Molecular Pathology Protocols Methods In Molecular Medicine

Author : Anthony A. Killeen
Publisher : Unknown
Page : 128 pages
File Size : 45,9 Mb
Release : 2001
Category : Electronic
ISBN : OCLC:748539234

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Molecular Pathology Protocols. Methods in Molecular Medicine by Anthony A. Killeen Pdf

Anthony Killeen has assembled a collection of readily reproducible molecular pathology techniques that are either frequently performed or recognized for their significant diagnostic utility. Each method is described in step-by-step detail by a leading molecular pathologist or laboratory scientist who has developed it or used it extensively. These clinical laboratory techniques can be used for the diagnosis or monitoring of cancer, hematological malignancies, infectious diseases, and selected genetic disorders. Comprehensive and path-breaking, Molecular Pathology Protocols will enable clinical laboratories to introduce new molecular pathology tests and lay the groundwork for a much-needed standardization in this rapidly developing field.

Author : Anthony A. Killeen
Publisher : Springer Science & Business Media
Page : 488 pages
File Size : 54,6 Mb
Release : 2008-02-02
Category : Medical
ISBN : 9781592590810

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Molecular Pathology Protocols by Anthony A. Killeen Pdf

The era of molecular pathology has arrived. From its promising beg- nings in research laboratories, the field has grown, and continues to grow, to become a vital part of the care of an ever-increasing number of patients. Because of its recent emergence from the research taboratory, many molecuIar pathology protocols we still to be found in the primary litcramre, and have not appeared in a text. MO~PCU~Q~ Padhoiogy Protocob contains la- ratory protocoIs that have been developed by many of the authors for use in clinical molecular pathology laboratories and describe in detail Row to perform these assays. This book is therefore intended for clinical laboratory use by medical technologists and pathologists. It will dso be of interest to research workers who are performing these assays. In its broadest meaning, pathology is the study of disease, and therefore it follows that any disease for which the molecular basis is understood would be suitable as a topic for inclusion in this work. When seiecting protocols, it was necessary to place limits on the number of chapters that could be feasibly presented in a single work. Those protoculs that were selected are performed more frequently, or have achieved recognition as having important diagnostic utility in contemporary practice. A decision was made to exclude inherited genetic diseases with certain exceptions, such as those diseases that are associated with thrombotic states and are part of the traditional dumain of pathology.

Author : Virginia Espina,Lance Liotta
Publisher : Humana Press
Page : 0 pages
File Size : 41,8 Mb
Release : 2011-11-12
Category : Science
ISBN : 1603272151

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Molecular Profiling by Virginia Espina,Lance Liotta Pdf

The next revolution in molecular medicine is the application of molecular profiling to individualized patient therapy. Molecular profiling technology has advanced dramatically, particularly in the field of cancer tissue biomarkers. It is now possible to gather complex genomic and proteomic information from a routine clinical needle biopsy or surgical specimen. In Molecular Profiling : Methods and Protocols, expert researchers in the field focus on the entire process from discovery to commercialization, with practical guides that are not limited to experimental methods. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as essays and guidelines for grants, patents, and commercialization of products related to molecular profiling. Authoritative and practical, Molecular Profiling: Methods and Protocols seeks to aid scientists in understanding the latest advancements in genomics, proteomics, imagining, and bioinformatics.

Author : Peter Hu,Madhuri Hegde,Patrick Alan Lennon
Publisher : Springer Science & Business Media
Page : 428 pages
File Size : 45,5 Mb
Release : 2012-05-11
Category : Medical
ISBN : 9781461421702

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Modern Clinical Molecular Techniques by Peter Hu,Madhuri Hegde,Patrick Alan Lennon Pdf

This timely book covers the need to know clinical practices for all those involved in molecular laboratory science. The field of molecular medicine is evolving at an astounding speed. Propelled by the new insights and technologies, advances are being made at an unprecedented rate. With dual measure given to today’s breakthroughs, this book is a collection of the most current practices relevant to the clinical molecular laboratorian. It begins with an introductory section on techniques and procedure. It then presents four separate sections on infectious disease, oncology, pre/post-natal, and identity testing, with specific chapters clearly outlining clinical protocols used in daily practice. Modern Clinical Molecular Techniques cuts to the heart of what is essential for the practicing molecular laboratory scientist. It is an outstanding resource for those operating within or looking to set up a clinical molecular laboratory.

Author : Joseph E. Roulston,John M. S. Bartlett
Publisher : Springer Science & Business Media
Page : 389 pages
File Size : 40,9 Mb
Release : 2008-02-01
Category : Medical
ISBN : 9781592597604

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Molecular Diagnosis of Cancer by Joseph E. Roulston,John M. S. Bartlett Pdf

We are currently experiencing a fundamental shift in the way in which we approach the characterization of cancer. Never before has the make up of cancer tissues and individual cells been so exhaustively researched and char- terized. We are now capable of producing molecular “fingerprints” that ch- acterize the expression of all known and unknown genes within tumors and their surrounding tissues. More than 30,000 different genes may be measured in each patient’s tumor in a single experiment. Simultaneously, novel therapies that exploit the molecular roadmap have been developed and are now being offered to patients. These novel agents, such as Glivec, Herceptin, Iressa, and others, specifically target individual genes within tumors and can produce d- matic responses in some patients. These drugs are only the forerunners of a coming tidal wave of novel therapeutics that individually target specific m- ecules within cancer cells—more than 300 such agents are currently in phase I or II clinical trials. This is an exciting time for cancer specialists and patients alike. However, if we have learned anything from the past 50 or more years of research into cancer, it is that Lord Beaverbrook, in founding the British national health service in the 1950s, was frighteningly prescient when he defined the primary goal of health care to be “Diagnosis, Diagnosis, Diag- sis. ” Now, more than ever, it is essential that appropriate diagnostic methods and approaches are applied to the selection of patients for treatment.

Author : Giorgio Stanta
Publisher : Springer Science & Business Media
Page : 301 pages
File Size : 47,9 Mb
Release : 2011-06-06
Category : Medical
ISBN : 9783642178900

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Guidelines for Molecular Analysis in Archive Tissues by Giorgio Stanta Pdf

A huge amount of fixed and paraffin-embedded tissue is stored in every hospital. This is very precious material that can be used for translational research and for diagnostics. The molecular methods employed for analysis of these tissues are similar to the usual molecular biology and proteomics methods, but reliable results can be obtained only if specific steps are followed with great care. This book provides detailed and precise guidelines for molecular analysis of archive tissues and will serve as an invaluable aid for researchers and pathologists involved in translational research and diagnostics. Clear notes and explanations are included to simplify use of the protocols for the less experienced. The authors are a group of acknowledged experts who have developed the described methods and validated them within the European project "Archive Tissues: Improving Molecular Medicine Research and Clinical Practice - IMPACTS", which has involved 21 leading institutions in 11 countries.

Author : Christina E. Day
Publisher : Unknown
Page : 416 pages
File Size : 48,7 Mb
Release : 2014
Category : Biomedicine
ISBN : 1493910507

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Histopathology by Christina E. Day Pdf

Histopathology: Methods and Protocols provides a comprehensive guide to the current issues in histopathology. With chapters on organ-based approaches with specific protocols for morphologic, molecular examination, and pathological observations governing the therapeutic management of the diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Histopathology: Methods and Protocols seeks to be a useful reference for pathologists, pathology residents, and fellows as well as to the clinicians and scientists.

Author : Peter Hu,Madhuri Hegde,Patrick Alan Lennon
Publisher : Springer
Page : 436 pages
File Size : 52,8 Mb
Release : 2016-08-23
Category : Medical
ISBN : 1493941488

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Modern Clinical Molecular Techniques by Peter Hu,Madhuri Hegde,Patrick Alan Lennon Pdf

This timely book covers the need to know clinical practices for all those involved in molecular laboratory science. The field of molecular medicine is evolving at an astounding speed. Propelled by the new insights and technologies, advances are being made at an unprecedented rate. With dual measure given to today’s breakthroughs, this book is a collection of the most current practices relevant to the clinical molecular laboratorian. It begins with an introductory section on techniques and procedure. It then presents four separate sections on infectious disease, oncology, pre/post-natal, and identity testing, with specific chapters clearly outlining clinical protocols used in daily practice. Modern Clinical Molecular Techniques cuts to the heart of what is essential for the practicing molecular laboratory scientist. It is an outstanding resource for those operating within or looking to set up a clinical molecular laboratory.

Author : John M. S. Bartlett
Publisher : Unknown
Page : 407 pages
File Size : 51,8 Mb
Release : 2004
Category : Cancer
ISBN : OCLC:990662164

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Molecular Diagnosis of Cancer by John M. S. Bartlett Pdf

Author : Gregory A. Denomme,Maria Rios,Marion E. Reid
Publisher : Elsevier
Page : 197 pages
File Size : 47,6 Mb
Release : 2000-04-27
Category : Medical
ISBN : 9780080536903

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Molecular Protocols in Transfusion Medicine by Gregory A. Denomme,Maria Rios,Marion E. Reid Pdf

This essential methods manual for immunohematologists (or hematologists and immunohematologists) provides information on genes that encode antigens on red blood cells, platelets and neutrophils. The book begins by covering general concepts in molecular biology and specific protocols such as DNA preparation, PCR-RFLP and allele-specific PCR. Information on the erythrocyte, platelet and neutrophil antigen systems and the molecular basis of polymorphisms are presented clearly in a gene facts sheet format. Database accession numbers and useful adjuncts such as Request forms, worksheets for PCR/enzyme digests also serve to benefit the user. The information is clearly presented and easily accessible and is complemented by the excellent diagrams and tabular material. This book is invaluable for both new and experienced researchers in the field and other related disciplines. Essential for hematologists and those involoved in tissue typing and the study of human genetic polymorphisms Presents clearly and concisely the information on a particular variant and the technique used to detect it Organized by antigen and provides sequences of polymorphisms and primers Details the general concepts and critical information on genes, their products, and sources of relevant nucleic acids Includes protocols that allow investigators to set up assays with minimal effort (protocols include primers, reagents, reaction conditions, sizes of amplified products, restriction fragment digests, and the relevant safety information) Provides information that helps interpret results in clinical settings Contains additional sources of information (e.g., key references, web site addresses, glossary, Database accession numbers, request forms, and worksheets for PCR/enzyme digests)

Author : Laura J. Tafe,Maria E. Arcila
Publisher : Springer Nature
Page : 325 pages
File Size : 45,8 Mb
Release : 2019-09-26
Category : Medical
ISBN : 9783030229221

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Genomic Medicine by Laura J. Tafe,Maria E. Arcila Pdf

The field of Molecular Diagnostics is rapidly evolving and molecular characterization of neoplasms is becoming an increasingly important part of the pathologic work up and diagnosis of many tumor types. This work provides a high-yield reference book that compiles critical information related to molecular biomarkers for various solid tumor and hematologic malignancy subtypes. It is succinct yet comprehensive enough to be suitable for fellows in training and medical professionals with an interest in molecular pathology and biomarkers. The book covers many aspects of molecular diagnostics, from techniques to applications and comprehensive summaries of the current molecular biomarkers of critical importance in solid and liquid tumors. Attention is also specifi cally devoted to bioinformatics and next generation sequencing, as well as pre-analytical issues that must be considered for accurate interpretation of molecular results in the context of overall patient care. This text focuses on clinical utility and validity and serves as an “owner’s manual” in Genomic Diagnostics for the practicing pathologist, pathology fellows and residents and other health care providers. Physicians will find this book invaluable as a quick reference for current molecular testing modalities and guidelines, tumor board preparation, deciding which test to order and interpreting genomic laboratory results. In addition, it is an accessible for trainees as a board review preparation reference.

Author : Constance Oliver,Maria Célia Jamur
Publisher : Humana Press
Page : 416 pages
File Size : 44,9 Mb
Release : 2012-04-06
Category : Medical
ISBN : 1617796824

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Immunocytochemical Methods and Protocols by Constance Oliver,Maria Célia Jamur Pdf

Antibodies tagged with fuorescent markers have been used in histochemistry for over 50 years. Although early applications were focused on the detection of microbial antigens in tissues, the use of immunocytochemical methods now has spread to include the det- tion of a wide array of antigens including proteins, carbohydrates, and lipids from virtually any organism. Today, immunohistochemistry is widely used to identify, in situ, various components of cells and tissues in both normal and pathological conditions. The method gains its strength from the extremely sensitive interaction of a specifc antibody with its antigen. For some scientifc areas, books have been published on applications of immu- cytochemical techniques specifc to that area. What distinguished Immunocytochemical Methods and Protocols from earlier books when it was frst published was its broad appeal to investigators across all disciplines, including those in both research and clinical settings. The methods and protocols p- sented in the frst edition were designed to be general in their application; the accompa- ing “Notes” provided the reader with invaluable assistance in adapting or troubleshooting the protocols. These strengths continued to hold true for the second edition and again for the third edition. Since the publication of the frst edition, the application of immuno- tochemical techniques in the clinical laboratory has continued to rise and this third edition provides methods that are applicable to basic research as well as to the clinical laboratory.

Author : Josie Ursini-Siegel,Nicole Beauchemin
Publisher : Humana
Page : 0 pages
File Size : 45,8 Mb
Release : 2016-09-01
Category : Medical
ISBN : 1493937995

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The Tumor Microenvironment by Josie Ursini-Siegel,Nicole Beauchemin Pdf

This book covers core and emerging in vitro and in vivo protocols used to study how various components of the tumor microenvironment are established and subsequently interact with tumor cells to facilitate carcinogenesis. In addition, the book examines research topics including cellular and molecular biology approaches, in vivo genetic approaches, various “omics”-based strategies, therapeutic strategies to target the microenvironment, and, finally, advanced techniques in the fields of tissue engineering and nanotechnology. Written and validated in the laboratories of a number of trusted collaborating authors for the highly successful Methods in Molecular Biology series, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, The Tumor Microenvironment: Methods and Protocols constitutes a compendium of techniques now available to a broad audience, including basic and clinician scientists, systems biologists, and biological engineers.

Author : Wayne W. Grody,Robert M. Nakamura,Frederick L. Kiechle,Charles Strom
Publisher : Academic Press
Page : 736 pages
File Size : 50,6 Mb
Release : 2009-11-06
Category : Science
ISBN : 0080919049

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Molecular Diagnostics by Wayne W. Grody,Robert M. Nakamura,Frederick L. Kiechle,Charles Strom Pdf

Advances in genomic and proteomic profiling of disease have transformed the field of molecular diagnostics, thus leading the way for a major revolution in clinical practice. While the range of tests for disease detection and staging is rapidly expanding, many physicians lack the knowledge required to determine which tests to order and how to interpret results. Molecular Diagnostics provides a complete guide to the use and interpretation of molecular testing in the clinical arena. No other available resource offers this emphasis, comprehensive scope, and practical utility in the clinical setting. Serves as the definitivereference for molecular pathologists worldwide Covers a variety of molecular techniques including next generation sequencing, tumor somatic cell genotyping, infectious and genetic disease tecting, and pharmacogenetics Discusses in the detail issues concerning quality assurance, regulation, ethics, and future directions for the science

Author : Barbara Driscoll
Publisher : Springer Science & Business Media
Page : 589 pages
File Size : 55,9 Mb
Release : 2008-01-18
Category : Medical
ISBN : 9781592593231

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Lung Cancer by Barbara Driscoll Pdf

The Methods in Molecular Medicine series is intended as a resource for both novice and experienced investigators attempting to diversify their tech- logical base in research. Lung Cancer: Volume 1: Molecular Pathology Me- ods and Reviews presents an overview of the current status of assays employed to detect and characterize the multitude of pathologies that contribute to the development of this deadly disease. As with all volumes in the Methods in Molecular Medicine series, the reader should find that each methods-based chapter provides clear instructions for the performance of various protocols, supplemented by additional technical notes that provide valuable insight. These notes are designed to enable the reader to acquire the techniques described with a proficiency not easily achieved by re- ing standard method formats. No volume can exhaustively cover every aspect of biological research and there will be gaps in this endeavor that one or another research group will id- tify. Each section herein could readily be expanded into a book in its own right. However, I have sought to include a spectrum of techniques that should allow for the acquisition of key skills in each area covered.