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Author : Philip H. Jones,Onofrio M. Marag-,Giovanni Volpe
Publisher : Cambridge University Press
Page : 565 pages
File Size : 49,6 Mb
Release : 2015-12-03
Category : Science
ISBN : 9781107051164
A comprehensive guide to the theory, practice and applications of optical tweezers, combining state-of-the-art research with a strong pedagogic approach.
Author : Miles J. Padgett,Justin Molloy,David McGloin
Publisher : CRC Press
Page : 508 pages
File Size : 52,9 Mb
Release : 2010-06-02
Category : Technology & Engineering
ISBN : 9781420074147
The technical development of optical tweezers, along with their application in the biological and physical sciences, has progressed significantly since the demonstration of an optical trap for micron-sized particles based on a single, tightly focused laser beam was first reported more than twenty years ago. Bringing together many landmark papers on the field, Optical Tweezers: Methods and Applications covers the techniques and uses of optical tweezers. Each section is introduced by a brief commentary, setting the papers into their historical and contemporary contexts. The first two sections explore the pioneering work of Arthur Ashkin and the use of optical tweezers in biological systems. The book then discusses the extensive use of optical tweezers for the measurement of picoNewton forces and examines various approaches for modeling forces within optical tweezers. The next parts explain how optical tweezers are used in colloid science, how to convert optical tweezers into optical spanners, and how spatial light modulators create holographic tweezers. The book concludes with a section on emerging applications of optical tweezers in microfluidic systems. With contributions from some of the best in the field, this compendium presents important historical and current developments of optical tweezers in a range of scientific areas, from the manipulation of bacteria to the treatment of DNA.
Author : Arne Gennerich
Publisher : Humana
Page : 0 pages
File Size : 48,8 Mb
Release : 2016-11-15
Category : Science
ISBN : 1493964194
The aim of this volume is to provide a comprehensive overview of optical tweezers setups, both in practical and theoretical terms, to help biophysicists, biochemists, and cell biologists to build and calibrate their own instruments and to perform force measurements on mechanoenzymes both in isolation in vitro and in living cells. Chapters have been divided in three parts focusing on theory and practical design of optical tweezers, detailed protocols for performing force measurements on single DNA- and microtubule/actin-associated mechanoenzymes in isolation, and describing recent advances that have opened up quantitative force measurements in living cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Optical Tweezers: Methods and Protocols aims help to further expand the accessibility and use of optical traps by scientists of diverse disciplines.
Author : Holger Kress
Publisher : Cuvillier Verlag
Page : 155 pages
File Size : 43,5 Mb
Release : 2006
Category : Electronic
ISBN : 9783867270076
Author : Arne Gennerich
Publisher : Springer Nature
Page : 753 pages
File Size : 44,9 Mb
Release : 2022-09-05
Category : Science
ISBN : 9781071622292
This detailed volume explores a wide variety of techniques involving optical tweezers, a technology that has become increasingly more accessible to a broad range of researchers. Beginning with recent technical advances, the book continues by covering the application of optical tweezers to study DNA-protein interactions and DNA motors, protocols to perform protein (un)folding experiments, the application of optical tweezers to study actin- and microtubule-associated motor proteins, and well as protocols for investigating the function and mechanical properties of microtubules and intermediate filaments, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Optical Tweezers: Methods and Protocols, Second Edition serves as an ideal resource for expanding the accessibility and use of optical traps by scientists of diverse disciplines.
Author : Manlio Tassieri
Publisher : CRC Press
Page : 328 pages
File Size : 47,5 Mb
Release : 2016-10-14
Category : Science
ISBN : 9781315341224
Thanks to the pioneering works of Ashkin and coworkers, optical tweezers (OTs) have become an invaluable tool for myriad studies throughout the natural sciences. Their success relies on the fact that they can be considered as exceptionally sensitive transducers that are able to resolve pN forces and nm displacements, with high temporal resolution, down to μs. Hence their application to study a wide range of biological phenomena such as measuring the compliance of bacterial tails, the forces exerted by a single motor protein, and the mechanical properties of human red blood cells and of individual biological molecules. The number of articles related to them totals to a whopping 58,000 (source Google Scholar)! Microrheology is a branch of rheology, but it works at micrometer length scales and with microliter sample volumes. Therefore, microrheology techniques have been revealed to be very useful tools for all those rheological/mechanical studies where rare or precious materials are employed, such as in biological and biomedical studies. The aim of this book is to provide a pedagogical introduction to the physics principles governing both the optical tweezers and their application in the field of microrheology of complex materials. This is achieved by following a linear path that starts from a narrative introduction of the "nature of light," followed by a rigorous description of the fundamental equations governing the propagation of light through matter. Moreover, some of the many possible instrumental configurations are presented, especially those that better adapt to perform microrheology measurements. In order to better appreciate the microrheological methods with optical tweezers explored in this book, informative introductions to the basic concepts of linear rheology, statistical mechanics, and the most popular microrheology techniques are also given. Furthermore, an enlightening prologue to the general applications of optical tweezers different from rheological purposes is provided at the end of the book.
Author : Zhenjiang Ni,Céline Pacoret,Ryad Benosman,Stéphane Régnier
Publisher : John Wiley & Sons
Page : 205 pages
File Size : 53,9 Mb
Release : 2014-11-10
Category : Technology & Engineering
ISBN : 9781848216952
The authors of this book provide the first review of haptic optical tweezers, a new technique which brings together force feedback teleoperation and optical tweezers. This technique allows users to explore the microworld by sensing and exerting piconewton-scale forces with trapped microspheres. The design of optical tweezers for high-quality haptic feedback is challenging, given the requirements for very high sensitivity and dynamic stability. The concept, design process and specification of optical tweezers reviewed throughout this book focus on those intended for haptic teleoperation. The authors provide two new specific designs as well as the current state of the art. Furthermore, the remaining important issues are identified for further developments. Haptic optical tweezers will soon become an invaluable tool for force feedback micromanipulation of biological samples and nano- and micro-assembly parts.
Author : Michael W. Berns,Daryl Preece,Halina Rubinsztein-Dunlop,Monika Ritsch-Marte
Publisher : Frontiers Media SA
Page : 362 pages
File Size : 46,6 Mb
Release : 2022-02-03
Category : Science
ISBN : 9782889742431
Author : Marius Martin
Publisher : GRIN Verlag
Page : 37 pages
File Size : 50,8 Mb
Release : 2023-03-30
Category : Science
ISBN : 9783346846976
Bachelor Thesis from the year 2023 in the subject Physics - Applied physics, University of Aberdeen, language: English, abstract: This thesis investigates two powerful techniques for manipulating and studying microscopic particles: optical tweezers and acoustic levitation. Optical tweezers use the force of light to trap and manipulate small particles, while acoustic levitation uses sound waves to levitate particles in mid-air. The first part of the thesis focuses on the construction and optimization of an optical tweezers system. The system is build using high-powered laser, optics for focusing the laser beam, and a position detection and control system. the second part of this thesis explores the use of acoustic levitation for the manipulation of micro spheres. The acoustic levitation system is designed and constructed using 72 transducers. The system is characterized using measurements of the acoustic field and the resulting levitation forces. The levitation of micro spheres is demonstrated. This thesis would provide a detailed investigation of two powerful techniques for the manipulation of microscopic particles.
Author : Philip Jones,Daniel R. Burnham
Publisher : MDPI
Page : 128 pages
File Size : 40,5 Mb
Release : 2020-11-25
Category : Technology & Engineering
ISBN : 9783039435371
We are pleased to present “Optical Trapping and Manipulation: From Fundamentals to Applications”, a Special Issue of Micromachines dedicated to the latest research in optical trapping. In recognition of the broad impact of optical manipulation techniques across disciplines, this Special Issue collected contributions related to all aspects of optical trapping and manipulation. Both theoretical and experimental studies were welcome, and applications of optical manipulation methods in fields including (but not limited to) single molecule biophysics, cell biology, nanotechnology, atmospheric chemistry, and fundamental optics were particularly welcome in order to showcase the breadth of the current research. The Special Issue accepted diverse forms of contributions, including research papers, short communications, methods, and review articles representing the state-of-the-art in optical trapping.
Author : Anonim
Publisher : Academic Press
Page : 228 pages
File Size : 55,5 Mb
Release : 1997-11-24
Category : Science
ISBN : 0080859534
Volume 55 in Methods in Cell Biology is a concise laboratory book that emphasizes the methods and technologies needed to use single polarized laser light source that functions simultaneously as an optical trap and a dual-beam interferometer. * * Provides a practical laboratory guide for methods and technologies used with laser tweezers * Includes comprehensive and easy-to-follow protocols
Author : Mike Wördemann
Publisher : Springer Science & Business Media
Page : 139 pages
File Size : 48,9 Mb
Release : 2012-05-16
Category : Science
ISBN : 9783642293238
The optical trapping of colloidal matter is an unequalled field of technology for enabling precise handling of particles on microscopic scales, solely by the force of light. Although the basic concept of optical tweezers, which are based on a single laser beam, has matured and found a vast number of exciting applications, in particular in the life sciences, there are strong demands for more sophisticated approaches. This thesis gives an introductory overview of existing optical micromanipulation techniques and reviews the state-of-the-art of the emerging field of structured light fields and their applications in optical trapping, micromanipulation, and organisation. The author presents established, and introduces novel concepts for the holographic and non-holographic shaping of a light field. A special emphasis of the work is the demonstration of advanced applications of the thus created structured light fields in optical micromanipulation, utilising various geometries and unconventional light propagation properties. While most of the concepts developed are demonstrated with artificial microscopic reference particles, the work concludes with a comprehensive demonstration of optical control and alignment of bacterial cells, and hierarchical supramolecular organisation utilising dedicated nanocontainer particles.
Author : Peter Hinterdorfer,Antoine van Oijen
Publisher : Springer Science & Business Media
Page : 634 pages
File Size : 42,8 Mb
Release : 2009-12-24
Category : Science
ISBN : 9780387764979
This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.
Author : Taylor & Francis
Publisher : Unknown
Page : 136 pages
File Size : 44,6 Mb
Release : 2003
Category : Laser beams
ISBN : OCLC:52592343
Author : Michael Esseling
Publisher : Springer
Page : 125 pages
File Size : 54,8 Mb
Release : 2014-07-31
Category : Science
ISBN : 9783319093185
In the never-ending quest for miniaturization, optically controlled particle trapping has opened up new possibilities for handling microscopic matter non-invasively. This thesis presents the application of photorefractive crystals as active substrate materials for optoelectronic tweezers. In these tweezers, flexible optical patterns are transformed into electrical forces by a photoconductive material, making it possible to handle matter with very high forces and high throughput. Potential substrate materials’ properties are investigated and ways to tune their figures-of-merit are demonstrated. A large part of the thesis is devoted to potential applications in the field of optofluidics, where photorefractive optoelectronic tweezers are used to trap, sort and guide droplets or particles in microfluidic channels, or to shape liquid polymers into optical elements prior to their solidification. Furthermore, a new surface discharge model is employed to discuss the experimental conditions needed for photorefractive optoelectronic tweezers.