Pcr Topics

Author : Arndt Rolfs,Hermann C. Schumacher,Peter Marx
Publisher : Springer Science & Business Media
Page : 258 pages
File Size : 48,5 Mb
Release : 2013-12-01
Category : Medical
ISBN : 9783642759246

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PCR Topics by Arndt Rolfs,Hermann C. Schumacher,Peter Marx Pdf

PCR, developed at Cetus Corporation/USA by Henry A. Erlich, Kary Mullis and Randall K. Saiki, is a very simple method for amplifying nucleic acids in vitro. The realization of this idea bases on the repetition of a set of three different temperatures and yields an increase of the target structure up to a factor of 106 to 1012. Therefore, this technique is predisposed for safe analysis and characterization of DNA and RNA sequences of interest, even where the starting amount of material is enormously small. Because of its sensitivity, speed and versatility this method is particularly suitable for investigations of oncogenes, tumor associated translocations, retroviral sequences, lymphokines and mainly the broad field of degenerative and inflammatory diseases of nervous system. PCR seems to be the technique which could overcome the two most important problems in that field: very small amount of material combined with the necessity of rapid diagnostic procedures in inflammatory infections. "PCR topics" will give an actual overview of basic and applied research fields on usage of polymerase chain reaction. All contributions to this book have been presented at an international congress on "Usage of Polymerase chain reaction in genetic and infectious diseases" which took place in june 1990 in Berlin. The editors wish to thank all participants for their contributions. We offer our thanks and gratitude to our coworkers and especially to our technical assistents Barbara Trampenau, Mirjana Wiirdemann and Hannelore Leonhard.

Author : John M. S. Bartlett,David Stirling
Publisher : Springer Science & Business Media
Page : 1083 pages
File Size : 52,9 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592593842

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PCR Protocols by John M. S. Bartlett,David Stirling Pdf

In this new edition, the editors have thoroughly updated and dramatically expanded the number of protocols to take advantage of the newest technologies used in all branches of research and clinical medicine today. These proven methods include real time PCR, SNP analysis, nested PCR, direct PCR, and long range PCR. Among the highlights are chapters on genome profiling by SAGE, differential display and chip technologies, the amplification of whole genome DNA by random degenerate oligonucleotide PCR, and the refinement of PCR methods for the analysis of fragmented DNA from fixed tissues. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle behind the technique, equipment and reagent lists, tips on trouble shooting and avoiding known pitfalls, and, where needed, a discussion of the interpretation and use of results.

Author : Lucília Domingues
Publisher : Springer Nature
Page : 255 pages
File Size : 42,5 Mb
Release : 2023-09-23
Category : Science
ISBN : 9781071633588

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PCR by Lucília Domingues Pdf

This second volume focuses on PCR methods and PCR application specificities to the biotechnology and bioengineering field. New and updated chapters detail real-time PCR protocols, synthetic biology applications, pathogen detection, microfluidics, digital, multiplex detection recent advances. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, PCR: Methods and Protocols, Second Edition aims to be a useful and practical guide to new researchers and experts looking to expand their knowledge.

Author : Kary B. Mullis,Francois Ferre,Richard A. Gibbs
Publisher : Springer Science & Business Media
Page : 464 pages
File Size : 47,9 Mb
Release : 2012-02-02
Category : Medical
ISBN : 9781461202578

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The Polymerase Chain Reaction by Kary B. Mullis,Francois Ferre,Richard A. Gibbs Pdf

James D. Watson When, in late March of 1953, Francis Crick and I came to write the first Nature paper describing the double helical structure of the DNA molecule, Francis had wanted to include a lengthy discussion of the genetic implications of a molecule whose struc ture we had divined from a minimum of experimental data and on theoretical argu ments based on physical principles. But I felt that this might be tempting fate, given that we had not yet seen the detailed evidence from King's College. Nevertheless, we reached a compromise and decided to include a sentence that pointed to the biological significance of the molecule's key feature-the complementary pairing of the bases. "It has not escaped our notice," Francis wrote, "that the specific pairing that we have postulated immediately suggests a possible copying mechanism for the genetic material." By May, when we were writing the second Nature paper, I was more confident that the proposed structure was at the very least substantially correct, so that this second paper contains a discussion of molecular self-duplication using templates or molds. We pointed out that, as a consequence of base pairing, a DNA molecule has two chains that are complementary to each other. Each chain could then act ". . . as a template for the formation on itself of a new companion chain, so that eventually we shall have two pairs of chains, where we only had one before" and, moreover, " ...

Author : Nicola King,Joe O’Connell
Publisher : Springer Science & Business Media
Page : 370 pages
File Size : 52,7 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781592592838

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RT-PCR Protocols by Nicola King,Joe O’Connell Pdf

Until the mid 1980s, the detection and quantification of a specific mRNA was a difficult task, usually only undertaken by a skilled molecular biologist. With the advent of PCR, it became possible to amplify specific mRNA, after first converting the mRNA to cDNA via reverse transcriptase. The arrival of this technique—termed reverse transcription-PCR (RT-PCR)—meant that mRNA suddenly became amenable to rapid and sensitive analysis, without the need for advanced training in molecular biology. This new accessibility of mRNA, which has been facilitated by the rapid accumulation of sequence data for human mRNAs, means that every biomedical researcher can now include measurement of specific mRNA expression as a routine component of his/her research plans. In view of the ubiquity of the use of standard RT-PCR, the main objective of RT-PCR Protocols is essentially to provide novel, useful applications of RT-PCR. These include some useful adaptations and applications that could be relevant to the wider research community who are already familiar with the basic RT-PCR protocol. For example, a variety of different adaptations are described that have been employed to obtain quantitative data from RT-PCR. Quantitative RT-PCR provides the ability to accurately measure changes/imb- ances in specific mRNA expression between normal and diseased tissues.

Author : Anonim
Publisher : BoD – Books on Demand
Page : 336 pages
File Size : 54,8 Mb
Release : 2021-07-21
Category : Medical
ISBN : 9781839684838

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Current Topics and Emerging Issues in Malaria Elimination by Anonim Pdf

Malaria is one of the most important tropical diseases in the history of the world. This vector-borne disease has been a significant cause of morbidity and mortality in tropical countries of Africa, Asia, and Latin America. As such, this book provides updated information on epidemiological and public health research of malaria conducted in the last decade. Over four sections, chapters discuss such topics as diagnosis, epidemiology and surveillance, policy and prevention, and vector control and vaccines.

Author : Y. M. Dennis Lo
Publisher : Humana Press
Page : 0 pages
File Size : 44,7 Mb
Release : 1998-03-02
Category : Science
ISBN : 0896034992

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Clinical Applications of PCR by Y. M. Dennis Lo Pdf

The polymerase chain reaction (PCR) is one of the most important molecular biological methods ever devised, with numerous applications to cli- cal molecular medicine. Since its description in 1985, PCR has undergone tremendous improvements, and many variations on the basic PCR theme have been published. With such a large volume of PCR-related literature, a clinical scientist wishing to use the technique will have a difficult task loc- ing the relevant information to implement it effectively. There is thus clearly a need for an up-to-date volume with detailed protocols to facilitate the setting up of those techniques most relevant to clinical applications. Unlike some other books on this topic, Clinical Applications of PCR includes only methods that are of direct relevance in clinical settings. The book is organized in three parts: an introductory section, a section on general methodology, and a final section with specific clinical applications. The first section covers the basic principles of PCR and is most useful to those new to molecular diagnosis. The next chapter includes useful tips for setting up a PCR laboratory. Section 2 then outlines some of the most commonly used PCR-based techniques in molecular diagnosis. Section 3 includes carefully chosen examples that represent typical applications of PCR in diverse clinical fields, encompassing hematology, oncology, genetics, and microbiology.

Author : Stephen J Meltzer
Publisher : Springer Science & Business Media
Page : 286 pages
File Size : 54,6 Mb
Release : 2008-02-03
Category : Science
ISBN : 9781592595754

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PCR in Bioanalysis by Stephen J Meltzer Pdf

PCR in Bioanalysis offers powerful PCR-based protocols and assays in actual use or potential use in clinical medicine and commercial biology. The main focus of the book is on the commercial applications of PCR, as opposed to basic research uses. Topics covered include the measurement of hormone levels using PCR, transcription factor isolation, detection of viruses using PCR, detection of tumor contamination of stem cells, evaluation of grafts for tumor cells, and more.

Author : Kirstin J. Edwards,Julie M. J. Logan,Nick A. Saunders
Publisher : Taylor & Francis
Page : 362 pages
File Size : 48,8 Mb
Release : 2004
Category : Polymerase chain reaction
ISBN : 9781134184002

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Real-Time PCR by Kirstin J. Edwards,Julie M. J. Logan,Nick A. Saunders Pdf

Author : National Research Council,Division on Earth and Life Studies,Commission on Life Sciences,Committee on DNA Technology in Forensic Science
Publisher : National Academies Press
Page : 199 pages
File Size : 55,6 Mb
Release : 1992-02-01
Category : Science
ISBN : 9780309045872

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DNA Technology in Forensic Science by National Research Council,Division on Earth and Life Studies,Commission on Life Sciences,Committee on DNA Technology in Forensic Science Pdf

Matching DNA samples from crime scenes and suspects is rapidly becoming a key source of evidence for use in our justice system. DNA Technology in Forensic Science offers recommendations for resolving crucial questions that are emerging as DNA typing becomes more widespread. The volume addresses key issues: Quality and reliability in DNA typing, including the introduction of new technologies, problems of standardization, and approaches to certification. DNA typing in the courtroom, including issues of population genetics, levels of understanding among judges and juries, and admissibility. Societal issues, such as privacy of DNA data, storage of samples and data, and the rights of defendants to quality testing technology. Combining this original volume with the new update-The Evaluation of Forensic DNA Evidence-provides the complete, up-to-date picture of this highly important and visible topic. This volume offers important guidance to anyone working with this emerging law enforcement tool: policymakers, specialists in criminal law, forensic scientists, geneticists, researchers, faculty, and students.

Author : Roberto Biassoni,Alessandro Raso
Publisher : Humana
Page : 0 pages
File Size : 41,5 Mb
Release : 2014-04-17
Category : Science
ISBN : 1493907328

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Quantitative Real-Time PCR by Roberto Biassoni,Alessandro Raso Pdf

Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Quantitative Real-Time PCR: Methods and Protocols aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation.

Author : John M. Butler
Publisher : Academic Press
Page : 700 pages
File Size : 54,8 Mb
Release : 2011-07-21
Category : Law
ISBN : 9780123745132

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Advanced Topics in Forensic DNA Typing: Methodology by John M. Butler Pdf

John M. Butler.

Author : Bing-Yuan Chen,Harry W. Janes
Publisher : Springer Science & Business Media
Page : 429 pages
File Size : 53,6 Mb
Release : 2008-02-05
Category : Science
ISBN : 9781592591770

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PCR Cloning Protocols by Bing-Yuan Chen,Harry W. Janes Pdf

PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.

Author : Elizabeth van Pelt-Verkuil,Alex van Belkum,John P. Hays
Publisher : Springer Science & Business Media
Page : 330 pages
File Size : 42,9 Mb
Release : 2008-03-14
Category : Science
ISBN : 9781402062414

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Principles and Technical Aspects of PCR Amplification by Elizabeth van Pelt-Verkuil,Alex van Belkum,John P. Hays Pdf

Kary Mullis was awarded a Nobel Prize for inventing the PCR technique more than a decade ago in 1993. Since its "discovery", multiple adaptations and variations of the standard PCR technique have been described. This publication aims to provide the reader with a guide to the standard PCR technique and its many available variants, with particular emphasis being placed on the role of these PCR techniques in the clinical diagnostic laboratory (the central theme of this book).

Author : Henry Erlich
Publisher : Springer
Page : 246 pages
File Size : 51,5 Mb
Release : 2015-12-31
Category : Science
ISBN : 9781349202355

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PCR Technology by Henry Erlich Pdf

This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.