Author : C. S. Herrington
Publisher : Unknown
Page : 205 pages
File Size : 50,9 Mb
Release : 1997
Category : Electronic
ISBN : OCLC:1123626089
PCR 3
C. S. Herrington
Pcr In Situ Hybridization
Pcr In Situ Hybridization Book in PDF, ePub and Kindle version is available to download in english. Read online anytime anywhere directly from your device. Click on the download button below to get a free pdf file of Pcr In Situ Hybridization book. This book definitely worth reading, it is an incredibly well-written.
PCR 3
C. Simon Herrington,John J. O'Leary
Author : C. Simon Herrington,John J. O'Leary
Publisher : Oxford University Press
Page : 223 pages
File Size : 51,8 Mb
Release : 1997-10-30
Category : Science
ISBN : 9780191565595
PCR 3 by C. Simon Herrington,John J. O'Leary Pdf
PCR in situ hybridization allows the detection of specific nucleic acid sequences and their distribution in the cell. It combines two powerful techniquesin situ hybridization (ISH), which allows cellular localization of DNA and RNA sequences in cells and tissues, and the polymerase chain reaction (PCR), which allows reproducible amplification of rare nucleic acid sequences. The combined technique and its variants greatly enhance the sensitivity of in situ hybridization and add morphological localization to the sensitivity of PCR. Such techniques have enormous potential for research and diagnosis but problems with reproducibility and reliability are often encountered. This book overcomes these problems by describing the key procedures in step-by-step detail and by providing the essential advice needed for success. Topics include: DNA in situ PCR and DNA PCR in situ hybridization (PCR ISH) for the detection of DNA targets in cells; reverse trancriptase in situ PCR (RT-PCR) and RT-PCR ISH for the detection of RNA targets; and PRINS (primed in situ synthesis) for chromosomal analysis in interphase nuclei and metaphase chromosome spreads. There are further chapters on fixation of tissues for PCR, selective ultraviolet radiation fractionation (SURF), application of in situ PCR to human tissues, applications and modifications of PCR-ISH, and automation of in situ amplification. PCR In Situ Hybridization is a unique and timely collection of well-tested protocols for the amplification of DNA and RNA in cells and tissues, drawing on the accumulated knowledge and experience of leading exponents of these techniques. For each topic covered, the authors provide detailed guidance on the key steps in the protocols, numerous hints and tips for success, and advice on trouble-shooting. PCR In Situ Hybridization will be invaluable to molecular biologists, pathologists, geneticists, and all those seeking to perform in situ analyses of nucleic acid molecules.
PCR in Situ Hybridization
Gerard J. Nuovo
Author : Gerard J. Nuovo
Publisher : Lippincott Williams & Wilkins
Page : 536 pages
File Size : 53,9 Mb
Release : 1997
Category : Cytodiagnosis
ISBN : UOM:39015038533090
PCR in Situ Hybridization by Gerard J. Nuovo Pdf
Describes the technique whereby the extreme sensitivity of the polymerase chain reaction (PCR) is combined with the cell localizing ability of in situ hybridization. This revised and updated edition contains chapters on the basics of molecular biology; the nonspecific pathways of PCR; applications of PCR in situ hybridization--human papillomavirus, and HIV-1; and instrumentation. There is also an appendix on reagents for molecular biological analyses. Annotation copyright by Book News, Inc., Portland, OR
PCR 3
C. S. Herrington,John J. O'Leary
Author : C. S. Herrington,John J. O'Leary
Publisher : Oxford University Press, USA
Page : 226 pages
File Size : 54,8 Mb
Release : 1998
Category : DNA replication
ISBN : 9780199636327
PCR 3 by C. S. Herrington,John J. O'Leary Pdf
PCR in situ hybridization allows the detection of specific nucleic acid sequences and their distribution in the cell. It combines two powerful techniquesin situ hybridization (ISH), which allows cellular localization of DNA and RNA sequences in cells and tissues, and the polymerase chainreaction (PCR), which allows reproducible amplification of rare nucleic acid sequences. The combined technique and its variants greatly enhance the sensitivity of in situ hybridization and add morphological localization to the sensitivity of PCR. Such techniques have enormous potential for researchand diagnosis but problems with reproducibility and reliability are often encountered. This book overcomes these problems by describing the key procedures in step-by-step detail and by providing the essential advice needed for success. Topics include: DNA in situ PCR and DNA PCR in situhybridization (PCR ISH) for the detection of DNA targets in cells; reverse trancriptase in situ PCR (RT-PCR) and RT-PCR ISH for the detection of RNA targets; and PRINS (primed in situ synthesis) for chromosomal analysis in interphase nuclei and metaphase chromosome spreads. There are furtherchapters on fixation of tissues for PCR, selective ultraviolet radiation fractionation (SURF), application of in situ PCR to human tissues, applications and modifications of PCR-ISH, and automation of in situ amplification. PCR In Situ Hybridization is a unique and timely collection of well-testedprotocols for the amplification of DNA and RNA in cells and tissues, drawing on the accumulated knowledge and experience of leading exponents of these techniques. For each topic covered, the authors provide detailed guidance on the key steps in the protocols, numerous hints and tips for success,and advice on trouble-shooting. PCR In Situ Hybridization will be invaluable to molecular biologists, pathologists, geneticists, and all those seeking to perform in situ analyses of nucleic acid molecules.
PCR in Situ Hybridization
Anonim
Author : Anonim
Publisher : Unknown
Page : 128 pages
File Size : 42,9 Mb
Release : 2024-06-17
Category : Electronic
ISBN : 039758749X
PCR in Situ Hybridization by Anonim Pdf
In Situ PCR and Related Technology
Jiang Gu
Author : Jiang Gu
Publisher : Birkhäuser
Page : 143 pages
File Size : 43,6 Mb
Release : 2012-02-25
Category : Science
ISBN : 1468468456
In Situ PCR and Related Technology by Jiang Gu Pdf
Ever since the introduction of the polymerase chain reaction (peR) in 1986, morphologists, whose interests lie in the analysis of intact tissue structures, have been attempting to adapt this technique to intact cells or tissue sections to detect low copy numbers of DNA or RNA in situ while preserving tissue morphology. The significance of this objective is obvious. A technique finally materialized in 1990 when Dr. Ashley T. Haase and coworkers published results that used multiple prim ers with complementary tails in intact cells. Since then, a number of laboratories have successfully developed their own versions of the technique. In situ peR is now a well-recognized method that permits the detection of minute quantities of DNA or RNA in intact cells or tissue sections. As a result, morphological analysis of those target nucleotide sequences becomes possible. As anticipated, this ad vancement has led to significant improvement in our understanding of many nor mal and abnormal conditions, and its impact is becoming more evident as time passes. In situ peR has the characteristics of a new landmark in morphologic technol ogy-it is scientifically fascinating and technically challenging. In essence, it is a combination of in situ hybridization and conventional peR. The wealth of litera ture, experience and protocols for the two latter techniques can be applied to in situ peR. In situ peR also has its own unique aspects that were not addressed by the other two techniques.
In Situ Hybridization
David G. Wilkinson
Author : David G. Wilkinson
Publisher : OUP Oxford
Page : 243 pages
File Size : 43,7 Mb
Release : 1998-11-26
Category : Science
ISBN : 9780191565700
In Situ Hybridization by David G. Wilkinson Pdf
In situ hybridization is used to reveal the location of specific nucleic acids sequences on chromosomes or in tissues. Visualization of the location of genes on chromosomes or of specific mRNAs or viruses in tissues is crucial for understanding the organization, regulation, and function of genes. It is a therefore a core technique in all areas of biomedical research. In Situ Hybridization: A Practical Approach 2/e is the second edition of one of the most successful Practical Approach books, published in 1992. Since the first edition was published, a number of important technical advances have been made. The new edition has been thoroughly updated to contain protocols detailing the major techniques of in situ hybridization currently in use: in situ hybridization to mRNA with oligonucleotide and RNA probes (radiolabelled and hapten labelled); analysis using light and electron microscopes; whole mount in situ hybridization; double detection of RNAs, and RNA plus protein; and fluorescent in situ hybridization to detect chromosomal sequences. The protocols are complemented by advice on strategies for successful results, descriptions of the theoretical basis of in situ hybridization and important new developments in gene expression databases. The procedures described are widely applicable to many systems. The use of in situ hybridization in PCR is covered in a separate volume: Herrington and O'Leary (Eds) PCR 3 - PCR in situ hybridization: A Practical Approach (OUP, 1997). All the authors have extensive practical experience of establishing reliable techniques of in situ hybridization. This book will be useful to all researchers at all levels who use in situ hybridization.
PRINS and In Situ PCR Protocols
Franck Pellestor
Author : Franck Pellestor
Publisher : Springer Science & Business Media
Page : 257 pages
File Size : 40,8 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781597450683
PRINS and In Situ PCR Protocols by Franck Pellestor Pdf
The in situ hybridization and PCR technologies are now well-established molecular techniques for studying chromosomal aneuploidy and rearran- ments, gene localization and expression, and genomic organization. Over the last decade, we have seen increasing applications in these fields. By combining the high sensitivity of the PCR reaction and the cytological localization of target sequences, both PRINS and in situ PCR techniques have provided highly powerful complements to FISH for in situ cellular and molecular investigations. Both these approaches have several advantages in terms of sensitivity and specificity, owing to the use of primers and to the fast kinetics of annealing and elongation reactions in situ. In the first edition of PRINS and In Situ PCR Protocols edited by John R. Gosden, experts in the field presented in detail a variety of applications of PRINS and in situ PCR techniques, in a wide range of clinical conditions. Since the publication of this successful reference book, there have been s- nificant improvements in in situ detection techniques. This completely revised and updated second edition presents a compreh- sive selection of new procedures developed in the field of PRINS and in situ PCR technologies. The book has two sections. Part I, Basic Methodology, contains chapters that provide useful protocols for many variations of PRINS and in situ PCR, including a new fast multicolor PRINS method, and protocols for PRINS detection of unique sequences in situ.
Medical BioMethods Handbook
John M. Walker,Ralph Rapley
Author : John M. Walker,Ralph Rapley
Publisher : Springer Science & Business Media
Page : 635 pages
File Size : 47,7 Mb
Release : 2005-03-18
Category : Science
ISBN : 9781588292889
Medical BioMethods Handbook by John M. Walker,Ralph Rapley Pdf
John Walker and Ralph Rapley have collected a wide-ranging group of molecular and biochemical techniques that are the most frequently used in medical and clinical research, especially diagnostics. The authors-well-established investigators who run their own research programs and use the methods on a regular basis-outline the practical procedures for using them and describe a variety of pertinent applications. Among the technologies presented are southern and western blotting, electrophoresis, PCR, cDNA and protein microarrays, liquid chromatography, in situ hybridization, karyotyping, flow cytometry, bioinformatics, genomics, and ribotyping. The applications include assays for mutation detection, mRNA analysis, chromosome translocations, inborn errors of metabolism, protein therapeutics, and gene therapy.
RT-PCR Protocols
Nicola King,Joe O’Connell
Author : Nicola King,Joe O’Connell
Publisher : Springer Science & Business Media
Page : 370 pages
File Size : 40,9 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781592592838
RT-PCR Protocols by Nicola King,Joe O’Connell Pdf
Until the mid 1980s, the detection and quantification of a specific mRNA was a difficult task, usually only undertaken by a skilled molecular biologist. With the advent of PCR, it became possible to amplify specific mRNA, after first converting the mRNA to cDNA via reverse transcriptase. The arrival of this technique—termed reverse transcription-PCR (RT-PCR)—meant that mRNA suddenly became amenable to rapid and sensitive analysis, without the need for advanced training in molecular biology. This new accessibility of mRNA, which has been facilitated by the rapid accumulation of sequence data for human mRNAs, means that every biomedical researcher can now include measurement of specific mRNA expression as a routine component of his/her research plans. In view of the ubiquity of the use of standard RT-PCR, the main objective of RT-PCR Protocols is essentially to provide novel, useful applications of RT-PCR. These include some useful adaptations and applications that could be relevant to the wider research community who are already familiar with the basic RT-PCR protocol. For example, a variety of different adaptations are described that have been employed to obtain quantitative data from RT-PCR. Quantitative RT-PCR provides the ability to accurately measure changes/imb- ances in specific mRNA expression between normal and diseased tissues.
PCR Protocols
John M. S. Bartlett,David Stirling
Author : John M. S. Bartlett,David Stirling
Publisher : Springer Science & Business Media
Page : 1083 pages
File Size : 40,6 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592593842
PCR Protocols by John M. S. Bartlett,David Stirling Pdf
In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.
In Situ Hybridization Methods
Giselbert Hauptmann
Author : Giselbert Hauptmann
Publisher : Humana
Page : 0 pages
File Size : 54,9 Mb
Release : 2016-10-05
Category : Medical
ISBN : 1493944630
In Situ Hybridization Methods by Giselbert Hauptmann Pdf
This volume contains a comprehensive compilation of chromogenic and fluorescent RNA in situ hybridization (ISH) technology in many of its various shades, forms, and applications. The book is organized into a number of parts and chapters focusing on the application of ISH methodologies to different animal species as used in Evolutionary Development (EvoDevo) and Biomedical research, and covering new developments in RNA visualization by fluorescent ISH (FISH). The described (F)ISH protocols employ effective strategies for signal enhancement and target amplification allowing for high signal intensities and drastically improved signal-to-noise ratios. Chromogenic and fluorescent ISH, as specified in the various chapters, are most essential for RNA expression profiling, applied to many fields of research including cellular, developmental, and evolutionary biology, neurobiology and neuropathology. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Essential and authoritative, In Situ Hybridization Methods provides detailed protocols for newcomers to ISH, and inspires researchers familiar with the technique to seek and find up-to-date methodology for new and specialized applications.
In-Situ PCR Techniques
Omar Bagasra,John Hansen
Author : Omar Bagasra,John Hansen
Publisher : Wiley-Liss
Page : 0 pages
File Size : 51,7 Mb
Release : 1997-07-04
Category : Science
ISBN : 0471159468
In-Situ PCR Techniques by Omar Bagasra,John Hansen Pdf
This book describes comprehensive step-by-step protocols for the delineation of genetic amplification and histological detection techniques. Each procedure has been tested and validated for its sensitivity, precision, and reproducibility, and the authors give advice on the design of primers for PCR applications and on optimizing these protocols for use with plant, insect, and prokaryotic cells.
In Situ Hybridization in Neurobiology
James H. Eberwine,Karen L. Valentino,Jack D. Barchas
Author : James H. Eberwine,Karen L. Valentino,Jack D. Barchas
Publisher : Unknown
Page : 230 pages
File Size : 52,9 Mb
Release : 1994
Category : In situ hybridization
ISBN : 0195075072
In Situ Hybridization in Neurobiology by James H. Eberwine,Karen L. Valentino,Jack D. Barchas Pdf
In situ hybridization has become an important and widely used research tool for neurobiologists since the authors' first book on the subject, In Situ Hybridization: Applications to Neurobiology (Oxford, 1987). This new volume examines the methodological advances that have been made in in situhybridization techniques and their impact on the study of the nervous system. These include methods for improving sensitivity, ways of examining multiple mRNAs within the same section, ways of examining both proteins and mRNAs within the same section, and methods for using in situ hybridizationtechnology to examine areas of cellular biological significance, such as translational control. The development of alternative labels to radioactivity for the detection of hybridized probes, and the combined use of in situ hybridization and immunocytochemistry is also covered in this book. Itdiscusses the quantification of in situ hybridization signal and presents an overview of information generated by using in situ hybridization in diagnostic medicine. This volume will be essential to researchers in the neurosciences as well as those in other disciplines.
Molecular Methods for Virus Detection
Danny L. Wiedbrauk,Daniel H. Farkas
Author : Danny L. Wiedbrauk,Daniel H. Farkas
Publisher : Elsevier
Page : 386 pages
File Size : 40,9 Mb
Release : 1995-02-08
Category : Science
ISBN : 0080536891
Molecular Methods for Virus Detection by Danny L. Wiedbrauk,Daniel H. Farkas Pdf
Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods. Written by the inventors or principal developers of these technologies, Molecular Methods for Virus Detection provides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books. Molecular Methods for Virus Detection provides clinically relevant procedures for many of the newer diagnostic methodologies. Provides state-of-the-art PCR methods for amplification, quantitation, in situ hybridization, and multiplex reactions Goes beyond PCR with protocols for 3SR, NASBA, LCR, SDA, and LAT Covers important virus detection methods such as in situ hybridization; Southern, dot, and slot blots; branched chain signal amplification; and chemiluminescence Includes quality control information crucial in research and clinical laboratories Most chapters are written by the inventors and principal developers of the methodologies Includes color plates, 77 figures, and 18 tables
