, non-invasive monitoring of cell-based assays and it comprises the most widely applied techniques. Each approach is described and critically evaluated by an expert in the field such that researchers get an overview on what is possible and where the limitations are. The book provides the theoretical basis for each technique as well as the most successful and exciting applications. Label-free bioanalytical techniques have been known for a long time as valuable tools to monitor adsorption processes at the solid-liquid interface in general – and biomolecular interaction analysis (BIA) in particular. The underlying concepts have been progressively transferred to the analysis of cell-based assays. The strength of these approaches is implicitly given with the name 'label-free': the readout is independent of any label, reagent or additive that contaminates the system under study and potentially affects its properties. Thus, label-free techniques provide an unbiased analytical perspective in the sense that the sample is not manipulated by additives but pure. They are commonly based on physical principles and read changes in integral physical properties of the sample like refractive index, conductivity, capacitance or elastic modulus to mention just a few. Even though it is not implied in the name, label-free approaches usually monitor the cells under study non-invasively meaning that the amplitude of the signal (e.g. electric field strength, mechanical elongation) that is used for the measurement is too low to interfere or affect. In contrast to label-based analytical techniques that are commonly restricted to a single reading at a predefined time point, label-free approaches allow for a continuous observation so that the dynamics of the biological system or reaction become accessible.

Label-free and Multi-parametric Monitoring of Cell-based Assays with Substrate-embedded Sensors

Maximilian Oberleitner
Label free and Multi parametric Monitoring of Cell based Assays with Substrate embedded Sensors Book PDF

Author : Maximilian Oberleitner
Publisher : Springer
Page : 370 pages
File Size : 44,9 Mb
Release : 2017-09-26
Category : Science
ISBN : 9783319453842

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Label-free and Multi-parametric Monitoring of Cell-based Assays with Substrate-embedded Sensors by Maximilian Oberleitner Pdf

This thesis describes novel substrate embedded physical sensors that can be used to monitor different types of cell-based assays non-invasively and label-free. The sensors described provide integrative information of the cells under study with an adaptable time resolution (ranging from milliseconds to days). This information about the dynamic cell response to chemical, physical or biological stimuli defines a new paradigm in fundamental biomedical research. The author, Maximilian Oberleitner, describes approaches in which the cells are directly grown on different sensor surfaces (gold-film electrodes, shear wave resonators or dye-doped polymer films). This approach, with the reacting cells in particularly close proximity and contact with the sensor surface, is key to a remarkable sensitivity, opening the way for a variety of new applications. This thesis not only introduces the fundamentals of each approach, but it also describes in great detail the design principles and elucidates the boundary conditions of the new sensors.

Oligomerization and Allosteric Modulation in G-Protein Coupled Receptors

Anonim
Oligomerization and Allosteric Modulation in G Protein Coupled Receptors Book PDF

Author : Anonim
Publisher : Academic Press
Page : 495 pages
File Size : 41,5 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780123947918

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Oligomerization and Allosteric Modulation in G-Protein Coupled Receptors by Anonim Pdf

In this thematic volume of Progress in Molecular Biology and Translational Science, researchers reflect on recent developments and research surrounding G protein-coupled receptors. The chapters cover a large breadth of research, including GPCR role in stem cell function and pharmacology. Authors explore in-depth research techniques and applications of GPCR usage, covering theory, laboratory approaches, and unique qualities that make GPCRs a crucial tool in microbiological and cancer research. Contributions from leading authorities Informs and updates on all the latest developments in the field

Label-Free Biosensors

Matthew A. Cooper
Label Free Biosensors Book PDF

Author : Matthew A. Cooper
Publisher : Cambridge University Press
Page : 305 pages
File Size : 53,6 Mb
Release : 2009-02-02
Category : Science
ISBN : 9781139476782

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Label-Free Biosensors by Matthew A. Cooper Pdf

Label-free biosensors are devices that use biological or chemical receptors to detect analytes (molecules) in a sample. They give detailed information on the selectivity, affinity, and, in many cases, also the binding kinetics and thermodynamics of an interaction. Although they can be powerful tools in the hands of a skilled user, there is often a lack of knowledge of the best methods for using label-free assays to screen for biologically active molecules and accurately and precisely characterize molecular recognition events. This book reviews both established and newer label-free techniques giving both the expert user and the general reader interested in the technologies and applications behind label-free an insight into the field from expert opinion leaders and practitioners of the technologies. Most importantly, chapters contain worked examples from leaders in the field that take the reader through the basics of experimental design, setup, assay development, and data analysis.

Handbook of Drug Screening

Ramakrishna Seethala,Litao Zhang
Handbook of Drug Screening Book PDF

Author : Ramakrishna Seethala,Litao Zhang
Publisher : CRC Press
Page : 504 pages
File Size : 51,8 Mb
Release : 2016-04-19
Category : Medical
ISBN : 9781420061697

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Handbook of Drug Screening by Ramakrishna Seethala,Litao Zhang Pdf

Building upon the foundation of basics discussed in the previous edition, the Second Edition provides a more in-depth look at the latest methods and technologies of advanced drug screening, an essential function of drug discovery. With extensively updated content and 21 new chapters, this text examines:quality and efficiency of drug target validati

Basic Principles of Drug Discovery and Development

Benjamin E. Blass
Basic Principles of Drug Discovery and Development Book PDF

Author : Benjamin E. Blass
Publisher : Academic Press
Page : 712 pages
File Size : 41,6 Mb
Release : 2021-03-30
Category : Medical
ISBN : 9780128172155

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Basic Principles of Drug Discovery and Development by Benjamin E. Blass Pdf

Basic Principles of Drug Discovery and Development presents the multifaceted process of identifying a new drug in the modern era, which requires a multidisciplinary team approach with input from medicinal chemists, biologists, pharmacologists, drug metabolism experts, toxicologists, clinicians, and a host of experts from numerous additional fields. Enabling technologies such as high throughput screening, structure-based drug design, molecular modeling, pharmaceutical profiling, and translational medicine are critical to the successful development of marketable therapeutics. Given the wide range of disciplines and techniques that are required for cutting edge drug discovery and development, a scientist must master their own fields as well as have a fundamental understanding of their collaborator’s fields. This book bridges the knowledge gaps that invariably lead to communication issues in a new scientist’s early career, providing a fundamental understanding of the various techniques and disciplines required for the multifaceted endeavor of drug research and development. It provides students, new industrial scientists, and academics with a basic understanding of the drug discovery and development process. The fully updated text provides an excellent overview of the process and includes chapters on important drug targets by class, in vitro screening methods, medicinal chemistry strategies in drug design, principles of in vivo pharmacokinetics and pharmacodynamics, animal models of disease states, clinical trial basics, and selected business aspects of the drug discovery process. Provides a clear explanation of how the pharmaceutical industry works, as well as the complete drug discovery and development process, from obtaining a lead, to testing the bioactivity, to producing the drug, and protecting the intellectual property Includes a new chapter on the discovery and development of biologics (antibodies proteins, antibody/receptor complexes, antibody drug conjugates), a growing and important area of the pharmaceutical industry landscape Features a new section on formulations, including a discussion of IV formulations suitable for human clinical trials, as well as the application of nanotechnology and the use of transdermal patch technology for drug delivery Updated chapter with new case studies includes additional modern examples of drug discovery through high through-put screening, fragment-based drug design, and computational chemistry

Biosensors and Nanotechnology

Zeynep Altintas
Biosensors and Nanotechnology Book PDF

Author : Zeynep Altintas
Publisher : John Wiley & Sons
Page : 421 pages
File Size : 51,9 Mb
Release : 2017-12-18
Category : Technology & Engineering
ISBN : 9781119065012

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Biosensors and Nanotechnology by Zeynep Altintas Pdf

Provides a broad range of information from basic principles to advanced applications of biosensors and nanomaterials in health care diagnostics This book utilizes a multidisciplinary approach to provide a wide range of information on biosensors and the impact of nanotechnology on the development of biosensors for health care. It offers a solid background on biosensors, recognition receptors, biomarkers, and disease diagnostics. An overview of biosensor-based health care applications is addressed. Nanomaterial applications in biosensors and diagnostics are included, covering the application of nanoparticles, magnetic nanomaterials, quantum dots, carbon nanotubes, graphene, and molecularly imprinted nanostructures. The topic of organ-specific health care systems utilizing biosensors is also incorporated to provide deep insight into the very recent advances in disease diagnostics. Biosensors and Nanotechnology: Applications in Health Care Diagnostics is comprised of 15 chapters that are presented in four sections and written by 33 researchers who are actively working in Germany, the United Kingdom, Italy, Turkey, Denmark, Finland, Romania, Malaysia and Brazil. It covers biomarkers in healthcare; microfluidics in medical diagnostics; SPR-based biosensor techniques; piezoelectric-based biosensor technologies; MEMS-based cell counting methods; lab-on-chip platforms; optical applications for cancer cases; and more. Discusses the latest technology and advances in the field of biosensors and their applications for healthcare diagnostics Particular focus on biosensors for cancer Summarizes research of the last 30 years, relating it to state-of-the-art technologies Biosensors and Nanotechnology: Applications in Health Care Diagnostics is an excellent book for researchers, scientists, regulators, consultants, and engineers in the field, as well as for graduate students studying the subject.

Integrated Drug Discovery Technologies

Houng-Yau Mei,Anthony W. Czarnik
Integrated Drug Discovery Technologies Book PDF

Author : Houng-Yau Mei,Anthony W. Czarnik
Publisher : CRC Press
Page : 576 pages
File Size : 46,8 Mb
Release : 2002-03-19
Category : Medical
ISBN : 9780203910696

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Integrated Drug Discovery Technologies by Houng-Yau Mei,Anthony W. Czarnik Pdf

Integrated Drug Discovery Technologies provides a global overview of emerging drug development technologies by presenting and integrating new techniques from the disciplines of chemistry, biology, and computational sciences. It combines integration of contemporary mechanization with strategies in drug delivery. Topics include: functional genomics,

Materials Research for Manufacturing

Lynnette D Madsen,Erik B Svedberg
Materials Research for Manufacturing Book PDF

Author : Lynnette D Madsen,Erik B Svedberg
Publisher : Springer
Page : 338 pages
File Size : 47,5 Mb
Release : 2016-01-14
Category : Technology & Engineering
ISBN : 9783319234199

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Materials Research for Manufacturing by Lynnette D Madsen,Erik B Svedberg Pdf

This book is about applied materials research in industry. It presents various important topics and challenges and gives guidance to materials researchers who move to industry. The book focuses on the materials manufacturing issues for industrial application. It deals with developments and challenges in traditional materials areas, such as metals and ceramics, and new opportunities that have risen from nanotechnology and additive manufacturing. The chapters, written by senior people from large companies, include successful manufacturing undertakings, several distinct and unresolved manufacturing challenges, with the focus on approaches, timelines and the skills needed for future company research and development. The book provides a cross-section of current and future approaches valuable for new employees and academics working in industry.

Handbook of Spectroscopy

G¿nter Gauglitz,David S. Moore
Handbook of Spectroscopy Book PDF

Author : G¿nter Gauglitz,David S. Moore
Publisher : John Wiley & Sons
Page : 2011 pages
File Size : 48,5 Mb
Release : 2014-06-09
Category : Science
ISBN : 9783527321506

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Handbook of Spectroscopy by G¿nter Gauglitz,David S. Moore Pdf

This second, thoroughly revised, updated and enlarged edition provides a straightforward introduction to spectroscopy, showing what it can do and how it does it, together with a clear, integrated and objective account of the wealth of information that may be derived from spectra. It also features new chapters on spectroscopy in nano-dimensions, nano-optics, and polymer analysis. Clearly structured into sixteen sections, it covers everything from spectroscopy in nanodimensions to medicinal applications, spanning a wide range of the electromagnetic spectrum and the physical processes involved, from nuclear phenomena to molecular rotation processes. In addition, data tables provide a comparison of different methods in a standardized form, allowing readers to save valuable time in the decision process by avoiding wrong turns, and also help in selecting the instrumentation and performing the experiments. These four volumes are a must-have companion for daily use in every lab.

G Protein-Coupled Receptors

Anonim
G Protein Coupled Receptors Book PDF

Author : Anonim
Publisher : Academic Press
Page : 332 pages
File Size : 54,7 Mb
Release : 2019-01-05
Category : Science
ISBN : 9780128151082

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G Protein-Coupled Receptors by Anonim Pdf

G-Protein-Coupled Receptors, Part B, 2nd Edition, Volume 149, the latest release in the Methods in Cell Biology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers Optical Approaches for Visualization of Arrestin Binding to Muscarinic Receptors, Luciferase Reporter Assay for Unlocking Ligand-mediated Signaling of GPCRs, Assays to Measure GPCR Dependent Cellular Migration, Characterization of the Frizzled GPCRs, Binding Assays for Bradykinin and Angiotensin Receptors, Detection of Misfolded Rhodopsin Aggregates in Cells, Measuring GPCR Ubiquitination and Trafficking, Culture of Primary Neurons and its Use in Studying GPCR Trafficking, and much more. Covers the increasingly appreciated cell biology field of G-protein-coupled receptors Includes both established and new technologies Contributed by experts in the field

Biosensors and Molecular Technologies for Cancer Diagnostics

Keith E. Herold,Avraham Rasooly
Biosensors and Molecular Technologies for Cancer Diagnostics Book PDF

Author : Keith E. Herold,Avraham Rasooly
Publisher : CRC Press
Page : 847 pages
File Size : 53,7 Mb
Release : 2012-05-29
Category : Science
ISBN : 9781439841655

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Biosensors and Molecular Technologies for Cancer Diagnostics by Keith E. Herold,Avraham Rasooly Pdf

Bridging the gap between research and clinical application, Biosensors and Molecular Technologies for Cancer Diagnostics explores the use of biosensors as effective alternatives to the current standard methods in cancer diagnosis and detection. It describes the major aspects involved in detecting and diagnosing cancer as well as the basic elements of biosensors and their applications in detection and diagnostics. The book addresses cancer molecular diagnostics, including genomic and proteomic approaches, from the perspective of biosensors and biodetection. It explains how to measure and understand molecular markers using biosensors and discusses the medical advantages of rapid and accurate cancer diagnostics. It also describes optical, electrochemical, and optomechanical biosensor technologies, with a focus on cancer analysis and the clinical utility of these technologies for cancer detection, diagnostics, prognostics, and treatment. Making biosensor technology more accessible to molecular biologists, oncologists, pathologists, and engineers, this volume advances the integration of this technology into mainstream clinical practice. Through its in-depth coverage of a range of biosensors, the book shows how they can play instrumental roles in the early molecular diagnosis of cancer.