Protein Engineering For Industrial Biotechnology

Author : Lilia Alberghina
Publisher : CRC Press
Page : 374 pages
File Size : 50,7 Mb
Release : 2003-09-02
Category : Medical
ISBN : 9780203303702

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Protein Engineering For Industrial Biotechnology by Lilia Alberghina Pdf

Protein engineering has proved to be one of the more fruitful technological approaches in biotechnology, being both very powerful and able to generate valuable intellectual property. This book aims to present examples in which the application of protein engineering has successfully solved problems arising in industrial biotechnology. There is a section on its use to enhance purification of recombinant proteins. The use of protein engineering to modify the activity or the stability of industrial enzymes from lipases to proteases, from carboxypeptidases to glucanases and glucosidases, and from pectin modifying enzymes to enzymes able to degrade recalcitrant compounds is extensively covered. It is shown how areas as diverse as agrofood technology, fine chemistry, detergents, bioremediation and biosensors receive significant contributions from protein and solvent engineering. The application of protein engineering to health care is also covered, from the development of new vaccines to new potential therapeutic proteins. A specific notation is given to protein engineering in the development of target molecules for drug discovery. International in scope, the many contributions are drawn from academia and industry. The text should be of interest to students and researchers in industrial biotechnology as well as to everybody interested in basic research in protein structure, molecular genetics, bio-organic chemistry, biochemistry, agrobiotechnology, pharmaceutical sciences and medicine.

Author : Huimin Zhao
Publisher : John Wiley & Sons
Page : 41 pages
File Size : 51,7 Mb
Release : 2021-08-23
Category : Science
ISBN : 9783527344703

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Protein Engineering by Huimin Zhao Pdf

A one-stop reference that reviews protein design strategies to applications in industrial and medical biotechnology Protein Engineering: Tools and Applications is a comprehensive resource that offers a systematic and comprehensive review of the most recent advances in the field, and contains detailed information on the methodologies and strategies behind these approaches. The authors—noted experts on the topic—explore the distinctive advantages and disadvantages of the presented methodologies and strategies in a targeted and focused manner that allows for the adaptation and implementation of the strategies for new applications. The book contains information on the directed evolution, rational design, and semi-rational design of proteins and offers a review of the most recent applications in industrial and medical biotechnology. This important book: Covers technologies and methodologies used in protein engineering Includes the strategies behind the approaches, designed to help with the adaptation and implementation of these strategies for new applications Offers a comprehensive and thorough treatment of protein engineering from primary strategies to applications in industrial and medical biotechnology Presents cutting edge advances in the continuously evolving field of protein engineering Written for students and professionals of bioengineering, biotechnology, biochemistry, Protein Engineering: Tools and Applications offers an essential resource to the design strategies in protein engineering and reviews recent applications.

Author : Caroline Koehrer,Uttam L. RajBhandary
Publisher : Springer Science & Business Media
Page : 351 pages
File Size : 55,5 Mb
Release : 2009-01-07
Category : Science
ISBN : 9783540709411

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Protein Engineering by Caroline Koehrer,Uttam L. RajBhandary Pdf

Site-specific mutagenesis of DNA, developed some thirty years ago, has proven to be one of the most important advances in biology. By allowing the site-specific replacement of any amino acid in a protein with one of the other nineteen amino acids, it ushered in the new era of "Protein Engineering". The field of protein engineering has, however, evolved rapidly since then and the last fifteen years have witnessed remarkable advances through the use of new chemical, biochemical and molecular biological tools towards the synthesis and manipulation of proteins. The chapters included in this book reflect the rapid evolution of protein engineering and its many applications in basic research, biotechnology, material sciences and therapy. This book will provide the reader with an introduction to state-of the-art concepts and methods and will be of use to anyone interested in the study of proteins, in academia as well as in industry.

Author : Raghupathy Sarma
Publisher : Elsevier
Page : 441 pages
File Size : 43,7 Mb
Release : 2012-12-02
Category : Technology & Engineering
ISBN : 9780323150309

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Protein Engineering by Raghupathy Sarma Pdf

Protein Engineering: Applications in Science, Medicine, and Industry deals with the scientific, medical, and industrial applications of protein engineering. Topics range from protein structure and design to mutant analysis and complex systems. Applications such as production of novel antibiotics, genetic transformation of plants, and genetic engineering of bioinsecticides are described. This book is comprised of 25 chapters and begins with an overview of trends and developments in protein chemistry and their relevance to protein engineering, followed by a discussion on protein sequence data banks. Subsequent chapters explore the design and construction of biologically active peptides, including hormones; structural and functional analysis of thermophile proteins; the conformation of diphtheria toxin; and applications of surface-simulation synthesis in protein molecular recognition. The use of oligonucleotide-directed site-specific mutagenesis in functional analysis of the signal peptide for protein secretion is also considered. The results of studies on the mechanism of membrane fusion are presented. This monograph will serve as a useful guide for those who are already working on protein engineering and those who are about to start research in this field.

Author : Anonim
Publisher : Unknown
Page : 128 pages
File Size : 48,6 Mb
Release : 1988
Category : Electronic
ISBN : OCLC:437052471

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Enzymes for Biotechnology by Anonim Pdf

Author : Kristian Müller,Katja Arndt
Publisher : Springer Science & Business Media
Page : 318 pages
File Size : 48,6 Mb
Release : 2007-10-26
Category : Science
ISBN : 9781597451871

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Protein Engineering Protocols by Kristian Müller,Katja Arndt Pdf

Protein engineering is a fascinating mixture of molecular biology, protein structure analysis, computation, and biochemistry, with the goal of developing useful or valuable proteins. Protein Engineering Protocols will consider the two general, but not mutually exclusive, strategies for protein engineering. The first is known as rational design, in which the scientist uses detailed knowledge of the structure and function of the protein to make desired changes. The s- ond strategy is known as directed evolution. In this case, random mutagenesis is applied to a protein, and selection or screening is used to pick out variants that have the desired qualities. By several rounds of mutation and selection, this method mimics natural evolution. An additional technique known as DNA shuffling mixes and matches pieces of successful variants to produce better results. This process mimics recombination that occurs naturally during sexual reproduction. The first section of Protein Engineering Protocols describes rational p- tein design strategies, including computational methods, the use of non-natural amino acids to expand the biological alphabet, as well as impressive examples for the generation of proteins with novel characteristics. Although procedures for the introduction of mutations have become routine, predicting and und- standing the effects of these mutations can be very challenging and requires profound knowledge of the system as well as protein structures in general.

Author : P. R. Shewry,S. Gutteridge
Publisher : Cambridge University Press
Page : 366 pages
File Size : 42,5 Mb
Release : 1992-08-20
Category : Medical
ISBN : 0521417619

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Plant Protein Engineering by P. R. Shewry,S. Gutteridge Pdf

This first volume of the plant and microbial biotechnology series introduces the concepts and potential of plant protein engineering and gives an account of current research in the field. An essential purchase for academic and industrial research institutions and professional biotechnologists.

Author : Stefan Lutz,Uwe Theo Bornscheuer
Publisher : John Wiley & Sons
Page : 723 pages
File Size : 42,9 Mb
Release : 2012-09-14
Category : Science
ISBN : 9783527666980

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Protein Engineering Handbook, Volume 3 by Stefan Lutz,Uwe Theo Bornscheuer Pdf

This introduction collects 17 innovative approaches to engineer novel and improved proteins for diverse applications in biotechnology, chemistry, bioanalytics and medicine. As such, key developments covered in this reference and handbook include de novo enzyme design, cofactor design and metalloenzymes, extremophile proteins, and chemically resistant proteins for industrial processes. The editors integrate academic innovations and industrial applications so as to arrive at a balanced view of this multi-faceted topic. Throughout, the content is chosen to complement and extend the previously published two-volume handbook by the same editors, resulting in a superb overview of this burgeoning field.

Author : Anonim
Publisher : Unknown
Page : 128 pages
File Size : 53,9 Mb
Release : 2017
Category : Electronic
ISBN : 1785693468

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PROTEIN ENGINEERING by Anonim Pdf

Author : Peter C. E. Moody,Tony Wilkinson
Publisher : Oxford University Press, USA
Page : 104 pages
File Size : 47,5 Mb
Release : 1990
Category : Science
ISBN : UOM:39015021881308

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Protein Engineering by Peter C. E. Moody,Tony Wilkinson Pdf

The aim of protein engineering is to improve or alter the properties of proteins in a rational, pre-determined way. This requires an understanding of the scope, structure, and function of proteins. The increasing importance of the subject is reflected in the widening range of courses coveringthe topic. This book provides a clear, up-to-date review of the subject and explains the principles and applications. Topics covered include analysis of mutant proteins, understanding of structure-activity relationships, and the application of protein engineering to industrial and medical problems.

Author : Stefan Lutz,Uwe Theo Bornscheuer
Publisher : Wiley-VCH
Page : 0 pages
File Size : 43,7 Mb
Release : 2012-11-28
Category : Science
ISBN : 3527331239

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Protein Engineering Handbook, Volume 3 by Stefan Lutz,Uwe Theo Bornscheuer Pdf

This introduction collects 17 innovative approaches to engineer novel and improved proteins for diverse applications in biotechnology, chemistry, bioanalytics and medicine. As such, key developments covered in this reference and handbook include de novo enzyme design, cofactor design and metalloenzymes, extremophile proteins, and chemically resistant proteins for industrial processes. The editors integrate academic innovations and industrial applications so as to arrive at a balanced view of this multi-faceted topic. Throughout, the content is chosen to complement and extend the previously published two-volume handbook by the same editors, resulting in a superb overview of this burgeoning field.

Author : Anonim
Publisher : Unknown
Page : 128 pages
File Size : 54,5 Mb
Release : 1990
Category : Enzymes
ISBN : OCLC:19267081

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Enzymes for Biotechnology by Anonim Pdf

Author : Carmichael J.A. Wallace
Publisher : CRC Press
Page : 300 pages
File Size : 53,9 Mb
Release : 1999-09-29
Category : Medical
ISBN : 0849347270

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Protein Engineering by Semisynthesis by Carmichael J.A. Wallace Pdf

As genomics gives way to proteomics as the focus of scientific imagination in the biological sciences, more emphasis will be placed on the technology and interpretation of protein engineering experiments. Protein engineers will become increasingly sophisticated in the questions that they pose and demanding of the tools available to change protein structure. The optimal way to introduce non-coded amino acids for mechanistic studies, or site-specific reporter atoms for spectroscopic structural biology, is by protein semisynthesis. In Protein Engineering by Semisynthesis, the leading practitioners of the method cover their individual protein of expertise forming a comprehensive illustration of the various methods developed. By covering the most recent philosophical and methodological approaches and developments of semisynthesis and peptide synthesis to date, this book provides further understanding of the principles of protein structure-function relationships gained from semisynthetic analog in addition to providing a comprehensive and comprehensible laboratory guide. This book focuses on recent developments which synergistically combine chemical and molecular biological techniques that have made semisynthetic manipulations much easier to undertake. Features

Author : Manfred T. Reetz,Zhoutong Sun,Ge Qu
Publisher : John Wiley & Sons
Page : 405 pages
File Size : 52,7 Mb
Release : 2023-01-10
Category : Science
ISBN : 9783527350339

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Enzyme Engineering by Manfred T. Reetz,Zhoutong Sun,Ge Qu Pdf

Enzyme Engineering An authoritative and up-to-date discussion of enzyme engineering and its applications In Enzyme Engineering: Selective Catalysts for Applications in Biotechnology, Organic Chemistry, and Life Science, a team of distinguished researchers deliver a robust treatment of enzyme engineering and its applications in various fields such as biotechnology, life science, and synthesis. The book begins with an introduction to different protein engineering techniques, covers topics like gene mutagenesis methods for directed evolution and rational enzyme design. It includes industrial case studies of enzyme engineering with a focus on selectivity and activity. The authors also discuss new and innovative areas in the field, involving machine learning and artificial intelligence. It offers several insightful perspectives on the future of this work. Readers will also find: A thorough introduction to directed evolution and rational design as protein engineering techniques Comprehensive explorations of screening and selection techniques, gene mutagenesis methods in directed evolution, and guidelines for applying gene mutagenesis in organic chemistry, pharmaceutical applications, and biotechnology Practical discussions of protein engineering of enzyme robustness relevant to organic and pharmaceutical chemistry Treatments of artificial enzymes as promiscuous catalysts Various lessons learned from semi-rational and rational directed evolution A transdisciplinary treatise, Enzyme Engineering: Selective Catalysts for Applications in Biotechnology, Organic Chemistry, and Life Science is perfect for protein engineers, theoreticians, organic, and pharmaceutical chemists as well as transition metal researchers in catalysis and biotechnologists.

Author : Tuck Seng Wong,Kang Lan Tee
Publisher : Springer Nature
Page : 213 pages
File Size : 49,6 Mb
Release : 2020-10-29
Category : Science
ISBN : 9783030568986

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A Practical Guide to Protein Engineering by Tuck Seng Wong,Kang Lan Tee Pdf

This textbook introduces readers in an accessible and engaging way to the nuts and bolts of protein expression and engineering. Various case studies illustrate each step from the early sequence searches in online databases over plasmid design and molecular cloning techniques to protein purification and characterization. Furthermore, readers are provided with practical tips to successfully pursue a career as a protein engineer. With protein engineering being a fundamental technique in almost all molecular biology labs, the book targets advanced undergraduates and graduate students working in molecular biology, biotechnology and related scientific fields.