Next Generation Sequencing Ngs Technology In Dna Analysis

Author : Hirak Ranjan Dash,Kelly M. Elkins,Noora Rashid Al-Snan
Publisher : Elsevier
Page : 612 pages
File Size : 54,9 Mb
Release : 2023-12
Category : Science
ISBN : 9780323991445

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Next Generation Sequencing (NGS) Technology in DNA Analysis by Hirak Ranjan Dash,Kelly M. Elkins,Noora Rashid Al-Snan Pdf

Next Generation Sequencing (NGS) Technology in DNA Analysis explains and summarizes next generation sequencing (NGS) technological applications in the field of forensic DNA analysis. The book covers the transition from capillary electrophoresis (CE)-based technique to NGS platforms and the fundamentals of NGS technologies, applications, and advances. Sections provide an overview of NGS technology and forensic science, including information on processing biological samples for forensic analysis, sequence analysis, and data analysis software as well as the analysis of NGS data. The book explores the valuable applications of NGS-based forensic DNA analysis and covers the validations and interpretation guidelines of NGS workflows. With chapter contributions from an international array of experts and the inclusion of practical case studies, this book is a useful reference for academicians and researchers in genetics, biotechnology, bioinformatics, biology, and medicine as well as forensic DNA scientists and practitioners who aim to learn, use, apply, and validate NGS-based technologies.

Author : Jerzy Kulski
Publisher : BoD – Books on Demand
Page : 466 pages
File Size : 45,9 Mb
Release : 2016-01-14
Category : Medical
ISBN : 9789535122401

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Next Generation Sequencing by Jerzy Kulski Pdf

Next generation sequencing (NGS) has surpassed the traditional Sanger sequencing method to become the main choice for large-scale, genome-wide sequencing studies with ultra-high-throughput production and a huge reduction in costs. The NGS technologies have had enormous impact on the studies of structural and functional genomics in all the life sciences. In this book, Next Generation Sequencing Advances, Applications and Challenges, the sixteen chapters written by experts cover various aspects of NGS including genomics, transcriptomics and methylomics, the sequencing platforms, and the bioinformatics challenges in processing and analysing huge amounts of sequencing data. Following an overview of the evolution of NGS in the brave new world of omics, the book examines the advances and challenges of NGS applications in basic and applied research on microorganisms, agricultural plants and humans. This book is of value to all who are interested in DNA sequencing and bioinformatics across all fields of the life sciences.

Author : Kelly M. Elkins,Cynthia B. Zeller
Publisher : CRC Press
Page : 176 pages
File Size : 50,5 Mb
Release : 2021-09-15
Category : Science
ISBN : 9781000449259

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Next Generation Sequencing in Forensic Science by Kelly M. Elkins,Cynthia B. Zeller Pdf

Next Generation Sequencing in Forensic Science: A Primer addresses next generation sequencing (NGS) specific to its application to forensic science. The first part of the book offers a history of human identity approaches, including VNTR, RFLP, STR, and SNP DNA typing. It discusses the history of sequencing for human DNA typing, including Sanger sequencing, SNaPshot, pyrosequencing, and principles of next generation sequencing. The chapters present an overview of the forensically focused AmpliSeq, ForenSeq, Precision ID, PowerSeq, and QIAseq panels for human DNA typing using autosomal, Y and X chromosome STRs and SNPs using the MiSeq FGx and Ion Torrent System. The authors outline the steps included in DNA extraction and DNA quantitation that are performed prior to preparing libraries with the NGS kits. The second half of the book details the implementation of ForenSeq and Precision ID to amplify and tag targets to create the library, enrich targets to attach indexes and adaptors, perform library purification and normalization, pool the libraries, and load samples to the cartridge to perform the sequencing on the instrument. Coverage addresses the operation of the MiSeq FGx and Ion Chef, including creating a sample list, executing wash steps, performing NGS, understanding the run feedback files from the instrument, and troubleshooting. ForenSeq and Precision ID panel data analysis are explained, including how to analyze and interpret NGS data and output graphs and charts. The book concludes with mitochondrial DNA (mtDNA) sequencing and SNPs analysis, including the issue of heteroplasmy. The final chapters review forensic applications of microbial DNA, NGS in body fluid analysis, and challenges and considerations for future applications. FEATURES Focuses on human identification using traditional and NGS DNA typing methods targeting short tandem repeats (STRs) Applies the technology and its application to law enforcement investigations and identity and ancestry single nucleotide polymorphisms (SNPs) for investigational leads, mass disaster, and ancestry cases Presents the underlying principles of NGS in a clear, easy-to-understand format for practitioners and students studying DNA in forensic programs This is the first book to prepare practitioners to utilize and implement this new technology in their lab for casework, highlighting early applications of how NGS results have been used in court. The book can be utilized for upper-level undergraduate and graduate students taking courses focused on NGS concepts. Readers are expected to have a basic understanding of molecular and cellular biology and DNA typing.

Author : C. Alexander Valencia,M. Ali Pervaiz,Ammar Husami,Yaping Qian,Kejian Zhang
Publisher : Springer Science & Business Media
Page : 94 pages
File Size : 55,9 Mb
Release : 2013-10-16
Category : Medical
ISBN : 9781461490326

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Next Generation Sequencing Technologies in Medical Genetics by C. Alexander Valencia,M. Ali Pervaiz,Ammar Husami,Yaping Qian,Kejian Zhang Pdf

This book introduces readers to Next Generation Sequencing applications in medical genetics. The authors discuss the direct application of next-generation sequencing to medicine, specifically, laboratory medicine or molecular diagnostics. The first part of the book contains chapters on sanger sequencing, NGS technologies, targeted-amplification and capture, and exome sequencing. The second part of the book focuses on genetic disorders diagnoses by NGS, prenatal diagnosis, muscular dystrophies, mitochondrial disorders diagnosis, and challenges in molecular diagnosis. Recent developments and potential future trends in NGS sequencing applications are highlighted, as well.​

Author : Kuo Ping Chiu
Publisher : Bentham Science Publishers
Page : 160 pages
File Size : 55,9 Mb
Release : 2015-11-04
Category : Science
ISBN : 9781681080925

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Next-Generation Sequencing and Sequence Data Analysis by Kuo Ping Chiu Pdf

Nucleic acid sequencing techniques have enabled researchers to determine the exact order of base pairs - and by extension, the information present - in the genome of living organisms. Consequently, our understanding of this information and its link to genetic expression at molecular and cellular levels has lead to rapid advances in biology, genetics, biotechnology and medicine. Next-Generation Sequencing and Sequence Data Analysis is a brief primer on DNA sequencing techniques and methods used to analyze sequence data. Readers will learn about recent concepts and methods in genomics such as sequence library preparation, cluster generation for PCR technologies, PED sequencing, genome assembly, exome sequencing, transcriptomics and more. This book serves as a textbook for students undertaking courses in bioinformatics and laboratory methods in applied biology. General readers interested in learning about DNA sequencing techniques may also benefit from the simple format of information presented in the book.

Author : Lee-Jun C. Wong
Publisher : Springer Science & Business Media
Page : 302 pages
File Size : 54,8 Mb
Release : 2013-05-31
Category : Medical
ISBN : 9781461470014

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Next Generation Sequencing by Lee-Jun C. Wong Pdf

In recent years, owing to the fast development of a variety of sequencing technologies in the post human genome project era, sequencing analysis of a group of target genes, entire protein coding regions of the human genome, and the whole human genome has become a reality. Next Generation Sequencing (NGS) or Massively Parallel Sequencing (MPS) technologies offers a way to screen for mutations in many different genes in a cost and time efficient manner by deep coverage of the target sequences. This novel technology has now been applied to clinical diagnosis of Mendelian disorders of well characterized or undefined diseases, discovery of new disease genes, noninvasive prenatal diagnosis using maternal blood, and population based carrier testing of severe autosomal recessive disorders. This book covers topics of these applications, including potential limitations and expanded application in the future. ​

Author : Urszula Demkow,Rafal Ploski
Publisher : Academic Press
Page : 334 pages
File Size : 46,8 Mb
Release : 2015-09-10
Category : Science
ISBN : 9780128018415

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Clinical Applications for Next-Generation Sequencing by Urszula Demkow,Rafal Ploski Pdf

Clinical Applications for Next Generation Sequencing provides readers with an outstanding postgraduate resource to learn about the translational use of NGS in clinical environments. Rooted in both medical genetics and clinical medicine, the book fills the gap between state-of-the-art technology and evidence-based practice, providing an educational opportunity for users to advance patient care by transferring NGS to the needs of real-world patients. The book builds an interface between genetic laboratory staff and clinical health workers to not only improve communication, but also strengthen cooperation. Users will find valuable tactics they can use to build a systematic framework for understanding the role of NGS testing in both common and rare diseases and conditions, from prenatal care, like chromosomal abnormalities, up to advanced age problems like dementia. Fills the gap between state-of-the-art technology and evidence-based practice Provides an educational opportunity which advances patient care through the transfer of NGS to real-world patient assessment Promotes a practical tool that clinicians can apply directly to patient care Includes a systematic framework for understanding the role of NGS testing in many common and rare diseases Presents evidence regarding the important role of NGS in current diagnostic strategies

Author : Elena Pilli,Andrea Berti
Publisher : CRC Press
Page : 479 pages
File Size : 49,9 Mb
Release : 2021-03-31
Category : Law
ISBN : 9781000064582

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Forensic DNA Analysis by Elena Pilli,Andrea Berti Pdf

Forensic DNA Analysis: Technological Development and Innovative Applications provides a fascinating overview of new and innovative technologies and current applications in forensic genetics. Edited by two forensic experts with many years of forensic crime experience with the Italian police and with prestigious academic universities, the volume takes an interdisciplinary perspective, the volume presents an introduction to genome polymorphisms, discusses, forensic genetic markers, presents a variety of new methods and techniques in forensic genetics, and looks at a selection of new technological innovations and inventions now available from commercial vendors. The book is an important resource for scientists, researchers, and other experts in the field who will find it of interest for its exhaustive discussion of the most important technological innovations in forensic genetics. For those newer to the field, the volume will be an invaluable reference guide to the forensic world.

Author : Stuart M. Brown
Publisher : Unknown
Page : 0 pages
File Size : 54,8 Mb
Release : 2015
Category : MEDICAL
ISBN : 1621821234

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Next-generation DNA Sequencing Informatics by Stuart M. Brown Pdf

"Next-generation DNA sequencing (NGS) technology has revolutionized biomedical research, making complete genome sequencing an affordable and frequently used tool for a wide variety of research applications. This book provides a thorough introduction to the necessary informatics methods and tools for operating NGS instruments and analyzing NGS data"

Author : Sara El-Metwally,Osama M. Ouda,Mohamed Helmy
Publisher : Springer Science & Business
Page : 118 pages
File Size : 49,6 Mb
Release : 2014-04-19
Category : Science
ISBN : 9781493907151

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Next Generation Sequencing Technologies and Challenges in Sequence Assembly by Sara El-Metwally,Osama M. Ouda,Mohamed Helmy Pdf

The introduction of Next Generation Sequencing (NGS) technologies resulted in a major transformation in the way scientists extract genetic information from biological systems, revealing limitless insight about the genome, transcriptome and epigenome of any species. However, with NGS, came its own challenges that require continuous development in the sequencing technologies and bioinformatics analysis of the resultant raw data and assembly of the full length genome and transcriptome. Such developments lead to outstanding improvements of the performance and coverage of sequencing and improved quality for the assembled sequences, nevertheless, challenges such as sequencing errors, expensive processing and memory usage for assembly and sequencer specific errors remains major challenges in the field. This book aims to provide brief overviews the NGS field with special focus on the challenges facing the NGS field, including information on different experimental platforms, assembly algorithms and software tools, assembly error correction approaches and the correlated challenges.

Author : Hirak Ranjan Dash,Kelly M. Elkins,Noora Rashid Al-Snan
Publisher : Elsevier
Page : 614 pages
File Size : 51,8 Mb
Release : 2023-11-30
Category : Science
ISBN : 9780323993807

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Next Generation Sequencing (NGS) Technology in DNA Analysis by Hirak Ranjan Dash,Kelly M. Elkins,Noora Rashid Al-Snan Pdf

Next Generation Sequencing (NGS) Technology in DNA Analysis explains and summarizes next generation sequencing (NGS) technological applications in the field of forensic DNA analysis. The book covers the transition from capillary electrophoresis (CE)-based technique to NGS platforms and the fundamentals of NGS technologies, applications, and advances. Sections provide an overview of NGS technology and forensic science, including information on processing biological samples for forensic analysis, sequence analysis, and data analysis software as well as the analysis of NGS data. The book explores the valuable applications of NGS-based forensic DNA analysis and covers the validations and interpretation guidelines of NGS workflows. With chapter contributions from an international array of experts and the inclusion of practical case studies, this book is a useful reference for academicians and researchers in genetics, biotechnology, bioinformatics, biology, and medicine as well as forensic DNA scientists and practitioners who aim to learn, use, apply, and validate NGS-based technologies. Describes the fundamentals of NGS and its advances for forensic applications Explains the transition from CE-based technique to NGS technology Includes case studies related to NGS and DNA fingerprinting Explores the future use and applications of NGS technologies

Author : Xinkun Wang
Publisher : CRC Press
Page : 258 pages
File Size : 50,7 Mb
Release : 2016-04-06
Category : Mathematics
ISBN : 9781482217896

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Next-Generation Sequencing Data Analysis by Xinkun Wang Pdf

A Practical Guide to the Highly Dynamic Area of Massively Parallel SequencingThe development of genome and transcriptome sequencing technologies has led to a paradigm shift in life science research and disease diagnosis and prevention. Scientists are now able to see how human diseases and phenotypic changes are connected to DNA mutation, polymorphi

Author : Somnath Datta,Dan Nettleton
Publisher : Springer
Page : 438 pages
File Size : 50,6 Mb
Release : 2014-07-03
Category : Medical
ISBN : 9783319072128

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Statistical Analysis of Next Generation Sequencing Data by Somnath Datta,Dan Nettleton Pdf

Next Generation Sequencing (NGS) is the latest high throughput technology to revolutionize genomic research. NGS generates massive genomic datasets that play a key role in the big data phenomenon that surrounds us today. To extract signals from high-dimensional NGS data and make valid statistical inferences and predictions, novel data analytic and statistical techniques are needed. This book contains 20 chapters written by prominent statisticians working with NGS data. The topics range from basic preprocessing and analysis with NGS data to more complex genomic applications such as copy number variation and isoform expression detection. Research statisticians who want to learn about this growing and exciting area will find this book useful. In addition, many chapters from this book could be included in graduate-level classes in statistical bioinformatics for training future biostatisticians who will be expected to deal with genomic data in basic biomedical research, genomic clinical trials and personalized medicine. About the editors: Somnath Datta is Professor and Vice Chair of Bioinformatics and Biostatistics at the University of Louisville. He is Fellow of the American Statistical Association, Fellow of the Institute of Mathematical Statistics and Elected Member of the International Statistical Institute. He has contributed to numerous research areas in Statistics, Biostatistics and Bioinformatics. Dan Nettleton is Professor and Laurence H. Baker Endowed Chair of Biological Statistics in the Department of Statistics at Iowa State University. He is Fellow of the American Statistical Association and has published research on a variety of topics in statistics, biology and bioinformatics.

Author : Ali Masoudi-Nejad,Zahra Narimani,Nazanin Hosseinkhan
Publisher : Springer Science & Business Media
Page : 86 pages
File Size : 54,5 Mb
Release : 2013-07-09
Category : Medical
ISBN : 9781461477266

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Next Generation Sequencing and Sequence Assembly by Ali Masoudi-Nejad,Zahra Narimani,Nazanin Hosseinkhan Pdf

The goal of this book is to introduce the biological and technical aspects of next generation sequencing methods, as well as algorithms to assemble these sequences into whole genomes. The book is organized into two parts; part 1 introduces NGS methods and part 2 reviews assembly algorithms and gives a good insight to these methods for readers new to the field. Gathering information, about sequencing and assembly methods together, helps both biologists and computer scientists to get a clear idea about the field. Chapters will include information about new sequencing technologies such as ChIp-seq, ChIp-chip, and De Novo sequence assembly. ​

Author : Xiu-An Yang,Hu Hao,Yanling Yang,Can Liao
Publisher : Frontiers Media SA
Page : 104 pages
File Size : 49,6 Mb
Release : 2022-01-28
Category : Science
ISBN : 9782889742035

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Next Generation Sequencing (NGS) for Rare Diseases Diagnosis by Xiu-An Yang,Hu Hao,Yanling Yang,Can Liao Pdf