Plant Genome Editing With Crispr Systems

Author : Yiping Qi
Publisher : Springer Nature
Page : 360 pages
File Size : 41,6 Mb
Release : 2019-01-04
Category : Science
ISBN : 9781493989911

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Plant Genome Editing with CRISPR Systems by Yiping Qi Pdf

This volume provides readers with wide-ranging coverage of CRISPR systems and their applications in various plant species. The chapters in this book discuss topics such as plant DNA repair and genome editing; analysis of CRISPR-induced mutations; multiplexed CRISPR/Cas9 systems; CRISPR-Cas12a (Cpf1) editing systems; and non-agrobacterium based CRISPR delivery systems. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and thorough, Plant Genome Editing with CRISPR Systems: Methods and Protocols is a valuable resource for any researcher interested in learning about and using CRISPR systems in plants.

Author : Deepu Pandita,Anu Pandita
Publisher : CRC Press
Page : 463 pages
File Size : 55,5 Mb
Release : 2023-06-30
Category : Science
ISBN : 9781000771251

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CRISPR/Cas-Mediated Genome Editing in Plants by Deepu Pandita,Anu Pandita Pdf

With over 820 million people facing hunger in today’s world, the need of the hour is the design of plant varieties with high yield, improved traits, and resistance properties in order to mitigate the detrimental impacts of biotic and abiotic stress conditions on food crops. This volume highlights the use of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR-associated nuclease proteins as a solution for sustainable agriculture. CRISPR/Cas-mediated genome editing (GE) provides a robust, simple, precise, efficient, economical, and revolutionary toolbox that leads to improved plant traits with enhanced yield, quality, and resistance against various stresses, including climate-related stress, insect pests and diseases, pollution-related stresses, etc. Key features of the book: Explains the differences between conventional breeding, genetically modified crops, and genome editing approaches using CRISPR/Cas Introduces the tools of genome editing, such as ZFNS (zinc finger nucleases), mega nucleases, and TLENS (transcription activator-like effector nucleases) Covers the history, origin, discovery, structure, and classification of CRISPR/Cas Examines the databases and computational approaches of CRISPR/Cas Highlights genome editing of important crop plants with CRISPR/Cas systems, including wheat, maize. tomato, Brassica crops, rice, fruits Explores potential applications of CRISPR/Cas systems for climate smart crops

Author : Ashwani Kumar
Publisher : Springer Nature
Page : 871 pages
File Size : 49,5 Mb
Release : 2024-06-02
Category : Electronic
ISBN : 9789819985296

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Gene Editing in Plants by Ashwani Kumar Pdf

Author : Anshu Alok,Jitesh Kumar,Mahipal Shekhawat
Publisher : CRC Press
Page : 329 pages
File Size : 48,7 Mb
Release : 2024-08-23
Category : Science
ISBN : 9781040112137

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Basics of CRISPR/Cas Mediated Plant Genome Editing by Anshu Alok,Jitesh Kumar,Mahipal Shekhawat Pdf

CRISPR/Cas has emerged, as a powerful genetic engineering tool. It is simple yet highly affordable and robust. This book provides a basic introduction to the mechanism and architecture of the CRISPR/Cas. It enlightens the readers about the new advancements such as gene insertion, transcriptional activation, suppression, gene tagging, multiplexing, base editing, prime editing, and tissue culture free editing which makes it easier for crop improvement. With such technologies, application of genome editing has opened new paths for crop improvements and sustainable agriculture. The current book will be useful for beginners to understand these tools and how to use them in a better and more efficient way.

Author : Om Prakash Gupta,Suhas Gorakh Karkute
Publisher : CRC Press
Page : 382 pages
File Size : 45,5 Mb
Release : 2021-07-25
Category : Science
ISBN : 9781000410198

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Genome Editing in Plants by Om Prakash Gupta,Suhas Gorakh Karkute Pdf

Genome Editing in Plants: Principles and Applications addresses the information of genome editing starting from principles and historical aspects to the latest advancements in the field. As genome-editing technology has emerged as promising and cutting edge, researchers around the world have started producing original research outputs, which have significantly improved our current understanding and potential of this technology. The initial chapters of this book describe different genome-editing tools as well as their principles and applications. Other chapters are dedicated to the present status and future applications of genome-editing techniques in various crop improvement programmes. Some of the advanced applications of CRISPR/Cas tools, such as base editing and RNA detection, along with regulatory aspects of genome-edited crops are described in detail. This book serves as a valuable resource to researchers in the field of crop improvement; graduate and postgraduate students engaged in plant molecular biology and biotechnology; academicians; and policy makers. Key Features: Addresses topics associated with historical development and principles of genome-editing technology Addresses basic mechanisms operating under each genome-editing technology Addresses its application in plants to design crops as per the current and future demands Addresses the regulatory mechanisms of genome-edited crops

Author : Vijai Singh,Pawan K. Dhar
Publisher : Academic Press
Page : 0 pages
File Size : 50,9 Mb
Release : 2020-02-21
Category : Science
ISBN : 0128181400

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Genome Engineering via CRISPR-Cas9 System by Vijai Singh,Pawan K. Dhar Pdf

Genome Engineering via CRISPR-Cas9 Systems presents a compilation of chapters from eminent scientists from across the globe who have established expertise in working with CRISPR-Cas9 systems. Currently, targeted genome engineering is a key technology for basic science, biomedical and industrial applications due to the relative simplicity to which they can be designed, used and applied. However, it is not easy to find relevant information gathered in a single source. The book contains a wide range of applications of CRISPR in research of bacteria, virus, algae, plant and mammalian and also discusses the modeling of drosophila, zebra fish and protozoan, among others. Other topics covered include diagnosis, sensor and therapeutic applications, as well as ethical and regulatory issues. This book is a valuable source not only for beginners in genome engineering, but also researchers, clinicians, stakeholders, policy makers, and practitioners interested in the potential of CRISPR-Cas9 in several fields.

Author : Bing Yang,Wendy Harwood,Qiudeng Que
Publisher : Springer Nature
Page : 382 pages
File Size : 51,9 Mb
Release : 2023-03-30
Category : Science
ISBN : 9781071631317

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Plant Genome Engineering by Bing Yang,Wendy Harwood,Qiudeng Que Pdf

This volume provides readers with a collection of the latest protocols used to study plant genome editing and trait engineering. The chapters in this book are organized into five parts: genome engineering systems; machinery design and validation; delivery tools; generation and analysis of engineering materials; and crop genome engineering applications. The chapters cover topics such as methods of applying the popular CRISPR-Cas9 or CRISPR-Cas12 systems for editing genomes in different crop species, the use of small synthetic plastome for potato genome engineering, and the use of CRISPR-Cas9 for algal cell genome engineering. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Plant Genome Engineering: Methods and Protocols is a valuable tool for researchers interested in learning more about this developing and important field.

Author : Bing Yang,Jimmy R. Botella
Publisher : Frontiers Media SA
Page : 94 pages
File Size : 55,8 Mb
Release : 2022-10-26
Category : Science
ISBN : 9782832503355

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Insights in Genome Editing in Plants: 2021 by Bing Yang,Jimmy R. Botella Pdf

Author : Kamel A Abd-Elsalam,Ki-Taek Lim
Publisher : Elsevier
Page : 842 pages
File Size : 55,6 Mb
Release : 2021-02-27
Category : Technology & Engineering
ISBN : 9780128219119

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CRISPR and RNAi Systems by Kamel A Abd-Elsalam,Ki-Taek Lim Pdf

Plants are vulnerable to pathogens including fungi, bacteria, and viruses, which cause critical problems and deficits. Crop protection by plant breeding delivers a promising solution with no obvious effect on human health or the local ecosystem. Crop improvement has been the most powerful approach for producing unique crop cultivars since domestication occurred, making possible the main innovations in feeding the globe and community development. Genome editing is one of the genetic devices that can be implemented, and disease resistance is frequently cited as the most encouraging application of CRISPR/Cas9 technology in agriculture. Nanobiotechnology has harnessed the power of genome editing to develop agricultural crops. Nanosized DNA or RNA nanotechnology approaches could contribute to raising the stability and performance of CRISPR guide RNAs. This book brings together the latest research in these areas. CRISPR and RNAi Systems: Nanobiotechnology Approaches to Plant Breeding and Protection presents a complete understanding of the RNAi and CRISPR/Cas9 techniques for controlling mycotoxins, fighting plant nematodes, and detecting plant pathogens. CRISPR/Cas genome editing enables efficient targeted modification in most crops, thus promising to accelerate crop improvement. CRISPR/Cas9 can be used for management of plant insects, and various plant pathogens. The book is an important reference source for both plant scientists and environmental scientists who want to understand how nano biotechnologically based approaches are being used to create more efficient plant protection and plant breeding systems. Shows how nanotechnology is being used as the basis for new solutions for more efficient plant breeding and plant protection Outlines the major techniques and applications of both CRISPR and RNAi technologies Assesses the major challenges of escalating these technologies on a mass scale

Author : Ashwani Kumar,Sudipti Arora,Shinjiro Ogita,Yuan-Yeu Yau,Krishnendu Mukherjee
Publisher : Springer
Page : 0 pages
File Size : 40,9 Mb
Release : 2024-02-20
Category : Science
ISBN : 9819985285

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Gene Editing in Plants by Ashwani Kumar,Sudipti Arora,Shinjiro Ogita,Yuan-Yeu Yau,Krishnendu Mukherjee Pdf

This book is a collection of information about applying CRISPR-Cas systems for genome editing in plants. The main focus of this book is to address the recent advances and future prospects of CRISPR-Cas technology in crops. Genome editing technology is important because it can be used to improve plant traits. The earlier genome-engineering tools, zinc finger nucleases (ZFNs), and TAL effector nucleases (TALENs) are complicated to design and not flexible. The novel genome editor, CRISPR-Cas systems, has advantages over ZFNs and TALENs. The advantages are simple and easy to design precision in targeting and efficiency. Due to its precision and simplicity, the CRISPR-Cas technology has rapidly become the most popular genome-editing platform in life-science fields. CRISPR-Cas technology has been used widely for human gene therapy to treat diseases and for plant breeding programs for crop improvement. This book is of interest and useful to genome-editing professionals, plant breeders, horticulturists, field-level extension workers, nurserymen, planters, ecologists, and valuable source of reference to the relevant researchers.

Author : Shabir Hussain Wani,Goetz Hensel
Publisher : Springer Nature
Page : 338 pages
File Size : 48,8 Mb
Release : 2022-11-08
Category : Science
ISBN : 9783031080722

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Genome Editing by Shabir Hussain Wani,Goetz Hensel Pdf

Over the last few decades, various techniques have been developed to alter the properties of plants and animals. While the targeted transfer of recombinant DNA into crop plants remains a valuable tool to achieve a desirable breeding outcome, integration of transgenes into the host genome has been random, which in part, leads to reduced acceptance of GMOs by the general population in some parts of the world. Likewise, methods of induced mutagenesis, such as TILLING, have the disadvantage that many mutations are induced per plant, which has to be removed again by expensive backcrossing. Advances in genome sequencing have provided more and more information on differences between susceptible and resistant varieties, which can now be directly targeted and modified using CRISPR/Cas9 technology. By selecting specific gRNAs occurrence of off-target modifications are comparatively low. ZFNs and TALENs- based approaches required re-engineering a new set of assembled polypeptides for every new target site for each experiment. The difficulty in cloning and protein engineering prevented these tools from being broadly adopted by the scientific community. Compared to these technologies, designing the CRISPR toolbox is much simpler and more flexible. CRISPR/Cas9 is versatile, less expensive and highly efficient. It has become the most widely used technology for genome editing in many organisms. Since its inception as a powerful genome-editing tool in late 2012, this breakthrough technology has completely changed how science is performed. The first few chapters in this book introduce the basic concept, design and implementation of CRISPR/Cas9 for different plant systems. They are followed by in-depth discussions on the legal and bio-safety issues accompanying commercialization and patenting of this emerging technology. Lastly, this book covers emerging areas of new tools and potential applications. We believe readers, novice and expert alike, will benefit from this all-in-one resource on genome editing for crop improvement. Chapter 17 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Author : Anjanabha Bhattacharya,Vilas Parkhi,Bharat Char
Publisher : Springer Nature
Page : 232 pages
File Size : 40,7 Mb
Release : 2020-12-11
Category : Science
ISBN : 9783030420222

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CRISPR/Cas Genome Editing by Anjanabha Bhattacharya,Vilas Parkhi,Bharat Char Pdf

This book offers a comprehensive collection of papers on CRISPR/Cas genome editing in connection with agriculture, climate-smart crops, food security, translational research applications, bioinformatics analysis, practical applications in cereals, floriculture crops, engineering plants for abiotic stress resistance, the intellectual landscape, regulatory framework, and policy decisions. Gathering contributions by internationally respected experts in the field of CRISPR/Cas genome editing, the book offers an essential guide for researchers, students, teachers and scientists in academia; policymakers; and public companies, private companies and cooperatives interested in understanding and/or applying CRISPR/Cas genome editing to develop new agricultural products.

Author : Agnès Ricroch,Dennis Eriksson,Dragana Miladinović,Jeremy Sweet,Katrijn Van Laere,Ewa Woźniak-Gientka
Publisher : Springer Nature
Page : 546 pages
File Size : 54,6 Mb
Release : 2024-01-15
Category : Science
ISBN : 9783031461507

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A Roadmap for Plant Genome Editing by Agnès Ricroch,Dennis Eriksson,Dragana Miladinović,Jeremy Sweet,Katrijn Van Laere,Ewa Woźniak-Gientka Pdf

This open access book is an update of genome editing techniques applied to a range of plants. We discuss the latest techniques and applications to cereals, roots and tubers, oilseed crops, fruit and forest trees, vegetables, legumes and algae including resistance to biotic and abiotic stresses, improved quality, drug production, yield and adaptation to climate change. The regulations in different countries worldwide, the patentibility and the perception by society of the applications of new genomic techniques are examined. This book is written by a multidisciplinary and multisectoral collective of high-profile scientists and other experts belonging to the COST Action network PlantEd, which is mainly European but with contributions from American, Australian, Canadian, Chinese, Indian, Iranian, Pakistani and Peruvian scientists. The book is aimed at a wide audience consisting of students, academics, private and public breeders, other actors in the food and bioeconomy value chains and policy and law makers. This is an open access book.

Author : Anonim
Publisher : Academic Press
Page : 264 pages
File Size : 46,8 Mb
Release : 2017-08-31
Category : Science
ISBN : 9780128117446

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Gene Editing in Plants by Anonim Pdf

Gene Editing in Plants, Volume 149 aims to provide the reader with an up-to-date survey of cutting-edge research with gene editing tools and an overview of the implications of this research on the nutritional quality of fruits, vegetables and grains. New chapters in the updated volume include topics relating to Genome Engineering and Agriculture: Opportunities and Challenges, the Use of CRISPR/Cas9 for Crop Improvement in Maize and Soybean, the Use of Zinc-Finger Nucleases for Crop Improvement, Gene Editing in Polyploid Crops: Wheat, Camelina, Canola, Potato, Cotton, Peanut, Sugar Cane, and Citrus, and Gene Editing With TALEN and CRISPR/Cas in Rice. This ongoing serial contain contributions from leading scientists and researchers in the field of gene editing in plants who describe the results of their own research in this rapidly expanding area of science. Shows the importance of revolutionary gene editing technology on plant biology research and its application to agricultural production Provides insight into what may lie ahead in this rapidly expanding area of plant research and development Contains contributions from major leaders in the field of plant gene editing

Author : Jen-Tsung Chen
Publisher : CRC Press
Page : 389 pages
File Size : 52,9 Mb
Release : 2024-05-08
Category : Science
ISBN : 9781040015155

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CRISPR and Plant Functional Genomics by Jen-Tsung Chen Pdf

CRISPR is a crucial technology in plant physiology and molecular biology resulting in more sustainable agricultural practices, including outcomes of better plant stress tolerance and crop improvement. CRISPR and Plant Functional Genomics explores ways to release the potential of plant functional genomics, one of the prevailing topics in plant biology and a critical technology for speed and precision crop breeding. This book presents achievements in plant functional genomics and features information on diverse applications using the emerging CRISPR-based genome editing technologies producing high-yield, disease-resistant, and climate-smart crops. It also includes theories on organizing strategies for upgrading the CRISPR system to increase efficiency, avoid off-target effects, and produce transgene-free edited crops. Features: Presents CRISPR-based technologies, releasing the potential of plant functional genomics Provides methods and applications of CRISPR/Cas-based plant genome editing technologies Summarizes achievements of speed and precision crop breeding using CRISPR-based technologies Illustrates strategies to upgrade the CRISPR system Supports the UN’s sustainable development goals to develop future climate-resilient crops CRISPR and Plant Functional Genomics provides extensive knowledge of CRISPR-based technologies and plant functional genomics, and is an ideal reference for researchers, graduate students, and practitioners in the field of plant sciences as well as agronomy and agriculture.