Download Full eBooks in PDF
Plant Molecular Biology A Laboratory Manual Book in PDF, ePub and Kindle version is available to download in english. Read online anytime anywhere directly from your device. Click on the download button below to get a free pdf file of Plant Molecular Biology A Laboratory Manual book. This book definitely worth reading, it is an incredibly well-written.
Author : M. Clark
Publisher : Unknown
Page : 128 pages
File Size : 44,6 Mb
Release : 2024-07-01
Category : Electronic
ISBN : 0387584056
Author : Melody S. Clark
Publisher : Springer Science & Business Media
Page : 551 pages
File Size : 55,8 Mb
Release : 2013-11-27
Category : Science
ISBN : 9783642878732
Covering the whole range of molecular biology techniques - genetic engineering as well as cytogenetics of plants -, each chapter begins with an introduction to the basic approach. followed by detailed methods with easy-to-follow protocols and comprehensive troubleshooting. The first part introduces basic molecular methodology such as DNA extraction, blotting, production of libraries and RNA cloning, while the second part describes analytical approaches, in particular RAPD and RFLP. The manual concludes with a variety of gene transfer techniques and both molecular and cytological analysis. As such, this will be of great use to both the first-timer and the experienced scientist.
Author : M.S. Punia
Publisher : Scientific Publishers
Page : 255 pages
File Size : 48,5 Mb
Release : 2018-01-01
Category : Technology & Engineering
ISBN : 9789388172363
The book, “A Laboratory Manual of Plant Biotechnology and Molecular Biology” comprises of workable laboratory protocols for a large number of techniques related to plant biotechnology, genetic engineering and molecular biology. This includes plant cell and tissue culture, callus and suspension culture, anther culture, ovule culture, embryo culture, Cryopreservation, Isolation of Plant protoplasts, Protoplast culture and regeneration, production of somatic hybrids through protoplast fusion, gene transformation using Agrobacterium as vector, direct gene transfer using biolistic gun, Isolation of plant and organells DNA, construction and screening of genomic DNA libraries, Molecular markers like RFLP, RAPD, SCARS and CAPS, DNA sequencing, RNA isolation and northern blotting, Isolation of proteins and western blotting etc. The manual is prepared with the objective to cater the needs of post- graduate students as well as for scientists working in the disciplines of Plant Breeding, Genetics, Botany, Plant physiology, Biochemistry, Plant Biotechnology, Molecular Biology etc. It gives an update on some well established methods and presents reliable protocols.
Author : S. B. Gelvin
Publisher : Springer
Page : 111 pages
File Size : 51,7 Mb
Release : 2013-06-29
Category : Science
ISBN : 9789401748209
During the past ten years, great advances have been made in the area of plant molecular biology. Such formerly esoteric techniques as gene transfer and plant regeneration are now routinely performed, making the dissection of regulatory elements of genes a common practice in many laboratories. Along with this new technology has come an almost bewildering array of rapidly changing techniques, often making it difficult for the novice to select and perform the technique most appropriate for answering a given biological question. In 1986, some of us felt that many of these techniques had become routine enough to warrant the publication of a laboratory manual. The manual is designed both for advanced college level laboratory courses and as a 'bench guide' for use in the scientific laboratory. Recognizing the rapidly changing nature of plant molecular biology technology, the editors have designed a laboratory manual that is both easy to use in the laboratory and which will be updated as the techniques change and new technologies are devised. Additional chapters that can replace or be added to this first edition will be published periodically. The editors recognize that many of the techniques described in this manual depend upon specialized plant genetic material, microbial strains, or recombinant plasmids. Those people desiring such material should contact the relevant authors directly. A list of the various contributors to this manual, including their addresses, is included.
Author : S.B. Gelvin,R.A. Schilperoort
Publisher : Springer Science & Business Media
Page : 180 pages
File Size : 41,6 Mb
Release : 2013-03-09
Category : Science
ISBN : 9789401142175
Plant Molecular Biology Manual (Second Edition) is an entirely new manual containing both fundamental and recently described techniques in the area of plant molecular biology. Designed for use in the research laboratory, the Plant Molecular Biology Manual presents detailed techniques in the areas of plant transformation, recombinant DNA and other nucleic acid manipulations, nuclear run-on and in vitro transcription systems, in situ hybridization and immunodetection systems, protein-nucleic acid interaction analyses, subcellular targeting of proteins in the plant cell, and gene tagging using T-DNA and transposons. This second edition contains more than 40 newly written chapters, including descriptions of subjects such as virus-mediated gene transfer, specialized Agrobacterium strains and T-DNA vectors, nuclear run-on and in vitro transcription systems, non-radioactive detection systems, characterization of transcription factors, nuclear protein targeting, and T-DNA and transposon mutagenesis, not previously described in the first edition.
Author : Sue Carson,Heather Miller,Melissa C. Srougi,D. Scott Witherow
Publisher : Academic Press
Page : 294 pages
File Size : 54,6 Mb
Release : 2019-03-05
Category : Science
ISBN : 9780128157756
Molecular Biology Techniques: A Classroom Laboratory Manual, Fourth Edition is a must-have collection of methods and procedures on how to create a single, continuous, comprehensive project that teaches students basic molecular techniques. It is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology—or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students will gain hands-on experience on subcloning a gene into an expression vector straight through to the purification of the recombinant protein. Presents student-tested labs proven successful in real classroom laboratories Includes a test bank on a companion website for additional testing and practice Provides exercises that simulate a cloning project that would be performed in a real research lab Includes a prep-list appendix that contains necessary recipes and catalog numbers, providing staff with detailed instructions
Author : USA (Ed. ). Gelvin, S. B., Purdue University, West Lafayette, IN
Publisher : Springer
Page : 105 pages
File Size : 49,6 Mb
Release : 1993-01-01
Category : Science
ISBN : 0792322010
A comprehensive manual dealing with the molecular aspects of plant molecular biology, which is designed both as a teaching aid for advanced laboratory courses and as a guide for laboratory research. Subjects covered include the introduction of genes into plants, assays of gene activity, plant nucleic acid extraction and characterization procedures, and the use of selectable and screenable markers in transgenic plants. Special emphasis is placed on the use of Agrobacterium tumefaciens to introduce genes into plants, but other gene introduction techniques are also presented. Each chapter, written by experts in the field, includes a discussion of the background to each technique, detailed protocols, media and solution formulas, and an extensive bibliography.
Author : Heather Miller,D. Scott Witherow,Sue Carson
Publisher : Academic Press
Page : 232 pages
File Size : 46,7 Mb
Release : 2011-10-18
Category : Science
ISBN : 9780123855459
This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Author : P. Maliga
Publisher : Unknown
Page : 478 pages
File Size : 52,6 Mb
Release : 1995
Category : Science
ISBN : UOM:39015053939826
Author : Russell Malmberg,Joachim W. Messing,Ian M. Sussex
Publisher : CSHL Press
Page : 162 pages
File Size : 42,6 Mb
Release : 1985
Category : Nature
ISBN : 0879691840
Author : James D. Mauseth
Publisher : Jones & Bartlett Publishers
Page : 829 pages
File Size : 47,5 Mb
Release : 2016-07-06
Category : Medical
ISBN : 9781284077537
The Sixth Edition of Botany: An Introduction to Plant Biology provides a modern and comprehensive overview of the fundamentals of botany while retaining the important focus of natural selection, analysis of botanical phenomena, and diversity.
Author : Ghislain, M. ,Zhang, D.P.; ,Herrera, M.R.
Publisher : International Potato Center
Page : 52 pages
File Size : 42,8 Mb
Release : 2024-07-01
Category : Electronic
ISBN : 8210379456XXX
Author : R. Malmberg
Publisher : Unknown
Page : 0 pages
File Size : 40,7 Mb
Release : 1985
Category : Electronic
ISBN : OCLC:708367284
Author : Mary A. Schuler,Raymond E. Zielinski
Publisher : Academic Press
Page : 188 pages
File Size : 41,7 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323137652
Methods in Plant Molecular Biology is a lab manual that introduces students to a diversity of molecular techniques needed for experiments with plant cells. Those included have been perfected and are now presented for the first time in a usable and teachable form. Because the manual integrates protein, RNA, and DNA techniques, it will serve students, teachers, and researchers in plant physiology, biophysics, and animal molecular biology who have no previous experience handling recombinant DNA or purified proteins. It can also be used by the established molecular biologist who wishes to utilize the powerful techniques of recombinant DNA to explore the mysteries of the plant kingdom. Eight basic experiments which can be used collectively or individually cover Recombinant Cloning and Screening in E. coli; DNA Sequencing Plant RNA Isolation and in Vitro Translations Plant DNA Isolations and Genomic DNA Southern AnalysisChloroplast Isolation and Protein SynthesisPlant Tissue Culture and Agrobacterium Transformations Experiments that have been student tested for three years Blueprints for setting up gel rigs Comprehensive course schedule outlining individual procedures to be finished in each lab segment Course can be tailored to suit the needs of the individual instructor
Author : S. K. Gakhar,Monika Miglani,Ashwani Kumar
Publisher : Unknown
Page : 0 pages
File Size : 53,9 Mb
Release : 2013-09-30
Category : Electronic
ISBN : 9382332308
The objective of this text is to train young teachers from colleges and research institutions so that they can advance their research in various fields of biology. It will also help students at BSc and MSc level to learn the techniques involved in molecular biology. The book contains four chapters providing step-by-step protocols. In addition, it has general instructions for safety procedures.