Nucleic Acid Aptamers

Author : Günter Mayer,Marcus M. Menger
Publisher : Springer Nature
Page : 283 pages
File Size : 50,5 Mb
Release : 2022-09-26
Category : Science
ISBN : 9781071626955

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Nucleic Acid Aptamers by Günter Mayer,Marcus M. Menger Pdf

This updated book reflects improvements in a variety of techniques used to study the aptamer field. Beginning with a section on selection procedures, the volume continues with methods to characterize aptamers’ interaction and structural properties by biophysical approaches, as well as a variety of applications that have been adapted to the aptamer compound class. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Nucleic Acid Aptamers: Selection, Characterization, and Application, Second Edition serves as an ideal guide for researchers aiming to further our understanding of aptamer biology and more.

Author : Günter Mayer
Publisher : Humana
Page : 0 pages
File Size : 45,7 Mb
Release : 2015-11-10
Category : Science
ISBN : 1493931962

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Nucleic Acid Aptamers by Günter Mayer Pdf

This volume provides protocol references covering recent developments in the aptamer field. Within the last decade, aptamers have become more and more popular, and their sophisticated biophysical properties together with their ability to be easily modified and, thus, adapted to various regimens makes them a very promising class of compounds. Divided into three sections, the book covers selection, a series of analytical methods to assess biophysical properties of aptamer-target interactions, as well as various applications of aptamers. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to follow, Nucleic Acid Aptamers: Selection, Characterization, and Application provides a state-of-the-art summary of recent developments in the aptamer field and will be a helpful resource for scientists in the life sciences working with aptamers as tools to elucidate biological systems.

Author : Sven Klussmann
Publisher : John Wiley & Sons
Page : 518 pages
File Size : 43,6 Mb
Release : 2006-08-21
Category : Science
ISBN : 9783527607914

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The Aptamer Handbook by Sven Klussmann Pdf

In The Aptamer Handbook, leading scientists from academia as well as biotech and pharma companies introduce the revolutionary concept of designing RNA and DNA oligonucleotides with novel functions by in vitro selection. These functions comprise high affinity binding (aptamers), catalytic activity (ribozymes and deoxyribozymes) or combinations of binding and catalytic properties (aptazymes). Basic concepts and technologies describing in detail how these functional oligonucleotides can be identified are presented. Numerous examples demonstrate the versatility of in vitro selected oligonucleotides. Special emphasis has been put on a section that shows the broad applicability of aptamers, e. g. in target validation, for analytics, or as new therapeutics. This first overview in the field is of prime interest for a broad audience of scientists both in academia and in industry who wish to expand their knowledge on the potential of new oligonucleotide functions and their applications.

Author : Günter Mayer
Publisher : Humana Press
Page : 0 pages
File Size : 53,8 Mb
Release : 2014-12-04
Category : Science
ISBN : 1627038930

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Nucleic Acid and Peptide Aptamers by Günter Mayer Pdf

After the deciphering of the human genome and the genomes of many other organisms, the investigation of the function of gene products and their orchestral interplay is now one of the most important challenges in the life sciences. In "Nucleic Acid and Peptide Aptamers: Methods and Protocols", expert researchers contribute state-of-the-art methods focused on these two vital molecule types which are so often employed for in vitro selection procedures. Divided conveniently into two distinct parts beginning with nucleic acid aptamers and ending with peptide aptamers, the volume provides methodologies for the isolation, characterization, and application of both types. Written in the highly successful Methods in Molecular BiologyTM series format, all chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy to use, "Nucleic Acid and Peptide Aptamers: Methods and Protocols" will provide researchers with an inspiring and helpful guide to the application of these compounds to their own distinct research issues.

Author : Rakesh N. Veedu
Publisher : CRC Press
Page : 386 pages
File Size : 45,5 Mb
Release : 2017-03-03
Category : Medical
ISBN : 9789814669849

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Aptamers by Rakesh N. Veedu Pdf

Aptamers, often termed as ‘chemical antibodies,’ are an emerging class of synthetic ligands for efficient target-specific molecular recognition. The objective of this book is to highlight recent advances and potential of aptamers in various disease conditions. . This book focuses on the applications of aptamers in targeted nanotherapy, detection, and in molecular imaging in various disease conditions such as cancer, neurological diseases and infectious diseases.

Author : Julian Alexander Tanner, Andrew Brian Kinghorn, Yee-Wai Cheung
Publisher : MDPI
Page : 301 pages
File Size : 45,7 Mb
Release : 2018-08-27
Category : Electronic
ISBN : 9783038970590

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Aptamers by Julian Alexander Tanner, Andrew Brian Kinghorn, Yee-Wai Cheung Pdf

This book is a printed edition of the Special Issue " Aptamers" that was published in IJMS

Author : Gulab Singh Yadav,Vikas Kumar,Neeraj K. Aggarwal
Publisher : Springer Nature
Page : 186 pages
File Size : 51,9 Mb
Release : 2019-11-11
Category : Science
ISBN : 9789811388361

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Aptamers by Gulab Singh Yadav,Vikas Kumar,Neeraj K. Aggarwal Pdf

The book discusses the basics of aptamers and the advent of aptamer-based technology in recent times. The book covers the diverse applications of aptamers, such as in detection of animal and plant pathogens, disease diagnosis and therapeutics, environmental contamination detection etc. Besides these applications, the book also describes the use of these synthetic or modified DNA, as drug delivery vehicles. The different chapters describe how the binding capacity and specificity of aptamers can be exploited in various ways. The book also discusses how these attributes of aptamers can outdo the antibody technology in biomedical and diagnostic solutions. This crisp and concise book gives the readers an insight into the most recent biotechnological applications of aptamers. ​

Author : Katharina Urmann,Johanna-Gabriela Walter
Publisher : Springer Nature
Page : 217 pages
File Size : 45,5 Mb
Release : 2020-07-31
Category : Science
ISBN : 9783030540616

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Aptamers in Biotechnology by Katharina Urmann,Johanna-Gabriela Walter Pdf

This book reviews the development, characterization and applications of aptamers in different areas of biotechnology ranging from therapeutics to diagnostics and protein purification. Hailed as chemical antibodies, these single-stranded nucleic acid receptors were predicted to supersede antibodies in traditional assays, such as ELISA, within a short time. While this has yet to happen, readers will find in this book a deep insight into the progress of aptamer technology and a critical discussion about the limitations that need to be overcome in order to find wider acceptance and use outside of the still relatively small aptamer-community. This book covers all aspects of aptamer generation and application for the aptamer-experienced reader and curious novice alike, with the addition of an industry perspective on the future of aptamer-use in biotechnology.

Author : Michael Famulok,Ernst-L. Winnacker,Chi-Huey Wong
Publisher : Springer Science & Business Media
Page : 199 pages
File Size : 41,6 Mb
Release : 2013-06-29
Category : Medical
ISBN : 9783642601422

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Combinatorial Chemistry in Biology by Michael Famulok,Ernst-L. Winnacker,Chi-Huey Wong Pdf

The essence of combinatorial chemistry or techniques involving "molecular diversity" is to generate enormous populations of molecules and to exploit appropriate screening techniques to isolate active components contained in these libraries. This idea has been the focus of research both in academia and in the pharmaceutical or biotechnology industry. Its developments go hand in hand with an exploding number of potential drug targets emerging from genomics and proteomics research. When the editors of Current Topics in Microbiology and Immunology encouraged us to assemble the present volume on Combinatorial Chemistry in Biology, we immediately felt that this might prove quite beneficial for the audience of this series. The field of combinatorial chemistry extends over a broad range of disciplines, from synthetic organic chemistry to biochemistry, from material sciences to cell biology. Each of these fields may have its own view on this topic, something which is reflected in a growing number of monographs and "special editions" of jour nals devoted to this issue or aspects thereof. The title of the present volume of Springer-Verlag's series suggests that it also has its own special focus. And, generally speaking, this is not wrong: we would even claim the special focus of this volume is on the immunologically relevant aspects of combinatorial chemistry.

Author : Yiyang Dong
Publisher : John Wiley & Sons
Page : 426 pages
File Size : 44,7 Mb
Release : 2019-02-11
Category : Science
ISBN : 9783527342679

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Aptamers for Analytical Applications by Yiyang Dong Pdf

An essential guide that puts the focus on method developments and applications in aptamers In recent years, aptamer-based systems have been developed for a wide-range of analytical and medical applications. Aptamers for Analytical Applications offers an introduction to the topic, outlines the common protocols for aptamer synthesis, as well as providing information on the different optimization strategies that can obtain higher affinities to target molecules. The contributors?noted experts on the topic?provide an in-depth review of the characterization of aptamer-target molecule interaction and immobilization strategies and discuss the developments of methods for all the relevant applications. The book outlines different schemes to efficiently immobilize aptamers on substrates as well as summarizing the characterization methods for aptamer-ligand complexes. In addition, aptamer-based colorimetric, enzyme-linked, fluorescent, electrochemical, lateral flow and non-labeling analytical methods are presented. The book also reflects state-of-the-art and emerging applications of aptamer-based methods. This important resource: -Provides a guide to aptamers which provide highly specific and sensitive molecular recognition, with affinities in the range of antibodies and are much cheaper to produce -Offers a discussion of the analytical method developments and improvements with established systems and beyond -Offers a comprehensive guide to all the relevant application areas -Presents an authoritative book from contributors who are noted experts in the field Written for analytical chemists, biochemists, analytical researchers, Aptamers for Analytical Applications is a comprehensive book that adopts a methodological point of view to the important aspects of aptamer generation and modification with a strong emphasis on method developments for relevant applications.

Author : Weihong Tan,Xiaohong Fang
Publisher : Springer
Page : 352 pages
File Size : 55,9 Mb
Release : 2015-03-02
Category : Science
ISBN : 9783662462263

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Aptamers Selected by Cell-SELEX for Theranostics by Weihong Tan,Xiaohong Fang Pdf

This edited volume describes cell-SELEX as the fundamental tool used to generate aptamer molecules for a wide range of applications in molecular medicine, bioanalysis and chemical biology. Easily integrated into the natural heterogeneous cell matrix, aptamers can be effectively used in theranostics, bioanalysis, environment detection and biomedical studies. The book gathers reviews that reflect the latest advances in the field of aptamers and consists in fourteen chapters demonstrating essential examples of these aptamers and aptamer-nanomaterial assemblies, depending on the types of applications and biological systems. It also includes a separate chapter on the utilization of aptamers in real clinics and what will be required to achieve this significant goal. The book will be both appealing and useful to a broad audience, including biologists, bioscientists, and clinicians whose interests range from chemistry and biomedical engineering to cell and molecular biology and biotechnology. Weihong Tan is a Distinguished Professor of Chemistry and Biomedical Engineering at Hunan University, China and also a University of Florida Distinguished Professor and V.T. and Louis Jackson Professor of Chemistry at the University of Florida, USA. Xiaohong Fang is a Professor at the Institute of Chemistry, Chinese Academy of Sciences, China.

Author : Yingfu Li,Yi Lu
Publisher : Springer Science & Business Media
Page : 396 pages
File Size : 49,6 Mb
Release : 2009-05-27
Category : Science
ISBN : 9780387737119

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Functional Nucleic Acids for Analytical Applications by Yingfu Li,Yi Lu Pdf

Nature has long used nucleic acid aptamers and enzymes for regulatory activities, such as the recently discovered “riboswitches” involved in gene expression. The existence of a large array of natural and artificial functional nucleic acids has generated tremendous enthusiasm and new opportunities for molecular scientists from diverse disciplines to devise new concepts and real applications that take advantage of those nucleic acids for sensing and other analytical applications. This book provides a timely and comprehensive overview of recent advances in the field, from leading experts in biology, chemistry, and engineering. A variety of topics are covered, from fundamentals of functional nucleic acids, to their applications as sensors, to nanotechnologies; as well as integration of functional nucleic acids into practical analytical systems.

Author : Marcelo Larramendy,Sonia Soloneski
Publisher : BoD – Books on Demand
Page : 222 pages
File Size : 45,9 Mb
Release : 2016-03-16
Category : Science
ISBN : 9789535122647

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Nucleic Acids by Marcelo Larramendy,Sonia Soloneski Pdf

This edited book, "Nucleic Acids - From Basic Aspects to Laboratory Tools", contains a series of chapters that highlight the development and status of the various aspects of the nucleic acids related to DNA chemistry and biology and the molecular application of these small DNA molecules and related synthetic analogues within biological systems. Furthermore, it is hoped that the information in the present book will be of value to those directly engaged in the handling and use of nucleic acids, and that this book will continue to meet the expectations and needs of all who are interested in the different fascinating aspects of molecular biology.

Author : Akira Murakami
Publisher : Springer
Page : 189 pages
File Size : 53,6 Mb
Release : 2012-07-04
Category : Technology & Engineering
ISBN : 9783642304637

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Nucleic Acid Drugs by Akira Murakami Pdf

New Antisense Strategies: Chemical Synthesis of RNA Oligomers, by Junichi Yano und Gerald E. Smyth Development and Modification of Decoy Oligodeoxynucleotides for Clinical Application, by Mariana Kiomy Osako, Hironori Nakagami und Ryuichi Morishita Modulation of Endosomal Toll-Like Receptor-Mediated Immune Responses by Synthetic Oligonucleotides, by Ekambar R. Kandimalla und Sudhir Agrawal Delivery of Nucleic Acid Drugs, by Yan Lee und Kazunori Kataoka Aptamer: Biology to Applications, by Yoshikazu Nakamura Development and Clinical Applications of Nucleic Acid Therapeutics, by Veenu Aishwarya, Anna Kalota und Alan M. Gewirtz

Author : BCC Research
Publisher : Unknown
Page : 128 pages
File Size : 40,8 Mb
Release : 2012-10
Category : Electronic
ISBN : 0893361631

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Nucleic Acid Aptamers for Diagnostics and Therapeutics by BCC Research Pdf