Protein Stability And Folding

Author : Bret A. Shirley
Publisher : Unknown
Page : 377 pages
File Size : 53,9 Mb
Release : 1995
Category : Science
ISBN : 1592595278

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Protein Stability and Folding by Bret A. Shirley Pdf

In Protein Stability and Folding: Theory and Practice, world-class scientists present in a single volume a comprehensive selection of hands-on recipes for all of the major techniques needed to understand the conformational stability of proteins, as well as their three-dimensional folding. The distinguished contributors provide clear, step-by-step instructions along with many troubleshooting tips, alternative procedures, and informative explanations about why certain steps are necessary. Even highly skilled researchers will find many time-saving methods. Among the techniques discussed are fluorescent, ultraviolet, and infrared spectroscopy; HPLC peptide mapping; differential scanning calorimetry; and hydrogen exchange. Shirley's Protein Stability and Folding: Theory and Practice will ensure a significant difference in the outcome of your experiments, producing the result desired even for beginners.

Author : Bret A. Shirley
Publisher : Taylor & Francis US
Page : 392 pages
File Size : 45,8 Mb
Release : 1995-03-24
Category : Medical
ISBN : 0896033015

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Protein Stability and Folding by Bret A. Shirley Pdf

In Protein Stability and Folding: Theory and Practice, world-class scientists present in a single volume a comprehensive selection of hands-on recipes for all of the major techniques needed to understand the conformational stability of proteins, as well as their three-dimensional folding. The distinguished contributors provide clear, step-by-step instructions along with many troubleshooting tips, alternative procedures, and informative explanations about why certain steps are necessary. Even highly skilled researchers will find many time-saving methods. Among the techniques discussed are fluorescent, ultraviolet, and infrared spectroscopy; HPLC peptide mapping; differential scanning calorimetry; and hydrogen exchange. Shirley's Protein Stability and Folding: Theory and Practice will ensure a significant difference in the outcome of your experiments, producing the result desired even for beginners.

Author : Kenneth P. Murphy
Publisher : Springer Science & Business Media
Page : 258 pages
File Size : 54,7 Mb
Release : 2008-02-04
Category : Science
ISBN : 9781592591930

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Protein Structure, Stability, and Folding by Kenneth P. Murphy Pdf

In Protein Structure, Stability, and Folding, Kenneth P. Murphy and a panel of internationally recognized investigators describe some of the newest experimental and theoretical methods for investigating these critical events and processes. Among the techniques discussed are the many methods for calculating many of protein stability and dynamics from knowledge of the structure, and for performing molecular dynamics simulations of protein unfolding. New experimental approaches presented include the use of co-solvents, novel applications of hydrogen exchange techniques, temperature-jump methods for looking at folding events, and new strategies for mutagenesis experiments. Unique in its powerful combination of theory and practice, Protein Structure, Stability, and Folding offers protein and biophysical chemists the means to gain a more comprehensive understanding of some of this complex area by detailing many of the major techniques in use today.

Author : Wolfgang Pfeil
Publisher : Springer Science & Business Media
Page : 662 pages
File Size : 53,9 Mb
Release : 2012-12-06
Category : Science
ISBN : 9783642587603

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Protein Stability and Folding by Wolfgang Pfeil Pdf

Protein folding remains one of the most exclusive problems of modern biochemistry. Structure analysis has given access to the wealth of the molecular architecture of pro teins. As architecture needs static calculations, protein structure is always related to thermodynamic factors that govern folding and stability of a particular folded protein over the non-organized polypeptide chain. During the past decades a huge amount of thermodynamic data related to protein folding and stability has been accumulated. The data are certainly of importance in dechiffring the protein folding problem. At the same time, the data can guide the con struction of modified and newly synthesized proteins with properties optimized for particular application. The intention of this book is a generation of a data collection which makes the vast amount of present data accessible for multidisciplinary research where chemistry, phy sics, biology, and medicine are involved and also pharmaceutical and food research and technology. It took several years to compile all the data and the author wishes to thank everyone who provided data, ideas or even unpublished results. The author is, in particular, indebted to Prof. Wadso (Lund, Sweden) and IUPAC's Steering Committee on Bio physical Chemistry. Furthermore, support by the Deutsche Forschungsgemeinschafi (INK 16 AI-I) is acknowledged.

Author : Wolfgang Pfeil
Publisher : Springer Science & Business Media
Page : 524 pages
File Size : 42,5 Mb
Release : 2013-06-29
Category : Science
ISBN : 9783662128381

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Protein Stability and Folding by Wolfgang Pfeil Pdf

In 1998, we published the data compilation PROTEIN STABILITY AND FOLDING which covered the data from the early beginnings of thermodynamic studies of protein folding until 1996. Since then, the amount of available thermodynamic data has increased nearly twice. The data constitute very important additions to the information on the protein folding problem, the construction of mutant protein, and the practical application of proteins in various fields. The Supplement covers the period 1997-1999 and is designed to make the vast amount of present data accessible to multidisciplinary research where chemistry, physics, biology, and medicine are involved and also biotechnology, pharmaceutical and food research. At the same time the data could be helpful to identify problems unsolved so far, and to avoid unnecessary duplication of scientific work. The structure of the Supplement is the same as in the previous data compilation. However, some additional data characterizing protein-denaturant interaction and protein unfolding by trifluoroethanol have been added. In that context, some previous data have been reconsidered. The author wishes to thank everyone who provided data, ideas, or even unpublished results. Furthermore, support by the Deutsche Forschungsgemeinschaft (INK 16 BI-I) is gratefully acknowledged. Finally, I would like to thank the staff of Springer Verlag for their efforts and for excellent assistance during the production of the data collections.

Author : Prakash Saudagar,Timir Tripathi
Publisher : Springer Nature
Page : 287 pages
File Size : 53,6 Mb
Release : 2023-05-27
Category : Science
ISBN : 9789819920792

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Protein Folding Dynamics and Stability by Prakash Saudagar,Timir Tripathi Pdf

This book describes recent important advancements in protein folding dynamics and stability research, as well as explaining fundamentals and examining potential methodological approaches in protein science. In vitro, in silico, and in vivo method based research of how the stability and folding of proteins help regulate the cellular dynamics and impact cell function that are crucial in explaining various physiological and pathological processes. This book offers a comprehensive coverage on various techniques and related recent developments in the experimental and computational methods of protein folding, dynamics, and stability studies. The book is also structured in such a way as to summarize the latest developments in the fiddle and key concepts to ensure that readers can understand advanced concepts as well as the fundamental big picture. And most of all, fresh insights are provided into the convergence of protein science and technology. Protein Folding Dynamics and Stability is an ideal guide to the field that will be of value for all levels of researchers and advanced graduate students with training in biochemical laboratory research.

Author : Anonim
Publisher : Unknown
Page : 521 pages
File Size : 49,7 Mb
Release : 1998
Category : Protein folding
ISBN : OCLC:873226887

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Protein Stability and Folding by Anonim Pdf

Author : Matthias J Feige
Publisher : Royal Society of Chemistry
Page : 450 pages
File Size : 44,9 Mb
Release : 2018-07-30
Category : Science
ISBN : 9781782629900

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Oxidative Folding of Proteins by Matthias J Feige Pdf

The formation of disulphide bonds is probably the most influential modification of proteins. These bonds are unique among post-translational modifications of proteins as they can covalently link cysteine residues far apart in the primary sequence of a protein. This has the potential to convey stability to otherwise marginally stable structures of proteins. However, the reactivity of cysteines comes at a price: the potential to form incorrect disulphide bonds, interfere with folding, or even cause aggregation. An elaborate set of cellular machinery exists to catalyze and guide this process: facilitating bond formation, inhibiting unwanted pairings and scrutinizing the outcomes. Only in recent years has it become clear how intimately connected this cellular machinery is with protein folding helpers, organellar redox balance and cellular homeostasis as a whole. This book comprehensively covers the basic principles of disulphide bond formation in proteins and describes the enzymes involved in the correct oxidative folding of cysteine-containing proteins. The biotechnological and pharmaceutical relevance of proteins, their variants and synthetic replicates is continuously increasing. Consequently this book is an invaluable resource for protein chemists involved in realted research and production.

Author : Cláudio M. Gomes,Patrícia F.N. Faísca
Publisher : Springer
Page : 63 pages
File Size : 54,5 Mb
Release : 2019-02-25
Category : Science
ISBN : 9783319008820

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Protein Folding by Cláudio M. Gomes,Patrícia F.N. Faísca Pdf

This snapshot volume is designed to provide a smooth entry into the field of protein folding. Presented in a concise manner, each section introduces key concepts while providing a brief overview of the relevant literature. Outlook subsections will pinpoint specific aspects related to emerging methodologies, concepts and trends.

Author : Alka Dwevedi
Publisher : Springer
Page : 55 pages
File Size : 40,9 Mb
Release : 2014-12-01
Category : Science
ISBN : 9783319125923

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Protein Folding by Alka Dwevedi Pdf

The book will discuss classes of proteins and their folding, as well as the involvement of bioinformatics in solving the protein folding problem. In vivo and in vitro folding mechanisms are examined, as well as the failures of in vitro folding, a mechanism helpful in understanding disease caused by misfolding. The role of energy landscapes is also discussed and the computational approaches to these landscapes.

Author : Regina Murphy,Amos Tsai
Publisher : Springer Science & Business Media
Page : 354 pages
File Size : 55,8 Mb
Release : 2007-10-12
Category : Science
ISBN : 9780387360638

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Misbehaving Proteins by Regina Murphy,Amos Tsai Pdf

This text provides an up-to-date collection of theoretical and experimental studies into protein folding, misfolding, aggregation, and stability. Additionally, issues faced during the development of protein products are illustrated. It contains an introductory chapter for readers new to the protein folding field. The book provides a thorough and clear discussion of computational approaches to understanding and modeling protein aggregation.

Author : Charis Ghelis
Publisher : Academic Press
Page : 580 pages
File Size : 52,9 Mb
Release : 2012-12-02
Category : Science
ISBN : 9780323140928

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Protein Folding by Charis Ghelis Pdf

Protein Folding aims to collect the most important information in the field of protein folding and probes the main principles that govern formation of the three-dimensional structure of a protein from a nascent polypeptide chain, as well as how the functional properties appear. This text is organized into three sections and consists of 15 chapters. After an introductory chapter where the main problems of protein folding are considered at the cellular level in the context of protein biosynthesis, the discussion turns to the conformation of native globular proteins. Definitions and rules of nomenclature are given, including the structural organization of globular proteins deduced from X-ray crystallographic data. Folding mechanisms are tentatively deduced from the observation of invariants in the architecture of folded proteins. The next chapters focus on the energetics of protein conformation and structure, indicating the principles of thermodynamic stability of the native structure, along with theoretical computation studies of protein folding, structure prediction, and folding simulation. The reader is also introduced to various experimental approaches; the reversibility of the unfolding-folding process; equilibrium and kinetic studies; and detection and characterization of intermediates in protein folding. This text concludes with a chapter dealing with problems specific to oligomeric proteins. This book is intended for research scientists, specialists, biochemists, and students of biochemistry and biology.

Author : David S. Eisenberg,Christian B. Anfinsen,Frederic Middlebrook Richards
Publisher : Unknown
Page : 334 pages
File Size : 51,6 Mb
Release : 1995
Category : Science
ISBN : 0120342464

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Protein Stability by David S. Eisenberg,Christian B. Anfinsen,Frederic Middlebrook Richards Pdf

The topics covered by this volume include: protein destabilization at low temperatures; engineering the stability and function of Gene V Protein; free energy balance in protein folding; modelling protein stability as a heteropolymer collapse; stability of alpha helices; protein stability with T4 Lysozyme.

Author : Yoshiaki Nosoh,Takeshi Sekiguchi
Publisher : Ellis Horwood
Page : 234 pages
File Size : 47,8 Mb
Release : 1991
Category : Science
ISBN : UOM:39015021637916

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Protein Stability and Stabilization Through Protein Engineering by Yoshiaki Nosoh,Takeshi Sekiguchi Pdf

Author : Kenneth M.Jr. Merz,Scott M. LeGrand
Publisher : Springer Science & Business Media
Page : 585 pages
File Size : 47,8 Mb
Release : 2012-12-06
Category : Science
ISBN : 9781468468311

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The Protein Folding Problem and Tertiary Structure Prediction by Kenneth M.Jr. Merz,Scott M. LeGrand Pdf

A solution to the protein folding problem has eluded researchers for more than 30 years. The stakes are high. Such a solution will make 40,000 more tertiary structures available for immediate study by translating the DNA sequence information in the sequence databases into three-dimensional protein structures. This translation will be indispensable for the analy sis of results from the Human Genome Project, de novo protein design, and many other areas of biotechnological research. Finally, an in-depth study of the rules of protein folding should provide vital clues to the protein fold ing process. The search for these rules is therefore an important objective for theoretical molecular biology. Both experimental and theoretical ap proaches have been used in the search for a solution, with many promising results but no general solution. In recent years, there has been an exponen tial increase in the power of computers. This has triggered an incredible outburst of theoretical approaches to solving the protein folding problem ranging from molecular dynamics-based studies of proteins in solution to the actual prediction of protein structures from first principles. This volume attempts to present a concise overview of these advances. Adrian Roitberg and Ron Elber describe the locally enhanced sam pling/simulated annealing conformational search algorithm (Chapter 1), which is potentially useful for the rapid conformational search of larger molecular systems.