Physical Principles And Techniques Of Protein Chemistry

Author : Sydney Leach
Publisher : Elsevier
Page : 639 pages
File Size : 46,6 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323141147

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Physical Principles and Techniques of Protein Chemistry Part C by Sydney Leach Pdf

Physical Principles and Techniques of Protein Chemistry, Part C focuses on the effects of intermolecular interactions that are transmitted between ligands and proteins and from protein to protein. This book discusses the density and volume change measurements; direct volume change; osmotic pressure; and small-angle X-ray scattering. The theory of particulate scattering; pulsed nuclear magnetic resonance; absorption of water by diamagnetic molecules; and use of least squares in data analysis are also elaborated. This text likewise covers the iteration process; optical rotatory dispersion and the main chain conformation of proteins; and basic relations for optically active molecules. Other topics include the circular dichroism, secondary structure of proteins, visible rotatory dispersion, and peptide cotton effects. This publication is intended for protein chemists, but is also useful to biologists, medical practitioners, and students researching on protein chemistry.

Author : Anonim
Publisher : Unknown
Page : 621 pages
File Size : 40,6 Mb
Release : 1973
Category : Electronic
ISBN : OCLC:174734645

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Physical Principles and Techniques of Protein Chemistry by Anonim Pdf

Author : Sydney Leach
Publisher : Elsevier
Page : 508 pages
File Size : 47,6 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323141130

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Physical Principles and Techniques of Protein Chemistry Part B by Sydney Leach Pdf

Physical Principles and Techniques of Protein Chemistry, Part B deals with the theories and application of selected physical methods in protein chemistry evaluation. This book is divided into seven chapters that cover the ultracentrifugal analysis, light scattering, infrared (IR) methods, nuclear magnetic resonance (NMR) spectroscopy, and differential thermal analysis of protein properties. This text first describes the fundamental ideas and methodology of sedimentation analysis of ideal noninteracting solutes and the problems of nonideality and solute-solute interaction. This book then deals with the problems involved in the interpretation of viscometric data for evaluation of intrinsic viscosity of proteins. The following chapters examine the principles, measurement and analysis of spectra, and experimental techniques of light scattering, IR, and NMR spectroscopic methods. Discussions on coordination phenomena, identification of binding sites, and ion binding in the crystalline state and in protein solutions are included. The concluding chapter presents some examples of protein analysis using differential thermal analysis technique. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry.

Author : Sydney Leach
Publisher : Elsevier
Page : 545 pages
File Size : 42,5 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323141123

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Physical Principles and Techniques of Protein Chemistry Part A by Sydney Leach Pdf

Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of maps from X-ray methods and the diffraction data from fibrous proteins. The subsequent chapters cover discussions on UV spectroscopy of proteins; luminescence properties of proteins and related compounds; and perturbation and flow methods for evaluation of proteins’ dynamic properties and rate constants. Other chapters deal with the evaluation of proteins’ dielectric properties using dielectric relaxation, electric birefringence, and dichroism techniques. The concluding chapters outline the theoretical and experimental advances of the electrophoretic and gel filtration methods for the study of protein structure and molecular weight. This book is of great value to chemists, biologists, and researchers who have great appreciation of protein chemistry.

Author : S. J. Leach
Publisher : Unknown
Page : 0 pages
File Size : 52,5 Mb
Release : 1970
Category : Electronic
ISBN : OCLC:925991149

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Physical Principles and Techniques of Protein Chemistry by S. J. Leach Pdf

Author : Sydney J. Leach
Publisher : Unknown
Page : 546 pages
File Size : 48,5 Mb
Release : 1969
Category : Science
ISBN : MINN:31951P00706518X

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Physical Principles and Techniques of Protein Chemistry by Sydney J. Leach Pdf

Physical Principles and Techniques of Protein Chemistry, Part A deals with the principles and application of selected physical methods in protein chemistry evaluation. This book is organized into nine chapters that cover microscopic, crystallographic, and electrophoretic techniques for protein conformational perturbations evaluation. This text first presents a general account of electron microscopy, its specimen preparation, optimum conditions for high resolution, measurement of electron micrographs, and illustrative examples of protein study. This book then examines the different types of map ...

Author : G.E. Schulz,R.H. Schirmer
Publisher : Springer Science & Business Media
Page : 328 pages
File Size : 48,8 Mb
Release : 2013-12-01
Category : Science
ISBN : 9781461261377

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Principles of Protein Structure by G.E. Schulz,R.H. Schirmer Pdf

New textbooks at all levels of chemistry appear with great regularity. Some fields like basic biochemistry, organic reaction mechanisms, and chemical thermodynamics are well represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a real lack of up-to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research which is advancing the field. It is not often easy to persuade such individuals to set time aside to help spread the knowledge they have accumulated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instructive introductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In some cases the availability of texts in active research areas should help stimulate the creation of new courses.

Author : Jack Kyte
Publisher : Garland Science
Page : 1505 pages
File Size : 54,7 Mb
Release : 2006-11-01
Category : Science
ISBN : 9781136843501

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Structure in Protein Chemistry by Jack Kyte Pdf

The second edition of Structure in Protein Chemistry showcases the latest developments and innovations in the field of protein structure analysis and prediction. The book begins by explaining how proteins are purified and describes methods for elucidating their sequences of amino acids and defining their posttranslational modifications. Comprehensive explanations of crystallography and of noncovalent forces-ionic interactions, hydrogen bonding, and the hydrophobic effect-act as a prelude to an exhaustive description of the atomic details of the structures of proteins. The resulting understanding of protein molecular structure forms the basis for discussions of the evolution of proteins, the symmetry of the oligomeric associations that produce them, and the chemical, mathematical, and physical basis of the techniques used to study their structures. The latter include image reconstruction, nuclear magnetic resonance spectroscopy, proton exchange, optical spectroscopy, electrophoresis, covalent cross-linking, chemical modification, immunochemistry, hydrodynamics, and the scattering of light, X-radiation, and neutrons. These procedures are applied to study the folding of polypeptides and the assembly of oligomers. Biological membranes and their proteins are also discussed. Structure in Protein Chemistry, Second Edition, bridges the gap between introductory biophysical chemistry courses and research literature. It serves as a comprehensive textbook for advanced undergraduates and graduate students in biochemistry, biophysics, and structural and molecular biology. Professionals engaged in chemical, biochemical, and molecular biological research will find it a useful reference.

Author : Robert K. Scopes
Publisher : Springer Science & Business Media
Page : 342 pages
File Size : 48,6 Mb
Release : 2013-04-17
Category : Science
ISBN : 9781475719574

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Protein Purification by Robert K. Scopes Pdf

New textbooks at alllevels of chemistry appear with great regularity. Some fields like basic biochemistry, organic reaction mechanisms, and chemical thermodynamics are weil represented by many excellent texts, and new or revised editions are published sufficiently often to keep up with progress in research. However, some areas of chemistry, especially many of those taught at the graduate level, suffer from a reallack ofup-to-date textbooks. The most serious needs occur in fields that are rapidly changing. Textbooks in these subjects usually have to be written by scientists actually involved in the research which is advancing the field. lt is not often easy to persuade such individuals to settime aside to help spread the knowledge they have accumu lated. Our goal, in this series, is to pinpoint areas of chemistry where recent progress has outpaced what is covered in any available textbooks, and then seek out and persuade experts in these fields to produce relatively concise but instructive introductions to their fields. These should serve the needs of one semester or one quarter graduate courses in chemistry and biochemistry. In some cases the availability of texts in active research areas should help stimulate the creation of new courses. NewYork CHARLES R. CANTOR Preface to the Second Edition The original plan for the first edition of this book was to title it Enzyme Purification: Princip/es and Practice.

Author : Jan-Christer Janson
Publisher : John Wiley & Sons
Page : 542 pages
File Size : 54,5 Mb
Release : 2012-01-03
Category : Science
ISBN : 9781118002193

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Protein Purification by Jan-Christer Janson Pdf

The authoritative guide on protein purification—now completely updated and revised Since the Second Edition of Protein Purification was published in 1998, the sequencing of the human genome and other developments in bioscience have dramatically changed the landscape of protein research. This new edition addresses these developments, featuring a wealth of new topics and several chapters rewritten from scratch. Leading experts in the field cover all major biochemical separation methods for proteins in use today, providing professionals in biochemistry, organic chemistry, and analytical chemistry with quick access to the latest techniques. Entirely new or thoroughly revised content includes: High-resolution reversed-phase liquid chromatography Electrophoresis in gels Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients Affinity ligands from chemical and biological combinatorial libraries Membrane separations Refolding of inclusion body proteins from E. coli Purification of PEGylated proteins High throughput screening techniques in protein purification The history of protein chromatography

Author : Joe Regenstein
Publisher : Elsevier
Page : 368 pages
File Size : 51,6 Mb
Release : 2012-12-02
Category : Technology & Engineering
ISBN : 9780323153867

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Food Protein Chemistry by Joe Regenstein Pdf

Food Protein Chemistry: An Introduction for Food Scientists discusses food proteins and how they are studied. Proteins are both biological entities and physicochemical compounds, and they will be examined in both contexts in this volume. The chemical and physical properties of proteins will be viewed from the perspective of chemists despite the fact that their use in the food supply emphasizes their biological nature. Key topics discussed include proteins as essential to life; amino acids; protein classification; selected proteins of the most important food systems; and protein structure. The book also includes chapters on protein measurement; protein purification; and spectral techniques for the study of proteins. The book requires readers to have the equivalent of the Institute of Food Technologists requirements for undergraduate food science majors. It also assumes a knowledge of math through calculus. While primarily intended for senior and first-year graduate food science students, the text may also be useful to researchers in allied fields.

Author : Engelbert Buxbaum
Publisher : Springer Science & Business Media
Page : 490 pages
File Size : 47,9 Mb
Release : 2010-11-18
Category : Science
ISBN : 9781441972514

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Biophysical Chemistry of Proteins by Engelbert Buxbaum Pdf

The book is structured in nine sections, each containing several chapters. The volume starts with an overview of analytical techniques and progresses through purification of proteins; protein modification and inactivation; protein size, shape, and structure; enzyme kinetics; protein-ligand interactions; industrial enzymology; and laboratory quality control. The book is targeted at all scientists interested in protein research.

Author : Bruce Alberts
Publisher : Unknown
Page : 0 pages
File Size : 55,6 Mb
Release : 2002
Category : Cytology
ISBN : 0815332181

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Molecular Biology of The Cell by Bruce Alberts Pdf

Author : Eugene A. Permyakov
Publisher : CRC Press
Page : 172 pages
File Size : 46,9 Mb
Release : 2018-01-18
Category : Medical
ISBN : 9781351082662

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Luminescent Spectroscopy of Proteins by Eugene A. Permyakov Pdf

Luminescent Spectroscopy of Proteins is devoted to the method of intrinsic protein luminescence, one of the most popular experimental methods in modern biophysics and biochemistry. The book discusses general physical principles of the luminescence method; spectral properties of the main protein chromophores; and protein luminescence and its use for studies on structural, physico-chemical, and functional properties of proteins. Principles of luminescent spectroscopy are illustrated by real-life applications and problems. Luminescent Spectroscopy of Proteins will be an excellent reference for biophysicists, biochemists, analytical chemists, and other scientists interested in this topic.

Author : David Glick
Publisher : John Wiley & Sons
Page : 395 pages
File Size : 51,5 Mb
Release : 2009-09-25
Category : Science
ISBN : 9780470110812

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Methods of Biochemical Analysis by David Glick Pdf

An encyclopedia of methods in biochemical analysis, this modern series keeps biochemists and analytical chemists abreast of experimental innovations and improvements in biochemical techniques and instrumentation.