Practical Fluorescence Spectroscopy

Author : Zygmunt (Karol) Gryczynski,Ignacy Gryczynski
Publisher : CRC Press
Page : 772 pages
File Size : 46,9 Mb
Release : 2019-12-18
Category : Medical
ISBN : 9781439821701

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Practical Fluorescence Spectroscopy by Zygmunt (Karol) Gryczynski,Ignacy Gryczynski Pdf

Presenting a detailed, hands-on approach to fluorescence spectroscopy, this book describes experiments that cover basic spectroscopy and advanced aspects of fluorescence spectroscopy. It emphasizes practical guidance, providing background on fundamental concepts as well as guidance on how to handle artifacts, avoid common errors, and interpret data. Nearly 150 experiments from biophysics, biochemistry, and the biomedical sciences demonstrate how methods are applied in practical applications. The result is a hands-on guide to the most important aspects of fluorescence spectroscopy, from steady-state fluorescence to advanced time-resolved fluorescence. Provides a complete overview of nearly 150 experiments using fluorescence spectroscopy, from basic to advanced applications Presents laboratory methods using a variety of instrumental setups with detailed discussion of data analysis and interpretations Covers steady-state phenomena, time-resolved phenomena, and advanced methods Spans biophysical, biochemical, and biomedical applications Describes related concepts, theory, and mathematical background as well as commercially available instruments used for measurements

Author : George G. Guilbault
Publisher : CRC Press
Page : 918 pages
File Size : 51,8 Mb
Release : 2020-08-26
Category : Science
ISBN : 9781000147650

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Practical Fluorescence, Second Edition by George G. Guilbault Pdf

This is a new edition (first, 1973) of an introduction to the principles and applications of all phases of luminescence spectroscopy. Contains (all rewritten) chapters on general aspects of luminescence, instrumentation, effects of molecular structure and environment, inorganic analysis and phosphorescence. The second edition also introduces new topics such as process, applications, bioprocess monitoring and biotechnology methods, soild surface luminescence and pesticide analysis, providing expanded coverage on chemiluminescence and environmental analysis and updates information on equipment, supplies newer references and more.

Author : George G. Guilbault
Publisher : Unknown
Page : 688 pages
File Size : 45,9 Mb
Release : 1973
Category : Science
ISBN : UOM:39015047288421

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Practical Fluorescence; Theory, Methods, and Techniques by George G. Guilbault Pdf

Author : Yves Engelborghs,Antonie J.W.G. Visser
Publisher : Humana
Page : 0 pages
File Size : 55,8 Mb
Release : 2016-08-23
Category : Medical
ISBN : 1493963155

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Fluorescence Spectroscopy and Microscopy by Yves Engelborghs,Antonie J.W.G. Visser Pdf

Reflecting the expanding field’s need for reliable protocols, Fluorescence Spectroscopy and Microscopy: Methods and Protocols offers techniques from a worldwide team of experts on this versatile and vital subject. The topics covered fall into four broad categories: steady-state fluorescence spectroscopy, time-resolved fluorescence spectroscopy, fluorescent probe development, and the various sub-categories of fluorescence microscopy, such as fluorescence recovery after photobleaching (FRAP), live cell FRET imaging (FRETim), fluorescence lifetime imaging (FLIM), fluorescence fluctuation spectroscopy (FFS), and single-molecule fluorescence spectroscopy (smFS). Written as a part of the popular Methods in Molecular Biology series, chapters include the kind of unambiguous detail and key implementation advice that proves essential for successful results. Comprehensive and practical, Fluorescence Spectroscopy and Microscopy: Methods and Protocols aims to guide both ‘novice’ and established scientists toward furthering their research with these invaluable techniques.

Author : Burkhard Beckhoff,Birgit Kanngießer,Norbert Langhoff,Reiner Wedell,Helmut Wolff
Publisher : Springer Science & Business Media
Page : 897 pages
File Size : 41,7 Mb
Release : 2007-05-18
Category : Science
ISBN : 9783540367222

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Handbook of Practical X-Ray Fluorescence Analysis by Burkhard Beckhoff,Birgit Kanngießer,Norbert Langhoff,Reiner Wedell,Helmut Wolff Pdf

X-Ray fluorescence analysis is an established technique for non-destructive elemental materials analysis. This book gives a user-oriented practical guidance to the application of this method. The book gives a survey of the theoretical fundamentals, analytical instrumentation, software for data processing, various excitation regimes including gracing incidents and microfocus measurements, quantitative analysis, applications in routine and micro analysis, mineralogy, biology, medicine, criminal investigations, archeology, metallurgy, abrasion, microelectronics, environmental air and water analysis. This book is the bible of X-Ray fluorescence analysis. It gives the basic knowledge on this technique, information on analytical equipment and guides the reader to the various applications. It appeals to researchers, analytically active engineers and advanced students.

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
Page : 961 pages
File Size : 45,5 Mb
Release : 2007-12-05
Category : Science
ISBN : 9780387463124

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Principles of Fluorescence Spectroscopy by Joseph R. Lakowicz Pdf

The third edition of this established classic text reference builds upon the strengths of its very popular predecessors. Organized as a broadly useful textbook Principles of Fluorescence Spectroscopy, 3rd edition maintains its emphasis on basics, while updating the examples to include recent results from the scientific literature. The third edition includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. Includes a link to Springer Extras to download files reproducing all book artwork, for easy use in lecture slides. This is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.

Author : Jihad Rene Albani
Publisher : John Wiley & Sons
Page : 264 pages
File Size : 46,5 Mb
Release : 2008-04-15
Category : Science
ISBN : 9780470691335

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Principles and Applications of Fluorescence Spectroscopy by Jihad Rene Albani Pdf

Fluorescence spectroscopy is an important investigational tool in many areas of analytical science, due to its extremely high sensitivity and selectivity. With many uses across a broad range of chemical, biochemical and medical research, it has become an essential investigational technique allowing detailed, real-time observation of the structure and dynamics of intact biological systems with extremely high resolution. It is particularly heavily used in the pharmaceutical industry where it has almost completely replaced radiochemical labelling. Principles and Applications of Fluorescence Spectroscopy gives the student and new user the essential information to help them to understand and use the technique confidently in their research. By integrating the treatment of absorption and fluorescence, the student is shown how fluorescence phenomena arise and how these can be used to probe a range of analytical problems. A key element of the book is the inclusion of practical laboratory experiments that illustrate the fundamental points and applications of the technique.

Author : G. Guilbault
Publisher : Unknown
Page : 128 pages
File Size : 52,9 Mb
Release : 1973
Category : Electronic
ISBN : OCLC:989550769

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Practical Fluorescence : Theory Methods and Techniques by G. Guilbault Pdf

Author : George G. Guilbault
Publisher : CRC Press
Page : 825 pages
File Size : 41,9 Mb
Release : 2020-08-26
Category : Science
ISBN : 9781000104448

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Practical Fluorescence, Second Edition by George G. Guilbault Pdf

This is a new edition (first, 1973) of an introduction to the principles and applications of all phases of luminescence spectroscopy. Contains (all rewritten) chapters on general aspects of luminescence, instrumentation, effects of molecular structure and environment, inorganic analysis and phosphorescence. The second edition also introduces new topics such as process, applications, bioprocess monitoring and biotechnology methods, soild surface luminescence and pesticide analysis, providing expanded coverage on chemiluminescence and environmental analysis and updates information on equipment, supplies newer references and more.

Author : Michael Haschke,Jörg Flock,Michael Haller
Publisher : John Wiley & Sons
Page : 496 pages
File Size : 45,8 Mb
Release : 2021-04-05
Category : Science
ISBN : 9783527344635

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X-Ray Fluorescence Spectroscopy for Laboratory Applications by Michael Haschke,Jörg Flock,Michael Haller Pdf

Provides comprehensive coverage on using X-ray fluorescence for laboratory applications This book focuses on the practical aspects of X-ray fluorescence (XRF) spectroscopy and discusses the requirements for a successful sample analysis, such as sample preparation, measurement techniques and calibration, as well as the quality of the analysis results. X-Ray Fluorescence Spectroscopy for Laboratory Applications begins with a short overview of the physical fundamentals of the generation of X-rays and their interaction with the sample material, followed by a presentation of the different methods of sample preparation in dependence on the quality of the source material and the objective of the measurement. After a short description of the different available equipment types and their respective performance, the book provides in-depth information on the choice of the optimal measurement conditions and the processing of the measurement results. It covers instrument types for XRF; acquisition and evaluation of X-Ray spectra; analytical errors; analysis of homogeneous materials, powders, and liquids; special applications of XRF; process control and automation. An important resource for the analytical chemist, providing concrete guidelines and support for everyday analyses Focuses on daily laboratory work with commercially available devices Offers a unique compilation of knowledge and best practices from equipment manufacturers and users Covers the entire work process: sample preparation, the actual measurement, data processing, assessment of uncertainty, and accuracy of the obtained results X-Ray Fluorescence Spectroscopy for Laboratory Applications appeals to analytical chemists, analytical laboratories, materials scientists, environmental chemists, chemical engineers, biotechnologists, and pharma engineers.

Author : George G. Guilbault
Publisher : Unknown
Page : 664 pages
File Size : 46,8 Mb
Release : 1973
Category : Electronic
ISBN : OCLC:498487618

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PRACTICAL FLUORESCENCE by George G. Guilbault Pdf

Author : Ashutosh Sharma,Stephen G. Schulman
Publisher : Wiley-Interscience
Page : 200 pages
File Size : 51,8 Mb
Release : 1999-05-21
Category : Science
ISBN : UOM:39015050036279

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Introduction to Fluorescence Spectroscopy by Ashutosh Sharma,Stephen G. Schulman Pdf

An accessible guide to all aspects of molecular fluorescence spectroscopy This book introduces the uninitiated reader to the growing body of analytical methods based on molecular fluorescence. Geared to practitioners with no particular training or exposure to the field, it highlights fluorescence spectroscopy's tremendous appeal in present-day pharmaceutical, biomedical, and environmental analysis. Written by two highly respected experts in the field, Introduction to Fluorescence Spectroscopy covers all aspects of the technology-physical fundamentals, instrumentation, methods, and applications. The information is offered at 0a very practical level and addresses a broad range of chemical, physical, biological, and geological problems. The authors incorporate recent advances in commercially available instrumentation as well as fluorescent derivatizing agents, provide many examples of state-of-the-art applications, and discuss future trends. Concise, accessible, up-to-date, Introduction to Fluorescence Spectroscopy is an indispensable reference and an invaluable primer for those involved in the field of analytical science and other professionals interested in this fast-evolving analytical technique.

Author : Chris Gell,David Brockwell,Alastair Smith
Publisher : OUP Oxford
Page : 278 pages
File Size : 46,6 Mb
Release : 2006-08-17
Category : Science
ISBN : 9780191523854

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Handbook of Single Molecule Fluorescence Spectroscopy by Chris Gell,David Brockwell,Alastair Smith Pdf

Analytical measurements at the single molecule level under ambient conditions have become almost routine in the past few years. The application of this technology to fundamental studies of heterogeneity in biomolecular structure and dynamics, chemical and biological reaction kinetics, and photophysics provides a rich playground for molecular scientists. The potential use of single molecule detection for nanotechnology and quantum information processing is a new and almost unexplored area. This handbook is intended for those interested in a practical introduction to single molecule investigations using fluorescence techniques and places special emphasis on the practicalities of achieving single molecule resolution, analysing the resulting data and exploration of the applications in biophysics. It is ideal for graduate research students and others embarking on work in this exciting field.

Author : Chris Gell,David Brockwell,Alastair Smith
Publisher : Oxford University Press on Demand
Page : 279 pages
File Size : 44,7 Mb
Release : 2006-08-17
Category : Science
ISBN : 9780198529422

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Handbook of Single Molecule Fluorescence Spectroscopy by Chris Gell,David Brockwell,Alastair Smith Pdf

This is a practical introduction to single molecule fluorescence experiments, the analysis of the data, and applications of the techniques to the study of biological structure and function.

Author : Mike Gore
Publisher : OUP Oxford
Page : 387 pages
File Size : 52,5 Mb
Release : 2000-05-04
Category : Science
ISBN : 9780191566059

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Spectrophotometry and Spectrofluorimetry by Mike Gore Pdf

Spectrophotometry and Spectrofluorimetry: A Practical Approach Second Edition was written with the intention to help the reader understand the background concepts and practical applications of spectrophotometry and spectrofluorimetry. Optical spectroscopy underpins the day to day operations of most laboratories in the chemical, biological and medical sciences and this edition contains substantially updated and new chapters addressing the principles of most of the more common applications such as: spectrophotometry, spectrophotometric assays, spectrofluorimetry, time resolved fluorescence and phosphorescence studies, circular dichrosim and pre-equilibrium spectroscopic techniques. In all chapters, the emphasis is placed upon the practical aspects, with protocols to guide readers through test experiments. Other chapters are included to introduce subjects that have traditionally depended upon spectroscopy such as basic enzyme kinetics, ligand binding, data handling and the more recently established interest in the study of protein and DNA stability. Finally, the concept of 'global analysis' is introduced to provide the reader with an insight into this method of utilizing the vast arrays of experimental data provided by current instrumentation.