Molecular Cytogenetics Methods And Protocols

Author : Camila Blair
Publisher : Unknown
Page : 246 pages
File Size : 54,8 Mb
Release : 2019-06-14
Category : Electronic
ISBN : 1632417995

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Molecular Cytogenetics: Methods and Protocols by Camila Blair Pdf

The branch of genetics, which studies how chromosomes relate to cell behavior and their behavior during mitosis and meiosis, is known as cytogenetics. Molecular cytogenetics is a field involving the combination of cytogenetics and molecular biology. It uses several reagents and focuses on identifying and distinguishing normal and cancer-causing cells. It acts as a vital tool for the treatment of malignancies like brain tumors and hematological malignancies. It includes techniques and methods, like fluorescence in situ hybridization (FISH) and comparative genomic hybridization (CGH). This book is compiled in such a manner, that it will provide in-depth knowledge about the theory and practice of molecular cytogenetics. From theories to research to practical applications, case studies related to all contemporary topics of relevance to this field have been included in it. This book aims to equip students and experts with the advanced topics and upcoming concepts in this area.

Author : Lynda J. Campbell
Publisher : Humana Press
Page : 0 pages
File Size : 49,6 Mb
Release : 2011-03-16
Category : Medical
ISBN : 1617790737

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Cancer Cytogenetics by Lynda J. Campbell Pdf

Cytogenetic studies of malignancy have become an essential tool in the clinical management of cancer patients. Cancer Cytogenetics: Methods and Protocols presents eminently practical key cytogenetic and FISH techniques for every stage of diagnostic service. Experts in the field describe detailed cytogenetic analysis methods, fluorescence in situ hybridization and array methods currently being applied to investigate and diagnose different varieties of cancer. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, and step-by-step, readily reproducible laboratory protocols. The authors of the various chapters have also provided extensive notes to guide individuals who are new to these methods through the pitfalls that bedevil all such testing. Authoritative and accessible, Cancer Cytogenetics: Methods and Protocols serves as an ideal guide to scientists of all backgrounds, allowing them to either establish new techniques in their laboratories or find the different variations of standard methods helpful in improving their results.

Author : John Swansbury
Publisher : Springer Science & Business Media
Page : 283 pages
File Size : 50,6 Mb
Release : 2008-02-05
Category : Medical
ISBN : 9781592593637

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Cancer Cytogenetics by John Swansbury Pdf

A collection of key cytogenetic and FISH techniques used by modern clinical laboratories in the genetic analysis of human malignancies. The book's practical advice and methods are suitable for use at every level of expertise, including fully established laboratories, but with a sympathetic bias towards anyone considering setting up a new cytogenetics service. Here the reader will find not only elementary tutorials on the fundamentals of human karyotypes and chromosome analysis, but also detailed discussions on how laboratories may optimally upgrade their repertoire of capabilities to include such newer complementary techniques as CGH, FISH, and M-FISH.

Author : Shahryar F. Kianian,Penny M. Avoles Kianian
Publisher : Humana
Page : 0 pages
File Size : 40,6 Mb
Release : 2016-08-10
Category : Science
ISBN : 1493936204

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Plant Cytogenetics by Shahryar F. Kianian,Penny M. Avoles Kianian Pdf

This volume covers a range of methods used in plant cytogenetics, beginning with basic analysis of chromosomes and visualizing gene locations, to manipulating and dissecting chromosomes, and then focusing on less understood features of chromosomes such as recombination initiation sites and epigenomic marks. The methods described in Plant Cytogenetics: Methods and Protocols build on each other and provide, those new to the field, with a comprehensive platform to support their research endeavours, while also introducing advanced techniques to experienced researchers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and thorough, Plant Cytogenetics: Methods and Protocols, is a valuable resource for anyone who is interested in the diverse and wonderfully complex field of cytogenetics.

Author : Yao-Shan Fan
Publisher : Humana
Page : 0 pages
File Size : 50,8 Mb
Release : 2010-11-10
Category : Medical
ISBN : 1617373001

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Molecular Cytogenetics by Yao-Shan Fan Pdf

The new techniques of molecular cytogenetics, mainly fluorescence in situ hybridization (FISH) of DNA probes to metaphase chromosomes or interphase nuclei, have been developed in the past two decades. Many FISH techniques have been implemented for diagnostic services, whereas some others are mainly used for investigational purposes. Several hundreds of FISH probes and hybridization kits are now commercially available, and the list is growing rapidly. FISH has been widely used as a powerful diagnostic tool in many areas of medicine including pediatrics, medical genetics, maternal–fetal medicine, reproductive medicine, pathology, hematology, and oncology. Frequently, a physician may be puzzled by the variety of FISH techniques and wonder what test to order. It is not uncommon that a sample is referred to a laboratory for FISH without indicating a specific test. On the other hand, a cytogeneticist or a technologist in a laboratory needs, from case to case, to determine which procedure to perform and which probe to use for an informative result. To obtain the best results, one must use the right DNA probes and have reliable protocols and measures of quality assurance in place. Also, one must have sufficient knowledge in both traditional and molecular cytogenetics, as well as the particular areas of medicine for which the test is used in order to appropriately interpret the FISH results, and to correlate them with clinical diagnosis, treatment, and prognosis.

Author : Anonim
Publisher : Unknown
Page : 128 pages
File Size : 42,6 Mb
Release : 1973
Category : Electronic
ISBN : OCLC:320473602

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Molecular Cytogenetics by Anonim Pdf

Author : Shahryar F. Kianian,Penny M. Avoles Kianian
Publisher : Humana
Page : 198 pages
File Size : 42,7 Mb
Release : 2018-06-09
Category : Science
ISBN : 1493980971

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Plant Cytogenetics by Shahryar F. Kianian,Penny M. Avoles Kianian Pdf

This volume covers a range of methods used in plant cytogenetics, beginning with basic analysis of chromosomes and visualizing gene locations, to manipulating and dissecting chromosomes, and then focusing on less understood features of chromosomes such as recombination initiation sites and epigenomic marks. The methods described in Plant Cytogenetics: Methods and Protocols build on each other and provide, those new to the field, with a comprehensive platform to support their research endeavours, while also introducing advanced techniques to experienced researchers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting edge and thorough, Plant Cytogenetics: Methods and Protocols, is a valuable resource for anyone who is interested in the diverse and wonderfully complex field of cytogenetics.

Author : Daryl S. Henderson
Publisher : Springer Science & Business Media
Page : 467 pages
File Size : 48,7 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592596652

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Drosophila Cytogenetics Protocols by Daryl S. Henderson Pdf

Leading drosophilists describe in step-by-step detail all the essential techniques for studying Drosophila chromosomes and suggest new avenues for scientific exploration. The chapters emphasize specimen preparation (from dissection to mounting) and cover both polytene and mitotic/meiotic chromosomes in depth. Each fully tested and readily reproducible protocol offers a background introduction, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls. A cutting-edge FISH and immunolocalization technique will be important for discovering how DNA sequence influences higher-order chromosome architecture and ultimately gene expression.

Author : John M. Walker
Publisher : Unknown
Page : 128 pages
File Size : 48,6 Mb
Release : 1984
Category : Molecular biology -- v.236. Plant functional genomics -- v.237. G protein signaling -- v.238. Biopolymer methods in tissue engineering -- v.239. Cell migration in inflammation and immunity -- v.240. Mammalian artificial chromosomes -- v.241. Cell cycle checkpoint control protocols -- v.242. Atomic force microscopy -- v.243. Chiral separations: Methods and protocols -- v.244. Protein purification protocols -- v.245-6. Gene delivery to mammalian cells -- v.247. Drosophila cytogenetics protocols -- v.248. Antibody engineering -- v.249. Cytokine protocols -- v.251. HPLC of peptides and proteins: Methods and protocols -- v.265. RNA interference, editing, and modification -- v.274. Photosynthesis research protocols -- v.318. Plant cell culture protocols -- v.323. Arabidopsis protocols
ISBN : LCCN:84015696

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Methods in Molecular Biology: Cancer cytogenetics: methods and protocols by John M. Walker Pdf

Author : Yao-Shan Fan
Publisher : Springer Science & Business Media
Page : 409 pages
File Size : 45,6 Mb
Release : 2008-02-05
Category : Medical
ISBN : 9781592593002

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Molecular Cytogenetics by Yao-Shan Fan Pdf

The new techniques of molecular cytogenetics, mainly fluorescence in situ hybridization (FISH) of DNA probes to metaphase chromosomes or interphase nuclei, have been developed in the past two decades. Many FISH techniques have been implemented for diagnostic services, whereas some others are mainly used for investigational purposes. Several hundreds of FISH probes and hybridization kits are now commercially available, and the list is growing rapidly. FISH has been widely used as a powerful diagnostic tool in many areas of medicine including pediatrics, medical genetics, maternal–fetal medicine, reproductive medicine, pathology, hematology, and oncology. Frequently, a physician may be puzzled by the variety of FISH techniques and wonder what test to order. It is not uncommon that a sample is referred to a laboratory for FISH without indicating a specific test. On the other hand, a cytogeneticist or a technologist in a laboratory needs, from case to case, to determine which procedure to perform and which probe to use for an informative result. To obtain the best results, one must use the right DNA probes and have reliable protocols and measures of quality assurance in place. Also, one must have sufficient knowledge in both traditional and molecular cytogenetics, as well as the particular areas of medicine for which the test is used in order to appropriately interpret the FISH results, and to correlate them with clinical diagnosis, treatment, and prognosis.

Author : Rolf-Dieter Wegner
Publisher : Springer Science & Business Media
Page : 464 pages
File Size : 54,9 Mb
Release : 2013-11-11
Category : Medical
ISBN : 9783642599187

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Diagnostic Cytogenetics by Rolf-Dieter Wegner Pdf

Following a section on tissue culture, chromosome staining and basic information about karyotyping, this text presents nomenclature and quality standards, as well as protocols of relevance to comprehensive cytogenetic diagnostics.

Author : Tony Heitkam,Sònia Garcia
Publisher : Springer Nature
Page : 563 pages
File Size : 44,9 Mb
Release : 2023-07-21
Category : Science
ISBN : 9781071632260

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Plant Cytogenetics and Cytogenomics by Tony Heitkam,Sònia Garcia Pdf

This book provides an up-to-date account of the most widespread methods used by specialists in the field of plant cytogenetics and the emerging field of cytogenomics that will likely soon be adapted by more labs. From the classical basic karyological approaches to the most recent genomics-informed and computational methods, the volume explores genome size and ploidy level estimation, chromosome fixation, preparation, and manipulation, banding and staining techniques, in situ hybridization, as well as numerous methods that integrate cytogenetics with bioinformatics and computational genomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Plant Cytogenetics and Cytogenomics: Methods and Protocols serves as an ideal resource for plant scientists interested in molecular and evolutionary biology, breeding, systematics, and plant -omics in general.

Author : Thomas Liehr
Publisher : Springer Science & Business Media
Page : 447 pages
File Size : 46,7 Mb
Release : 2008-11-26
Category : Science
ISBN : 9783540705819

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Fluorescence In Situ Hybridization (FISH) - Application Guide by Thomas Liehr Pdf

This book is a unique source of information on the present state of the exciting field of molecular cytogenetics and how it can be applied in research and diagnostics. The basic techniques of fluorescence in situ hybridization and primed in situ hybridization (PRINS) are outlined, the multiple approaches and probe sets that are now available for these techniques are described, and applications of them are presented in 36 chapters by authors from ten different countries around the world. The book not only provides the reader with basic and background knowledge on the topic, but also gives detailed protocols that show how molecular cytogenetics is currently performed by specialists in this field. The FISH Application Guide initially provides an overview of the (historical) development of molecular cytogenetics, its basic procedures, the equipment required, and probe generation. The book then describes tips and tricks for making different tissues available for molecular cytogenetic studies. These are followed by chapters on various multicolor FISH probe sets, their availability, and their pot- tial for use in combination with other approaches. The possible applications that are shown encompass the characterization of marker chromosomes, cryptic cytogenetic aberrations and epigenetic changes in humans by interphase and metaphase cyto- netics, studies of nuclear architecture, as well as the application of molecular cytogenetics to zoology, botany and microbiology.

Author : John M. Walker
Publisher : Unknown
Page : 128 pages
File Size : 42,8 Mb
Release : 1984
Category : Molecular biology -- v.236. Plant functional genomics -- v.237. G protein signaling -- v.238. Biopolymer methods in tissue engineering -- v.239. Cell migration in inflammation and immunity -- v.240. Mammalian artificial chromosomes -- v.241. Cell cycle checkpoint control protocols -- v.242. Atomic force microscopy -- v.243. Chiral separations: Methods and protocols -- v.244. Protein purification protocols -- v.245-6. Gene delivery to mammalian cells -- v.247. Drosophila cytogenetics protocols -- v.248. Antibody engineering -- v.249. Cytokine protocols -- v.251. HPLC of peptides and proteins: Methods and protocols -- v.265. RNA interference, editing, and modification -- v.274. Photosynthesis research protocols -- v.318. Plant cell culture protocols -- v.323. Arabidopsis protocols
ISBN : LCCN:84015696

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Methods in Molecular Biology: Molecular cytogenetics: protocols and applications by John M. Walker Pdf

Author : John R. Gosden
Publisher : Springer Science & Business Media
Page : 508 pages
File Size : 55,8 Mb
Release : 2008-02-02
Category : Medical
ISBN : 9781592595167

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Chromosome Analysis Protocols by John R. Gosden Pdf

Chromosomes, as the genetic vehicles, provide the basic material for a large proportion of genetic investigations, from the construction of gene maps and models of chromosome organization, to the inves tigation of gene function and dysfunction. The study of chromosomes has developed in parallel with other aspects of molecular genetics, beginning with the first preparations of chromosomes from animal cells, through the development of banding techniques, which permitted the unequivocal identification of each chromosome in a karyotype, to the present analytical methods of molecular cytogenetics. Although some of these techniques have been in use for many years, and can be learned relatively easily, most published scientific reports—as a result of pressure on space from editors, and the response to that pressure by authors—contain little in the way of technical detail, and thus are rarely adequate for a researcher hoping to find all the necessary information to embark on a method from scratch. A new user needs not only a detailed description of the methods, but also some help with problem solving and sorting out the difficulties en countered in handling any biological system. This was the require ment to which the series Methods in Molecular Biology is addressed, and Chromosome Analysis Protocols forms a part of this series.