Recombinant Protein Production With Prokaryotic And Eukaryotic Cells A Comparative View On Host Physiology

Author : Otto-Wilhelm Merten,D. Mattanovich,C. Lang,G. Larsson,P. Neubauer,D. Porro,P. Postma,J. Teixeira de Mattos,J.A. Cole
Publisher : Springer Science & Business Media
Page : 396 pages
File Size : 48,9 Mb
Release : 2013-04-17
Category : Science
ISBN : 9789401597494

Get Book

Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology by Otto-Wilhelm Merten,D. Mattanovich,C. Lang,G. Larsson,P. Neubauer,D. Porro,P. Postma,J. Teixeira de Mattos,J.A. Cole Pdf

More then 20 years have passed now since the first recombinant protein producing microorganisms have been developed. In the meanwhile, numerous proteins have been produced in bacteria, yeasts and filamentous fungi, as weIl as higher eukaryotic cells, and even entire plants and animals. Many recombinant proteins are on the market today, and some of them reached substantial market volumes. On the first sight one would expect the technology - including the physiology of the host strains - to be optimised in detail after a 20 year's period of development. However, several constraints have limited the incentive for optimisation, especially in the pharmaceutical industry like the urge to proceed quickly or the requirement to define the production parameters for registration early in the development phase. The additional expenses for registration of a new production strain often prohibits a change to an optimised strain. A continuous optimisation of the entire production process is not feasible for the same reasons.

Author : Otto-Wilhelm Merten
Publisher : Springer Science & Business Media
Page : 434 pages
File Size : 51,8 Mb
Release : 2001-11-30
Category : Medical
ISBN : 0792371372

Get Book

Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology by Otto-Wilhelm Merten Pdf

The general field of fundamental and applied biotechnology becomes increasingly important for the production of biologicals for human and veterinary use, by using prokaryotic and eukaryotic microorganisms. The papers in the present book are refereed articles compiled from oral and poster presentations from the EFB Meeting on Recombinant Protein Production with Prokaryotic and Eukaryotic Cells. A Comparative View on Host Physiology, which was organized in Semmering/A from 5th to 8th October 2000. A special feature of this meeting was the comparison of different classes of host cells, mainly bacteria, yeasts, filamentous fungi, and animal cells, which made obvious that many physiological features of recombinant protein formation, like cell nutrition, stress responses, protein folding and secretion, or genetic stability, follow similar patterns in different expression systems. This comparative aspect is by far the point of most interest because such comparisons are rarely done, and if they are done, their results are most often kept secret by the companies who generated them. Audience: Presently, a comparable book does not exist because the compiling of manuscripts from all fields of biotechnology (prokaryotic as well as eukaryotic, up to animal cell biotechnology) is not done in general. This particularity makes this book very interesting for postgraduate students and professionals in the large field of biotechnology who want to get a more global view on the current state of the expression of recombinant biologicals in different host cell systems, the physiological problems associated with the use of different expression systems, potential approaches to solve such difficulties by metabolic engineering or the use of other host cells, and the cooperation between process development and strain improvement, which is crucial for the optimisation of both the production strain and the process. This book should be in every library of an institution/organization involved in biotechnology.

Author : Basanta Kumara Behera
Publisher : CRC Press
Page : 300 pages
File Size : 46,6 Mb
Release : 2020-12-07
Category : Medical
ISBN : 9781351013130

Get Book

Biopharmaceuticals by Basanta Kumara Behera Pdf

Biopharmaceuticals: Challenges and Opportunities This book highlights how the traditional microbial process technology has been upgraded for the production of biologic drugs how manufacturing processes have evolved to meet the global market demand with quality products under the guidelines of internally recognized regulatory bodies. It also carries information on how, armed with a deeper understanding of life-threatening diseases, biopharmaceutical companies and the life sciences industry have developed formal and informal partnerships with researchers in institutes, universities, and other R&D organizations to fulfil timely, quality production with perfect safety and security. One of the most interesting aspects of this book is the conceptual development of personalized medicine (or precision medicine) to provide the right treatment to the right patient, at the right dose at an earlier stage of development, for genetic diseases. Besides this, it also highlights the most challenging aspects of modern biopharmaceutical science, focusing on the hot topics such as design and development of biologic drugs; the use of diversified groups of host cells belonging to animals, plants, microbes, insects, and mammals; stem cell therapy and gene therapy; supply chain management of biopharmaceuticals; and the future scope of biopharmaceutical industry development. This book is the latest resource for a wide circle of scientists, students, and researchers involved in understanding and implementing the knowledge of biopharmaceuticals to develop life-saving biologic drugs and to bring awareness to the development of personalized treatment that can potentially offer patients a faster diagnosis, fewer side effects, and better outcomes. Features: Explains how the traditional cell culture methodology has been changed to a fully continuous or partially continuous process Explains how to design and fabricate living organs of body by 3D bioprinting technology Focuses on how a biopharmaceutical company deals with various problems of regulatory bodies and develops innovative biologic drugs Narrates in detail the updated information on stem cell therapy and gene therapy Explains the development strategies and clinical significance of biosimilars and biobetters Highlights the supply chain management of biopharmaceuticals

Author : Eriola Betiku
Publisher : Cuvillier Verlag
Page : 155 pages
File Size : 44,9 Mb
Release : 2005
Category : Electronic
ISBN : 9783865373670

Get Book

Heterologous Production of the Human Basic Fibroblast Growth Factor and the Glucosyltransferase-S in Escherichia coli with and without Coproduction of Molecular Chaperones by Eriola Betiku Pdf

Author : Gary Walsh
Publisher : John Wiley & Sons
Page : 499 pages
File Size : 46,8 Mb
Release : 2013-04-25
Category : Science
ISBN : 9781118685754

Get Book

Pharmaceutical Biotechnology by Gary Walsh Pdf

Pharmaceutical Biotechnology offers students taking Pharmacy and related Medical and Pharmaceutical courses a comprehensive introduction to the fast-moving area of biopharmaceuticals. With a particular focus on the subject taken from a pharmaceutical perspective, initial chapters offer a broad introduction to protein science and recombinant DNA technology- key areas that underpin the whole subject. Subsequent chapters focus upon the development, production and analysis of these substances. Finally the book moves on to explore the science, biotechnology and medical applications of specific biotech products categories. These include not only protein-based substances but also nucleic acid and cell-based products. introduces essential principles underlining modern biotechnology- recombinant DNA technology and protein science an invaluable introduction to this fast-moving subject aimed specifically at pharmacy and medical students includes specific ‘product category chapters’ focusing on the pharmaceutical, medical and therapeutic properties of numerous biopharmaceutical products. entire chapter devoted to the principles of genetic engineering and how these drugs are developed. includes numerous relevant case studies to enhance student understanding no prior knowledge of protein structure is assumed

Author : Sven-Olof Enfors
Publisher : Springer Science & Business Media
Page : 264 pages
File Size : 54,7 Mb
Release : 2004-05-06
Category : Medical
ISBN : 3540203117

Get Book

Physiological Stress Responses in Bioprocesses by Sven-Olof Enfors Pdf

This review series covers trends in modern biotechnology. All aspects of this interdisciplinary technology, where knowledge, methods and expertise are required from chemistry, biochemistry, microbiology, genetics, chemical engineering and computer science, are treated. Electronic version available at http://link.springer.de/series/abe/

Author : Monika Schmoll,Christoph Dattenböck
Publisher : Springer
Page : 499 pages
File Size : 46,7 Mb
Release : 2016-04-04
Category : Science
ISBN : 9783319279510

Get Book

Gene Expression Systems in Fungi: Advancements and Applications by Monika Schmoll,Christoph Dattenböck Pdf

Biotechnology has emerged as one of the key environmentally safe technologies for the future which enables use of biomass to develop novel smart materials and to replace oil derived products. Fungi are the most efficient producers of the enzymes needed for this purpose and in addition they produce a plethora of secondary metabolites, among which novel antibiotics can be found. Industrial application and exploitation of the metabolic capacities of fungi requires highly productive and robust gene expression systems, which can be achieved by selection of appropriate species and strain improvement. In this book we aim to summarize homologous and heterologous gene expression systems of fungi for production of enzymes and secondary metabolites. A broad overview on requirements, challenges and successful applications shall serve as a basis for further development of fungi as biotechnological workhorses in research and industry.

Author : Fernanda Mozzi,R?ul R. Raya,Graciela M. Vignolo
Publisher : John Wiley & Sons
Page : 417 pages
File Size : 49,9 Mb
Release : 2010-01-29
Category : Technology & Engineering
ISBN : 9780813820958

Get Book

Biotechnology of Lactic Acid Bacteria by Fernanda Mozzi,R?ul R. Raya,Graciela M. Vignolo Pdf

This title represents a broad review of current research on LAB and their novel applications with contributions from a number of well-known leading scientists. The book encompasses a wide range of topics including both traditional and novel developing fields, and provides unparalleled, comprehensive information on new advances of genomics, proteomics, metabolism and biodiversity of LAB. Chapters contain state-of-the-art discussions of specific LAB applications such as their use as probiotics, live vaccines and starter cultures in old and new fermented products. The safety of these microorganisms and their interactions with diverse ecosystems natural biota are also covered as well as the new applications of well-known (bacteriocins) and novel (vitamins, low-calorie sugars, etc.) metabolites produced by LAB. This book is an essential reference for established researchers and scientists, doctoral and post-doctoral students, university professors and instructors, and food technologists working on food microbiology, physiology and biotechnology of lactic acid bacteria.

Author : Jessup M. Shively
Publisher : Springer Science & Business Media
Page : 353 pages
File Size : 55,9 Mb
Release : 2006-05-04
Category : Science
ISBN : 9783540337744

Get Book

Inclusions in Prokaryotes by Jessup M. Shively Pdf

The new series "Microbiology Monographs" begins with two volumes on intracellular components in prokaryotes. In this first volume, "Inclusions in Prokaryotes", the components, labeled inclusions, are defined as discrete bodies resulting from synthesis of a metabolic product. Research on the biosynthesis and reutilization of the accumulated materials is still in progress, and interest in the inclusions is growing. This comprehensive volume provides historical background and comprehensive reviews of eight well-known prokaryotic inclusions.

Author : Robert K. Poole
Publisher : Elsevier
Page : 673 pages
File Size : 47,6 Mb
Release : 2011-09-02
Category : Science
ISBN : 9780080560441

Get Book

Globins and Other Nitric Oxide-Reactive Proteins, Part A by Robert K. Poole Pdf

The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with over 400 volumes (all of them still in print), the series contains much material still relevant today—truly an essential publication for researchers in all fields of life sciences. Methods in Enzymology is now available online at ScienceDirect — full-text online of volumes 1 onwards. For more information about the Elsevier Book Series on ScienceDirect Program, please visit: http://www.info.sciencedirect.com/bookseries/This volume features methods for the study of globin and other nitric oxide-reactive proteins.

Author : Kai Wang
Publisher : Unknown
Page : 208 pages
File Size : 44,8 Mb
Release : 2006
Category : Bacterial genetics
ISBN : MSU:31293028450272

Get Book

Physiological Studies of Recombinant Protein Expression in Escherichia Coli by Kai Wang Pdf

Author : Manfred Zinn,Thomas Egli,Christoph Herwig,Atul Narang
Publisher : Frontiers Media SA
Page : 133 pages
File Size : 40,9 Mb
Release : 2021-03-30
Category : Science
ISBN : 9782889666492

Get Book

Recent Advances in Continuous Cultivation by Manfred Zinn,Thomas Egli,Christoph Herwig,Atul Narang Pdf

Author : Sorin Sonea,Léo G. Mathieu
Publisher : PUM
Page : 107 pages
File Size : 50,6 Mb
Release : 2000
Category : Reference
ISBN : 9782760617568

Get Book

Prokaryotology by Sorin Sonea,Léo G. Mathieu Pdf

Prokaryotes are profoundly original, highly efficient microorganisms that have played a decisive role in the evolution of life on Earth. Although disjunct, taken together their cells form one global superorganism or biological system. One of the results of their non-Darwinian evolution has been the development of enormous diversity and bio-energetic variety. Prokaryotic cells possess standardized mechanisms for easy gene exchanges (lateral gene transfer) and they can behave like receiving and broadcasting stations for genetic material. Ultimately, the result is a global communication system based on the prokaryotic hereditary patrimony, by analogy, a two-billion-year-old world wide web for their benefit. Eukaryotes have evolved from the association of at least three complementary prokaryotic cells, and their subsequent development has been enriched and accelerated by symbioses with other prokaryotes. One of these symbioses was responsible for the origin of vascular plants which transformed vast sections of the continental surface of the Earth from deserts to areas with luxuriant, life-supporting vegetation. All forms of life on our planet are directly or indirectly sustained and enriched by the positive contribution of prokaryotes. Sorin Sonea and L�o G. Mathieu have been professors at the Department of Microbiology and Immunology (Faculty of Medicine) at the Universit� de Montr�al. They have long been advocates of the ideas presented in this book.

Author : Kazumi Yagasaki,Yutaka Miura,Makoto Hatori,Yoshihiro Nomura
Publisher : Springer Science & Business Media
Page : 540 pages
File Size : 43,5 Mb
Release : 2004-05-19
Category : Science
ISBN : 140201970X

Get Book

Animal Cell Technology: Basic & Applied Aspects by Kazumi Yagasaki,Yutaka Miura,Makoto Hatori,Yoshihiro Nomura Pdf

Animal cell technology is a growing discipline of cell biology which aims not only to understand structures, functions and behaviors of differentiated animal cells, but also to ascertain their abilities to be used in industrial and medical purposes. The goal of animal cell technology includes accomplishments of clonal expansion of differentiated cells with useful ability, optimization of their culture conditions, modulation of their ability for production of medically and pharmaceutically important proteins and the application of animal cells to gene therapy, artificial organs and functional foods. This volume gives the readers a complete review of present state-of-the-art in Japan and other countries where this field is well advanced. The Proceedings will be useful for the cell biologists, biochemists, molecular biologists, immunologists, biochemical engineers and other disciplines related to animal cell culture, working in either academic environments or in industries of biotechnology and pharmacy.

Author : Paul Pumpens
Publisher : CRC Press
Page : 616 pages
File Size : 41,9 Mb
Release : 2020-02-03
Category : Science
ISBN : 9780429672903

Get Book

Single-stranded RNA phages by Paul Pumpens Pdf

This is a comprehensive guide to single-stranded RNA phages (family Leviviridae), first discovered in 1961. These phages played a unique role in early studies of molecular biology, the genetic code, translation, replication, suppression of mutations. Special attention is devoted to modern applications of the RNA phages and their products in nanotechnology, vaccinology, gene discovery, evolutionary and environmental studies. Included is an overview of the generation of novel vaccines, gene therapy vectors, drug delivery, and diagnostic tools exploring the role of RNA phage-derived products in the revolutionary progress of the protein tethering and bioimaging protocols. Key Features Presents the first full guide to single-stranded RNA phages Reviews the history of molecular biology summarizing the role RNA phages in the development of the life sciences Demonstrates how RNA phage-derived products have resulted in nanotechnological applications Presents an up-to-date account of the role played by RNA phages in evolutionary and environmental studies