Multi Parametric Live Cell Microscopy Of 3d Tissue Models

Author : Ruslan I. Dmitriev
Publisher : Springer
Page : 172 pages
File Size : 40,5 Mb
Release : 2017-10-26
Category : Science
ISBN : 9783319673585

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Multi-Parametric Live Cell Microscopy of 3D Tissue Models by Ruslan I. Dmitriev Pdf

This book provides an essential overview of existing state-of-the-art quantitative imaging methodologies and protocols (intensity-based ratiometric and FLIM/ PLIM). A variety of applications are covered, including multi-parametric quantitative imaging in intestinal organoid culture, autofluorescence imaging in cancer and stem cell biology, Ca2+ imaging in neural ex vivo tissue models, as well as multi-parametric imaging of pH and viscosity in cancer biology. The current state-of-the-art of 3D tissue models and their compatibility with live cell imaging is also covered. This is an ideal book for specialists working in tissue engineering and designing novel biomaterial.

Author : Nirmal Mazumder,Gireesh Gangadharan,Yury V. Kistenev
Publisher : Springer Nature
Page : 265 pages
File Size : 42,8 Mb
Release : 2022-05-19
Category : Medical
ISBN : 9789811913525

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Advances in Brain Imaging Techniques by Nirmal Mazumder,Gireesh Gangadharan,Yury V. Kistenev Pdf

The book reviews the recent developments in brain imaging and their technological advancements to understand molecular mechanisms associated with neurological disorders and basic behaviors in humans and rodents at the structural, molecular, and functional levels. It discusses the usefulness of advanced optical microscopy techniques, including optical coherence tomography (OCT), miniscope, multiphoton fluorescence (2PF & 3PF), adaptive optics, harmonic generation, and Raman microscopy for understanding pathomechanism of brain disorders and pathological and physiological changes associated with neurodegenerative diseases. Also, the book presents conventional imaging modalities, including Magnetic Resonance Imaging (MRI), for delineating underlying mechanisms and precise early diagnosis of neurological disorders. This book is a useful resource for neuroscientists and researchers working in biomedical engineering and optics.

Author : Paloma Ordóñez-Morán
Publisher : Springer Nature
Page : 273 pages
File Size : 55,6 Mb
Release : 2023-06-13
Category : Science
ISBN : 9781071630761

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Intestinal Differentiated Cells by Paloma Ordóñez-Morán Pdf

This volume details the most up-to-date methods of intestinal differentiation program. Chapters guide readers through techniques to characterize intestinal differentiated cell, RNAseq, single-cell RNAseq transcriptional profiling methods, isolation of intestinal crypts, and methods for studying intestinal differentiated cancer cells. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Intestinal Differentiated Cells: Methods and Protocols aims to be comprehensive guide for researchers.

Author : Dmitri B Papkovsky,Ruslan I Dmitriev
Publisher : Royal Society of Chemistry
Page : 384 pages
File Size : 40,8 Mb
Release : 2018-05-25
Category : Science
ISBN : 9781788014557

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Quenched-phosphorescence Detection of Molecular Oxygen by Dmitri B Papkovsky,Ruslan I Dmitriev Pdf

Significant progress has been made in recent years in quenched-phosphorescence oxygen sensing, particularly in the materials and applications of this detection technology that are open to commercialization, like uses in brain imaging and food packaging. Prompted by this, the editors have delivered a dedicated book that brings together these developments, provides a comprehensive overview of the different detection methodologies, and representative examples and applications. This book is intended to attract new researchers from various disciplines such as chemistry, physics, biology and medicine, stimulate further progress in the field and assist in developing new applications. Providing a concise summary at the cutting edge, this practical guide for current experts and new potential users will increase awareness of this versatile sensing technology.

Author : Anonim
Publisher : Frontiers Media SA
Page : 156 pages
File Size : 48,9 Mb
Release : 2021-09-01
Category : Science
ISBN : 9782889712526

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Modern Tools for Time-Resolved Luminescence Biosensing and Imaging by Anonim Pdf

Author : Karen J.L. Burg,Didier Dréau,Timothy Burg
Publisher : CRC Press
Page : 638 pages
File Size : 45,8 Mb
Release : 2017-07-28
Category : Medical
ISBN : 9781351644549

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Engineering 3D Tissue Test Systems by Karen J.L. Burg,Didier Dréau,Timothy Burg Pdf

Engineering 3D Tissue Test Systems provides an introduction to, and unique coverage of, a rapidly evolving area in biomaterials engineering. It reveals the current and future research responses, the current and future diagnostic applications, and provides a comprehensive overview to foster innovation. It offers insight into the importance of 3D systems and their use as benchtop models, spanning applications from basic scientific research to clinical diagnostics. Methods and limitations of building 3D tissue structures are evaluated, with attention given to the cellular, polymeric, and fabrication instrumentation components. The book covers the important aspects of polymeric tissue test systems, highlighting the needs and constraints of the industry, and includes a chapter on regulatory and pricing issues.

Author : Wolfgang Becker
Publisher : Springer
Page : 624 pages
File Size : 42,9 Mb
Release : 2015-04-13
Category : Science
ISBN : 9783319149295

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Advanced Time-Correlated Single Photon Counting Applications by Wolfgang Becker Pdf

This book is an attempt to bridge the gap between the instrumental principles of multi-dimensional time-correlated single photon counting (TCSPC) and typical applications of the technique. Written by an originator of the technique and by sucessful users, it covers the basic principles of the technique, its interaction with optical imaging methods and its application to a wide range of experimental tasks in life sciences and clinical research. The book is recommended for all users of time-resolved detection techniques in biology, bio-chemistry, spectroscopy of live systems, live cell microscopy, clinical imaging, spectroscopy of single molecules, and other applications that require the detection of low-level light signals at single-photon sensitivity and picosecond time resolution.

Author : Kaoru Yamanouchi,Sergey Tunik,Vladimir Makarov
Publisher : Springer
Page : 518 pages
File Size : 53,9 Mb
Release : 2019-01-23
Category : Science
ISBN : 9783030059743

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Progress in Photon Science by Kaoru Yamanouchi,Sergey Tunik,Vladimir Makarov Pdf

This second volume of “Progress in Photon Science – Recent Advances” presents the latest achievements made by world-leading researchers in Russia and Japan. Thanks to recent advances in light source technologies; detection techniques for photons, electrons, and charged particles; and imaging technologies, the frontiers of photon science are now being expanding rapidly. Readers will be introduced to the latest research efforts in this rapidly growing research field through topics covering bioimaging and biological photochemistry, atomic and molecular phenomena in laser fields, laser–plasma interaction, advanced spectroscopy, electron scattering in laser fields, photochemistry on novel materials, solid-state spectroscopy, photoexcitation dynamics of nanostructures and clusters, and light propagation.

Author : Giulia Adriani,Paola Cappello,Sara Lovisa
Publisher : Frontiers Media SA
Page : 171 pages
File Size : 44,6 Mb
Release : 2023-10-26
Category : Medical
ISBN : 9782832537039

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Preclinical Models and Emerging Technologies to Study the Effects of the Tumor Microenvironment on Cancer Heterogeneity and Drug Resistance by Giulia Adriani,Paola Cappello,Sara Lovisa Pdf

Author : Xunbin Wei,Bobo Gu
Publisher : Springer Nature
Page : 309 pages
File Size : 55,6 Mb
Release : 2021-05-29
Category : Medical
ISBN : 9789811576270

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Optical Imaging in Human Disease and Biological Research by Xunbin Wei,Bobo Gu Pdf

The book introduces readers to the basic principle of optical imaging technologies. Focusing on human disease diagnostics using optical imaging methods, it provides essential information for researchers in various fields and discusses the latest trends in optical imaging. In recent decades, there has been a huge increase in imaging technologies and their applications in human diseases diagnostics, including magnetic resonance imaging, x-ray computed tomography, and nuclear tomographic imaging. This book promotes further developments to extend optical imaging to a wider range of disease diagnostics. It is a valuable resource for researchers and students in the field of biomedical optics, as well as for clinicians.

Author : Susie Nilsson
Publisher : Academic Press
Page : 218 pages
File Size : 43,7 Mb
Release : 2022-07-12
Category : Science
ISBN : 9780323910927

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Recapitulating the Stem Cell Niche ex Vivo by Susie Nilsson Pdf

Recapitulating The Stem Cell Niche Ex Vivo, Volume Six in the Advances in Stem Cells and their Niches series, highlights new advances in the field, with this new volume presenting interesting chapters on a variety of topics, including Recapitulating the bone marrow stem cell niche ex vivo, The generation of the liver ex vivo, Recapitulating the thymic stem cell niche ex vivo, Recapitulating the intestinal epithelium stem cell niche ex vivo, Recapitulating the lung stem cell niche in vitro, Recapitulating mammary tissue in vitro, and Recapitulating muscle in vitro. Provides the authority and expertise of leading contributors from an international board of authors Presents the latest release in the Advances in Stem Cells and their Niches series Includes the latest information on Recapitulating the stem cell niche ex vivo

Author : Desmond O'Connor
Publisher : Academic Press
Page : 298 pages
File Size : 40,6 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323141444

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Time-correlated single photon counting by Desmond O'Connor Pdf

Time-correlated Single Photon Counting has been written in the hope that by relating the authors' experiences with a variety of different single photon counting systems, they may provide a useful service to users and potential users of this formidably sensitive technique. Of all the techniques available to obtain information on the rates of depopulation of excited electronic singlet states of molecular species, monitoring of fluorescence provides, in principle, the simplest and most direct measure of concentration. This volume comprises eight chapters, with the first focusing on the time dependence and applications of fluorescence. Succeeding chapters go on to discuss basic principles of the single photon counting lifetime measurement; light sources; photomultipliers; electronics; data analysis; nanosecond time-resolved emission spectroscopy; time dependence of fluorescence anisotropy. This book will be of interest to practitioners in the field of chemistry.

Author : Xiangsheng Zuo,Ying Shen,Yi Yao,Jiayan Chen
Publisher : Frontiers Media SA
Page : 170 pages
File Size : 52,9 Mb
Release : 2024-03-18
Category : Science
ISBN : 9782832546284

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New Advancement in Tumor Microenvironment Remodeling and Cancer Therapy by Xiangsheng Zuo,Ying Shen,Yi Yao,Jiayan Chen Pdf

Tumor microenvironment (TME) refers to the area with non-malignant cells, signaling molecules and blood vessels that surrounds and feeds tumor cells. A tumor can change its microenvironment, and TME is also known to profoundly impact the occurrence, growth, metastasis and therapeutic response and resistance of tumors. The special paracrine characteristics, microvascular system, extracellular matrix components and stromal cells in TME promote local tumor immune suppression and could alter the penetration and distribution of drugs into tumor tissues. Accumulating evidence demonstrates that currently available tumor therapies (i.e., chemotherapy, radiotherapy, anti-vascular therapy, small molecule targeted therapy, immune checkpoint inhibitors) also in turn greatly affect the structure, function, and physical and chemical properties of TME, leading to TME remodeling. However, whether and how interaction of tumor cells and TME modeling can be dissected and targeted to further understand the underlying molecular mechanisms and establish effective new tumor therapies remains elusive.

Author : Gabriela Kania,Przemyslaw Blyszczuk,Kenneth Boheler
Publisher : Frontiers Media SA
Page : 103 pages
File Size : 47,9 Mb
Release : 2021-02-02
Category : Medical
ISBN : 9782889664443

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Cutting Edge Methodologies in Experimental Cardiovascular Research by Gabriela Kania,Przemyslaw Blyszczuk,Kenneth Boheler Pdf

Author : Christine Mummery,Anja van de Stolpe,Bernard Roelen,Hans Clevers
Publisher : Academic Press
Page : 448 pages
File Size : 50,9 Mb
Release : 2014-05-23
Category : Science
ISBN : 9780124115675

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Stem Cells by Christine Mummery,Anja van de Stolpe,Bernard Roelen,Hans Clevers Pdf

The second edition of Stem Cells: Scientific Facts and Fiction provides the non-stem cell expert with an understandable review of the history, current state of affairs, and facts and fiction of the promises of stem cells. Building on success of its award-winning preceding edition, the second edition features new chapters on embryonic and iPS cells and stem cells in veterinary science and medicine. It contains major revisions on cancer stem cells to include new culture models, additional interviews with leaders in progenitor cells, engineered eye tissue, and xeno organs from stem cells, as well as new information on "organs on chips" and adult progenitor cells. In the past decades our understanding of stem cell biology has increased tremendously. Many types of stem cells have been discovered in tissues that everyone presumed were unable to regenerate in adults, the heart and the brain in particular. There is vast interest in stem cells from biologists and clinicians who see the potential for regenerative medicine and future treatments for chronic diseases like Parkinson's, diabetes, and spinal cord lesions, based on the use of stem cells; and from entrepreneurs in biotechnology who expect new commercial applications ranging from drug discovery to transplantation therapies. Explains in straightforward, non-specialist language the basic biology of stem cells and their applications in modern medicine and future therapy Includes extensive coverage of adult and embryonic stem cells both historically and in contemporary practice Richly illustrated to assist in understanding how research is done and the current hurdles to clinical practice