Laboratory Protocols In Applied Life Sciences

Author : Prakash Singh Bisen
Publisher : CRC Press
Page : 1812 pages
File Size : 41,7 Mb
Release : 2014-02-26
Category : Medical
ISBN : 9781466553149

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Laboratory Protocols in Applied Life Sciences by Prakash Singh Bisen Pdf

As applied life science progresses, becoming fully integrated into the biological, chemical, and engineering sciences, there is a growing need for expanding life sciences research techniques. Anticipating the demands of various life science disciplines, Laboratory Protocols in Applied Life Sciences explores this development. This book covers a wide spectrum of areas in the interdisciplinary fields of life sciences, pharmacy, medical and paramedical sciences, and biotechnology. It examines the principles, concepts, and every aspect of applicable techniques in these areas. Covering elementary concepts to advanced research techniques, the text analyzes data through experimentation and explains the theory behind each exercise. It presents each experiment with an introduction to the topic, concise objectives, and a list of necessary materials and reagents, and introduces step-by-step, readily feasible laboratory protocols. Focusing on the chemical characteristics of enzymes, metabolic processes, product and raw materials, and on the basic mechanisms and analytical techniques involved in life science technological transformations, this text provides information on the biological characteristics of living cells of different origin and the development of new life forms by genetic engineering techniques. It also examines product development using biological systems, including pharmaceutical, food, and beverage industries. Laboratory Protocols in Applied Life Sciences presents a nonmathematical account of the underlying principles of a variety of experimental techniques in disciplines, including: Biotechnology Analytical biochemistry Clinical biochemistry Biophysics Molecular biology Genetic engineering Bioprocess technology Industrial processes Animal Plant Microbial biology Computational biology Biosensors Each chapter is self-contained and written in a style that helps students progress from basic to advanced techniques, and eventually design and execute their own experiments in a given field of biology.

Author : PRAKASH SINGH. BISEN
Publisher : Unknown
Page : 128 pages
File Size : 54,6 Mb
Release : 2018
Category : Electronic
ISBN : 1138071951

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LABORATORY PROTOCOLS IN APPLIED LIFE SCIENCES. by PRAKASH SINGH. BISEN Pdf

Author : Christopher Panaretos
Publisher : Independently Published
Page : 31 pages
File Size : 54,8 Mb
Release : 2019-06-23
Category : Electronic
ISBN : 1075696836

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Biology Laboratory Protocols by Christopher Panaretos Pdf

This short, 30 page book gives a brief and generalized overview of the most common concepts in biology laboratory research. Standard laboratory protocols utilize these concepts in a modular fashion. Understanding this modular nature enables you to design custom protocols to collect data from arbitrary biological systems.

Author : Sean R. Gallagher,Emily A. Wiley
Publisher : John Wiley & Sons
Page : 679 pages
File Size : 52,5 Mb
Release : 2012-03-19
Category : Science
ISBN : 9780470942413

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Current Protocols Essential Laboratory Techniques by Sean R. Gallagher,Emily A. Wiley Pdf

The latest title from the acclaimed Current Protocols series, Current Protocols Essential Laboratory Techniques, 2e provides the new researcher with the skills and understanding of the fundamental laboratory procedures necessary to run successful experiments, solve problems, and become a productive member of the modern life science laboratory. From covering the basic skills such as measurement, preparation of reagents and use of basic instrumentation to the more advanced techniques such as blotting, chromatography and real-time PCR, this book will serve as a practical reference manual for any life science researcher. Written by a combination of distinguished investigators and outstanding faculty, Current Protocols Essential Laboratory Techniques, 2e is the cornerstone on which the beginning scientist can develop the skills for a successful research career.

Author : Prakash Singh Bisen,Anjana Sharma
Publisher : CRC Press
Page : 387 pages
File Size : 49,6 Mb
Release : 2012-09-26
Category : Medical
ISBN : 9781466512405

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Introduction to Instrumentation in Life Sciences by Prakash Singh Bisen,Anjana Sharma Pdf

Instrumentation is central to the study of physiology and genetics in living organisms, especially at the molecular level. Numerous techniques have been developed to address this in various biological disciplines, creating a need to understand the physical principles involved in the operation of research instruments and the parameters required in using them. Introduction to Instrumentation in Life Sciences fills this need by addressing different aspects of tools that hold the keys to cutting-edge research and innovative applications, from basic techniques to advanced instrumentation. The text describes all topics so even beginners can easily understand the theoretical and practical aspects. Comprehensive chapters encompass well-defined methodology that describes the instruments and their corresponding applications in different scientific fields. The book covers optical and electron microscopy; micrometry, especially in microbial taxonomy; pH meters and oxygen electrodes; chromatography for separation and purification of products from complex mixtures; spectroscopic and spectrophotometric techniques to determine structure and function of biomolecules; preparative and analytical centrifugation; electrophoretic techniques; x-ray microanalysis including crystallography; applications of radioactivity, including autoradiography and radioimmunoassays; and fermentation technology and subsequent separation of products of interest. The book is designed to serve a wide range of students and researchers in diversified fields of life sciences: pharmacy, biotechnology, microbiology, biochemistry, and environmental sciences. It introduces different aspects of basic experimental methods and instrumentation. The book is unique in its broad subject coverage, incorporating fundamental techniques as well as applications of modern molecular and proteomic tools that are the basis for state-of-the-art research. The text emphasizes techniques encountered both in practical classes and in high-throughput environments used in modern industry. As a further aid to students, the authors provide well-illustrated diagrams to explain the principles and theories behind the instruments described.

Author : Steven A. Williams,Barton Elliott Slatko,John R. McCarrey
Publisher : Jones & Bartlett Learning
Page : 272 pages
File Size : 53,8 Mb
Release : 2007
Category : Science
ISBN : 0763733296

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Laboratory Investigations in Molecular Biology by Steven A. Williams,Barton Elliott Slatko,John R. McCarrey Pdf

Laboratory Investigations in Molecular Biology presents well-tested protocols in molecular biology that are commonly used in currently active research labs. It is an ideal laboratory manual for college level courses in molecular biology. Because of the modular organization of the manual, laboratory courses can be assembled that would be ideal for science professionals, graduate students, undergraduate students and even advanced high school students in AP courses. The manual is also intended to be useful as a laboratory "bench reference". The experiments are designed to guide students through realistic research projects and to provide students with instruction in methods and approaches that can be immediately translated into research projects conducted in modern research laboratories. Although these experiments have been conducted and optimized over 20 years of teaching the New England Biolabs Molecular Biology Summer Workshops, they are real research projects, not "canned" experiments. Based on extensive teaching experience using these protocols, the authors have found that conducting these experiments as described in these protocols serves to effectively instruct students and science professions in the basic methods of molecular biology. An additional unique feature is that the protocols described in the manual are accompanied by available reagent kits that provide quality-tested, pre-packaged reagents to ensure the successful application of these protocols in a laboratory course setting.

Author : Michael Kaufmann,Claudia Klinger
Publisher : Humana Press
Page : 418 pages
File Size : 43,6 Mb
Release : 2011-12-02
Category : Medical
ISBN : 1617794236

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Functional Genomics by Michael Kaufmann,Claudia Klinger Pdf

Over the last decade Life Science has undergone an accelerated evolution, culminating in the -omics era characterized by the development of a multitude of high throughput methods that are becoming more routinely applied in biochemistry labs. In Functional Genomics: Methods and Protocols, Second Edition expert researchers in the field detail many of the methods which are now commonly used for studies in the life sciences focusing on the dynamic aspects of the transcriptome, proteome and metabolome, respectively.Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Functional Genomics: Methods and Protocols, Second Edition seeks to aid scientists in establishing or extending technologies and techniques in their laboratories.

Author : Vijai Kumar Gupta,Maria G. Tuohy,Manimaran Ayyachamy,Kevin M. Turner,Anthonia O’Donovan
Publisher : Springer Science & Business Media
Page : 604 pages
File Size : 54,8 Mb
Release : 2012-12-09
Category : Science
ISBN : 9781461423560

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Laboratory Protocols in Fungal Biology by Vijai Kumar Gupta,Maria G. Tuohy,Manimaran Ayyachamy,Kevin M. Turner,Anthonia O’Donovan Pdf

Laboratory Protocols in Fungal Biology presents the latest techniques in fungal biology. This book analyzes information derived through real experiments, and focuses on cutting edge techniques in the field. The book comprises 57 chapters contributed from internationally recognised scientists and researchers. Experts in the field have provided up-to-date protocols covering a range of frequently used methods in fungal biology. Almost all important methods available in the area of fungal biology viz. taxonomic keys in fungi; histopathological and microscopy techniques; proteomics methods; genomics methods; industrial applications and related techniques; and bioinformatics tools in fungi are covered and complied in one book. Chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting. Each chapter is self-contained and written in a style that enables the reader to progress from elementary concepts to advanced research techniques. Laboratory Protocols in Fungal Biology is a valuable tool for both beginner research workers and experienced professionals. Coming Soon in the Fungal Biology series: Goyal, Manoharachary / Future Challenges in Crop Protection Against Fungal Pathogens Martín, García-Estrada, Zeilinger / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites Zeilinger, Martín, García-Estrada / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites, Volume 2 van den Berg, Maruthachalam / Genetic Transformation Systems in Fungi Schmoll, Dattenbock / Gene Expression Systems in Fungi Dahms / Advanced Microscopy in Mycology

Author : Lucília Domingues
Publisher : Springer
Page : 282 pages
File Size : 52,9 Mb
Release : 2018-08-08
Category : Science
ISBN : 1493983814

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PCR by Lucília Domingues Pdf

This volume details PCR technique with focus on its application specificities to the biotechnology and bioengineering field. Chapters are broken into five sections covering sgeneral PCR protocols, different applied examples to molecular and synthetic biotechnology, food science and technology, environmental microbiology and molecular ecology, and healthcare. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, PCR: Methods and Protocols hopes to be a valuable and useful resource for wet lab researchers, particularly within the biotechnology and bioengineering field.

Author : Sandeep Singh,Dhiraj Kumar
Publisher : Bentham Science Publishers
Page : 186 pages
File Size : 55,6 Mb
Release : 2020-01-23
Category : Science
ISBN : 9789811439292

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Protocols used in Molecular Biology by Sandeep Singh,Dhiraj Kumar Pdf

Protocols used in Molecular Biology is a compilation of several examples of molecular biology protocols. Each example is presented with a concise introduction, materials and chemicals required, a step-by-step procedure and troubleshooting tips. Information about the application of the protocol is also provided. The techniques included in this book are essential to research in the fields of proteomics, genomics, cell culture, epigenetic modification and structural biology. The protocols can also be used by clinical researchers (neuroscientists and oncologists, for example) for medical applications (diagnostics, therapeutics and multidisciplinary projects).

Author : Mathieu Vinken,Scott R. Johnstone
Publisher : Methods in Molecular Biology
Page : 252 pages
File Size : 48,5 Mb
Release : 2018-06-04
Category : Science
ISBN : 1493981102

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Gap Junction Protocols by Mathieu Vinken,Scott R. Johnstone Pdf

Corrected statement of responsibility may be: edited by ...

Author : Kira Astakhova,Syeda Atia Bukhari
Publisher : Humana
Page : 0 pages
File Size : 54,9 Mb
Release : 2019-10-31
Category : Science
ISBN : 1071601377

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Nucleic Acid Detection and Structural Investigations by Kira Astakhova,Syeda Atia Bukhari Pdf

This book volume provides in depth coverage of the nucleic acid field and aims to represent a broad diversity of the methodologies and a wide range of nucleic-acid-related topics within the fields of molecular biology and biomedicine. Chapters are divided in three sections detailing in vitro detection, nanotechnology and imaging, biomedical applications, and big data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Nucleic Acid Detection and Structural Investigations: Methods and Protocols aims present aims to present methodologies combine cutting-edge innovation with sound theory and practical applications in life sciences.

Author : Susan A. Brooks,Udo Schumacher
Publisher : Springer Science & Business Media
Page : 310 pages
File Size : 42,7 Mb
Release : 2008-02-02
Category : Medical
ISBN : 9781592591374

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Metastasis Research Protocols by Susan A. Brooks,Udo Schumacher Pdf

In Volume I, Analysis of Cells and Tissues, we presented a range of protocols aimed at mapping and analyzing the expression of various molecules of pot- tial interest in metastasis research and for examining their production at the genetic level. In this second volume of metastasis research protocols, we move to the level of living cells and tissues and present methodologies applicable to examining metastatic behavior in vitro and in whole animal models. The methods described in the first section of this volume concentrate on the separation of cell lines with high and low metastatic potential, including the genetic modification of cell lines. The assay systems to test defined aspects of the metastatic cascade are then described in Part II and include cell migration assays, assays for matrix degrading enzymes, basement membrane degrading assays, adhesion assays, and assays of angiogenesis. The role of the specific elements of the metastatic cascade assayed in each of these systems in turn must of course be put into perspective relative to their roles in entire living organisms.

Author : Bruno Latour,Steve Woolgar
Publisher : Princeton University Press
Page : 296 pages
File Size : 42,9 Mb
Release : 2013-04-04
Category : Social Science
ISBN : 9781400820412

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Laboratory Life by Bruno Latour,Steve Woolgar Pdf

This highly original work presents laboratory science in a deliberately skeptical way: as an anthropological approach to the culture of the scientist. Drawing on recent work in literary criticism, the authors study how the social world of the laboratory produces papers and other "texts,"' and how the scientific vision of reality becomes that set of statements considered, for the time being, too expensive to change. The book is based on field work done by Bruno Latour in Roger Guillemin's laboratory at the Salk Institute and provides an important link between the sociology of modern sciences and laboratory studies in the history of science.

Author : Daniel A Lafontaine,Audrey Dubé
Publisher : Humana Press
Page : 0 pages
File Size : 54,5 Mb
Release : 2013-12-07
Category : Medical
ISBN : 1627037292

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Therapeutic Applications of Ribozymes and Riboswitches by Daniel A Lafontaine,Audrey Dubé Pdf

In Therapeutic Applications of Ribozymes and Riboswitches: Methods and Protocols, expert researchers in the field provide a complete overview of protocols used in the development of RNA molecule as drugs and drug target. These include methods and protocols on recent and precise RNA molecule approaches or studies in the development of an RNA therapeutic tool. We are convinced that these methods will help researchers from various domains of life sciences, including clinicians, biochemists and virologists. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Therapeutic Applications of Ribozymes and Riboswitches: Methods and Protocols seeks to aid scientists in the further development of new therapeutic applications implicating RNA molecules or directly targeting harmful RNA.