Platelet Protocols

Author : Melanie McCabe White,Lisa K. Jennings
Publisher : Elsevier
Page : 131 pages
File Size : 45,9 Mb
Release : 1999-04-21
Category : Science
ISBN : 9780080539126

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Platelet Protocols by Melanie McCabe White,Lisa K. Jennings Pdf

Platelets are fragments of blood cells that occur in the blood of vertebrates and are associated with blood clotting. Scientists have made great strides in recent years in understanding what stimulates platelets to form blood clots at the molecular level and in developing drugs to inhibit platelet action. Their work has a direct effect on millions of people who deal with cardiovascular disease, strokes, surgery, physical trauma, and other conditions. While references to platelet function have been included in some large texts, there has not been a basic reference manual that researchers and clinicians can use in their daily work until now. Platelet Protocols fills the need for a straightforward and comprehensive laboratory manual on current procedures for evaluating and analyzing platelet function and abnormalities. It is an easy-to-read, understandable resource which can be kept at the bench and referred to frequently by scientists, clinicians, and laboratory staff involved in platelet related areas. Topics range from the basics of anticoagulants to the latest developments in platelet testing. Includes: A basic introduction to platelet anatomy and physiology Testing procedures for new anti-platelet therapies Descriptions of platelet function abnormalities Therapeutic approaches to inhibition of platelet function Step-by-step methodologies with clear explanations Helpful appendixes of recipes, instructions, sources of reagents, and more

Author : Jonathan M. Gibbins,Martyn P. Mahaut-Smith
Publisher : Springer Science & Business Media
Page : 389 pages
File Size : 44,7 Mb
Release : 2008-02-04
Category : Medical
ISBN : 9781592597826

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Platelets and Megakaryocytes by Jonathan M. Gibbins,Martyn P. Mahaut-Smith Pdf

12 The average human body has in the order of 10 circulating platelets. They are crucial for hemostasis, and yet excessive platelet activation is a major cause of m- bidity and mortality in western societies. It is therefore not surprising that platelets have become one of the most extensively investigated biological cell types. We are, however, far from understanding precisely how platelets become activated under physiological and pathophysiological conditions. In addition, there are large gaps in our knowledge of platelet production from their giant precursor cell, the megakar- cyte. Understanding megakaryocyte biology will be crucial for the development of platelet gene targeting. The aim of Platelets and Megakaryocytes is therefore to bring together established and recently developed techniques to provide a comprehensive guide to the study of both the platelet and the megakaryocyte. It consists of five s- tions split between two volumes. The more functional assays appear in Volume 1, whereas Volume 2 includes signaling techniques, postgenomic methods, and a n- ber of key perspectives chapters. Part I of Volume 1, Platelets and Megakaryocytes: Functional Assays, describes many well established approaches to the study of platelet function, including aggregometry, secretion, arachidonic acid metabolism, procoagulant responses, pla- let adhesion under static or flow conditions, flow cytometry, and production of microparticles. Although one would ideally wish to perform experiments with human platelets, studies within the circulation using intravital microscopy require the use of animal models, which are described in Chapter 16, vol. 1.

Author : Jonathan M. Gibbins,Martyn Mahaut-Smith
Publisher : Humana Press
Page : 287 pages
File Size : 51,7 Mb
Release : 2018-08-29
Category : Science
ISBN : 1493985841

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Platelets and Megakaryocytes by Jonathan M. Gibbins,Martyn Mahaut-Smith Pdf

The main aim of this fourth volume is to complement the first three volumes published in 2004 and 2012 by adding advanced methodologies and perspectives. Chapters guide readers through new techniques into the study of platelets and megakaryocytes, including new imaging approaches, new methods for platelet production in vitro, and systems biology approaches. Written in the highly successful Methods in Molecular Biology series format, methods chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting or avoiding known pitfalls. Authoritative and up-to-date, Platelets and Megakaryocytes: Volume 4, Additional Protocols and Perspectives adds a wealth of new expertise for the labs of scientists working in this key biological area of study.

Author : Jonathan M. Gibbins,Martyn P. Mahaut-Smith
Publisher : Humana Press
Page : 409 pages
File Size : 46,5 Mb
Release : 2011-12-02
Category : Science
ISBN : 1617793086

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Platelets and Megakaryocytes by Jonathan M. Gibbins,Martyn P. Mahaut-Smith Pdf

New techniques to study cell signaling and function can develop at a staggering pace; however, many approaches are as valid today as on the day they were established. Thus, the main aim of Platelets and Megakaryocytes: Volume 3, Additional Protocols and Perspectives is to complement the first two volumes published in 2004 by adding recently developed state of the art techniques. Conveniently divided into three sections, this detailed volume covers techniques to study platelet function, approaches to investigate megakaryocyte function, and perspectives on important overall concepts in the field of megakaryocyte and platelet biology. Written in the highly successful Methods in Molecular BiologyTM series format, methods chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Platelets and Megakaryocytes: Volume 3, Additional Protocols and Perspectives adds a wealth of new expertise for the labs of scientists working in this key biological area of study.

Author : Gregory A. Denomme,Maria Rios,Marion E. Reid
Publisher : Elsevier
Page : 197 pages
File Size : 48,5 Mb
Release : 2000-04-27
Category : Medical
ISBN : 9780080536903

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Molecular Protocols in Transfusion Medicine by Gregory A. Denomme,Maria Rios,Marion E. Reid Pdf

This essential methods manual for immunohematologists (or hematologists and immunohematologists) provides information on genes that encode antigens on red blood cells, platelets and neutrophils. The book begins by covering general concepts in molecular biology and specific protocols such as DNA preparation, PCR-RFLP and allele-specific PCR. Information on the erythrocyte, platelet and neutrophil antigen systems and the molecular basis of polymorphisms are presented clearly in a gene facts sheet format. Database accession numbers and useful adjuncts such as Request forms, worksheets for PCR/enzyme digests also serve to benefit the user. The information is clearly presented and easily accessible and is complemented by the excellent diagrams and tabular material. This book is invaluable for both new and experienced researchers in the field and other related disciplines. Essential for hematologists and those involoved in tissue typing and the study of human genetic polymorphisms Presents clearly and concisely the information on a particular variant and the technique used to detect it Organized by antigen and provides sequences of polymorphisms and primers Details the general concepts and critical information on genes, their products, and sources of relevant nucleic acids Includes protocols that allow investigators to set up assays with minimal effort (protocols include primers, reagents, reaction conditions, sizes of amplified products, restriction fragment digests, and the relevant safety information) Provides information that helps interpret results in clinical settings Contains additional sources of information (e.g., key references, web site addresses, glossary, Database accession numbers, request forms, and worksheets for PCR/enzyme digests)

Author : Praveen Khilnani,Santosh T Soans,Vinayak Patki
Publisher : Jaypee Brothers Medical Publishers
Page : 792 pages
File Size : 42,6 Mb
Release : 2019-05-31
Category : Medical
ISBN : 9789352709694

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IAP Textbook of Pediatric ICU Protocols by Praveen Khilnani,Santosh T Soans,Vinayak Patki Pdf

Author : John T. Queenan,Catherine Y. Spong,Charles J. Lockwood
Publisher : John Wiley & Sons
Page : 507 pages
File Size : 46,7 Mb
Release : 2015-03-23
Category : Medical
ISBN : 9781119001232

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Protocols for High-Risk Pregnancies by John T. Queenan,Catherine Y. Spong,Charles J. Lockwood Pdf

High-risk pregnancies present life-threatening challenges to two of your patients: the mother and her fetus. The direct, exemplary guidance in Protocols for High-Risk Pregnancy enables you to better understand your patients' conditions devise optimum management strategies maximize the outcome and minimize the complications for both the mother and her fetus To enhance clinical relevance, each protocol is written as if the patient were present. Evidence to support an intervention is given where it exists. The authors' experience provides additional wise counsel. Key references provide the springboard for a deeper understanding of a topic. In this more compact and fully updated sixth edition, new protocols include Amniotic fluid disorders Depression Fetal growth restriction HIV Indicated late preterm and early term birth Malaria Noninvasive prenatal diagnosis Designed for clinical practice by the leaders of two generations of maternal-fetal medicine, no obstetrician or obstetric health care provider can afford to miss Protocols for High-Risk Pregnancy.

Author : Rhishikesh Thakre,Srinivas Murki
Publisher : Jaypee Brothers Medical Publishers
Page : 519 pages
File Size : 43,6 Mb
Release : 2019-11-15
Category : Medical
ISBN : 9789389188486

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Protocols in Neonatology by Rhishikesh Thakre,Srinivas Murki Pdf

Author : Jonathan M. Gibbins,Martyn P. Mahaut-Smith
Publisher : Springer Science & Business Media
Page : 0 pages
File Size : 41,8 Mb
Release : 2004-08-13
Category : Science
ISBN : 1588290115

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Platelets and Megakaryocytes by Jonathan M. Gibbins,Martyn P. Mahaut-Smith Pdf

"New techniques to study cell signaling and function can develop at a staggering pace; however, many approaches are as valid today as on the day they were established. Thus, the main aim of Platelets and Megakaryocytes: Volume 3, Additional Protocols and Perspectives is to complement the first two volumes published in 2004 by adding recently developed state of the art techniques. Conveniently divided into three sections, this detailed volume covers techniques to study platelet function, approaches to investigate megakaryocyte function, and perspectives on important overall concepts in the field of megakaryocyte and platelet biology. Written in the highly successful Methods in Molecular Biology series format, methods chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls. "--Publisher.

Author : Emmanuel J. Favaloro,Robert C. Gosselin
Publisher : Springer Nature
Page : 803 pages
File Size : 50,8 Mb
Release : 2023-05-19
Category : Medical
ISBN : 9781071631751

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Hemostasis and Thrombosis by Emmanuel J. Favaloro,Robert C. Gosselin Pdf

This second edition volume expands on the previous edition with updates about the latest state-of-the-art techniques used in leading hemostasis and thrombosis laboratories for diagnosis and exclusion of hemorrhagic and thrombotic diseases. The chapters in this book are organized into seven parts. Part One provides a general overview on hemostasis and thrombosis, preanalytical issues in testing, and routine hemostasis assays. Part Two covers laboratory testing for thrombophilia, including reviews for activated protein C resistance, protein C, lupus anticoagulant testing, and antiphospholipid antibodies. Part Three addresses monitoring continuous anticoagulant infusions and measuring the effects of oral anti-thrombotic therapy. Part Four talks about heparin induced thrombocytopenia and vaccine induced immune thrombotic thrombocytopenia. Part Five and Six cover ADAMTS13 activity testing and new information on bleeding disorders such as chromogenic factor VIII assays, measurement of emicizumab, and treatment of hemophilia A and B. Finally, Part Seven discusses global assays, research applications, and postanalytical considerations. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Hemostasis and Thrombosis: Methods and Protocols, Second Edition is a valuable resource for scientists and researchers struggling to identify the appropriate methods for hemostasis and thrombosis testing, or who seek additional expert guidance on such testing.

Author : Isabella Savini,Valeria Gasperi ,Maria Valeria Catani
Publisher : MDPI
Page : 240 pages
File Size : 44,7 Mb
Release : 2020-12-28
Category : Medical
ISBN : 9783039366903

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Molecular Research on Platelet Activity in Health and Disease by Isabella Savini,Valeria Gasperi ,Maria Valeria Catani Pdf

The present book, which includes eleven articles five reviews and six original studies published in the Special Issue “Molecular Research On Platelet Activity in Health and Disease”, gives an international picture of the up-to-date understanding of (i) platelet signaling under physiological and pathological conditions; (ii) novel technologies for monitoring platelet functions; and (iii) clinical applications of platelet-based-therapy for management of pathological conditions, not directly related to hemostasis and thrombosis. The book reveals novel aspects of platelet biology, and will be helpful for offering new insights and a research impetus for those who are interested in developing new therapeutic tools for the management of pathological conditions depending on platelet dysfunctions.

Author : Anonim
Publisher : Unknown
Page : 91 pages
File Size : 40,5 Mb
Release : 2016
Category : Electronic
ISBN : 8499052126

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Clinical Indications and Treatment Protocols with Platelet-rich Plasma in Dermatology by Anonim Pdf

Author : Nicolaus Kröger,Mohamad Mohty,Carlo Dufour
Publisher : Unknown
Page : 688 pages
File Size : 40,6 Mb
Release : 2020-10-08
Category : Medical
ISBN : 1013273672

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The EBMT Handbook by Nicolaus Kröger,Mohamad Mohty,Carlo Dufour Pdf

This Open Access edition of the European Society for Blood and Marrow Transplantation (EBMT) handbook addresses the latest developments and innovations in hematopoietic stem cell transplantation and cellular therapy. Consisting of 93 chapters, it has been written by 175 leading experts in the field. Discussing all types of stem cell and bone marrow transplantation, including haplo-identical stem cell and cord blood transplantation, it also covers the indications for transplantation, the management of early and late complications as well as the new and rapidly evolving field of cellular therapies. This book provides an unparalleled description of current practices to enhance readers' knowledge and practice skills. This work was published by Saint Philip Street Press pursuant to a Creative Commons license permitting commercial use. All rights not granted by the work's license are retained by the author or authors.

Author : Steve P. Watson,Kalwant S. Authi
Publisher : IRL Press
Page : 370 pages
File Size : 46,8 Mb
Release : 1996
Category : Medical
ISBN : 0199635374

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Platelets by Steve P. Watson,Kalwant S. Authi Pdf

Platelets are small blood cells that play a vital role in blood clotting. They are essential for wound healing, but their unwanted action can lead to ailments such as stroke and heart attack. In seventeen chapters from leading experts in platelet research this book describes state-of-the-art techniques for the experienced researcher, both in academia and industry. Methodology is also included that will enable the newcomer to perform routine experiments. Techniques covered range from basic procedures such as platelet preparation to the latest techniques such as monitoring calcium levels in single cells, protein tyrosine phosphorylation, and PCR.

Author : Brian R. Peterson
Publisher : Nova Publishers
Page : 200 pages
File Size : 46,6 Mb
Release : 2006
Category : Blood
ISBN : 1594549621

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New Developments in Blood Transfusion Research by Brian R. Peterson Pdf

Although blood transfusion saves lives and reduces morbidities in many clinical diseases and conditions, it is associated with certain risks. A transfusion-related adverse event, also called transfusion reaction, is any unfavourable event occurring in a patient during or after blood transfusion. About 0.5 per cent to 3 per cent of all transfusions result in some adverse events, but the majority of them are minor reactions with no significant consequences. In general, transfusion-related adverse events are categorised as infectious and non-infectious. However, there are other classifications in the literature based on time of occurrence (i.e. acute versus delayed) or physiological mechanism (i.e. immune mediated versus non-immune mediated). A significant proportion of adverse events may occur as a result of errors in preparation, ordering or administration of blood and blood products. This book contains the latest research in this essential field which has been revolutionised in recent decades.