Light Microscopic Techniques In Biology And Medicine

Author : J. James
Publisher : Springer Science & Business Media
Page : 345 pages
File Size : 54,9 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9789401014144

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Light microscopic techniques in biology and medicine by J. James Pdf

Up to about twenty-five years ago, virtually the entire field of microscopy could be overseen and even practized by any active research worker. The rapid evolution which microscopy in its broadest sense has since undergone and which has contributed greatly to our insight in many fields of biological science and medicine has, however, lead to a progressive specialisation. Both experienced investigators in clinical and biological laboratories and post graduate students, confronted with a limited number of microscopic tech niques in their daily research work, have increasing difficulty in keeping (or obtaining) a general idea of the many time-honoured and new possibilities which microscopy has to offer. This book has been written with the aim of presenting general informa tion on light microscopic techniques, at a level somewhere in between booklets like those provided by microscope manufacturers (which are often too much focussed on the production program of a particular make) and very advanced treatises with a thorough mathematical treatment of all phenomena concerned. The physically oriented texts moreover often do not sufficiently take into account the practical situation in a medical or biolog ical laboratory; on the other hand, the value of really understanding what one is doing in using a microscopic technique is often underestimated. At tempt has been made, therefore, to present sufficient background informa tion necessary for a rational application of the different microscopical tech niques in their mutual relationship.

Author : Terje Dokland,Dietmar Werner Hutmacher,Mary Mah-lee Ng,Jan-thorsten Schantz
Publisher : World Scientific Publishing Company
Page : 422 pages
File Size : 53,5 Mb
Release : 2006-09-28
Category : Technology & Engineering
ISBN : 9789813101951

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Techniques In Microscopy For Biomedical Applications by Terje Dokland,Dietmar Werner Hutmacher,Mary Mah-lee Ng,Jan-thorsten Schantz Pdf

The second volume of the series Manuals in Biomedical Research, this book is aimed to be both a concise introduction to the diverse field of microscopy and a practical guide those who require the use of microscopic for methods in their research. It provides young as well as experienced scientists a state-of-the-art multidisciplinary overview of microscopic techniques, covering all the major microscopy fields in biomedical sciences and showing their application in evaluating samples ranging from molecules to cells and tissues.Microscopy has revolutionized our understanding of biological events. Within the last two decades, microscopic techniques have provided insights into the dynamics of biological processes that regulate such events. Biological discovery, to a large extent, depends on advances in imaging techniques and various microscopic techniques have emerged as central and indispensable tools in the biomedical sciences.The four authors bring with them extensive experiences spanning across disciplines such as Microbiology, Molecular and Cell Biology, Tissue Engineering, Biomedical and Regenerative Medicine and so forth, reinforcing the fact that microscopy has proven useful in countless investigations into the mysteries of life.

Author : J. James,H.J Tanke
Publisher : Springer Science & Business Media
Page : 200 pages
File Size : 50,9 Mb
Release : 2012-12-06
Category : Medical
ISBN : 9789401137782

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Biomedical Light Microscopy by J. James,H.J Tanke Pdf

New interest in light microscopy of the last few years has not been backed up by adequate general literature. This book intends to fill the gap between specialized texts on detailed topics and general introductory booklets, mostly dealing with the use of the conventional light microscope only. In this short textbook both new developments in microscopy and basic facts of image formation will be treated, including often neglected topics such as axial resolving power, lens construction, photomicrography and correct use of phase-en interference contrast systems. Theoretical background will be dealt with as far as necessary for a well-considered application of these techniques enabling a deliberate choice for the approach of a certain problem. Over 150 illustrations (photomicrographs and diagrams) complete the information on microscopy of the nineties in the biomedical field, intended for scientists, doctors, technicians and research students. Many drawings have been contributed by the illustrator R. Kreuger; the photographic work has been executed by J. Peeterse. Secretarial assistance in preparing the manuscript was given by Ms T. M. S. Pierik. Dr M. J. Pearson has corrected the English of the final text.

Author : J.K. Koehler
Publisher : Springer Science & Business Media
Page : 319 pages
File Size : 46,6 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642654923

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Advanced Techniques in Biological Electron Microscopy by J.K. Koehler Pdf

The past decade has seen a remarkable increase in the use of electron microscopy as a researm tool in biology and medicine. Thus, most institu tions of higher learning now boast several electron optical laboratories having various levels of sophistication. Training in the routine use of elec tron optical equipment and interpretation of results is no longer restricted to a few prestigious centers. On the other hand, temniques utilized by researm workers in the ultrastructural domain have become extremely diverse and complex. Although a large number of quite excellent volumes of electron microscopic temnique are now dedicated to the basic elements available whim allow the novice to acquire a reasonable introduction to the field, relatively few books have been devoted to a discussion of more ad vanced temnical aspects of the art. It was with this view that the present volume was conceived as a handy reference for workers already having some background in the field, as an information source for those wishing to shift efforts into more promising temniques, or for use as an advanced course or seminar guide. Subject matter has been mosen particularly on the basis of pertinence to present researm activities in biological electron microscopy and emphasis has been given those areas whim seem destined to greatly expand in useful ness in the near future.

Author : Edmund Vincent Cowdry
Publisher : Unknown
Page : 220 pages
File Size : 40,6 Mb
Release : 1943
Category : Diagnosis, Laboratory
ISBN : UCAL:B4086016

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Microscopic Technique in Biology and Medicine by Edmund Vincent Cowdry Pdf

Author : Douglas E. Chandler,Robert W. Roberson
Publisher : Jones & Bartlett Publishers
Page : 468 pages
File Size : 51,8 Mb
Release : 2009
Category : Medical
ISBN : 9780763738747

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Bioimaging by Douglas E. Chandler,Robert W. Roberson Pdf

The Development Of Microscopy Revolutionized The World Of Cell And Molecular Biology As We Once Knew It And Will Continue To Play An Important Role In Future Discoveries. Bioimaging: Current Concepts In Light And Electron Microscopy Is The Optimal Text For Any Undergraduate Or Graduate Bioimaging Course, And Will Serve As An Important Reference Tool For The Research Scientist. This Unique Text Covers, In Great Depth, Both Light And Electron Microscopy, As Well As Other Structure And Imaging Techniques Like X-Ray Crystallography And Atomic Force Microscopy. Written In A User-Friendly Style And Covering A Broad Range Of Topics, Bioimaging Describes The State-Of-The-Art Technologies That Have Powered The Field To The Forefront Of Cellular And Molecular Biological Research.

Author : Michael J. Dykstra,Laura E. Reuss
Publisher : Springer Science & Business Media
Page : 537 pages
File Size : 49,9 Mb
Release : 2011-06-28
Category : Science
ISBN : 9781441992444

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Biological Electron Microscopy by Michael J. Dykstra,Laura E. Reuss Pdf

Electron microscopy is frequently portrayed as a discipline that stands alone, separated from molecular biology, light microscopy, physiology, and biochemistry, among other disciplines. It is also presented as a technically demanding discipline operating largely in the sphere of "black boxes" and governed by many absolute laws of procedure. At the introductory level, this portrayal does the discipline and the student a disservice. The instrumentation we use is complex, but ultimately understandable and, more importantly, repairable. The procedures we employ for preparing tissues and cells are not totally understood, but enough information is available to allow investigators to make reasonable choices concerning the best techniques to apply to their parti cular problems. There are countless specialized techniques in the field of electron and light microscopy that require the acquisition of specialized knowledge, particularly for interpretation of results (electron tomography and energy dispersive spectroscopy immediately come to mind), but most laboratories possessing the equipment to effect these approaches have specialists to help the casual user. The advent of computer operated electron microscopes has also broadened access to these instruments, allowing users with little technical knowledge about electron microscope design to quickly become operators. This has been a welcome advance, because earlier instru ments required a level of knowledge about electron optics and vacuum systems to produce optimal photographs and to avoid "crashing" the instruments that typically made it difficult for beginners.

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 52,6 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

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Molecular Biology of The Cell by Bruce Alberts Pdf

Author : John J. Bozzola,Lonnie Dee Russell
Publisher : Jones & Bartlett Learning
Page : 702 pages
File Size : 46,9 Mb
Release : 1999
Category : Medical
ISBN : 0763701920

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Electron Microscopy by John J. Bozzola,Lonnie Dee Russell Pdf

New edition of an introductory reference that covers all of the important aspects of electron microscopy from a biological perspective, including theory of scanning and transmission; specimen preparation; darkroom, digital imaging, and image analysis; laboratory safety; interpretation of images; and an atlas of ultrastructure. Generously illustrated with bandw line drawings and photographs. Annotation copyrighted by Book News, Inc., Portland, OR

Author : Hélio Chiarini-Garcia,Rossana Melo
Publisher : Humana Press
Page : 0 pages
File Size : 48,6 Mb
Release : 2010-12-15
Category : Science
ISBN : 1607619490

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Light Microscopy by Hélio Chiarini-Garcia,Rossana Melo Pdf

Of all scientific instruments, probably none has had more applications in the life sciences than the light microscope. In Light Microscopy: Methods and Protocols, expert researchers explore the basics and the latest advances in microscope instrumentation, sample preparation, and imaging techniques, all of which have been producing fundamental insights into the functions of cells and tissues. Chapters cover a variety of bright field and fluorescence microscopy-based approaches that are central to the study of a range of biological questions, providing information on how to prepare cells and tissues for microscopic investigations, covering detailed staining procedures, and exploring methods to analyze images and interpret the results accurately. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Comprehensive and current, Light Microscopy: Methods and Protocols is an essential handbook for all researchers who are exploring the intriguing microscopic world of the cell.

Author : David G. Robinson,Ulrich Ehlers,Rainer Herken,Bernd Herrmann,Frank Mayer,Friedrich-Wilhelm Schürmann
Publisher : Springer Science & Business Media
Page : 204 pages
File Size : 40,8 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642488481

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Methods of Preparation for Electron Microscopy by David G. Robinson,Ulrich Ehlers,Rainer Herken,Bernd Herrmann,Frank Mayer,Friedrich-Wilhelm Schürmann Pdf

In 1939, when the electron optics laboratory of Siemens & Halske Inc. began to manufacture the first electron microscopes, the biological and medical profes sions had an unexpected instrument at their disposal which exceeded the reso lution of the light microscope by more than a hundredfold. The immediate and broad application of this new tool was complicated by the overwhelming prob lems inherent in specimen preparation for the investigation of cellular struc tures. The microtechniques applied in light microscopy were no longer appli cable, since even the thinnest paraffin layers could not be penetrated by electrons. Many competent biological and medical research workers expressed their anxiety that objects in high vacuum would be modified due to complete dehydration and the absorbed electron energy would eventually cause degrada tion to rudimentary carbon backbones. It also seemed questionable as to whether it would be possible to prepare thin sections of approximately 0. 5 11m from heterogeneous biological specimens. Thus one was suddenly in posses sion of a completely unique instrument which, when compared with the light microscope, allowed a 10-100-fold higher resolution, yet a suitable preparation methodology was lacking. This sceptical attitude towards the application of electron microscopy in bi ology and medicine was supported simultaneously by the general opinion of colloid chemists, who postulated that in the submicroscopic region of living structures no stable building blocks existed which could be revealed with this apparatus.

Author : Pier Carlo Braga,Davide Ricci
Publisher : Springer Science & Business Media
Page : 388 pages
File Size : 50,7 Mb
Release : 2008-02-02
Category : Science
ISBN : 9781592596478

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Atomic Force Microscopy by Pier Carlo Braga,Davide Ricci Pdf

The natural, biological, medical, and related sciences would not be what they are today without the microscope. After the introduction of the optical microscope, a second breakthrough in morphostructural surface analysis occurred in the 1940s with the development of the scanning electron microscope (SEM), which, instead of light (i. e. , photons) and glass lenses, uses electrons and electromagnetic lenses (magnetic coils). Optical and scanning (or transmission) electron microscopes are called “far-field microscopes” because of the long distance between the sample and the point at which the image is obtained in comparison with the wavelengths of the photons or electrons involved. In this case, the image is a diffraction pattern and its resolution is wavelength limited. In 1986, a completely new type of microscopy was proposed, which, without the use of lenses, photons, or electrons, directly explores the sample surface by means of mechanical scanning, thus opening up unexpected possibilities for the morphostructural and mechanical analysis of biological specimens. These new scanning probe microscopes are based on the concept of near-field microscopy, which overcomes the problem of the limited diffraction-related resolution inherent in conventional microscopes. Located in the immediate vicinity of the sample itself (usually within a few nanometers), the probe records the intensity, rather than the interference signal, thus significantly improving resolution. Since the most we- known microscopes of this type operate using atomic forces, they are frequently referred to as atomic force microscopes (AFMs).

Author : Michael Hoppert
Publisher : John Wiley & Sons
Page : 342 pages
File Size : 45,7 Mb
Release : 2006-03-06
Category : Science
ISBN : 9783527605231

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Microscopic Techniques in Biotechnology by Michael Hoppert Pdf

Focusing on all current applications, this book presents the various methods as well as their suitability and limitations for a specific question. One particular highlight is the presentation of all basic information on the structure of the relevant objects, thus allowing readers to choose the most suitable applications for any specific problem. They will also find in-depth background information on structure-function relationships, plus descriptions of sample preparations with respect to a particular technique and the necessary equipment. The whole is rounded off with an overview of the future application potential for devices and applications of upcoming interest in biotechnology.

Author : Terence Allen
Publisher : Oxford University Press, USA
Page : 153 pages
File Size : 54,8 Mb
Release : 2015
Category : Science
ISBN : 9780198701262

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Microscopy by Terence Allen Pdf

Using light, electrons, or X-rays, microscopes today form a vital tool not only in biology but in many other disciplines, including materials science and nanotechnology. In this Very Short Introduction Terence Allen describes the scientific principles behind the main forms of microscopy, and the exciting new developments in the field. Beginning with a brief history of microscopy, Allen surveys the diverse and powerful forms of miscroscopes available today, illustrating how microscopy impinges on almost every aspect of our daily lives.

Author : Karsten König
Publisher : Walter de Gruyter GmbH & Co KG
Page : 450 pages
File Size : 44,5 Mb
Release : 2018-01-22
Category : Science
ISBN : 9783110429985

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Multiphoton Microscopy and Fluorescence Lifetime Imaging by Karsten König Pdf

This monograph demonstrates the latest developments in two-photon fluorescence microscopy and second-harmonic generation (SHG) microscopy, including coverage of high-resolution microscopy methods, such as STED microscopy. A special focus lies on clinical applications of these methods, e.g. in dermatology, ophtalmology, neuro sciences and cell biology.